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Reviewed, UniProtKB/Swiss-Prot Q02853 (MMP11_MOUSE)

Last modified January 19, 2010. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Stromelysin-3
      Short name=SL-3
      Short name=ST3
    EC=3.4.24.-
Alternative name(s):
    Matrix metalloproteinase-11
      Short name=MMP-11
Gene names
Name: Mmp11
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length492 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

May play an important role in the progression of epithelial malignancies.

Cofactor

Binds 1 calcium ion per subunit.

Binds 2 zinc ions per subunit.

Subcellular location

Secreted › extracellular space › extracellular matrix Probable.

Tissue specificity

Specifically expressed in the mammary gland during apoptosis.

Domain

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Post-translational modification

The precursor is cleaved by a furin endopeptidase By similarity.

Sequence similarities

Belongs to the peptidase M10A family.

Contains 4 hemopexin-like domains.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3535 Potential
Propeptide36 – 10166Activation peptide By similarity
PRO_0000028772
Chain102 – 492391Stromelysin-3
PRO_0000028773

Regions

Domain302 – 34544Hemopexin-like 1
Domain347 – 38842Hemopexin-like 2
Domain391 – 43848Hemopexin-like 3
Domain440 – 48445Hemopexin-like 4
Motif82 – 898Cysteine switch By similarity

Sites

Active site2201
Metal binding841Zinc 2; in inhibited form By similarity
Metal binding1681Zinc 1
Metal binding1701Zinc 1
Metal binding1751Calcium
Metal binding1761Calcium; via carbonyl oxygen
Metal binding1781Calcium; via carbonyl oxygen
Metal binding1801Calcium; via carbonyl oxygen
Metal binding1831Zinc 1
Metal binding1961Zinc 1
Metal binding2191Zinc 2; catalytic
Metal binding2231Zinc 2; catalytic
Metal binding2291Zinc 2; catalytic

Amino acid modifications

Disulfide bond298 ↔ 484 By similarity

Secondary structure

1 .............................. 492
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
Q02853-1 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: B54E260E4AB3D7C3

FASTA49255,441
        10         20         30         40         50         60 
MARAACLLRA ISRVLLLPLP LLLLLLLLLP SPLMARARPP ESHRHHPVKK GPRLLHAALP 

        70         80         90        100        110        120 
NTLTSVPASH WVPSPAGSSR PLRCGVPDLP DVLNARNRQK RFVLSGGRWE KTDLTYRILR 

       130        140        150        160        170        180 
FPWQLVREQV RQTVAEALQV WSEVTPLTFT EVHEGRADIM IDFARYWHGD NLPFDGPGGI 

       190        200        210        220        230        240 
LAHAFFPKTH REGDVHFDYD ETWTIGDNQG TDLLQVAAHE FGHVLGLQHT TAAKALMSPF 

       250        260        270        280        290        300 
YTFRYPLSLS PDDRRGIQHL YGRPQMAPTS PAPTLSSQAG TDTNEIALLE PETPPDVCET 

       310        320        330        340        350        360 
SFDAVSTIRG ELFFFKAGFV WRLRSGRLQP GYPALASRHW QGLPSPVDAA FEDAQGQIWF 

       370        380        390        400        410        420 
FQGAQYWVYD GEKPVLGPAP LSKLGLQGSP VHAALVWGPE KNKIYFFRGG DYWRFHPRTQ 

       430        440        450        460        470        480 
RVDNPVPRRS TDWRGVPSEI DAAFQDAEGY AYFLRGHLYW KFDPVKVKVL EGFPRPVGPD 

       490 
FFDCAEPANT FR

« Hide

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References

[1]"The breast cancer-associated stromelysin-3 gene is expressed during mouse mammary gland apoptosis."
Lefebvre O., Wolf C., Limacher J.-M., Hutin P., Wendling C., Lemeur M., Basset P., Rio M.C.
J. Cell Biol. 119:997-1002(1992) [PubMed: 1429845] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Lefebvre O.
Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[3]"Crystal structure of the stromelysin-3 (MMP-11) catalytic domain complexed with a phosphinic inhibitor mimicking the transition-state."
Gall A.-L., Ruff M., Kannan R., Cuniasse P., Yiotakis A., Dive V., Rio M.-C., Basset P., Moras D.
J. Mol. Biol. 307:577-586(2001) [PubMed: 11254383] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 102-265.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z12604 mRNA. Translation: CAA78248.1.
IPIIPI00132396.
PIRA44399.
RefSeqNP_032632.1.
UniGeneMm.4561

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1HV5X-ray2.60A/B/C/D/E/F102-265[»]
SMRQ02853. Positions 79-484, 296-486.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ02853.

Protein family/group databases

MEROPSM10.007.

PTM databases

PhosphoSiteQ02853.

Proteomic databases

PRIDEQ02853.

Genome annotation databases

EnsemblENSMUST00000000924; ENSMUSP00000000924; ENSMUSG00000000901; Mus musculus. [Genome view]
ENSMUST00000120281; ENSMUSP00000112940; ENSMUSG00000000901; Mus musculus. [Genome view]
GeneID17385.
KEGGmmu:17385.
UCSCuc007fto.1. mouse.

Organism-specific databases

CTD17385.
MGIMGI:97008. Mmp11.

Phylogenomic databases

eggNOGroNOG07116.
HOGENOMHBG747685.
HOVERGENQ02853.
InParanoidQ02853.
OMANRQKRFV.
OrthoDBEOG9TQPW0.
PhylomeDBQ02853.

Gene expression databases

ArrayExpressQ02853.
BgeeQ02853.
CleanExMM_MMP11.
GenevestigatorQ02853.
GermOnlineENSMUSG00000000901. Mus musculus.

Family and domain databases

InterProIPR000585. Hemopexin/matrixin.
IPR018486. Hemopexin/matrixin_CS.
IPR018487. Hemopexin/matrixin_repeat.
IPR001818. Pept_M10A_M12B.
IPR016293. Pept_M10A_matrix.
IPR006026. Peptidase_M.
[Graphical view]
Gene3DG3DSA:2.110.10.10. Hemopexin. 1 hit.
PfamPF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
[Graphical view]
PIRSFPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSPR00138. MATRIXIN.
SMARTSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
PROSITEPS00546. CYSTEINE_SWITCH. False negative.
PS00024. HEMOPEXIN. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio291992.
PMAP-CutDBQ02853.
SOURCESearch...

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Entry information

Entry nameMMP11_MOUSE
AccessionPrimary (citable) accession number: Q02853
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: February 1, 1996
Last modified: January 19, 2010
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents