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Protein

Medium-chain fatty acid ethyl ester synthase/esterase 1

Gene

EEB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Displays enzymatic activity both for medium-chain fatty acid (MCFA) ethyl ester synthesis and hydrolysis (esterase activity). MCFA are toxic for yeast and this enzyme could thus be involved in their detoxification by esterification.1 Publication

Miscellaneous

Present with 573 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Acetyl-CoA + an alcohol = CoA + an acetyl ester.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei251Charge relay systemBy similarity1
Active sitei399Charge relay systemBy similarity1
Active sitei428Charge relay systemBy similarity1

GO - Molecular functioni

  • acylglycerol lipase activity Source: GO_Central
  • alcohol O-acetyltransferase activity Source: UniProtKB-EC
  • alcohol O-octanoyltransferase activity Source: SGD
  • short-chain carboxylesterase activity Source: SGD

GO - Biological processi

  • medium-chain fatty acid biosynthetic process Source: SGD
  • medium-chain fatty acid catabolic process Source: SGD

Keywordsi

Molecular functionAcyltransferase, Hydrolase, Serine esterase, Transferase

Enzyme and pathway databases

BioCyciYEAST:G3O-33999-MONOMER
ReactomeiR-SCE-1483191 Synthesis of PC

Protein family/group databases

ESTHERiyeast-MCFS1 abh_upf0017

Names & Taxonomyi

Protein namesi
Recommended name:
Medium-chain fatty acid ethyl ester synthase/esterase 1
Alternative name(s):
Alcohol O-acetyltransferase (EC:2.3.1.84, EC:3.1.1.-)
Ethyl ester biosynthesis protein 1
Gene namesi
Name:EEB1
Ordered Locus Names:YPL095C
ORF Names:LPG13C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL095C
SGDiS000006016 EEB1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002124561 – 456Medium-chain fatty acid ethyl ester synthase/esterase 1Add BLAST456

Proteomic databases

PaxDbiQ02891
PRIDEiQ02891

Interactioni

Protein-protein interaction databases

BioGridi36086, 72 interactors
DIPiDIP-2691N
IntActiQ02891, 2 interactors
MINTiQ02891
STRINGi4932.YPL095C

Structurei

3D structure databases

ProteinModelPortaliQ02891
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini168 – 434AB hydrolase-1Sequence analysisAdd BLAST267

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00530000062981
HOGENOMiHOG000248589
InParanoidiQ02891
KOiK07019
OrthoDBiEOG092C2YV9

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR000073 AB_hydrolase_1
IPR000952 AB_hydrolase_4_CS
IPR012020 ABHD4
PfamiView protein in Pfam
PF00561 Abhydrolase_1, 1 hit
PIRSFiPIRSF005211 Ab_hydro_YheT, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
PROSITEiView protein in PROSITE
PS01133 UPF0017, 1 hit

Sequencei

Sequence statusi: Complete.

Q02891-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRSGYYPTV TPSHWGYNGT VKHVLGEKGT KSLAFRDSKR QIPLHEFVTK
60 70 80 90 100
HVPTLKDGAN FRLNSLLFTG YLQTLYLSAG DFSKKFQVFY GREIIKFSDG
110 120 130 140 150
GVCTADWVMP EWEQTYSLNA EKASFNEKQF SNDEKATHPK GWPRLHPRTR
160 170 180 190 200
YLSSEELEKC HSKGYSYPLV VVLHGLAGGS HEPLIRALSE DLSKVGDGKF
210 220 230 240 250
QVVVLNARGC SRSKVTTRRI FTALHTGDVR EFLNHQKALF PQRKIYAVGT
260 270 280 290 300
SFGAAMLTNY LGEEGDNCPL NAAVALSNPW DFVHTWDKLA HDWWSNHIFS
310 320 330 340 350
RTLTQFLTRT VKVNMNELQV PENFEVSHKP TVEKPVFYTY TRENLEKAEK
360 370 380 390 400
FTDILEFDNL FTAPSMGLPD GLTYYRKASS INRLPNIKIP TLIINATDDP
410 420 430 440 450
VTGENVIPYK QARENPCVLL CETDLGGHLA YLDNESNSWL TKQAAEFLGS

FDELVL
Length:456
Mass (Da):51,724
Last modified:November 1, 1997 - v1
Checksum:iCABC660FF44A287E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43281 Genomic DNA Translation: AAB68204.1
AY692561 Genomic DNA Translation: AAT92580.1
BK006949 Genomic DNA Translation: DAA11338.1
PIRiS61971
RefSeqiNP_015230.1, NM_001183909.1

Genome annotation databases

EnsemblFungiiYPL095C; YPL095C; YPL095C
GeneIDi856010
KEGGisce:YPL095C

Similar proteinsi

Entry informationi

Entry nameiMCFS1_YEAST
AccessioniPrimary (citable) accession number: Q02891
Secondary accession number(s): D6W3S2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

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