Q02818 (NUCB1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleobindin-1

Alternative name(s):
CALNUC

Gene names

Name:	NUCB1
Synonyms:	NUC

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Major calcium-binding protein of the Golgi. May have a role in calcium homeostasis By similarity.
Subunit structure	Interacts with GNAI2 and GNAI3. Ref.7
Subcellular location	Golgi apparatus › cis-Golgi network membrane; Peripheral membrane protein; Lumenal side By similarity. Cytoplasm By similarity. Note: A small fraction of the protein may be cytoplasmic By similarity.
Tissue specificity	Expressed both in fetal and adult heart, lung, liver, kidney and brain, and in adult skeletal muscle, placenta and pancreas.
Domain	The EF-hand domains are unfolded in the absence of Ca2+ and fold upon Ca2+ addition.
Post-translational modification	O-glycosylated. Ref.9
Miscellaneous	Discovered as DNA-binding protein in the serum of lupus-prone mice.
Sequence similarities	Belongs to the nucleobindin family. Contains 2 EF-hand domains.

Ontologies

Keywords
Cellular component	Cytoplasm Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Coiled coil Repeat Signal
Ligand	Calcium DNA-binding Metal-binding
PTM	Glycoprotein Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	Golgi apparatus Inferred from direct assay. Source: HPA endoplasmic reticulum-Golgi intermediate compartment Inferred from direct assay PubMed 15308636. Source: UniProtKB extracellular space Traceable author statement Ref.1. Source: ProtInc membrane Inferred from electronic annotation. Source: UniProtKB-KW microtubule cytoskeleton Inferred from direct assay. Source: HPA
Molecular_function	DNA binding Traceable author statement Ref.1. Source: ProtInc calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 26

26

Potential

Chain

27 – 461

435

Nucleobindin-1

PRO_0000004162

Regions

Domain

240 – 275

36

EF-hand 1

Domain

292 – 327

36

EF-hand 2

DNA binding

172 – 218

47

Potential

Calcium binding

253 – 264

12

1 Ref.10

Calcium binding

305 – 316

12

2 Ref.10

Region

42 – 51

10

O-glycosylated at one site

Region

228 – 321

94

Binds to GNAI2 and GNAI3 By similarity

Coiled coil

150 – 218

69

Potential

Coiled coil

341 – 407

67

Potential

Compositional bias

401 – 407

7

Poly-Gln

Amino acid modifications

Modified residue

82

1

Phosphoserine By similarity

Modified residue

86

1

Phosphoserine By similarity

Modified residue

369

1

Phosphoserine By similarity

Modified residue

372

1

Phosphothreonine By similarity

Natural variations

Natural variant

13 – 14

2

LP → S.

VAR_012151

Natural variant

338

1

M → V.
Corresponds to variant rs35456905 [ dbSNP | Ensembl ].

VAR_061087

Natural variant

399

1

R → Q. Ref.2

VAR_012152

Experimental info

Sequence conflict

299 – 300

2

HV → QL in AAA36383. Ref.1

Sequence conflict

385

1

Q → K in AAA36383. Ref.1

Sequence conflict

385

1

Q → K in AAB60431. Ref.2

Sequence conflict

390 – 391

2

QQ → LL in AAA36383. Ref.1

Sequence conflict

390 – 391

2

QQ → LL in AAB60431. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

461

Helix Strand Turn

Details...

Helix

245 – 252

8

Beta strand

258 – 261

4

Helix

262 – 274

13

Helix

285 – 288

4

Helix

291 – 303

13

Beta strand

309 – 313

5

Helix

314 – 322

9

Sequences

	Sequence		Length	Mass (Da)
	Q02818 [UniParc]. Last modified March 7, 2006. Version 4. Checksum: 913C4B87C2A644C5	FASTA	461	53,879
	10 20 30 40 50 60 MPPSGPRGTL LLLPLLLLLL LRAVLAVPLE RGAPNKEETP ATESPDTGLY YHRYLQEVID 70 80 90 100 110 120 VLETDGHFRE KLQAANAEDI KSGKLSRELD FVSHHVRTKL DELKRQEVSR LRMLLKAKMD 130 140 150 160 170 180 AEQDPNVQVD HLNLLKQFEH LDPQNQHTFE ARDLELLIQT ATRDLAQYDA AHHEEFKRYE 190 200 210 220 230 240 MLKEHERRRY LESLGEEQRK EAERKLEEQQ RRHREHPKVN VPGSQAQLKE VWEELDGLDP 250 260 270 280 290 300 NRFNPKTFFI LHDINSDGVL DEQELEALFT KELEKVYDPK NEEDDMREME EERLRMREHV 310 320 330 340 350 360 MKNVDTNQDR LVTLEEFLAS TQRKEFGDTG EGWETVEMHP AYTEEELRRF EEELAAREAE 370 380 390 400 410 420 LNAKAQRLSQ ETEALGRSQG RLEAQKRELQ QAVLHMEQRK QQQQQQQGHK APAAHPEGQL 430 440 450 460 KFHPDTDDVP VPAPAGDQKE VDTSEKKLLE RLPEVEVPQH L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of nucleobindin, a novel DNA-binding protein that contains both a signal peptide and a leucine zipper structure." Miura K., Titani K., Kurosawa Y., Kanai Y. Biochem. Biophys. Res. Commun. 187:375-380(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Organization of the human gene for nucleobindin (NUC) and its chromosomal assignment to 19q13.2-q13.4." Miura K., Hirai M., Kanai Y., Kurosawa Y. Genomics 34:181-186(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS 13-LEU-PRO-14 DELINS SER AND GLN-399, SEQUENCE REVISION TO 299-300. Tissue: Placenta.
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Umbilical cord blood.
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[7]	"Interaction of the protein nucleobindin with G alpha i2, as revealed by the yeast two-hybrid system." Mochizuki N., Hibi M., Kanai Y., Insel P.A. FEBS Lett. 373:155-158(1995) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION, INTERACTION WITH GNAI2.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins." Halim A., Ruetschi U., Larson G., Nilsson J. J. Proteome Res. 12:573-584(2013) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION, MASS SPECTROMETRY.
[10]	"Structural studies on the Ca2+-binding domain of human nucleobindin (Calnuc)." de Alba E., Tjandra N. Biochemistry 43:10039-10049(2004) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 228-326, CALCIUM-BINDING.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M96824 mRNA. Translation: AAA36383.1.
U31342 , U31336, U31337, U31338, U31340, U31341 Genomic DNA. Translation: AAB60431.1.
BT009828 mRNA. Translation: AAP88830.1.
AK315422 mRNA. Translation: BAG37811.1.
CH471177 Genomic DNA. Translation: EAW52411.1.
BC002356 mRNA. Translation: AAH02356.1.

IPI

IPI00295542.

RefSeq

NP_006175.2. NM_006184.5.

UniGene

Hs.631602.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1SNL	NMR	-	A	228-326	[»]

ProteinModelPortal

Q02818.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q02818. 10 interactions.

MINT

MINT-2805455.

STRING

9606.ENSP00000263273.

PTM databases

PhosphoSite

Q02818.

Polymorphism databases

DMDM

90110780.

Proteomic databases

PaxDb

Q02818.

PRIDE

Q02818.

Protocols and materials databases

DNASU

4924.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000263273; ENSP00000263273; ENSG00000104805.
ENST00000405315; ENSP00000385923; ENSG00000104805.
ENST00000407032; ENSP00000385211; ENSG00000104805.

GeneID

4924.

KEGG

hsa:4924.

UCSC

uc002pla.3. human.

Organism-specific databases

CTD

4924.

GeneCards

GC19P049403.

HGNC

HGNC:8043. NUCB1.

HPA

HPA008176.

MIM

601323. gene.

neXtProt

NX_Q02818.

PharmGKB

PA31825.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG262100.

HOGENOM

HOG000007216.

HOVERGEN

HBG052685.

InParanoid

Q02818.

OMA

AKMDAEQ.

OrthoDB

EOG4N04F6.

PhylomeDB

Q02818.

Gene expression databases

ArrayExpress

Q02818.

Bgee

Q02818.

CleanEx

HS_NUCB1.

Genevestigator

Q02818.

GermOnline

ENSG00000104805. Homo sapiens.

Family and domain databases

Gene3D

1.10.238.10. 1 hit.

InterPro

IPR011992. EF-hand-like_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]

Pfam

PF13499. EF_hand_5. 1 hit.
[Graphical view]

PROSITE

PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

NUCB1. human.

EvolutionaryTrace

Q02818.

GenomeRNAi

4924.

NextBio

18963.

SOURCE

Search...

Entry information

Entry name

NUCB1_HUMAN

Accession

Primary (citable) accession number: Q02818
Secondary accession number(s): B2RD64 , Q15838, Q7Z4J7, Q9BUR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	March 7, 2006
Last modified:	May 1, 2013
This is version 122 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.