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Protein

Nucleobindin-1

Gene

Nucb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Major calcium-binding protein of the Golgi. May have a role in calcium homeostasis (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi171 – 217Sequence analysisAdd BLAST47
Calcium bindingi252 – 2631PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi304 – 3152PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

Complete GO annotation...

Keywords - Ligandi

Calcium, DNA-binding, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleobindin-1
Alternative name(s):
CALNUC
Gene namesi
Name:Nucb1
Synonyms:Nuc, Nucb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:97388. Nucb1.

Subcellular locationi

  • Golgi apparatuscis-Golgi network membrane By similarity; Peripheral membrane protein By similarity; Lumenal side By similarity
  • Cytoplasm By similarity
  • Secreted By similarity

  • Note: A small fraction of the protein may be cytoplasmic. In bone at least part of it is found in the extracellular matrix and in the culture medium of calvaria explants (By similarity). In lupus prone, but not in normal mice, at least part of it is in the serum where it induces the formation of autoantibodies including anti-DNA antibodies.By similarityCurated

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000000416326 – 459Nucleobindin-1Add BLAST434

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei85PhosphoserineCombined sources1 Publication1
Modified residuei147PhosphothreonineBy similarity1
Modified residuei368PhosphoserineCombined sources1
Modified residuei456PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ02819.
MaxQBiQ02819.
PaxDbiQ02819.
PeptideAtlasiQ02819.
PRIDEiQ02819.

PTM databases

iPTMnetiQ02819.
PhosphoSitePlusiQ02819.

Expressioni

Tissue specificityi

Lymphoid cells as well as other somatic cells, such as liver and kidney cells.

Gene expression databases

BgeeiENSMUSG00000030824.
CleanExiMM_NUCB1.
GenevisibleiQ02819. MM.

Interactioni

Subunit structurei

Interacts with GNAI2 and GNAI3.By similarity

Protein-protein interaction databases

BioGridi201875. 6 interactors.
IntActiQ02819. 8 interactors.
MINTiMINT-1718643.
STRINGi10090.ENSMUSP00000033096.

Structurei

3D structure databases

ProteinModelPortaliQ02819.
SMRiQ02819.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini239 – 274EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini291 – 326EF-hand 2PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni227 – 320Binds to GNAI2 and GNAI3By similarityAdd BLAST94

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili149 – 217Sequence analysisAdd BLAST69
Coiled coili340 – 407Sequence analysisAdd BLAST68

Domaini

The EF-hand domains are unfolded in the absence of Ca2+ and fold upon Ca2+ addition.By similarity

Sequence similaritiesi

Belongs to the nucleobindin family.Curated
Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, Signal

Phylogenomic databases

eggNOGiKOG3866. Eukaryota.
ENOG410XW7D. LUCA.
GeneTreeiENSGT00390000001927.
HOGENOMiHOG000007216.
HOVERGENiHBG052685.
InParanoidiQ02819.
KOiK20371.
OMAiAKMDAEQ.
OrthoDBiEOG091G0HG8.
PhylomeDBiQ02819.
TreeFamiTF323218.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02819-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTSVPRGAP FLLLPPLLML SAVLAVPVDR AAPPQEDSQA TETPDTGLYY
60 70 80 90 100
HRYLQEVINV LETDGHFREK LQAANAEDIK SGKLSQELDF VSHNVRTKLD
110 120 130 140 150
ELKRQEVSRL RMLLKAKMDA KQEPNLQVDH MNLLKQFEHL DPQNQHTFEA
160 170 180 190 200
RDLELLIQTA TRDLAQYDAA HHEEFKRYEM LKEHERRRYL ESLGEEQRKE
210 220 230 240 250
AERKLQEQQR RHREHPKVNV PGSQAQLKEV WEELDGLDPN RFNPKTFFIL
260 270 280 290 300
HDINSDGVLD EQELEALFTK ELEKVYDPKN EEDDMREMEE ERLRMREHVM
310 320 330 340 350
KNVDTNQDRL VTLEEFLAST QRKEFGDTGE GWKTVEMSPA YTEEELKRFE
360 370 380 390 400
EELAAREAEL NARAQRLSQE TEALGRSQDR LEAQKRELQQ AVLQMEQRKQ
410 420 430 440 450
QLQEQSAPPS KPDGQLQFRA DTDDAPVPAP AGDQKDVPAS EKKVPEQPPE

LPQLDSQHL
Length:459
Mass (Da):53,409
Last modified:November 2, 2001 - v2
Checksum:i1CC1102D216CC60B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti42E → D in BAB25383 (PubMed:16141072).Curated1
Sequence conflicti146H → E AA sequence (PubMed:1520323).Curated1
Sequence conflicti151R → I AA sequence (PubMed:1520323).Curated1
Sequence conflicti390 – 393Missing in AAA39842 (PubMed:1520323).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96823 mRNA. Translation: AAA39842.1.
AK002630 mRNA. No translation available.
AK004886 mRNA. Translation: BAB23644.1.
AK007976 mRNA. Translation: BAB25383.1.
AK140710 mRNA. Translation: BAE24451.1.
AK160981 mRNA. Translation: BAE36129.1.
AK164088 mRNA. Translation: BAE37619.1.
BC072554 mRNA. Translation: AAH72554.1.
CCDSiCCDS52247.1.
PIRiJC1224.
RefSeqiNP_001157134.1. NM_001163662.1.
NP_032775.1. NM_008749.2.
XP_006540759.1. XM_006540696.2.
XP_017177513.1. XM_017322024.1.
UniGeneiMm.258923.

Genome annotation databases

EnsembliENSMUST00000211765; ENSMUSP00000147383; ENSMUSG00000030824.
GeneIDi18220.
KEGGimmu:18220.
UCSCiuc009gvm.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96823 mRNA. Translation: AAA39842.1.
AK002630 mRNA. No translation available.
AK004886 mRNA. Translation: BAB23644.1.
AK007976 mRNA. Translation: BAB25383.1.
AK140710 mRNA. Translation: BAE24451.1.
AK160981 mRNA. Translation: BAE36129.1.
AK164088 mRNA. Translation: BAE37619.1.
BC072554 mRNA. Translation: AAH72554.1.
CCDSiCCDS52247.1.
PIRiJC1224.
RefSeqiNP_001157134.1. NM_001163662.1.
NP_032775.1. NM_008749.2.
XP_006540759.1. XM_006540696.2.
XP_017177513.1. XM_017322024.1.
UniGeneiMm.258923.

3D structure databases

ProteinModelPortaliQ02819.
SMRiQ02819.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201875. 6 interactors.
IntActiQ02819. 8 interactors.
MINTiMINT-1718643.
STRINGi10090.ENSMUSP00000033096.

PTM databases

iPTMnetiQ02819.
PhosphoSitePlusiQ02819.

Proteomic databases

EPDiQ02819.
MaxQBiQ02819.
PaxDbiQ02819.
PeptideAtlasiQ02819.
PRIDEiQ02819.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000211765; ENSMUSP00000147383; ENSMUSG00000030824.
GeneIDi18220.
KEGGimmu:18220.
UCSCiuc009gvm.2. mouse.

Organism-specific databases

CTDi4924.
MGIiMGI:97388. Nucb1.

Phylogenomic databases

eggNOGiKOG3866. Eukaryota.
ENOG410XW7D. LUCA.
GeneTreeiENSGT00390000001927.
HOGENOMiHOG000007216.
HOVERGENiHBG052685.
InParanoidiQ02819.
KOiK20371.
OMAiAKMDAEQ.
OrthoDBiEOG091G0HG8.
PhylomeDBiQ02819.
TreeFamiTF323218.

Miscellaneous databases

PROiQ02819.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030824.
CleanExiMM_NUCB1.
GenevisibleiQ02819. MM.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUCB1_MOUSE
AccessioniPrimary (citable) accession number: Q02819
Secondary accession number(s): Q3TU42
, Q9CWA1, Q9D8J4, Q9DBL3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 2, 2001
Last modified: November 2, 2016
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Discovered as DNA-binding protein in the serum of lupus-prone mice.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.