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Protein

Ubiquitin carboxyl-terminal hydrolase 16

Gene

UBP16

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei62NucleophilePROSITE-ProRule annotation1
Active sitei407Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

  • thiol-dependent ubiquitin-specific protease activity Source: SGD

GO - Biological processi

  • protein deubiquitination Source: SGD
  • ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-33980-MONOMER.

Protein family/group databases

MEROPSiC19.A18.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 16 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 16
Ubiquitin thioesterase 16
Ubiquitin-specific-processing protease 16
Gene namesi
Name:UBP16
Ordered Locus Names:YPL072W
ORF Names:LPF12W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL072W.
SGDiS000005993. UBP16.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial outer membrane Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000806011 – 499Ubiquitin carboxyl-terminal hydrolase 16Add BLAST499

Proteomic databases

MaxQBiQ02863.
PRIDEiQ02863.

Interactioni

Protein-protein interaction databases

BioGridi36107. 14 interactors.
MINTiMINT-4504680.

Structurei

3D structure databases

ProteinModelPortaliQ02863.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini53 – 497USPAdd BLAST445

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

InParanoidiQ02863.
KOiK11874.
OMAiYEATHAW.
OrthoDBiEOG092C4KM6.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q02863-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWIKNVTES PTSLIKKVSC GLIIAASLYA IAPSLSALVF GDSKQSIGKY
60 70 80 90 100
TTVGLINRGN DCFITSSLQG LAGIPRFVEY LKRIRTVLLE LETKLSNNAK
110 120 130 140 150
GDNPTVDNTT RHSRLENSSN SLAPLHESLT SLILDLISVK DRKTSISPKI
160 170 180 190 200
VINTLESIFK SKISSKQNDA HEFTLILLQT LQEERSKLID YSKQICNLNI
210 220 230 240 250
PKFPFEGETS KFLVCLKCKG LSEPSYKQTF IRELSVPQQT SENLSNILAH
260 270 280 290 300
DETEIIDDYS CLICQIRAIL NHEEYRNFKD CTPDEILMLD RLKNYATKAP
310 320 330 340 350
INENLPFEVE QYVKRYSKGN LQVSNIKGKV IKKDVVVQLP DILIVHLSRS
360 370 380 390 400
TFNGITYSRN PCNVKFGERI TLSEYTLAES GTITENRQVK YNLKSVVKHT
410 420 430 440 450
GSHSSGHYMC YRRKTEIRFG KEDESSFRRA PVVNNEVNKN REQNVAHNDY
460 470 480 490
KKSRYKKVKN ALRYPYWQIS DTAIKESTAS TVLNEQKYAY MLYYERVNK
Length:499
Mass (Da):56,920
Last modified:November 1, 1996 - v1
Checksum:i292021C9A268B4D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41849 Genomic DNA. Translation: AAB68265.1.
BK006949 Genomic DNA. Translation: DAA11359.1.
PIRiS61114.
RefSeqiNP_015253.1. NM_001183886.1.

Genome annotation databases

EnsemblFungiiYPL072W; YPL072W; YPL072W.
GeneIDi856032.
KEGGisce:YPL072W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41849 Genomic DNA. Translation: AAB68265.1.
BK006949 Genomic DNA. Translation: DAA11359.1.
PIRiS61114.
RefSeqiNP_015253.1. NM_001183886.1.

3D structure databases

ProteinModelPortaliQ02863.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36107. 14 interactors.
MINTiMINT-4504680.

Protein family/group databases

MEROPSiC19.A18.

Proteomic databases

MaxQBiQ02863.
PRIDEiQ02863.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL072W; YPL072W; YPL072W.
GeneIDi856032.
KEGGisce:YPL072W.

Organism-specific databases

EuPathDBiFungiDB:YPL072W.
SGDiS000005993. UBP16.

Phylogenomic databases

InParanoidiQ02863.
KOiK11874.
OMAiYEATHAW.
OrthoDBiEOG092C4KM6.

Enzyme and pathway databases

BioCyciYEAST:G3O-33980-MONOMER.

Miscellaneous databases

PROiQ02863.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBP16_YEAST
AccessioniPrimary (citable) accession number: Q02863
Secondary accession number(s): D6W3U3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 450 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.