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Q02818

- NUCB1_HUMAN

UniProt

Q02818 - NUCB1_HUMAN

Protein

Nucleobindin-1

Gene

NUCB1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 4 (07 Mar 2006)
      Previous versions | rss
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    Functioni

    Major calcium-binding protein of the Golgi. May have a role in calcium homeostasis By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi172 – 21847Sequence AnalysisAdd
    BLAST
    Calcium bindingi253 – 264121Add
    BLAST
    Calcium bindingi305 – 316122Add
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. DNA binding Source: ProtInc
    3. protein binding Source: IntAct

    Keywords - Ligandi

    Calcium, DNA-binding, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nucleobindin-1
    Alternative name(s):
    CALNUC
    Gene namesi
    Name:NUCB1
    Synonyms:NUC
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:8043. NUCB1.

    Subcellular locationi

    Golgi apparatuscis-Golgi network membrane By similarity; Peripheral membrane protein By similarity; Lumenal side By similarity. Cytoplasm By similarity
    Note: A small fraction of the protein may be cytoplasmic.By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB
    2. extracellular space Source: UniProt
    3. extracellular vesicular exosome Source: UniProt
    4. Golgi apparatus Source: UniProtKB-SubCell
    5. membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA31825.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Sequence AnalysisAdd
    BLAST
    Chaini27 – 461435Nucleobindin-1PRO_0000004162Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei86 – 861PhosphoserineBy similarity
    Modified residuei369 – 3691PhosphoserineBy similarity

    Post-translational modificationi

    O-glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiQ02818.
    PaxDbiQ02818.
    PRIDEiQ02818.

    PTM databases

    PhosphoSiteiQ02818.

    Expressioni

    Tissue specificityi

    Expressed both in fetal and adult heart, lung, liver, kidney and brain, and in adult skeletal muscle, placenta and pancreas.

    Gene expression databases

    ArrayExpressiQ02818.
    BgeeiQ02818.
    CleanExiHS_NUCB1.
    GenevestigatoriQ02818.

    Organism-specific databases

    HPAiHPA008176.

    Interactioni

    Subunit structurei

    Interacts with GNAI2 and GNAI3.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Q99IB83EBI-2622179,EBI-6927928From a different organism.

    Protein-protein interaction databases

    BioGridi110978. 21 interactions.
    IntActiQ02818. 15 interactions.
    MINTiMINT-2805455.
    STRINGi9606.ENSP00000263273.

    Structurei

    Secondary structure

    1
    461
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi245 – 2528
    Beta strandi258 – 2614
    Helixi262 – 27413
    Helixi285 – 2884
    Helixi291 – 30313
    Beta strandi309 – 3135
    Helixi314 – 3229

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1SNLNMR-A228-326[»]
    ProteinModelPortaliQ02818.
    SMRiQ02818. Positions 228-326.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ02818.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini240 – 27536EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini292 – 32736EF-hand 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni42 – 5110O-glycosylated at one site
    Regioni228 – 32194Binds to GNAI2 and GNAI3By similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili150 – 21869Sequence AnalysisAdd
    BLAST
    Coiled coili341 – 40767Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi401 – 4077Poly-Gln

    Domaini

    The EF-hand domains are unfolded in the absence of Ca2+ and fold upon Ca2+ addition.

    Sequence similaritiesi

    Belongs to the nucleobindin family.Curated
    Contains 2 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG262100.
    HOGENOMiHOG000007216.
    HOVERGENiHBG052685.
    InParanoidiQ02818.
    OMAiMDAEQEP.
    OrthoDBiEOG7WQ7SZ.
    PhylomeDBiQ02818.
    TreeFamiTF323218.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view]
    PfamiPF13499. EF-hand_7. 1 hit.
    [Graphical view]
    PROSITEiPS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q02818-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPPSGPRGTL LLLPLLLLLL LRAVLAVPLE RGAPNKEETP ATESPDTGLY    50
    YHRYLQEVID VLETDGHFRE KLQAANAEDI KSGKLSRELD FVSHHVRTKL 100
    DELKRQEVSR LRMLLKAKMD AEQDPNVQVD HLNLLKQFEH LDPQNQHTFE 150
    ARDLELLIQT ATRDLAQYDA AHHEEFKRYE MLKEHERRRY LESLGEEQRK 200
    EAERKLEEQQ RRHREHPKVN VPGSQAQLKE VWEELDGLDP NRFNPKTFFI 250
    LHDINSDGVL DEQELEALFT KELEKVYDPK NEEDDMREME EERLRMREHV 300
    MKNVDTNQDR LVTLEEFLAS TQRKEFGDTG EGWETVEMHP AYTEEELRRF 350
    EEELAAREAE LNAKAQRLSQ ETEALGRSQG RLEAQKRELQ QAVLHMEQRK 400
    QQQQQQQGHK APAAHPEGQL KFHPDTDDVP VPAPAGDQKE VDTSEKKLLE 450
    RLPEVEVPQH L 461
    Length:461
    Mass (Da):53,879
    Last modified:March 7, 2006 - v4
    Checksum:i913C4B87C2A644C5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti299 – 3002HV → QL in AAA36383. (PubMed:1520323)Curated
    Sequence conflicti385 – 3851Q → K in AAA36383. (PubMed:1520323)Curated
    Sequence conflicti385 – 3851Q → K in AAB60431. (PubMed:8661046)Curated
    Sequence conflicti390 – 3912QQ → LL in AAA36383. (PubMed:1520323)Curated
    Sequence conflicti390 – 3912QQ → LL in AAB60431. (PubMed:8661046)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti13 – 142LP → S.
    VAR_012151
    Natural varianti338 – 3381M → V.
    Corresponds to variant rs35456905 [ dbSNP | Ensembl ].
    VAR_061087
    Natural varianti399 – 3991R → Q.1 Publication
    VAR_012152

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96824 mRNA. Translation: AAA36383.1.
    U31342
    , U31336, U31337, U31338, U31340, U31341 Genomic DNA. Translation: AAB60431.1.
    BT009828 mRNA. Translation: AAP88830.1.
    AK315422 mRNA. Translation: BAG37811.1.
    CH471177 Genomic DNA. Translation: EAW52411.1.
    BC002356 mRNA. Translation: AAH02356.1.
    CCDSiCCDS12740.1.
    RefSeqiNP_006175.2. NM_006184.5.
    UniGeneiHs.631602.

    Genome annotation databases

    EnsembliENST00000405315; ENSP00000385923; ENSG00000104805.
    ENST00000407032; ENSP00000385211; ENSG00000104805.
    GeneIDi4924.
    KEGGihsa:4924.
    UCSCiuc002pla.3. human.

    Polymorphism databases

    DMDMi90110780.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96824 mRNA. Translation: AAA36383.1 .
    U31342
    , U31336 , U31337 , U31338 , U31340 , U31341 Genomic DNA. Translation: AAB60431.1 .
    BT009828 mRNA. Translation: AAP88830.1 .
    AK315422 mRNA. Translation: BAG37811.1 .
    CH471177 Genomic DNA. Translation: EAW52411.1 .
    BC002356 mRNA. Translation: AAH02356.1 .
    CCDSi CCDS12740.1.
    RefSeqi NP_006175.2. NM_006184.5.
    UniGenei Hs.631602.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1SNL NMR - A 228-326 [» ]
    ProteinModelPortali Q02818.
    SMRi Q02818. Positions 228-326.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 110978. 21 interactions.
    IntActi Q02818. 15 interactions.
    MINTi MINT-2805455.
    STRINGi 9606.ENSP00000263273.

    PTM databases

    PhosphoSitei Q02818.

    Polymorphism databases

    DMDMi 90110780.

    Proteomic databases

    MaxQBi Q02818.
    PaxDbi Q02818.
    PRIDEi Q02818.

    Protocols and materials databases

    DNASUi 4924.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000405315 ; ENSP00000385923 ; ENSG00000104805 .
    ENST00000407032 ; ENSP00000385211 ; ENSG00000104805 .
    GeneIDi 4924.
    KEGGi hsa:4924.
    UCSCi uc002pla.3. human.

    Organism-specific databases

    CTDi 4924.
    GeneCardsi GC19P049403.
    HGNCi HGNC:8043. NUCB1.
    HPAi HPA008176.
    MIMi 601323. gene.
    neXtProti NX_Q02818.
    PharmGKBi PA31825.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG262100.
    HOGENOMi HOG000007216.
    HOVERGENi HBG052685.
    InParanoidi Q02818.
    OMAi MDAEQEP.
    OrthoDBi EOG7WQ7SZ.
    PhylomeDBi Q02818.
    TreeFami TF323218.

    Miscellaneous databases

    ChiTaRSi NUCB1. human.
    EvolutionaryTracei Q02818.
    GeneWikii NUCB1.
    GenomeRNAii 4924.
    NextBioi 18963.
    PROi Q02818.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q02818.
    Bgeei Q02818.
    CleanExi HS_NUCB1.
    Genevestigatori Q02818.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view ]
    Pfami PF13499. EF-hand_7. 1 hit.
    [Graphical view ]
    PROSITEi PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of nucleobindin, a novel DNA-binding protein that contains both a signal peptide and a leucine zipper structure."
      Miura K., Titani K., Kurosawa Y., Kanai Y.
      Biochem. Biophys. Res. Commun. 187:375-380(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Organization of the human gene for nucleobindin (NUC) and its chromosomal assignment to 19q13.2-q13.4."
      Miura K., Hirai M., Kanai Y., Kurosawa Y.
      Genomics 34:181-186(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS 13-LEU-PRO-14 DELINS SER AND GLN-399, SEQUENCE REVISION TO 299-300.
      Tissue: Placenta.
    3. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Umbilical cord blood.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung.
    7. "Interaction of the protein nucleobindin with G alpha i2, as revealed by the yeast two-hybrid system."
      Mochizuki N., Hibi M., Kanai Y., Insel P.A.
      FEBS Lett. 373:155-158(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, INTERACTION WITH GNAI2.
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins."
      Halim A., Ruetschi U., Larson G., Nilsson J.
      J. Proteome Res. 12:573-584(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION, IDENTIFICATION BY MASS SPECTROMETRY.
    10. "Structural studies on the Ca2+-binding domain of human nucleobindin (Calnuc)."
      de Alba E., Tjandra N.
      Biochemistry 43:10039-10049(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 228-326, CALCIUM-BINDING.

    Entry informationi

    Entry nameiNUCB1_HUMAN
    AccessioniPrimary (citable) accession number: Q02818
    Secondary accession number(s): B2RD64
    , Q15838, Q7Z4J7, Q9BUR1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: March 7, 2006
    Last modified: October 1, 2014
    This is version 136 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    Discovered as DNA-binding protein in the serum of lupus-prone mice.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3