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Protein

tRNA-splicing endonuclease subunit SEN54

Gene

SEN54

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. May be required to embody the molecular ruler of the complex.

Miscellaneous

The tRNA splicing endonuclease complex is present with 100 molecules/cell.1 Publication
Present with 531 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

Keywordsi

Biological processtRNA processing

Enzyme and pathway databases

BioCyciMetaCyc:G3O-33989-MONOMER.
YEAST:G3O-33989-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonuclease subunit SEN54
Alternative name(s):
Splicing endonuclease of 54 kDa
tRNA-intron endonuclease SEN54
Gene namesi
Name:SEN54
Ordered Locus Names:YPL083C
ORF Names:LPF3C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL083C.
SGDiS000006004. SEN54.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001940341 – 467tRNA-splicing endonuclease subunit SEN54Add BLAST467

Proteomic databases

MaxQBiQ02825.
PRIDEiQ02825.

Interactioni

Subunit structurei

tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34 and SEN54. Interacts directly with SEN2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SEN2P166584EBI-16829,EBI-16953

Protein-protein interaction databases

BioGridi36098. 110 interactors.
DIPiDIP-4452N.
IntActiQ02825. 5 interactors.
MINTiMINT-549039.
STRINGi4932.YPL083C.

Structurei

3D structure databases

ProteinModelPortaliQ02825.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi19 – 27Poly-Glu9
Compositional biasi353 – 356Poly-Asp4

Sequence similaritiesi

Belongs to the SEN54 family.Curated

Phylogenomic databases

HOGENOMiHOG000141804.
InParanoidiQ02825.
KOiK15326.
OMAiKPSTPFR.
OrthoDBiEOG092C3DBL.

Family and domain databases

InterProiView protein in InterPro
IPR024337. tRNA_splic_suSen54.
IPR024336. tRNA_splic_suSen54_N.
PANTHERiPTHR21027. PTHR21027. 1 hit.
PfamiView protein in Pfam
PF12928. tRNA_int_end_N2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q02825-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQFAGKKTDQ VTTSNPGFEE EEEEEEELQQ DWSQLASLVS KNAALSLPKR
60 70 80 90 100
GEKDYEPDGT NLQDLLLYNA SKAMFDTISD SIRGTTVKSE VRGYYVPHKH
110 120 130 140 150
QAVLLKPKGS FMQTMGRADS TGELWLDFHE FVYLAERGTI LPYYRLEAGS
160 170 180 190 200
NKSSKHETEI LLSMEDLYSL FSSQQEMDQY FVFAHLKRLG FILKPSNQEA
210 220 230 240 250
AVKTSFFPLK KQRSNLQAIT WRLLSLFKIQ ELSLFSGFFY SKWNFFFRKY
260 270 280 290 300
TTSPQLYQGL NRLVRSVAVP KNKKELLDAQ SDREFQKVKD IPLTFKVWKP
310 320 330 340 350
HSNFKKRDPG LPDFQVFVYN KNDDLQHFPT YKELRSMFSS LDYKFEFLSE
360 370 380 390 400
IEDDDDWETN SYVEDIPRKE YIHKRSAKSQ TEKSESSMKA SFQKKTAQSS
410 420 430 440 450
TKKKRKAYPP HIQQNRRLKT GYRSFIIAIM DNGLISFVKM SEADFGSESV
460
WYTPNTQKKV DQRWKKH
Length:467
Mass (Da):54,649
Last modified:November 1, 1996 - v1
Checksum:i363A703856DE724E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41849 Genomic DNA. Translation: AAB68256.1.
BK006949 Genomic DNA. Translation: DAA11350.1.
PIRiS61105.
RefSeqiNP_015242.1. NM_001183897.1.

Genome annotation databases

EnsemblFungiiYPL083C; YPL083C; YPL083C.
GeneIDi856022.
KEGGisce:YPL083C.

Similar proteinsi

Entry informationi

Entry nameiSEN54_YEAST
AccessioniPrimary (citable) accession number: Q02825
Secondary accession number(s): D6W3T4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: October 25, 2017
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names