Q02866 (MUK1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein MUK1

Gene names

Name:	MUK1
Ordered Locus Names:	YPL070W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	612 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Putative GTPase-activating protein.
Subcellular location	Cytoplasm Ref.3.
Sequence similarities	Contains 1 VPS9 domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from direct assay Ref.3. Source: SGD
Molecular function	GTPase activator activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GCD7	P32502	3	EBI-32646,EBI-6260

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 612	612	Protein MUK1		PRO_0000255969
Regions
	Domain	273 – 414	142	VPS9
	Compositional bias	115 – 195	81	Ser-rich
	Compositional bias	512 – 554	43	Ser-rich
Amino acid modifications
	Modified residue	67	1	Phosphoserine Ref.6
	Modified residue	163	1	Phosphoserine Ref.6
	Modified residue	184	1	Phosphoserine Ref.5 Ref.6
	Modified residue	185	1	Phosphoserine Ref.4 Ref.5 Ref.6
	Modified residue	244	1	Phosphoserine Ref.6
	Modified residue	245	1	Phosphoserine Ref.6
	Modified residue	475	1	Phosphoserine Ref.6
	Modified residue	522	1	Phosphoserine Ref.6
	Modified residue	523	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q02866 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C743D0436E541978	FASTA	612	69,538
	10 20 30 40 50 60 MARQLFTPPI TNPRFDPNQS IRESYKNTTG GMQFQQNLHE DQNDNERSSC DGDENSTTGE 70 80 90 100 110 120 RLENNKSPIL TKQEIDEALN TVTNLPPELS KLIDIFIDDL KQPKYVRPLS VLQLSSLFQS 130 140 150 160 170 180 FYIKFDKASF QHVSSANNNG YYFSGGGSSS FLAAKETLSS GLSGIFGRSR SSSGNSLMRP 190 200 210 220 230 240 RRSSSLFSNE SISNSTNATQ MLSPEEIKKQ LKINELNNMK IEKYMELCER DVFKKILIVG 250 260 270 280 290 300 TSVSSPNKMK TFKPHQLQTF KVGNLFRNSV EFTEYNKLLN EKILCLSKLS TMNKINLIKF 310 320 330 340 350 360 LSLNNGIDPE PKFEEIKDIL YEFTYHSISP CEKIKALLKL HEIMTYSQEM SNDDYLSLLI 370 380 390 400 410 420 YYIITIVPRD IFLNAEFIRL FRYKKKLVET ESFALTNLEA ALVFVEGLTK NDFSNELQDK 430 440 450 460 470 480 LTVNESKILE NSISSRVSLP SKTAIMHKNN GNNGSNLGDI VTPTIQRPDV TRSNSYDGFR 490 500 510 520 530 540 TVFDSSLKNI IGKIRSYTPP HPNNTSNNNL HSSNNLNIPR SSSQLSMELS NRDTTEMSRD 550 560 570 580 590 600 GSRSTSSSSR SSASLEHGNR EFTGDLTVTA SINGADKKEF QKSWKKYKGY KFEDLTICEL 610 RDLFEIYQKM MQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-185, MASS SPECTROMETRY. Strain: ADR376.
[5]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-184 AND SER-185, MASS SPECTROMETRY.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-67; SER-163; SER-184; SER-185; SER-244; SER-245; SER-475; SER-522 AND SER-523, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U41849 Genomic DNA. Translation: AAB68267.1. BK006949 Genomic DNA. Translation: DAA11361.1.
PIR	S61116.
RefSeq	NP_015255.1. NM_001183884.1.
3D structure databases
ProteinModelPortal	Q02866.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1326N.
IntAct	Q02866. 32 interactions.
MINT	MINT-401237.
STRING	Q02866.
Proteomic databases
PeptideAtlas	Q02866.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL070W; YPL070W; YPL070W.
GeneID	856035.
KEGG	sce:YPL070W.
NMPDR	fig|4932.3.peg.6388.
Organism-specific databases
CYGD	YPL070w.
SGD	S000005991. MUK1.
Phylogenomic databases
eggNOG	fuNOG08437.
HOGENOM	HBG203910.
OMA	DDLKQPK.
OrthoDB	EOG4FV07T.
Gene expression databases
ArrayExpress	Q02866.
Genevestigator	Q02866.
GermOnline	YPL070W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003123. VPS9. IPR013995. VPS9_subgr. [Graphical view]
Pfam	PF02204. VPS9. 1 hit. [Graphical view]
SMART	SM00167. VPS9. 1 hit. [Graphical view]
PROSITE	PS51205. VPS9. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980965.

Entry information

Entry name

MUK1_YEAST

Accession

Primary (citable) accession number: Q02866
Secondary accession number(s): D6W3U5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program