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Protein

Sialidase

Gene

nedA

Organism
Micromonospora viridifaciens
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

To release sialic acids for use as carbon and energy sources for this non-pathogenic bacterium while in pathogenic microorganisms, sialidases have been suggested to be pathogenic factors.

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei68SubstrateBy similarity1
Active sitei92Proton acceptorBy similarity1
Active sitei260NucleophileSequence analysis1
Binding sitei276SubstrateBy similarity1
Active sitei370Nucleophile1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.18. 7474.
SABIO-RKQ02834.

Protein family/group databases

CAZyiCBM32. Carbohydrate-Binding Module Family 32.
GH33. Glycoside Hydrolase Family 33.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialidase (EC:3.2.1.18)
Alternative name(s):
Neuraminidase
Gene namesi
Name:nedA
OrganismiMicromonospora viridifaciens
Taxonomic identifieri1881 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicromonosporalesMicromonosporaceaeMicromonospora

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry databases

DrugBankiDB04465. Lactose.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 371 PublicationAdd BLAST37
ChainiPRO_000001203238 – 647SialidaseAdd BLAST610

Expressioni

Inductioni

By N-acetylneuraminic acid, colominic acid, and sialic acid.

Structurei

Secondary structure

1647
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi51 – 58Combined sources8
Beta strandi61 – 63Combined sources3
Beta strandi65 – 74Combined sources10
Beta strandi80 – 87Combined sources8
Beta strandi98 – 106Combined sources9
Beta strandi107 – 109Combined sources3
Beta strandi115 – 118Combined sources4
Beta strandi123 – 125Combined sources3
Beta strandi127 – 136Combined sources10
Turni138 – 140Combined sources3
Beta strandi143 – 152Combined sources10
Turni155 – 157Combined sources3
Beta strandi171 – 179Combined sources9
Beta strandi185 – 188Combined sources4
Helixi190 – 193Combined sources4
Beta strandi201 – 204Combined sources4
Beta strandi206 – 208Combined sources3
Turni216 – 219Combined sources4
Beta strandi221 – 228Combined sources8
Beta strandi234 – 243Combined sources10
Beta strandi260 – 265Combined sources6
Beta strandi271 – 275Combined sources5
Beta strandi282 – 291Combined sources10
Beta strandi300 – 306Combined sources7
Beta strandi313 – 318Combined sources6
Helixi327 – 329Combined sources3
Beta strandi331 – 336Combined sources6
Beta strandi339 – 352Combined sources14
Beta strandi359 – 367Combined sources9
Beta strandi371 – 375Combined sources5
Beta strandi381 – 385Combined sources5
Beta strandi388 – 396Combined sources9
Helixi398 – 401Combined sources4
Beta strandi413 – 415Combined sources3
Beta strandi420 – 428Combined sources9
Beta strandi431 – 433Combined sources3
Beta strandi435 – 437Combined sources3
Beta strandi439 – 443Combined sources5
Beta strandi448 – 454Combined sources7
Beta strandi462 – 470Combined sources9
Beta strandi478 – 488Combined sources11
Beta strandi491 – 503Combined sources13
Helixi506 – 508Combined sources3
Beta strandi510 – 515Combined sources6
Beta strandi520 – 522Combined sources3
Helixi526 – 530Combined sources5
Beta strandi543 – 545Combined sources3
Beta strandi552 – 571Combined sources20
Beta strandi576 – 578Combined sources3
Beta strandi582 – 593Combined sources12
Beta strandi595 – 602Combined sources8
Beta strandi610 – 626Combined sources17
Beta strandi637 – 645Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EURX-ray1.82A43-407[»]
1EUSX-ray2.00A43-407[»]
1EUTX-ray2.50A43-647[»]
1EUUX-ray2.50A43-647[»]
1W8NX-ray2.10A47-647[»]
1W8OX-ray1.70A47-647[»]
1WCQX-ray2.10A/B/C47-647[»]
2BERX-ray1.80A47-647[»]
2BZDX-ray2.00A/B/C47-647[»]
4J9TX-ray1.40A47-407[»]
ProteinModelPortaliQ02834.
SMRiQ02834.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ02834.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati102 – 113BNR 1Add BLAST12
Repeati175 – 186BNR 2Add BLAST12
Repeati239 – 250BNR 3Add BLAST12
Repeati287 – 298BNR 4Add BLAST12
Repeati348 – 359BNR 5Add BLAST12
Domaini496 – 646F5/8 type CPROSITE-ProRule annotationAdd BLAST151

Sequence similaritiesi

Belongs to the glycosyl hydrolase 33 family.Curated
Contains 5 BNR repeats.Curated
Contains 1 F5/8 type C domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
2.60.120.260. 1 hit.
2.60.40.10. 1 hit.
InterProiIPR018905. A-galactase_NEW3.
IPR000421. FA58C.
IPR008979. Galactose-bd-like.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
IPR006311. TAT_signal.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
PfamiPF13088. BNR_2. 1 hit.
PF00754. F5_F8_type_C. 1 hit.
PF10633. NPCBM_assoc. 1 hit.
[Graphical view]
SMARTiSM00231. FA58C. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF50939. SSF50939. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS50022. FA58C_3. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02834-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTANPYLRRL PRRRAVSFLL APALAAATVA GASPAQAIAG APVPPGGEPL
60 70 80 90 100
YTEQDLAVNG REGFPNYRIP ALTVTPDGDL LASYDGRPTG IDAPGPNSIL
110 120 130 140 150
QRRSTDGGRT WGEQQVVSAG QTTAPIKGFS DPSYLVDRET GTIFNFHVYS
160 170 180 190 200
QRQGFAGSRP GTDPADPNVL HANVATSTDG GLTWSHRTIT ADITPDPGWR
210 220 230 240 250
SRFAASGEGI QLRYGPHAGR LIQQYTIINA AGAFQAVSVY SDDHGRTWRA
260 270 280 290 300
GEAVGVGMDE NKTVELSDGR VLLNSRDSAR SGYRKVAVST DGGHSYGPVT
310 320 330 340 350
IDRDLPDPTN NASIIRAFPD APAGSARAKV LLFSNAASQT SRSQGTIRMS
360 370 380 390 400
CDDGQTWPVS KVFQPGSMSY STLTALPDGT YGLLYEPGTG IRYANFNLAW
410 420 430 440 450
LGGICAPFTI PDVALEPGQQ VTVPVAVTNQ SGIAVPKPSL QLDASPDWQV
460 470 480 490 500
QGSVEPLMPG RQAKGQVTIT VPAGTTPGRY RVGATLRTSA GNASTTFTVT
510 520 530 540 550
VGLLDQARMS IADVDSEETA REDGRASNVI DGNPSTFWHT EWSRADAPGY
560 570 580 590 600
PHRISLDLGG THTISGLQYT RRQNSANEQV ADYEIYTSLN GTTWDGPVAS
610 620 630 640
GRFTTSLAPQ RAVFPARDAR YIRLVALSEQ TGHKYAAVAE LEVEGQR
Length:647
Mass (Da):68,830
Last modified:October 1, 1994 - v1
Checksum:iDCC1FE5BC935B8AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D01045 Genomic DNA. Translation: BAA00852.1.
PIRiA45244.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D01045 Genomic DNA. Translation: BAA00852.1.
PIRiA45244.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EURX-ray1.82A43-407[»]
1EUSX-ray2.00A43-407[»]
1EUTX-ray2.50A43-647[»]
1EUUX-ray2.50A43-647[»]
1W8NX-ray2.10A47-647[»]
1W8OX-ray1.70A47-647[»]
1WCQX-ray2.10A/B/C47-647[»]
2BERX-ray1.80A47-647[»]
2BZDX-ray2.00A/B/C47-647[»]
4J9TX-ray1.40A47-407[»]
ProteinModelPortaliQ02834.
SMRiQ02834.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

DrugBankiDB04465. Lactose.

Protein family/group databases

CAZyiCBM32. Carbohydrate-Binding Module Family 32.
GH33. Glycoside Hydrolase Family 33.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.2.1.18. 7474.
SABIO-RKQ02834.

Miscellaneous databases

EvolutionaryTraceiQ02834.

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
2.60.120.260. 1 hit.
2.60.40.10. 1 hit.
InterProiIPR018905. A-galactase_NEW3.
IPR000421. FA58C.
IPR008979. Galactose-bd-like.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
IPR006311. TAT_signal.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
PfamiPF13088. BNR_2. 1 hit.
PF00754. F5_F8_type_C. 1 hit.
PF10633. NPCBM_assoc. 1 hit.
[Graphical view]
SMARTiSM00231. FA58C. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF50939. SSF50939. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS50022. FA58C_3. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNANH_MICVI
AccessioniPrimary (citable) accession number: Q02834
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 2, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.