Reviewed, UniProtKB/Swiss-Prot Q02844 (TRYB1_MOUSE)

Names and origin

Protein names

Recommended name:
    Tryptase
    EC=3.4.21.59
Alternative name(s):
    Mast cell protease 7
    MMCP-7
    Tryptase alpha/beta-1

Gene names

Name:	Tpsab1
Synonyms:	Mcpt7

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.
Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa, but with more restricted specificity than trypsin.
Developmental stage	Is not expressed in mature serosal or mucosal mast cells and is expressed only transiently at an early stage of in vitro mast cell differentiation.
Sequence similarities	Belongs to the peptidase S1 family. Tryptase subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: The alternative splicing event is due to a G to A point mutation at the exon 2/intron 2 splice site and causes loss of protein expression. The alternatively spliced transcript is only found in C57BL/6 mouse.

Isoform 1 (identifier: Q02844-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q02844-2)

Also known as: Truncated;

The sequence of this isoform differs from the canonical sequence as follows:
     44-46: VSL → GCC
     47-273: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Propeptide	19 – 28	10	Activation peptide Potential		PRO_0000027494
	Chain	29 – 273	245	Tryptase		PRO_0000027495
Regions
	Domain	29 – 270	242	Peptidase S1
Sites
	Active site	72	1	Charge relay system By similarity
	Active site	119	1	Charge relay system By similarity
	Active site	222	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	49	1	N-linked (GlcNAc...) Potential
	Glycosylation	130	1	N-linked (GlcNAc...) Potential
	Disulfide bond	57 ↔ 73		By similarity
	Disulfide bond	153 ↔ 228		By similarity
	Disulfide bond	186 ↔ 209		By similarity
	Disulfide bond	218 ↔ 246		By similarity
Natural variations
	Alternative sequence	44 – 46	3	VSL → GCC in isoform 2.		VSP_005378
	Alternative sequence	47 – 273	227	Missing in isoform 2.		VSP_005379

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 50ECB4765294205E	FASTA	273	30,337
	10 20 30 40 50 60 MLKLLLLTLP LLSSLVHAAP GPAMTREGIV GGQEAHGNKW PWQVSLRAND TYWMHFCGGS 70 80 90 100 110 120 LIHPQWVLTA AHCVGPDVAD PNKVRVQLRK QYLYYHDHLM TVSQIITHPD FYIVQDGADI 130 140 150 160 170 180 ALLKLTNPVN ISDYVHPVPL PPASETFPSG TLCWVTGWGN IDNGVNLPPP FPLKEVQVPI 190 200 210 220 230 240 IENHLCDLKY HKGLITGDNV HIVRDDMLCA GNEGHDSCQG DSGGPLVCKV EDTWLQAGVV 250 260 270 SWGEGCAQPN RPGIYTRVTY YLDWIHHYVP KDF
	Isoform 2 (Truncated). Checksum: D91205D8F7A50D75	FASTA	46	4,896
	10 20 30 40 MLKLLLLTLP LLSSLVHAAP GPAMTREGIV GGQEAHGNKW PWQGCC

References

[1]	"Isolation, characterization, and transcription of the gene encoding mouse mast cell protease 7." McNeil H.P., Reynolds D.S., Schiller V., Ghildyal N., Gurley D.S., Austen K.F., Stevens R.L. Proc. Natl. Acad. Sci. U.S.A. 89:11174-11178(1992) [PubMed: 1454796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1). Strain: DBA/2.
[2]	"Natural disruption of the mouse mast cell protease 7 gene in the C57BL/6 mouse." Hunt J.E., Stevens R.L., Austen K.F., Zhang J., Xia Z., Ghildyal N. J. Biol. Chem. 271:2851-2855(1996) [PubMed: 8576265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 2). Strain: C57BL/6J.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L00653 mRNA. Translation: AAA39992.1. L00654 Genomic DNA. Translation: AAA39993.1. U42405 mRNA. Translation: AAA97874.1. U42406 Genomic DNA. Translation: AAA97875.1.
IPI	IPI00222569. IPI00331126.
PIR	A47246.
UniGene	Mm.3301
3D structure databases
HSSP	HSSP built from PDB template 1LTO based on UniProtKB P15157.
SMR	Q02844. Positions 29-271.
ModBase	Search...
Protein family/group databases
MEROPS	S01.026.
Genome annotation databases
Ensembl	ENSMUSG00000024173. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:96943. Tpsab1.
Phylogenomic databases
HOGENOM	Q02844.
HOVERGEN	Q02844.
Enzyme and pathway databases
BRENDA	3.4.21.59. 244.
Gene expression databases
ArrayExpress	Q02844.
Bgee	Q02844.
CleanEx	MM_TPSAB1.
GermOnline	ENSMUSG00000024173. Mus musculus.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

TRYB1_MOUSE

Accession

Primary (citable) accession number: Q02844

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	May 5, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)