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Q02899

- OYE1_SACPS

UniProt

Q02899 - OYE1_SACPS

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Protein
NADPH dehydrogenase 1
Gene
OYE1
Organism
Saccharomyces pastorianus (Lager yeast) (Saccharomyces cerevisiae x Saccharomyces eubayanus)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Oxidizes beta-NADH, beta-NADPH, and alpha-NADPH.

Catalytic activityi

NADPH + acceptor = NADP+ + reduced acceptor.

Cofactori

FMN.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381FMN
Binding sitei192 – 1921FMN
Binding sitei192 – 1921Substrate
Binding sitei195 – 1951Substrate
Active sitei197 – 1971Proton donor By similarity
Binding sitei244 – 2441FMN
Binding sitei349 – 3491FMN
Binding sitei376 – 3761Substrate

GO - Molecular functioni

  1. FMN binding Source: InterPro
  2. NADPH dehydrogenase activity Source: UniProtKB-EC
  3. pentaerythritol trinitrate reductase activity Source: UniProtKB-EC
  4. trichloro-p-hydroquinone reductive dehalogenase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Flavoprotein, FMN, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADPH dehydrogenase 1 (EC:1.6.99.1)
    Alternative name(s):
    Old yellow enzyme 1
    Gene namesi
    Name:OYE1
    OrganismiSaccharomyces pastorianus (Lager yeast) (Saccharomyces cerevisiae x Saccharomyces eubayanus)
    Taxonomic identifieri27292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi115 – 1151Q → N: Strong reduction of substrate binding.
    Mutagenesisi192 – 1921H → N: Strong reduction of substrate binding. Reduced oxidation of NADPH. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 400399NADPH dehydrogenase 1
    PRO_0000194473Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer or heterodimer.

    Structurei

    Secondary structure

    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi16 – 183
    Beta strandi21 – 233
    Beta strandi26 – 349
    Turni44 – 474
    Turni51 – 533
    Helixi54 – 618
    Beta strandi68 – 703
    Beta strandi74 – 774
    Helixi78 – 803
    Beta strandi88 – 914
    Helixi92 – 10716
    Beta strandi111 – 1177
    Helixi120 – 1223
    Helixi125 – 1306
    Beta strandi136 – 1383
    Beta strandi140 – 1423
    Helixi146 – 1549
    Beta strandi159 – 1613
    Helixi164 – 18320
    Beta strandi187 – 1926
    Helixi198 – 2036
    Turni205 – 2073
    Beta strandi215 – 2173
    Helixi218 – 2214
    Helixi223 – 23614
    Helixi238 – 2403
    Beta strandi241 – 2455
    Turni251 – 2533
    Helixi256 – 2583
    Helixi262 – 27817
    Beta strandi284 – 2896
    Beta strandi296 – 2983
    Turni300 – 3034
    Helixi312 – 3165
    Beta strandi321 – 3266
    Helixi331 – 3377
    Beta strandi343 – 3464
    Helixi349 – 3535
    Helixi357 – 3637
    Helixi372 – 3743
    Beta strandi378 – 3803
    Turni381 – 3833
    Helixi389 – 3946
    Helixi397 – 3993

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BWKX-ray2.30A2-399[»]
    1BWLX-ray2.70A2-399[»]
    1K02X-ray2.70A2-400[»]
    1K03X-ray2.70A2-400[»]
    1OYAX-ray2.00A1-400[»]
    1OYBX-ray2.00A1-400[»]
    1OYCX-ray2.00A1-400[»]
    3RNDX-ray1.40A2-400[»]
    3TX9X-ray2.00A1-400[»]
    3TXZX-ray1.70A1-400[»]
    4GBUX-ray1.18A1-400[»]
    4GE8X-ray1.50A1-400[»]
    4GWEX-ray1.45A1-400[»]
    4GXMX-ray1.36A1-400[»]
    4H4IX-ray1.25A1-400[»]
    4H6KX-ray1.55A2-400[»]
    4K7VX-ray1.52A1-400[»]
    4K7YX-ray1.20A1-400[»]
    4K8EX-ray1.27A1-400[»]
    4K8HX-ray1.55A1-400[»]
    ProteinModelPortaliQ02899.
    SMRiQ02899. Positions 2-400.

    Miscellaneous databases

    EvolutionaryTraceiQ02899.

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR001155. OxRdtase_FMN_N.
    [Graphical view]
    PfamiPF00724. Oxidored_FMN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q02899-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSFVKDFKPQ ALGDTNLFKP IKIGNNELLH RAVIPPLTRM RALHPGNIPN    50
    RDWAVEYYTQ RAQRPGTMII TEGAFISPQA GGYDNAPGVW SEEQMVEWTK 100
    IFNAIHEKKS FVWVQLWVLG WAAFPDNLAR DGLRYDSASD NVFMDAEQEA 150
    KAKKANNPQH SLTKDEIKQY IKEYVQAAKN SIAAGADGVE IHSANGYLLN 200
    QFLDPHSNTR TDEYGGSIEN RARFTLEVVD ALVEAIGHEK VGLRLSPYGV 250
    FNSMSGGAET GIVAQYAYVA GELEKRAKAG KRLAFVHLVE PRVTNPFLTE 300
    GEGEYEGGSN DFVYSIWKGP VIRAGNFALH PEVVREEVKD KRTLIGYGRF 350
    FISNPDLVDR LEKGLPLNKY DRDTFYQMSA HGYIDYPTYE EALKLGWDKK 400
    Length:400
    Mass (Da):45,015
    Last modified:January 23, 2007 - v3
    Checksum:i0F3CA987E3EE1310
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53597 Genomic DNA. Translation: CAA37666.1.
    PIRiA39495.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53597 Genomic DNA. Translation: CAA37666.1 .
    PIRi A39495.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BWK X-ray 2.30 A 2-399 [» ]
    1BWL X-ray 2.70 A 2-399 [» ]
    1K02 X-ray 2.70 A 2-400 [» ]
    1K03 X-ray 2.70 A 2-400 [» ]
    1OYA X-ray 2.00 A 1-400 [» ]
    1OYB X-ray 2.00 A 1-400 [» ]
    1OYC X-ray 2.00 A 1-400 [» ]
    3RND X-ray 1.40 A 2-400 [» ]
    3TX9 X-ray 2.00 A 1-400 [» ]
    3TXZ X-ray 1.70 A 1-400 [» ]
    4GBU X-ray 1.18 A 1-400 [» ]
    4GE8 X-ray 1.50 A 1-400 [» ]
    4GWE X-ray 1.45 A 1-400 [» ]
    4GXM X-ray 1.36 A 1-400 [» ]
    4H4I X-ray 1.25 A 1-400 [» ]
    4H6K X-ray 1.55 A 2-400 [» ]
    4K7V X-ray 1.52 A 1-400 [» ]
    4K7Y X-ray 1.20 A 1-400 [» ]
    4K8E X-ray 1.27 A 1-400 [» ]
    4K8H X-ray 1.55 A 1-400 [» ]
    ProteinModelPortali Q02899.
    SMRi Q02899. Positions 2-400.
    ModBasei Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q02899.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR001155. OxRdtase_FMN_N.
    [Graphical view ]
    Pfami PF00724. Oxidored_FMN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The cloning and expression of a gene encoding Old Yellow Enzyme from Saccharomyces carlsbergensis."
      Saito K., Thiele D.J., Davio M., Lockridge O., Massey V.
      J. Biol. Chem. 266:20720-20724(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-47.
      Strain: Brewer's bottom.
    2. "Old yellow enzyme at 2-A resolution: overall structure, ligand binding, and comparison with related flavoproteins."
      Fox K.M., Karplus P.A.
      Structure 2:1089-1105(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH FMN AND SUBSTRATE.
    3. "On the active site of Old Yellow Enzyme. Role of histidine 191 and asparagine 194."
      Brown B.J., Deng Z., Karplus P.A., Massey V.
      J. Biol. Chem. 273:32753-32762(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 1-399 IN COMPLEX WITH FMN, MUTAGENESIS OF HIS-192.
    4. Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 1-400 OF MUTANT ASN-115 IN COMPLEX WITH FMN AND SUBSTRATE.

    Entry informationi

    Entry nameiOYE1_SACPS
    AccessioniPrimary (citable) accession number: Q02899
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: January 23, 2007
    Last modified: July 9, 2014
    This is version 86 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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