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Protein

NADPH dehydrogenase 1

Gene

OYE1

Organism
Saccharomyces pastorianus (Lager yeast) (Saccharomyces cerevisiae x Saccharomyces eubayanus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Oxidizes beta-NADH, beta-NADPH, and alpha-NADPH.

Catalytic activityi

NADPH + acceptor = NADP+ + reduced acceptor.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38FMN3 Publications1
Binding sitei192FMN3 Publications1
Binding sitei192Substrate2 Publications1
Binding sitei195Substrate2 Publications1
Active sitei197Proton donorBy similarity1
Binding sitei244FMN3 Publications1
Binding sitei349FMN3 Publications1
Binding sitei376Substrate2 Publications1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Flavoprotein, FMN, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH dehydrogenase 1 (EC:1.6.99.1)
Alternative name(s):
Old yellow enzyme 1
Gene namesi
Name:OYE1
OrganismiSaccharomyces pastorianus (Lager yeast) (Saccharomyces cerevisiae x Saccharomyces eubayanus)
Taxonomic identifieri27292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi115Q → N: Strong reduction of substrate binding. 1
Mutagenesisi192H → N: Strong reduction of substrate binding. Reduced oxidation of NADPH. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001944732 – 400NADPH dehydrogenase 1Add BLAST399

Interactioni

Subunit structurei

Homodimer or heterodimer.3 Publications

Structurei

Secondary structure

1400
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 6Combined sources4
Beta strandi13 – 15Combined sources3
Helixi16 – 18Combined sources3
Beta strandi21 – 23Combined sources3
Beta strandi26 – 34Combined sources9
Turni44 – 47Combined sources4
Turni51 – 53Combined sources3
Helixi54 – 61Combined sources8
Beta strandi68 – 70Combined sources3
Beta strandi74 – 77Combined sources4
Helixi78 – 80Combined sources3
Beta strandi88 – 91Combined sources4
Helixi92 – 107Combined sources16
Beta strandi111 – 117Combined sources7
Helixi120 – 122Combined sources3
Helixi125 – 130Combined sources6
Beta strandi136 – 138Combined sources3
Beta strandi140 – 142Combined sources3
Helixi146 – 154Combined sources9
Beta strandi159 – 161Combined sources3
Helixi164 – 183Combined sources20
Beta strandi187 – 192Combined sources6
Helixi198 – 203Combined sources6
Turni205 – 207Combined sources3
Beta strandi215 – 217Combined sources3
Helixi218 – 221Combined sources4
Helixi223 – 236Combined sources14
Helixi238 – 240Combined sources3
Beta strandi241 – 245Combined sources5
Turni251 – 253Combined sources3
Helixi256 – 258Combined sources3
Helixi262 – 278Combined sources17
Beta strandi284 – 289Combined sources6
Beta strandi296 – 298Combined sources3
Turni300 – 303Combined sources4
Helixi312 – 316Combined sources5
Beta strandi321 – 326Combined sources6
Helixi331 – 337Combined sources7
Beta strandi343 – 346Combined sources4
Helixi349 – 353Combined sources5
Helixi357 – 363Combined sources7
Helixi372 – 374Combined sources3
Beta strandi378 – 380Combined sources3
Turni381 – 383Combined sources3
Helixi389 – 394Combined sources6
Helixi397 – 399Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BWKX-ray2.30A2-399[»]
1BWLX-ray2.70A2-399[»]
1K02X-ray2.70A2-400[»]
1K03X-ray2.70A2-400[»]
1OYAX-ray2.00A1-400[»]
1OYBX-ray2.00A1-400[»]
1OYCX-ray2.00A1-400[»]
3RNDX-ray1.40A2-400[»]
3TX9X-ray2.00A1-400[»]
3TXZX-ray1.70A1-400[»]
4GBUX-ray1.18A1-400[»]
4GE8X-ray1.50A1-400[»]
4GWEX-ray1.45A1-400[»]
4GXMX-ray1.36A1-400[»]
4H4IX-ray1.25A1-400[»]
4H6KX-ray1.55A2-400[»]
4K7VX-ray1.52A1-400[»]
4K7YX-ray1.20A1-400[»]
4K8EX-ray1.27A1-400[»]
4K8HX-ray1.55A1-400[»]
4RNUX-ray2.68A/B/C/D2-302[»]
4RNVX-ray2.47A/B/C/D2-302[»]
4RNWX-ray1.55A/B2-292[»]
4RNXX-ray1.25A/B2-397[»]
4YILX-ray1.46A2-398[»]
4YNCX-ray1.50A2-398[»]
ProteinModelPortaliQ02899.
SMRiQ02899.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ02899.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001155. OxRdtase_FMN_N.
[Graphical view]
PfamiPF00724. Oxidored_FMN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02899-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFVKDFKPQ ALGDTNLFKP IKIGNNELLH RAVIPPLTRM RALHPGNIPN
60 70 80 90 100
RDWAVEYYTQ RAQRPGTMII TEGAFISPQA GGYDNAPGVW SEEQMVEWTK
110 120 130 140 150
IFNAIHEKKS FVWVQLWVLG WAAFPDNLAR DGLRYDSASD NVFMDAEQEA
160 170 180 190 200
KAKKANNPQH SLTKDEIKQY IKEYVQAAKN SIAAGADGVE IHSANGYLLN
210 220 230 240 250
QFLDPHSNTR TDEYGGSIEN RARFTLEVVD ALVEAIGHEK VGLRLSPYGV
260 270 280 290 300
FNSMSGGAET GIVAQYAYVA GELEKRAKAG KRLAFVHLVE PRVTNPFLTE
310 320 330 340 350
GEGEYEGGSN DFVYSIWKGP VIRAGNFALH PEVVREEVKD KRTLIGYGRF
360 370 380 390 400
FISNPDLVDR LEKGLPLNKY DRDTFYQMSA HGYIDYPTYE EALKLGWDKK
Length:400
Mass (Da):45,015
Last modified:January 23, 2007 - v3
Checksum:i0F3CA987E3EE1310
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53597 Genomic DNA. Translation: CAA37666.1.
PIRiA39495.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53597 Genomic DNA. Translation: CAA37666.1.
PIRiA39495.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BWKX-ray2.30A2-399[»]
1BWLX-ray2.70A2-399[»]
1K02X-ray2.70A2-400[»]
1K03X-ray2.70A2-400[»]
1OYAX-ray2.00A1-400[»]
1OYBX-ray2.00A1-400[»]
1OYCX-ray2.00A1-400[»]
3RNDX-ray1.40A2-400[»]
3TX9X-ray2.00A1-400[»]
3TXZX-ray1.70A1-400[»]
4GBUX-ray1.18A1-400[»]
4GE8X-ray1.50A1-400[»]
4GWEX-ray1.45A1-400[»]
4GXMX-ray1.36A1-400[»]
4H4IX-ray1.25A1-400[»]
4H6KX-ray1.55A2-400[»]
4K7VX-ray1.52A1-400[»]
4K7YX-ray1.20A1-400[»]
4K8EX-ray1.27A1-400[»]
4K8HX-ray1.55A1-400[»]
4RNUX-ray2.68A/B/C/D2-302[»]
4RNVX-ray2.47A/B/C/D2-302[»]
4RNWX-ray1.55A/B2-292[»]
4RNXX-ray1.25A/B2-397[»]
4YILX-ray1.46A2-398[»]
4YNCX-ray1.50A2-398[»]
ProteinModelPortaliQ02899.
SMRiQ02899.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ02899.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001155. OxRdtase_FMN_N.
[Graphical view]
PfamiPF00724. Oxidored_FMN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOYE1_SACPS
AccessioniPrimary (citable) accession number: Q02899
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 95 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.