Q02825 (SEN54_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA-splicing endonuclease subunit SEN54

Alternative name(s):
Splicing endonuclease of 54 kDa
tRNA-intron endonuclease SEN54

Gene names

Name:	SEN54
Ordered Locus Names:	YPL083C
ORF Names:	LPF3C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. May be required to embody the molecular ruler of the complex.
Subunit structure	tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34 and SEN54. Interacts directly with SEN2. Ref.3
Subcellular location	Nucleus. Endomembrane system; Peripheral membrane protein. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Note: The tRNA splicing endonuclease complex is predominantly associated with the outer membrane of mitochondria, suggesting that tRNA splicing mainly takes place on the mitochondrial surface. Ref.4
Miscellaneous	The tRNA splicing endonuclease complex is present with 100 molecules/cell. Ref.5 Present with 531 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the SEN54 family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane Nucleus
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	tRNA-type intron splice site recognition and cleavage Inferred from direct assay Ref.3. Source: SGD
Cellular component	endomembrane system Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from direct assay Ref.4. Source: SGD tRNA-intron endonuclease complex Inferred from direct assay Ref.3. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SEN2	P16658	4	EBI-16829,EBI-16953

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 467	467	tRNA-splicing endonuclease subunit SEN54		PRO_0000194034
Regions
	Compositional bias	19 – 27	9	Poly-Glu
	Compositional bias	353 – 356	4	Poly-Asp

Sequences

	Sequence		Length	Mass (Da)
	Q02825 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 363A703856DE724E	FASTA	467	54,649
	10 20 30 40 50 60 MQFAGKKTDQ VTTSNPGFEE EEEEEEELQQ DWSQLASLVS KNAALSLPKR GEKDYEPDGT 70 80 90 100 110 120 NLQDLLLYNA SKAMFDTISD SIRGTTVKSE VRGYYVPHKH QAVLLKPKGS FMQTMGRADS 130 140 150 160 170 180 TGELWLDFHE FVYLAERGTI LPYYRLEAGS NKSSKHETEI LLSMEDLYSL FSSQQEMDQY 190 200 210 220 230 240 FVFAHLKRLG FILKPSNQEA AVKTSFFPLK KQRSNLQAIT WRLLSLFKIQ ELSLFSGFFY 250 260 270 280 290 300 SKWNFFFRKY TTSPQLYQGL NRLVRSVAVP KNKKELLDAQ SDREFQKVKD IPLTFKVWKP 310 320 330 340 350 360 HSNFKKRDPG LPDFQVFVYN KNDDLQHFPT YKELRSMFSS LDYKFEFLSE IEDDDDWETN 370 380 390 400 410 420 SYVEDIPRKE YIHKRSAKSQ TEKSESSMKA SFQKKTAQSS TKKKRKAYPP HIQQNRRLKT 430 440 450 460 GYRSFIIAIM DNGLISFVKM SEADFGSESV WYTPNTQKKV DQRWKKH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The yeast tRNA splicing endonuclease: a tetrameric enzyme with two active site subunits homologous to the archaeal tRNA endonucleases." Trotta C.R., Miao F., Arn E.A., Stevens S.W., Ho C.K., Rauhut R., Abelson J.N. Cell 89:849-858(1997) [PubMed: 9200603] [Abstract] Cited for: CHARACTERIZATION, PROTEIN SEQUENCE OF 322-332, SUBUNIT.
[4]	"Possibility of cytoplasmic pre-tRNA splicing: the yeast tRNA splicing endonuclease mainly localizes on the mitochondria." Yoshihisa T., Yunoki-Esaki K., Ohshima C., Tanaka N., Endo T. Mol. Biol. Cell 14:3266-3279(2003) [PubMed: 12925762] [Abstract] Cited for: SUBCELLULAR LOCATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U41849 Genomic DNA. Translation: AAB68256.1. BK006949 Genomic DNA. Translation: DAA11350.1.
PIR	S61105.
RefSeq	NP_015242.1. NM_001183897.1.
3D structure databases
ProteinModelPortal	Q02825.
SMR	Q02825. Positions 115-194.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4452N.
IntAct	Q02825. 5 interactions.
MINT	MINT-549039.
STRING	Q02825.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL083C; YPL083C; YPL083C.
GeneID	856022.
KEGG	sce:YPL083C.
NMPDR	fig|4932.3.peg.6375.
Organism-specific databases
CYGD	YPL083c.
SGD	S000006004. SEN54.
Phylogenomic databases
eggNOG	fuNOG04324.
GeneTree	EFGT00050000001538.
HOGENOM	HBG203905.
OMA	DYEPDGT.
OrthoDB	EOG4XD70S.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-16151.
Gene expression databases
ArrayExpress	Q02825.
Genevestigator	Q02825.
GermOnline	YPL083C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR024337. tRNA_splic_suSen54. IPR024336. tRNA_splic_suSen54_N. [Graphical view]
KO	K15326.
PANTHER	PTHR21027. PTHR21027. 1 hit.
Pfam	PF12928. tRNA_int_end_N2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980932.

Entry information

Entry name

SEN54_YEAST

Accession

Primary (citable) accession number: Q02825
Secondary accession number(s): D6W3T4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program