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Q02853

- MMP11_MOUSE

UniProt

Q02853 - MMP11_MOUSE

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Protein

Stromelysin-3

Gene

Mmp11

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May play an important role in the progression of epithelial malignancies.

Cofactori

Protein has several cofactor binding sites:
  • Ca2+Note: Binds 1 Ca(2+) ion per subunit.
  • Zn2+Note: Binds 2 Zn(2+) ions per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi84 – 841Zinc 2; in inhibited formBy similarity
Metal bindingi168 – 1681Zinc 1
Metal bindingi170 – 1701Zinc 1
Metal bindingi175 – 1751Calcium
Metal bindingi176 – 1761Calcium; via carbonyl oxygen
Metal bindingi178 – 1781Calcium; via carbonyl oxygen
Metal bindingi180 – 1801Calcium; via carbonyl oxygen
Metal bindingi183 – 1831Zinc 1
Metal bindingi196 – 1961Zinc 1
Metal bindingi219 – 2191Zinc 2; catalytic
Active sitei220 – 2201
Metal bindingi223 – 2231Zinc 2; catalytic
Metal bindingi229 – 2291Zinc 2; catalytic

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. metalloendopeptidase activity Source: InterPro
  3. metallopeptidase activity Source: MGI
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. basement membrane organization Source: MGI
  2. collagen catabolic process Source: MGI
  3. collagen fibril organization Source: MGI
  4. extracellular matrix organization Source: MGI
  5. multicellular organismal development Source: InterPro
  6. negative regulation of fat cell differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Collagen degradation

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_199000. Activation of Matrix Metalloproteinases.
REACT_199055. Collagen degradation.

Protein family/group databases

MEROPSiM10.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Stromelysin-3 (EC:3.4.24.-)
Short name:
SL-3
Short name:
ST3
Alternative name(s):
Matrix metalloproteinase-11
Short name:
MMP-11
Gene namesi
Name:Mmp11
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:97008. Mmp11.

Subcellular locationi

GO - Cellular componenti

  1. extracellular matrix Source: MGI
  2. proteinaceous extracellular matrix Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3535Sequence AnalysisAdd
BLAST
Propeptidei36 – 10166Activation peptideBy similarityPRO_0000028772Add
BLAST
Chaini102 – 492391Stromelysin-3PRO_0000028773Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi298 ↔ 484By similarity

Post-translational modificationi

The precursor is cleaved by a furin endopeptidase.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Zymogen

Proteomic databases

MaxQBiQ02853.
PRIDEiQ02853.

PTM databases

PhosphoSiteiQ02853.

Miscellaneous databases

PMAP-CutDBQ02853.

Expressioni

Tissue specificityi

Specifically expressed in the mammary gland during apoptosis.

Gene expression databases

BgeeiQ02853.
CleanExiMM_MMP11.
ExpressionAtlasiQ02853. baseline and differential.
GenevestigatoriQ02853.

Interactioni

Structurei

Secondary structure

1
492
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi102 – 1054Combined sources
Beta strandi108 – 1114Combined sources
Beta strandi113 – 1186Combined sources
Helixi127 – 14216Combined sources
Beta strandi148 – 1514Combined sources
Beta strandi153 – 1564Combined sources
Beta strandi158 – 1647Combined sources
Beta strandi168 – 1714Combined sources
Beta strandi176 – 1794Combined sources
Beta strandi182 – 1854Combined sources
Beta strandi190 – 1989Combined sources
Beta strandi203 – 2064Combined sources
Beta strandi208 – 2125Combined sources
Helixi213 – 22412Combined sources
Helixi251 – 26010Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HV5X-ray2.60A/B/C/D/E/F102-265[»]
ProteinModelPortaliQ02853.
SMRiQ02853. Positions 102-463.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ02853.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati295 – 34349Hemopexin 1Add
BLAST
Repeati344 – 38643Hemopexin 2Add
BLAST
Repeati388 – 43649Hemopexin 3Add
BLAST
Repeati437 – 48448Hemopexin 4Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi82 – 898Cysteine switchBy similarity

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Belongs to the peptidase M10A family.Curated
Contains 4 hemopexin repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG236137.
HOGENOMiHOG000217927.
HOVERGENiHBG052484.
InParanoidiQ02853.
KOiK07993.
OMAiVDTNEIA.
OrthoDBiEOG7XPZ57.
PhylomeDBiQ02853.
TreeFamiTF315428.

Family and domain databases

Gene3Di2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR018486. Hemopexin_CS.
IPR024079. MetalloPept_cat_dom.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_stromelysin-type.
IPR006026. Peptidase_Metallo.
IPR028705. Stromelysin-3.
[Graphical view]
PANTHERiPTHR10201:SF20. PTHR10201:SF20. 1 hit.
PfamiPF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
[Graphical view]
PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSiPR00138. MATRIXIN.
SMARTiSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF50923. SSF50923. 1 hit.
PROSITEiPS00024. HEMOPEXIN. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02853-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MARAACLLRA ISRVLLLPLP LLLLLLLLLP SPLMARARPP ESHRHHPVKK
60 70 80 90 100
GPRLLHAALP NTLTSVPASH WVPSPAGSSR PLRCGVPDLP DVLNARNRQK
110 120 130 140 150
RFVLSGGRWE KTDLTYRILR FPWQLVREQV RQTVAEALQV WSEVTPLTFT
160 170 180 190 200
EVHEGRADIM IDFARYWHGD NLPFDGPGGI LAHAFFPKTH REGDVHFDYD
210 220 230 240 250
ETWTIGDNQG TDLLQVAAHE FGHVLGLQHT TAAKALMSPF YTFRYPLSLS
260 270 280 290 300
PDDRRGIQHL YGRPQMAPTS PAPTLSSQAG TDTNEIALLE PETPPDVCET
310 320 330 340 350
SFDAVSTIRG ELFFFKAGFV WRLRSGRLQP GYPALASRHW QGLPSPVDAA
360 370 380 390 400
FEDAQGQIWF FQGAQYWVYD GEKPVLGPAP LSKLGLQGSP VHAALVWGPE
410 420 430 440 450
KNKIYFFRGG DYWRFHPRTQ RVDNPVPRRS TDWRGVPSEI DAAFQDAEGY
460 470 480 490
AYFLRGHLYW KFDPVKVKVL EGFPRPVGPD FFDCAEPANT FR
Length:492
Mass (Da):55,441
Last modified:February 1, 1996 - v2
Checksum:iB54E260E4AB3D7C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z12604 mRNA. Translation: CAA78248.1.
CCDSiCCDS23937.1.
PIRiA44399.
RefSeqiNP_032632.1. NM_008606.2.
XP_006513381.1. XM_006513318.1.
UniGeneiMm.4561.

Genome annotation databases

EnsembliENSMUST00000000924; ENSMUSP00000000924; ENSMUSG00000000901.
ENSMUST00000120281; ENSMUSP00000112940; ENSMUSG00000000901.
GeneIDi17385.
KEGGimmu:17385.
UCSCiuc007fto.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z12604 mRNA. Translation: CAA78248.1 .
CCDSi CCDS23937.1.
PIRi A44399.
RefSeqi NP_032632.1. NM_008606.2.
XP_006513381.1. XM_006513318.1.
UniGenei Mm.4561.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1HV5 X-ray 2.60 A/B/C/D/E/F 102-265 [» ]
ProteinModelPortali Q02853.
SMRi Q02853. Positions 102-463.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi Q02853.
ChEMBLi CHEMBL3412.

Protein family/group databases

MEROPSi M10.007.

PTM databases

PhosphoSitei Q02853.

Proteomic databases

MaxQBi Q02853.
PRIDEi Q02853.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000000924 ; ENSMUSP00000000924 ; ENSMUSG00000000901 .
ENSMUST00000120281 ; ENSMUSP00000112940 ; ENSMUSG00000000901 .
GeneIDi 17385.
KEGGi mmu:17385.
UCSCi uc007fto.1. mouse.

Organism-specific databases

CTDi 4320.
MGIi MGI:97008. Mmp11.

Phylogenomic databases

eggNOGi NOG236137.
HOGENOMi HOG000217927.
HOVERGENi HBG052484.
InParanoidi Q02853.
KOi K07993.
OMAi VDTNEIA.
OrthoDBi EOG7XPZ57.
PhylomeDBi Q02853.
TreeFami TF315428.

Enzyme and pathway databases

Reactomei REACT_199000. Activation of Matrix Metalloproteinases.
REACT_199055. Collagen degradation.

Miscellaneous databases

EvolutionaryTracei Q02853.
NextBioi 291992.
PMAP-CutDB Q02853.
PROi Q02853.
SOURCEi Search...

Gene expression databases

Bgeei Q02853.
CleanExi MM_MMP11.
ExpressionAtlasi Q02853. baseline and differential.
Genevestigatori Q02853.

Family and domain databases

Gene3Di 2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProi IPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR018486. Hemopexin_CS.
IPR024079. MetalloPept_cat_dom.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_stromelysin-type.
IPR006026. Peptidase_Metallo.
IPR028705. Stromelysin-3.
[Graphical view ]
PANTHERi PTHR10201:SF20. PTHR10201:SF20. 1 hit.
Pfami PF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
[Graphical view ]
PIRSFi PIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSi PR00138. MATRIXIN.
SMARTi SM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view ]
SUPFAMi SSF50923. SSF50923. 1 hit.
PROSITEi PS00024. HEMOPEXIN. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The breast cancer-associated stromelysin-3 gene is expressed during mouse mammary gland apoptosis."
    Lefebvre O., Wolf C., Limacher J.-M., Hutin P., Wendling C., Lemeur M., Basset P., Rio M.C.
    J. Cell Biol. 119:997-1002(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Lefebvre O.
    Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "Crystal structure of the stromelysin-3 (MMP-11) catalytic domain complexed with a phosphinic inhibitor mimicking the transition-state."
    Gall A.-L., Ruff M., Kannan R., Cuniasse P., Yiotakis A., Dive V., Rio M.-C., Basset P., Moras D.
    J. Mol. Biol. 307:577-586(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 102-265.

Entry informationi

Entry nameiMMP11_MOUSE
AccessioniPrimary (citable) accession number: Q02853
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: February 1, 1996
Last modified: November 26, 2014
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3