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Protein

Medium-chain fatty acid ethyl ester synthase/esterase 1

Gene

EEB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Displays enzymatic activity both for medium-chain fatty acid (MCFA) ethyl ester synthesis and hydrolysis (esterase activity). MCFA are toxic for yeast and this enzyme could thus be involved in their detoxification by esterification.1 Publication

Catalytic activityi

Acetyl-CoA + an alcohol = CoA + an acetyl ester.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei251 – 2511Charge relay systemBy similarity
Active sitei399 – 3991Charge relay systemBy similarity
Active sitei428 – 4281Charge relay systemBy similarity

GO - Molecular functioni

  • alcohol O-acetyltransferase activity Source: UniProtKB-EC
  • alcohol O-octanoyltransferase activity Source: SGD
  • short-chain carboxylesterase activity Source: SGD

GO - Biological processi

  • medium-chain fatty acid biosynthetic process Source: SGD
  • medium-chain fatty acid catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Hydrolase, Serine esterase, Transferase

Enzyme and pathway databases

BioCyciYEAST:G3O-33999-MONOMER.
ReactomeiR-SCE-1483191. Synthesis of PC.

Protein family/group databases

ESTHERiyeast-MCFS1. abh_upf0017.

Names & Taxonomyi

Protein namesi
Recommended name:
Medium-chain fatty acid ethyl ester synthase/esterase 1
Alternative name(s):
Alcohol O-acetyltransferase (EC:2.3.1.84, EC:3.1.1.-)
Ethyl ester biosynthesis protein 1
Gene namesi
Name:EEB1
Ordered Locus Names:YPL095C
ORF Names:LPG13C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL095C.
SGDiS000006016. EEB1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 456456Medium-chain fatty acid ethyl ester synthase/esterase 1PRO_0000212456Add
BLAST

Interactioni

Protein-protein interaction databases

BioGridi36086. 11 interactions.
DIPiDIP-2691N.
IntActiQ02891. 4 interactions.
MINTiMINT-495415.

Structurei

3D structure databases

ProteinModelPortaliQ02891.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00530000062981.
HOGENOMiHOG000248589.
InParanoidiQ02891.
KOiK07019.
OMAiNDEKATH.
OrthoDBiEOG7X9GHP.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000952. AB_hydrolase_4_CS.
IPR012020. ABHD4.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005211. Ab_hydro_YheT. 1 hit.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS01133. UPF0017. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q02891-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRSGYYPTV TPSHWGYNGT VKHVLGEKGT KSLAFRDSKR QIPLHEFVTK
60 70 80 90 100
HVPTLKDGAN FRLNSLLFTG YLQTLYLSAG DFSKKFQVFY GREIIKFSDG
110 120 130 140 150
GVCTADWVMP EWEQTYSLNA EKASFNEKQF SNDEKATHPK GWPRLHPRTR
160 170 180 190 200
YLSSEELEKC HSKGYSYPLV VVLHGLAGGS HEPLIRALSE DLSKVGDGKF
210 220 230 240 250
QVVVLNARGC SRSKVTTRRI FTALHTGDVR EFLNHQKALF PQRKIYAVGT
260 270 280 290 300
SFGAAMLTNY LGEEGDNCPL NAAVALSNPW DFVHTWDKLA HDWWSNHIFS
310 320 330 340 350
RTLTQFLTRT VKVNMNELQV PENFEVSHKP TVEKPVFYTY TRENLEKAEK
360 370 380 390 400
FTDILEFDNL FTAPSMGLPD GLTYYRKASS INRLPNIKIP TLIINATDDP
410 420 430 440 450
VTGENVIPYK QARENPCVLL CETDLGGHLA YLDNESNSWL TKQAAEFLGS

FDELVL
Length:456
Mass (Da):51,724
Last modified:November 1, 1997 - v1
Checksum:iCABC660FF44A287E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43281 Genomic DNA. Translation: AAB68204.1.
AY692561 Genomic DNA. Translation: AAT92580.1.
BK006949 Genomic DNA. Translation: DAA11338.1.
PIRiS61971.
RefSeqiNP_015230.1. NM_001183909.1.

Genome annotation databases

EnsemblFungiiYPL095C; YPL095C; YPL095C.
GeneIDi856010.
KEGGisce:YPL095C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43281 Genomic DNA. Translation: AAB68204.1.
AY692561 Genomic DNA. Translation: AAT92580.1.
BK006949 Genomic DNA. Translation: DAA11338.1.
PIRiS61971.
RefSeqiNP_015230.1. NM_001183909.1.

3D structure databases

ProteinModelPortaliQ02891.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36086. 11 interactions.
DIPiDIP-2691N.
IntActiQ02891. 4 interactions.
MINTiMINT-495415.

Protein family/group databases

ESTHERiyeast-MCFS1. abh_upf0017.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL095C; YPL095C; YPL095C.
GeneIDi856010.
KEGGisce:YPL095C.

Organism-specific databases

EuPathDBiFungiDB:YPL095C.
SGDiS000006016. EEB1.

Phylogenomic databases

GeneTreeiENSGT00530000062981.
HOGENOMiHOG000248589.
InParanoidiQ02891.
KOiK07019.
OMAiNDEKATH.
OrthoDBiEOG7X9GHP.

Enzyme and pathway databases

BioCyciYEAST:G3O-33999-MONOMER.
ReactomeiR-SCE-1483191. Synthesis of PC.

Miscellaneous databases

PROiQ02891.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000952. AB_hydrolase_4_CS.
IPR012020. ABHD4.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005211. Ab_hydro_YheT. 1 hit.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS01133. UPF0017. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "The Saccharomyces cerevisiae EHT1 and EEB1 genes encode novel enzymes with medium-chain fatty acid ethyl ester synthesis and hydrolysis capacity."
    Saerens S.M.G., Verstrepen K.J., Van Laere S.D., Voet A.R., Van Dijck P., Delvaux F.R., Thevelein J.M.
    J. Biol. Chem. 281:4446-4456(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiMCFS1_YEAST
AccessioniPrimary (citable) accession number: Q02891
Secondary accession number(s): D6W3S2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 8, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 573 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.