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Protein

Tryptase

Gene

Tpsab1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity (By similarity).By similarity

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa, but with more restricted specificity than trypsin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei72Charge relay systemBy similarity1
Active sitei119Charge relay systemBy similarity1
Active sitei222Charge relay systemBy similarity1

GO - Molecular functioni

  • peptidase activity Source: MGI
  • serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  • negative regulation of blood coagulation Source: MGI
  • proteolysis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.026.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptase (EC:3.4.21.59)
Alternative name(s):
Mast cell protease 7
Short name:
mMCP-7
Tryptase alpha/beta-1
Gene namesi
Name:Tpsab1
Synonyms:Mcpt7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:96943. Tpsab1.

Subcellular locationi

GO - Cellular componenti

  • extracellular space Source: MGI
  • mast cell granule Source: MGI
Complete GO annotation...

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4749.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
PropeptideiPRO_000002749419 – 28Activation peptideSequence analysis10
ChainiPRO_000002749529 – 273TryptaseAdd BLAST245

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi49N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi57 ↔ 73PROSITE-ProRule annotation
Glycosylationi130N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi153 ↔ 228PROSITE-ProRule annotation
Disulfide bondi186 ↔ 209PROSITE-ProRule annotation
Disulfide bondi218 ↔ 246PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ02844.
PRIDEiQ02844.

PTM databases

PhosphoSitePlusiQ02844.

Expressioni

Developmental stagei

Is not expressed in mature serosal or mucosal mast cells and is expressed only transiently at an early stage of in vitro mast cell differentiation.

Gene expression databases

CleanExiMM_TPSAB1.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000120741.

Chemistry databases

BindingDBiQ02844.

Structurei

3D structure databases

ProteinModelPortaliQ02844.
SMRiQ02844.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 270Peptidase S1PROSITE-ProRule annotationAdd BLAST242

Sequence similaritiesi

Belongs to the peptidase S1 family. Tryptase subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ02844.
KOiK01340.
PhylomeDBiQ02844.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: The alternative splicing event is due to a G to A point mutation at the exon 2/intron 2 splice site and causes loss of protein expression. The alternatively spliced transcript is only found in C57BL/6 mouse.
Isoform 1 (identifier: Q02844-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLKLLLLTLP LLSSLVHAAP GPAMTREGIV GGQEAHGNKW PWQVSLRAND
60 70 80 90 100
TYWMHFCGGS LIHPQWVLTA AHCVGPDVAD PNKVRVQLRK QYLYYHDHLM
110 120 130 140 150
TVSQIITHPD FYIVQDGADI ALLKLTNPVN ISDYVHPVPL PPASETFPSG
160 170 180 190 200
TLCWVTGWGN IDNGVNLPPP FPLKEVQVPI IENHLCDLKY HKGLITGDNV
210 220 230 240 250
HIVRDDMLCA GNEGHDSCQG DSGGPLVCKV EDTWLQAGVV SWGEGCAQPN
260 270
RPGIYTRVTY YLDWIHHYVP KDF
Length:273
Mass (Da):30,337
Last modified:July 1, 1993 - v1
Checksum:i50ECB4765294205E
GO
Isoform 2 (identifier: Q02844-2) [UniParc]FASTAAdd to basket
Also known as: Truncated

The sequence of this isoform differs from the canonical sequence as follows:
     44-46: VSL → GCC
     47-273: Missing.

Show »
Length:46
Mass (Da):4,896
Checksum:iD91205D8F7A50D75
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00537844 – 46VSL → GCC in isoform 2. 1 Publication3
Alternative sequenceiVSP_00537947 – 273Missing in isoform 2. 1 PublicationAdd BLAST227

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00653 mRNA. Translation: AAA39992.1.
L00654 Genomic DNA. Translation: AAA39993.1.
U42405 mRNA. Translation: AAA97874.1.
U42406 Genomic DNA. Translation: AAA97875.1.
PIRiA47246.
RefSeqiNP_112464.4. NM_031187.4. [Q02844-1]
UniGeneiMm.3301.

Genome annotation databases

GeneIDi100503895.
KEGGimmu:100503895.
UCSCiuc008baq.2. mouse. [Q02844-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00653 mRNA. Translation: AAA39992.1.
L00654 Genomic DNA. Translation: AAA39993.1.
U42405 mRNA. Translation: AAA97874.1.
U42406 Genomic DNA. Translation: AAA97875.1.
PIRiA47246.
RefSeqiNP_112464.4. NM_031187.4. [Q02844-1]
UniGeneiMm.3301.

3D structure databases

ProteinModelPortaliQ02844.
SMRiQ02844.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000120741.

Chemistry databases

BindingDBiQ02844.
ChEMBLiCHEMBL4749.

Protein family/group databases

MEROPSiS01.026.

PTM databases

PhosphoSitePlusiQ02844.

Proteomic databases

PaxDbiQ02844.
PRIDEiQ02844.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100503895.
KEGGimmu:100503895.
UCSCiuc008baq.2. mouse. [Q02844-1]

Organism-specific databases

CTDi7177.
MGIiMGI:96943. Tpsab1.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ02844.
KOiK01340.
PhylomeDBiQ02844.

Miscellaneous databases

PROiQ02844.
SOURCEiSearch...

Gene expression databases

CleanExiMM_TPSAB1.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRYB1_MOUSE
AccessioniPrimary (citable) accession number: Q02844
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 30, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.