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Protein

Elongator complex protein 4

Gene

ELP4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as component of the RNA polymerase II elongator complex, which is a major histone acetyltransferase component of the RNA polymerase II (RNAPII) holoenzyme and is involved in transcriptional elongation. Association with elongating RNAPII requires a hyperphosphorylated state of the RNAPII C-terminal domain (CTD). Elongator binds to both naked and nucleosomal DNA, can acetylate both core and nucleosomal histones, and is involved in chromatin remodeling. It acetylates histones H3, preferentially at 'Lys-14', and H4, preferentially at 'Lys-8'. It functions as a gamma-toxin target (TOT); disruption of the complex confers resistance to Kluyveromyces lactis toxin zymocin (pGKL1 killer toxin). May also be involved in sensitiviy to Pichia inositovora toxin. May be involved in tRNA modification. ELP4 is required for the complex integrity and the complex HAT activity but is not required for the association of the complex with nascent RNA transcript. Is required for an early step in synthesis of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) groups present on uridines at the wobble position in tRNA.7 Publications

Miscellaneous

Present with 358 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • RNA polymerase II core binding Source: GO_Central

GO - Biological processi

  • histone H3 acetylation Source: GO_Central
  • histone H4 acetylation Source: GO_Central
  • regulation of transcription by RNA polymerase II Source: SGD
  • transcription elongation from RNA polymerase II promoter Source: GO_Central
  • tRNA wobble uridine modification Source: SGD

Keywordsi

Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-34004-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Elongator complex protein 4
Alternative name(s):
Gamma-toxin target 7
HAT-associated protein 1
Gene namesi
Name:ELP4
Synonyms:HAP1, TOT7
Ordered Locus Names:YPL101W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi

Organism-specific databases

EuPathDBiFungiDB:YPL101W
SGDiS000006022 ELP4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002358121 – 456Elongator complex protein 4Add BLAST456

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei222PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ02884
PaxDbiQ02884
PRIDEiQ02884

PTM databases

iPTMnetiQ02884

Interactioni

Subunit structurei

Component of the RNA polymerase II elongator complex, which consists of IKI3, ELP2, ELP3, ELP4, IKI1 and ELP6. ELP4, IKI1 and ELP6 form a distinct subcomplex (HAP). ELP4 interacts with KTI12.4 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • RNA polymerase II core binding Source: GO_Central

Protein-protein interaction databases

BioGridi36079, 412 interactors
DIPiDIP-6718N
IntActiQ02884, 7 interactors
MINTiQ02884
STRINGi4932.YPL101W

Structurei

Secondary structure

1456
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi75 – 77Combined sources3
Turni79 – 81Combined sources3
Beta strandi84 – 86Combined sources3
Helixi91 – 96Combined sources6
Beta strandi99 – 103Combined sources5
Beta strandi107 – 112Combined sources6
Helixi119 – 135Combined sources17
Beta strandi149 – 154Combined sources6
Helixi158 – 163Combined sources6
Beta strandi165 – 167Combined sources3
Beta strandi241 – 246Combined sources6
Turni253 – 255Combined sources3
Beta strandi256 – 259Combined sources4
Beta strandi261 – 263Combined sources3
Helixi265 – 278Combined sources14
Turni279 – 281Combined sources3
Beta strandi282 – 288Combined sources7
Turni289 – 292Combined sources4
Turni294 – 296Combined sources3
Helixi299 – 302Combined sources4
Helixi304 – 320Combined sources17
Turni321 – 324Combined sources4
Beta strandi325 – 332Combined sources8
Helixi333 – 335Combined sources3
Helixi338 – 347Combined sources10
Beta strandi349 – 356Combined sources8
Helixi359 – 368Combined sources10
Turni369 – 371Combined sources3
Helixi373 – 375Combined sources3
Beta strandi379 – 385Combined sources7
Helixi389 – 392Combined sources4
Beta strandi398 – 406Combined sources9
Beta strandi411 – 415Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4A8JX-ray2.10A/D66-426[»]
4EJSX-ray2.61A67-438[»]
ProteinModelPortaliQ02884
SMRiQ02884
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ELP4 family.Curated

Phylogenomic databases

HOGENOMiHOG000175573
InParanoidiQ02884
KOiK11375
OMAiERMKIAW
OrthoDBiEOG092C4EY0

Family and domain databases

InterProiView protein in InterPro
IPR008728 Elongator_complex_protein_4
PANTHERiPTHR12896 PTHR12896, 1 hit
PfamiView protein in Pfam
PF05625 PAXNEB, 1 hit

Sequencei

Sequence statusi: Complete.

Q02884-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFRKRGEIL NDRGSGLRGP LLRGPPRTSS TPLRTGNRRA PGNVPLSDTT
60 70 80 90 100
ARLKKLNIAD ESKTKMGLDS SHVGVRPSPA TSQPTTSTGS ADLDSILGHM
110 120 130 140 150
GLPLGNSVLV EEQSTTEFHS ILGKLFAAQG IVHNRISDSS ADKTRNGDTH
160 170 180 190 200
VIVLSLNQMF AKELPGIYKG SRKQMKKNLI SEEESKVTVQ NLNETQRSTP
210 220 230 240 250
SRYKDLKIAW KYKLADEKRL GSPDRDDIQQ NSEYKDYNHQ FDITTRLMPA
260 270 280 290 300
PIASELTFIA PTQPVSTILS QIEQTIKRND KKLIRIVIPS LLHPAMYPPK
310 320 330 340 350
MFESSEIIGL MHGVRSLVKK YYERVVLFAS ISIDIITPPL LVLLRNMFDS
360 370 380 390 400
VINLEPFNQE MTEFLERVYK SQPGKIQHGL VHILKLPVFT DRGEMRVLKS
410 420 430 440 450
EWAFKNGRKK FEIEQWGIPV DDAEGSAASE QSHSHSHSDE ISHNIPAKKT

KISLDY
Length:456
Mass (Da):51,156
Last modified:November 1, 1996 - v1
Checksum:i276F0D1538EAA8FA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43281 Genomic DNA Translation: AAB68198.1
BK006949 Genomic DNA Translation: DAA11331.1
PIRiS61965
RefSeqiNP_015224.1, NM_001183915.1

Genome annotation databases

EnsemblFungiiYPL101W; YPL101W; YPL101W
GeneIDi856002
KEGGisce:YPL101W

Entry informationi

Entry nameiELP4_YEAST
AccessioniPrimary (citable) accession number: Q02884
Secondary accession number(s): D6W3R5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

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