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Protein

Autophagy-related protein 21

Gene

ATG21

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for cytoplasm to vacuole transport (Cvt) vesicles formation and mitophagy. Involved in binding of phosphatidylethanolamine to ATG8 and in recruitment of ATG8 and ATG5 to the pre-autophagosomal structure. Protects ATG8 from ARG4-mediated cleavage. Essential for maturation of proaminopeptidase I.9 Publications

GO - Molecular functioni

GO - Biological processi

  • autophagosome assembly Source: GO_Central
  • CVT pathway Source: SGD
  • macroautophagy Source: SGD
  • mitophagy Source: SGD
  • piecemeal microautophagy of nucleus Source: SGD
  • protein lipidation Source: SGD
  • protein localization to pre-autophagosomal structure Source: SGD
  • vesicle organization Source: SGD
Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-34003-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Autophagy-related protein 21
Alternative name(s):
Cytoplasm to vacuole transport protein 21
Homologous with SVP1 protein 1
Maturation of proaminopeptidase I protein 1
Gene namesi
Name:ATG21
Synonyms:CVT21, HSV1, MAI1
Ordered Locus Names:YPL100W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL100W.
SGDiS000006021. ATG21.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • endosome Source: SGD
  • extrinsic component of membrane Source: GO_Central
  • fungal-type vacuole membrane Source: SGD
  • pre-autophagosomal structure membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Vacuole

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi343 – 3442RR → KK: Loss of aminopeptidase I precursor maturation and no more association with vacuole and punctate structures. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 496496Autophagy-related protein 21PRO_0000050882Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei213 – 2131PhosphothreonineCombined sources
Modified residuei237 – 2371PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ02887.

PTM databases

iPTMnetiQ02887.

Interactioni

Protein-protein interaction databases

BioGridi36080. 90 interactions.
IntActiQ02887. 3 interactions.
MINTiMINT-4504851.

Structurei

3D structure databases

ProteinModelPortaliQ02887.
SMRiQ02887. Positions 351-377.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati294 – 33441WD 1Add
BLAST
Repeati346 – 38540WD 2Add
BLAST
Repeati448 – 48841WD 3Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi342 – 3465FRRGT-motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi77 – 804Poly-Glu

Domaini

Contains a beta-propeller domain involved in specific binding to phosphatidylinositol 3,5-bisphosphate (PIP2).
The FRRGT-motif is essential for the cytoplasm to vacuole transport (Cvt) pathway and for the recruitment of ATG8 and ATG16 to the PAS in nutrient-rich medium and in both its recruitment to and dissociation from the PAS under starvation conditions.

Sequence similaritiesi

Belongs to the WD repeat SVP1 family.Curated
Contains 3 WD repeats.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

HOGENOMiHOG000217543.
InParanoidiQ02887.
OMAiPFGKCFE.
OrthoDBiEOG092C3UKJ.

Family and domain databases

Gene3Di2.130.10.10. 5 hits.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
SMARTiSM00320. WD40. 3 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 3 hits.

Sequencei

Sequence statusi: Complete.

Q02887-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVLQFNQDA TCCVVAASSH QISIFNCDPF GKCFEIDTKN SKKKTSNNNG
60 70 80 90 100
SASNSESRNN EESILITNGS RDRTDAEEEE DNEDNALVTG NILKEGEFVI
110 120 130 140 150
EMLFSTSLIA IADRGQGLNK GKKLKIVNTK RKCTICEIVF PHEIVDVVMN
160 170 180 190 200
RKRMCVLLES DQIFIYDISC MKPLETIDLW EDHYKRSQAN SFSNASNTGT
210 220 230 240 250
LEGDSANLNR VATNLLANAT QKSVNGSNPS VRTRRNSLRS KIRPRMVLSN
260 270 280 290 300
DDRSILCFTA YSSPKKNKPN SEALYDVVIY DTLNVTPVNY LNSVHKGNVA
310 320 330 340 350
CLAVSHDGKL LATASDKGTI IRVFHTGVDS DYMSSRSLFK EFRRGTRLCN
360 370 380 390 400
LYQLAFDKSM TMIGCVGDTD TIHLFKLDDA SNSLPGDNSS NGHWNEEEYI
410 420 430 440 450
LASNSNPSMG TPKEIPLSKP RIANYFSKKI KSSIPNQNLS RNFAYITVNE
460 470 480 490
SNRSCLGFPD EFPNQVYIAS DDGTFSIYSI PSKPGECVLT KNNKFT
Length:496
Mass (Da):55,188
Last modified:November 1, 1996 - v1
Checksum:i4454C28180E3974D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43281 Genomic DNA. Translation: AAB68199.1.
BK006949 Genomic DNA. Translation: DAA11332.1.
PIRiS61966.
RefSeqiNP_015225.1. NM_001183914.1.

Genome annotation databases

EnsemblFungiiYPL100W; YPL100W; YPL100W.
GeneIDi856004.
KEGGisce:YPL100W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43281 Genomic DNA. Translation: AAB68199.1.
BK006949 Genomic DNA. Translation: DAA11332.1.
PIRiS61966.
RefSeqiNP_015225.1. NM_001183914.1.

3D structure databases

ProteinModelPortaliQ02887.
SMRiQ02887. Positions 351-377.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36080. 90 interactions.
IntActiQ02887. 3 interactions.
MINTiMINT-4504851.

PTM databases

iPTMnetiQ02887.

Proteomic databases

MaxQBiQ02887.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL100W; YPL100W; YPL100W.
GeneIDi856004.
KEGGisce:YPL100W.

Organism-specific databases

EuPathDBiFungiDB:YPL100W.
SGDiS000006021. ATG21.

Phylogenomic databases

HOGENOMiHOG000217543.
InParanoidiQ02887.
OMAiPFGKCFE.
OrthoDBiEOG092C3UKJ.

Enzyme and pathway databases

BioCyciYEAST:G3O-34003-MONOMER.

Miscellaneous databases

PROiQ02887.

Family and domain databases

Gene3Di2.130.10.10. 5 hits.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
SMARTiSM00320. WD40. 3 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiATG21_YEAST
AccessioniPrimary (citable) accession number: Q02887
Secondary accession number(s): D6W3R6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6020 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.