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Protein

Autophagy-related protein 21

Gene

ATG21

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for cytoplasm to vacuole transport (Cvt) vesicles formation and mitophagy. Involved in binding of phosphatidylethanolamine to ATG8 and in recruitment of ATG8 and ATG5 to the pre-autophagosomal structure. Protects ATG8 from ARG4-mediated cleavage. Essential for maturation of proaminopeptidase I.9 Publications

Miscellaneous

Present with 6020 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • phosphatidylinositol-3,5-bisphosphate binding Source: SGD
  • phosphatidylinositol-3-phosphate binding Source: SGD
  • phosphatidylinositol-4-phosphate binding Source: SGD

GO - Biological processi

  • autophagy of mitochondrion Source: SGD
  • macroautophagy Source: SGD
  • piecemeal microautophagy of the nucleus Source: SGD
  • protein lipidation Source: SGD
  • protein localization by the Cvt pathway Source: SGD
  • protein localization to phagophore assembly site Source: SGD
  • protein transport Source: UniProtKB-KW
  • vesicle organization Source: SGD

Keywordsi

Biological processAutophagy, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-34003-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Autophagy-related protein 21
Alternative name(s):
Cytoplasm to vacuole transport protein 21
Homologous with SVP1 protein 1
Maturation of proaminopeptidase I protein 1
Gene namesi
Name:ATG21
Synonyms:CVT21, HSV1, MAI1
Ordered Locus Names:YPL100W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL100W
SGDiS000006021 ATG21

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Vacuole

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi343 – 344RR → KK: Loss of aminopeptidase I precursor maturation and no more association with vacuole and punctate structures. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000508821 – 496Autophagy-related protein 21Add BLAST496

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei213PhosphothreonineCombined sources1
Modified residuei237PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ02887
PaxDbiQ02887
PRIDEiQ02887

PTM databases

iPTMnetiQ02887

Interactioni

Protein-protein interaction databases

BioGridi36080, 134 interactors
IntActiQ02887, 7 interactors
MINTiQ02887
STRINGi4932.YPL100W

Structurei

3D structure databases

ProteinModelPortaliQ02887
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati294 – 334WD 1Add BLAST41
Repeati346 – 385WD 2Add BLAST40
Repeati448 – 488WD 3Add BLAST41

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi342 – 346FRRGT-motif5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi77 – 80Poly-Glu4

Domaini

Contains a beta-propeller domain involved in specific binding to phosphatidylinositol 3,5-bisphosphate (PIP2).
The FRRGT-motif is essential for the cytoplasm to vacuole transport (Cvt) pathway and for the recruitment of ATG8 and ATG16 to the PAS in nutrient-rich medium and in both its recruitment to and dissociation from the PAS under starvation conditions.

Sequence similaritiesi

Belongs to the WD repeat SVP1 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

HOGENOMiHOG000217543
InParanoidiQ02887
KOiK17908
OMAiNLIWEPP
OrthoDBiEOG092C3UKJ

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR036322 WD40_repeat_dom_sf
SMARTiView protein in SMART
SM00320 WD40, 3 hits
SUPFAMiSSF50978 SSF50978, 3 hits

Sequencei

Sequence statusi: Complete.

Q02887-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVLQFNQDA TCCVVAASSH QISIFNCDPF GKCFEIDTKN SKKKTSNNNG
60 70 80 90 100
SASNSESRNN EESILITNGS RDRTDAEEEE DNEDNALVTG NILKEGEFVI
110 120 130 140 150
EMLFSTSLIA IADRGQGLNK GKKLKIVNTK RKCTICEIVF PHEIVDVVMN
160 170 180 190 200
RKRMCVLLES DQIFIYDISC MKPLETIDLW EDHYKRSQAN SFSNASNTGT
210 220 230 240 250
LEGDSANLNR VATNLLANAT QKSVNGSNPS VRTRRNSLRS KIRPRMVLSN
260 270 280 290 300
DDRSILCFTA YSSPKKNKPN SEALYDVVIY DTLNVTPVNY LNSVHKGNVA
310 320 330 340 350
CLAVSHDGKL LATASDKGTI IRVFHTGVDS DYMSSRSLFK EFRRGTRLCN
360 370 380 390 400
LYQLAFDKSM TMIGCVGDTD TIHLFKLDDA SNSLPGDNSS NGHWNEEEYI
410 420 430 440 450
LASNSNPSMG TPKEIPLSKP RIANYFSKKI KSSIPNQNLS RNFAYITVNE
460 470 480 490
SNRSCLGFPD EFPNQVYIAS DDGTFSIYSI PSKPGECVLT KNNKFT
Length:496
Mass (Da):55,188
Last modified:November 1, 1996 - v1
Checksum:i4454C28180E3974D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43281 Genomic DNA Translation: AAB68199.1
BK006949 Genomic DNA Translation: DAA11332.1
PIRiS61966
RefSeqiNP_015225.1, NM_001183914.1

Genome annotation databases

EnsemblFungiiYPL100W; YPL100W; YPL100W
GeneIDi856004
KEGGisce:YPL100W

Similar proteinsi

Entry informationi

Entry nameiATG21_YEAST
AccessioniPrimary (citable) accession number: Q02887
Secondary accession number(s): D6W3R6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health