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Protein

N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D, mitochondrial

Gene

FMP30

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce N-acylethanolamines (NAEs).1 Publication

Miscellaneous

Present with 178 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

An N-acylphosphatidylethanolamine + H2O = an N-acylethanolamine + a 1,2-diacylglycerol 3-phosphate.

Cofactori

Zn2+By similarityNote: Binds 1 or 2 zinc ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi265Zinc 1Sequence analysis1
Metal bindingi267Zinc 1Sequence analysis1
Metal bindingi269Zinc 2Sequence analysis1
Metal bindingi270Zinc 2Sequence analysis1
Metal bindingi332Zinc 1Sequence analysis1
Metal bindingi425Zinc 2Sequence analysis1

GO - Molecular functioni

GO - Biological processi

  • N-acylethanolamine metabolic process Source: SGD
  • N-acylphosphatidylethanolamine metabolic process Source: SGD
  • phospholipid catabolic process Source: UniProtKB-KW

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism, Phospholipid degradation, Phospholipid metabolism
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-34005-MONOMER.

Chemistry databases

SwissLipidsiSLP:000000081.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D, mitochondrial (EC:3.1.4.54)
Short name:
NAPE-PLD
Short name:
NAPE-hydrolyzing phospholipase D
Alternative name(s):
Found in mitochondrial proteome protein 30
Gene namesi
Name:FMP30
Ordered Locus Names:YPL103C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL103C.
SGDiS000006024. FMP30.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei54 – 76HelicalSequence analysisAdd BLAST23

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 39MitochondrionSequence analysisAdd BLAST39
ChainiPRO_000023863340 – 468N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D, mitochondrialAdd BLAST429

Proteomic databases

MaxQBiQ02883.
PRIDEiQ02883.

Expressioni

Inductioni

Expressed periodically during the glycolytic and respiratory oscillations cycles.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi36078. 135 interactors.
STRINGi4932.YPL103C.

Structurei

3D structure databases

ProteinModelPortaliQ02883.
SMRiQ02883.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAPE-PLD family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000017990.
HOGENOMiHOG000246526.
InParanoidiQ02883.
OMAiSWHQKPR.
OrthoDBiEOG092C2RGI.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
InterProiView protein in InterPro
IPR001279. Metallo-B-lactamas.
IPR036866. Metallo-hydrolase/OxRdtase.
IPR024884. NAPE-PLD.
PfamiView protein in Pfam
PF12706. Lactamase_B_2. 1 hit.
PIRSFiPIRSF038896. NAPE-PLD. 1 hit.
SUPFAMiSSF56281. SSF56281. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02883-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNFVTCHVQM RLLLQRRLVR LRESELFRPQ TSLSTFKRHA SQKTRPIQKC
60 70 80 90 100
SRKYARILLL SVLVPYTGYA FYVSLATVKQ IDLRNEMCQR LEENNNEVTY
110 120 130 140 150
KGSLLKYSPL EVLGRFENPF EEYRIQTVFE FFANRVFELF ERNRGGIPRD
160 170 180 190 200
VHQMNKLMPV HKPTWGPNLV DVDPAEETAL PLECKVLDEL HIPTAVEENE
210 220 230 240 250
GSKCPVYNTW LGQSCNYTVY NGLRILTDPL FSDFLIHKTL GPKRITQMPS
260 270 280 290 300
QITEVPKPDI ILVSHNHPDH LDLESLEYWS GKDSPLWIVP KGMKSYMTSN
310 320 330 340 350
GCDNVLELSW WETLQVKKNN EIYHISATPA MHWSGRSLLD TNKSLWCSFL
360 370 380 390 400
LTHHGNPILF HAGDTGYVKD LFVRIKERFG KGCKLALLPC GQYCPEWHQK
410 420 430 440 450
PRHINPQEVL KIMKDLEARN VLGVHWGTFV LSGEYFLEPK EKLEMLAEWG
460
GFKDRCYCPE LGKTECFD
Length:468
Mass (Da):54,368
Last modified:November 1, 1996 - v1
Checksum:iC5E6D8817C16791A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43281 Genomic DNA. Translation: AAB68197.1.
BK006949 Genomic DNA. Translation: DAA11330.1.
PIRiS61964.
RefSeqiNP_015222.1. NM_001183917.1.

Genome annotation databases

EnsemblFungiiYPL103C; YPL103C; YPL103C.
GeneIDi856001.
KEGGisce:YPL103C.

Similar proteinsi

Entry informationi

Entry nameiFMP30_YEAST
AccessioniPrimary (citable) accession number: Q02883
Secondary accession number(s): D6W3R4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: November 1, 1996
Last modified: November 22, 2017
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names