Q02863 (UBP16_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 16
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 16
Ubiquitin thioesterase 16
Ubiquitin-specific-processing protease 16

Gene names

Name:	UBP16
Ordered Locus Names:	YPL072W
ORF Names:	LPF12W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Miscellaneous	Present with 450 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the peptidase C19 family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein deubiquitination Traceable author statement PubMed 8982460. Source: SGD ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	mitochondrial outer membrane Inferred from direct assay PubMed 12914939PubMed 16407407. Source: SGD
Molecular_function	ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro ubiquitin-specific protease activity Traceable author statement PubMed 8982460. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Ubiquitin carboxyl-terminal hydrolase 16		PRO_0000080601
Sites
	Active site	62	1	Nucleophile By similarity
	Active site	407	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q02863 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 292021C9A268B4D1	FASTA	499	56,920
	10 20 30 40 50 60 MSWIKNVTES PTSLIKKVSC GLIIAASLYA IAPSLSALVF GDSKQSIGKY TTVGLINRGN 70 80 90 100 110 120 DCFITSSLQG LAGIPRFVEY LKRIRTVLLE LETKLSNNAK GDNPTVDNTT RHSRLENSSN 130 140 150 160 170 180 SLAPLHESLT SLILDLISVK DRKTSISPKI VINTLESIFK SKISSKQNDA HEFTLILLQT 190 200 210 220 230 240 LQEERSKLID YSKQICNLNI PKFPFEGETS KFLVCLKCKG LSEPSYKQTF IRELSVPQQT 250 260 270 280 290 300 SENLSNILAH DETEIIDDYS CLICQIRAIL NHEEYRNFKD CTPDEILMLD RLKNYATKAP 310 320 330 340 350 360 INENLPFEVE QYVKRYSKGN LQVSNIKGKV IKKDVVVQLP DILIVHLSRS TFNGITYSRN 370 380 390 400 410 420 PCNVKFGERI TLSEYTLAES GTITENRQVK YNLKSVVKHT GSHSSGHYMC YRRKTEIRFG 430 440 450 460 470 480 KEDESSFRRA PVVNNEVNKN REQNVAHNDY KKSRYKKVKN ALRYPYWQIS DTAIKESTAS 490 TVLNEQKYAY MLYYERVNK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U41849 Genomic DNA. Translation: AAB68265.1. BK006949 Genomic DNA. Translation: DAA11359.1.
PIR	S61114.
RefSeq	NP_015253.1. NM_001183886.1.
3D structure databases
ProteinModelPortal	Q02863.
SMR	Q02863. Positions 39-411.
ModBase	Search...
Protein-protein interaction databases
STRING	4932.YPL072W.
Protein family/group databases
MEROPS	C19.A18.
Proteomic databases
PaxDb	Q02863.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL072W; YPL072W; YPL072W.
GeneID	856032.
KEGG	sce:YPL072W.
Organism-specific databases
CYGD	YPL072w.
SGD	S000005993. UBP16.
Phylogenomic databases
eggNOG	NOG40279.
KO	K11874.
OMA	LPDITIN.
OrthoDB	EOG4ZKNW3.
Gene expression databases
Genevestigator	Q02863.
GermOnline	YPL072W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980959.

Entry information

Entry name

UBP16_YEAST

Accession

Primary (citable) accession number: Q02863
Secondary accession number(s): D6W3U3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program