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Q02834

- NANH_MICVI

UniProt

Q02834 - NANH_MICVI

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Protein

Sialidase

Gene
nedA
Organism
Micromonospora viridifaciens
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

To release sialic acids for use as carbon and energy sources for this non-pathogenic bacterium while in pathogenic microorganisms, sialidases have been suggested to be pathogenic factors.

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei68 – 681Substrate By similarity
Active sitei92 – 921Proton acceptor By similarity
Active sitei260 – 2601Nucleophile Reviewed prediction
Binding sitei276 – 2761Substrate By similarity
Active sitei370 – 3701Nucleophile

GO - Molecular functioni

  1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB-EC
  2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
  3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell adhesion Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

SABIO-RKQ02834.

Protein family/group databases

CAZyiCBM32. Carbohydrate-Binding Module Family 32.
GH33. Glycoside Hydrolase Family 33.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialidase (EC:3.2.1.18)
Alternative name(s):
Neuraminidase
Gene namesi
Name:nedA
OrganismiMicromonospora viridifaciens
Taxonomic identifieri1881 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicromonosporineaeMicromonosporaceaeMicromonospora

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 37371 PublicationAdd
BLAST
Chaini38 – 647610SialidasePRO_0000012032Add
BLAST

Expressioni

Inductioni

By N-acetylneuraminic acid, colominic acid, and sialic acid.

Structurei

Secondary structure

1
647
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi51 – 588
Beta strandi61 – 633
Beta strandi65 – 7410
Beta strandi80 – 878
Beta strandi98 – 1069
Beta strandi107 – 1093
Beta strandi115 – 1184
Beta strandi123 – 1253
Beta strandi127 – 13610
Turni138 – 1403
Beta strandi143 – 15210
Turni155 – 1573
Beta strandi171 – 1799
Beta strandi185 – 1884
Helixi190 – 1934
Beta strandi201 – 2044
Beta strandi206 – 2083
Turni216 – 2194
Beta strandi221 – 2288
Beta strandi234 – 24310
Beta strandi260 – 2656
Beta strandi271 – 2755
Beta strandi282 – 29110
Beta strandi300 – 3067
Beta strandi313 – 3186
Helixi327 – 3293
Beta strandi331 – 3366
Beta strandi339 – 35214
Beta strandi359 – 3679
Beta strandi371 – 3755
Beta strandi381 – 3855
Beta strandi388 – 3969
Helixi398 – 4014
Beta strandi413 – 4153
Beta strandi420 – 4289
Beta strandi431 – 4333
Beta strandi435 – 4373
Beta strandi439 – 4435
Beta strandi448 – 4547
Beta strandi462 – 4709
Beta strandi478 – 48811
Beta strandi491 – 50313
Helixi506 – 5083
Beta strandi510 – 5156
Beta strandi520 – 5223
Helixi526 – 5305
Beta strandi543 – 5453
Beta strandi552 – 57120
Beta strandi576 – 5783
Beta strandi582 – 59312
Beta strandi595 – 6028
Beta strandi610 – 62617
Beta strandi637 – 6459

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EURX-ray1.82A43-407[»]
1EUSX-ray2.00A43-407[»]
1EUTX-ray2.50A43-647[»]
1EUUX-ray2.50A43-647[»]
1W8NX-ray2.10A47-647[»]
1W8OX-ray1.70A47-647[»]
1WCQX-ray2.10A/B/C47-647[»]
2BERX-ray1.80A47-647[»]
2BZDX-ray2.00A/B/C47-647[»]
4J9TX-ray1.40A47-407[»]
ProteinModelPortaliQ02834.
SMRiQ02834. Positions 47-647.

Miscellaneous databases

EvolutionaryTraceiQ02834.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati102 – 11312BNR 1Add
BLAST
Repeati175 – 18612BNR 2Add
BLAST
Repeati239 – 25012BNR 3Add
BLAST
Repeati287 – 29812BNR 4Add
BLAST
Repeati348 – 35912BNR 5Add
BLAST
Domaini496 – 646151F5/8 type CAdd
BLAST

Sequence similaritiesi

Contains 5 BNR repeats.
Contains 1 F5/8 type C domain.

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
2.60.120.260. 1 hit.
2.60.40.10. 1 hit.
InterProiIPR018905. A-galactase_NEW3.
IPR000421. Coagulation_fac_5/8-C_type_dom.
IPR008979. Galactose-bd-like.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
IPR006311. TAT_signal.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
PfamiPF00754. F5_F8_type_C. 1 hit.
PF10633. NPCBM_assoc. 1 hit.
[Graphical view]
SMARTiSM00231. FA58C. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF50939. SSF50939. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS50022. FA58C_3. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02834-1 [UniParc]FASTAAdd to Basket

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MTANPYLRRL PRRRAVSFLL APALAAATVA GASPAQAIAG APVPPGGEPL    50
YTEQDLAVNG REGFPNYRIP ALTVTPDGDL LASYDGRPTG IDAPGPNSIL 100
QRRSTDGGRT WGEQQVVSAG QTTAPIKGFS DPSYLVDRET GTIFNFHVYS 150
QRQGFAGSRP GTDPADPNVL HANVATSTDG GLTWSHRTIT ADITPDPGWR 200
SRFAASGEGI QLRYGPHAGR LIQQYTIINA AGAFQAVSVY SDDHGRTWRA 250
GEAVGVGMDE NKTVELSDGR VLLNSRDSAR SGYRKVAVST DGGHSYGPVT 300
IDRDLPDPTN NASIIRAFPD APAGSARAKV LLFSNAASQT SRSQGTIRMS 350
CDDGQTWPVS KVFQPGSMSY STLTALPDGT YGLLYEPGTG IRYANFNLAW 400
LGGICAPFTI PDVALEPGQQ VTVPVAVTNQ SGIAVPKPSL QLDASPDWQV 450
QGSVEPLMPG RQAKGQVTIT VPAGTTPGRY RVGATLRTSA GNASTTFTVT 500
VGLLDQARMS IADVDSEETA REDGRASNVI DGNPSTFWHT EWSRADAPGY 550
PHRISLDLGG THTISGLQYT RRQNSANEQV ADYEIYTSLN GTTWDGPVAS 600
GRFTTSLAPQ RAVFPARDAR YIRLVALSEQ TGHKYAAVAE LEVEGQR 647
Length:647
Mass (Da):68,830
Last modified:October 1, 1994 - v1
Checksum:iDCC1FE5BC935B8AD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D01045 Genomic DNA. Translation: BAA00852.1.
PIRiA45244.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D01045 Genomic DNA. Translation: BAA00852.1 .
PIRi A45244.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1EUR X-ray 1.82 A 43-407 [» ]
1EUS X-ray 2.00 A 43-407 [» ]
1EUT X-ray 2.50 A 43-647 [» ]
1EUU X-ray 2.50 A 43-647 [» ]
1W8N X-ray 2.10 A 47-647 [» ]
1W8O X-ray 1.70 A 47-647 [» ]
1WCQ X-ray 2.10 A/B/C 47-647 [» ]
2BER X-ray 1.80 A 47-647 [» ]
2BZD X-ray 2.00 A/B/C 47-647 [» ]
4J9T X-ray 1.40 A 47-407 [» ]
ProteinModelPortali Q02834.
SMRi Q02834. Positions 47-647.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM32. Carbohydrate-Binding Module Family 32.
GH33. Glycoside Hydrolase Family 33.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

SABIO-RK Q02834.

Miscellaneous databases

EvolutionaryTracei Q02834.

Family and domain databases

Gene3Di 2.120.10.10. 1 hit.
2.60.120.260. 1 hit.
2.60.40.10. 1 hit.
InterProi IPR018905. A-galactase_NEW3.
IPR000421. Coagulation_fac_5/8-C_type_dom.
IPR008979. Galactose-bd-like.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
IPR006311. TAT_signal.
[Graphical view ]
PANTHERi PTHR10628. PTHR10628. 1 hit.
Pfami PF00754. F5_F8_type_C. 1 hit.
PF10633. NPCBM_assoc. 1 hit.
[Graphical view ]
SMARTi SM00231. FA58C. 1 hit.
[Graphical view ]
SUPFAMi SSF49785. SSF49785. 1 hit.
SSF50939. SSF50939. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEi PS50022. FA58C_3. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, expression, and characterization of the Micromonospora viridifaciens neuraminidase gene in Streptomyces lividans."
    Sakurada K., Ohta T., Hasegawa M.
    J. Bacteriol. 174:6896-6903(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 38-647.
    Strain: ATCC 31146 / DSM 43909 / FD 23988 / JCM 3267.
  2. "The three domains of a bacterial sialidase: a beta-propeller, an immunoglobulin module and a galactose-binding jelly-roll."
    Gaskell A., Crenell S., Taylor G.
    Structure 3:1197-1205(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
    Strain: ATCC 31146 / DSM 43909 / FD 23988 / JCM 3267.

Entry informationi

Entry nameiNANH_MICVI
AccessioniPrimary (citable) accession number: Q02834
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: June 11, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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