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Protein

Nucleobindin-1

Gene

NUCB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major calcium-binding protein of the Golgi. May have a role in calcium homeostasis (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi172 – 218Sequence analysisAdd BLAST47
Calcium bindingi253 – 26411 PublicationAdd BLAST12
Calcium bindingi305 – 31621 PublicationAdd BLAST12

GO - Molecular functioni

  • calcium ion binding Source: Ensembl
  • DNA binding Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Calcium, DNA-binding, Metal-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000104805-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleobindin-1
Alternative name(s):
CALNUC
Gene namesi
Name:NUCB1
Synonyms:NUC
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:8043. NUCB1.

Subcellular locationi

  • Golgi apparatuscis-Golgi network membrane By similarity; Peripheral membrane protein By similarity; Lumenal side By similarity
  • Cytoplasm By similarity
  • Secreted By similarity

  • Note: A small fraction of the protein may be cytoplasmic.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi4924.
OpenTargetsiENSG00000104805.
PharmGKBiPA31825.

Chemistry databases

GuidetoPHARMACOLOGYi2871.

Polymorphism and mutation databases

BioMutaiNUCB1.
DMDMi90110780.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000000416227 – 461Nucleobindin-1Add BLAST435

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei86Phosphoserine; by FAM20C1 Publication1
Modified residuei148Phosphothreonine; by FAM20CCombined sources1 Publication1
Modified residuei369Phosphoserine; by FAM20C1 Publication1

Post-translational modificationi

O-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ02818.
MaxQBiQ02818.
PaxDbiQ02818.
PeptideAtlasiQ02818.
PRIDEiQ02818.

PTM databases

iPTMnetiQ02818.
PhosphoSitePlusiQ02818.

Expressioni

Tissue specificityi

Expressed both in fetal and adult heart, lung, liver, kidney and brain, and in adult skeletal muscle, placenta and pancreas.

Gene expression databases

BgeeiENSG00000104805.
CleanExiHS_NUCB1.
ExpressionAtlasiQ02818. baseline and differential.
GenevisibleiQ02818. HS.

Organism-specific databases

HPAiHPA008176.

Interactioni

Subunit structurei

Interacts with GNAI2 and GNAI3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Q99IB83EBI-2622179,EBI-6927928From a different organism.

Protein-protein interaction databases

BioGridi110978. 33 interactors.
IntActiQ02818. 16 interactors.
MINTiMINT-2805455.
STRINGi9606.ENSP00000263273.

Structurei

Secondary structure

1461
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi245 – 252Combined sources8
Beta strandi258 – 261Combined sources4
Helixi262 – 274Combined sources13
Helixi285 – 288Combined sources4
Helixi291 – 303Combined sources13
Beta strandi309 – 313Combined sources5
Helixi314 – 322Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SNLNMR-A228-326[»]
ProteinModelPortaliQ02818.
SMRiQ02818.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ02818.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini240 – 275EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini292 – 327EF-hand 2PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni42 – 51O-glycosylated at one site10
Regioni228 – 321Binds to GNAI2 and GNAI3By similarityAdd BLAST94

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili150 – 218Sequence analysisAdd BLAST69
Coiled coili341 – 407Sequence analysisAdd BLAST67

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi401 – 407Poly-Gln7

Domaini

The EF-hand domains are unfolded in the absence of Ca2+ and fold upon Ca2+ addition.

Sequence similaritiesi

Belongs to the nucleobindin family.Curated
Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, Signal

Phylogenomic databases

eggNOGiKOG3866. Eukaryota.
ENOG410XW7D. LUCA.
GeneTreeiENSGT00390000001927.
HOGENOMiHOG000007216.
HOVERGENiHBG052685.
InParanoidiQ02818.
KOiK20371.
OMAiAKMDAEQ.
OrthoDBiEOG091G0HG8.
PhylomeDBiQ02818.
TreeFamiTF323218.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02818-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPSGPRGTL LLLPLLLLLL LRAVLAVPLE RGAPNKEETP ATESPDTGLY
60 70 80 90 100
YHRYLQEVID VLETDGHFRE KLQAANAEDI KSGKLSRELD FVSHHVRTKL
110 120 130 140 150
DELKRQEVSR LRMLLKAKMD AEQDPNVQVD HLNLLKQFEH LDPQNQHTFE
160 170 180 190 200
ARDLELLIQT ATRDLAQYDA AHHEEFKRYE MLKEHERRRY LESLGEEQRK
210 220 230 240 250
EAERKLEEQQ RRHREHPKVN VPGSQAQLKE VWEELDGLDP NRFNPKTFFI
260 270 280 290 300
LHDINSDGVL DEQELEALFT KELEKVYDPK NEEDDMREME EERLRMREHV
310 320 330 340 350
MKNVDTNQDR LVTLEEFLAS TQRKEFGDTG EGWETVEMHP AYTEEELRRF
360 370 380 390 400
EEELAAREAE LNAKAQRLSQ ETEALGRSQG RLEAQKRELQ QAVLHMEQRK
410 420 430 440 450
QQQQQQQGHK APAAHPEGQL KFHPDTDDVP VPAPAGDQKE VDTSEKKLLE
460
RLPEVEVPQH L
Length:461
Mass (Da):53,879
Last modified:March 7, 2006 - v4
Checksum:i913C4B87C2A644C5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti299 – 300HV → QL in AAA36383 (PubMed:1520323).Curated2
Sequence conflicti385Q → K in AAA36383 (PubMed:1520323).Curated1
Sequence conflicti385Q → K in AAB60431 (PubMed:8661046).Curated1
Sequence conflicti390 – 391QQ → LL in AAA36383 (PubMed:1520323).Curated2
Sequence conflicti390 – 391QQ → LL in AAB60431 (PubMed:8661046).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01215113 – 14LP → S.1 Publication2
Natural variantiVAR_061087338M → V.Corresponds to variant rs35456905dbSNPEnsembl.1
Natural variantiVAR_012152399R → Q.1 PublicationCorresponds to variant rs200372110dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96824 mRNA. Translation: AAA36383.1.
U31342
, U31336, U31337, U31338, U31340, U31341 Genomic DNA. Translation: AAB60431.1.
BT009828 mRNA. Translation: AAP88830.1.
AK315422 mRNA. Translation: BAG37811.1.
CH471177 Genomic DNA. Translation: EAW52411.1.
BC002356 mRNA. Translation: AAH02356.1.
CCDSiCCDS12740.1.
RefSeqiNP_006175.2. NM_006184.5.
XP_016882334.1. XM_017026845.1.
UniGeneiHs.631602.

Genome annotation databases

EnsembliENST00000405315; ENSP00000385923; ENSG00000104805.
ENST00000407032; ENSP00000385211; ENSG00000104805.
GeneIDi4924.
KEGGihsa:4924.
UCSCiuc002plb.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96824 mRNA. Translation: AAA36383.1.
U31342
, U31336, U31337, U31338, U31340, U31341 Genomic DNA. Translation: AAB60431.1.
BT009828 mRNA. Translation: AAP88830.1.
AK315422 mRNA. Translation: BAG37811.1.
CH471177 Genomic DNA. Translation: EAW52411.1.
BC002356 mRNA. Translation: AAH02356.1.
CCDSiCCDS12740.1.
RefSeqiNP_006175.2. NM_006184.5.
XP_016882334.1. XM_017026845.1.
UniGeneiHs.631602.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SNLNMR-A228-326[»]
ProteinModelPortaliQ02818.
SMRiQ02818.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110978. 33 interactors.
IntActiQ02818. 16 interactors.
MINTiMINT-2805455.
STRINGi9606.ENSP00000263273.

Chemistry databases

GuidetoPHARMACOLOGYi2871.

PTM databases

iPTMnetiQ02818.
PhosphoSitePlusiQ02818.

Polymorphism and mutation databases

BioMutaiNUCB1.
DMDMi90110780.

Proteomic databases

EPDiQ02818.
MaxQBiQ02818.
PaxDbiQ02818.
PeptideAtlasiQ02818.
PRIDEiQ02818.

Protocols and materials databases

DNASUi4924.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000405315; ENSP00000385923; ENSG00000104805.
ENST00000407032; ENSP00000385211; ENSG00000104805.
GeneIDi4924.
KEGGihsa:4924.
UCSCiuc002plb.5. human.

Organism-specific databases

CTDi4924.
DisGeNETi4924.
GeneCardsiNUCB1.
HGNCiHGNC:8043. NUCB1.
HPAiHPA008176.
MIMi601323. gene.
neXtProtiNX_Q02818.
OpenTargetsiENSG00000104805.
PharmGKBiPA31825.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3866. Eukaryota.
ENOG410XW7D. LUCA.
GeneTreeiENSGT00390000001927.
HOGENOMiHOG000007216.
HOVERGENiHBG052685.
InParanoidiQ02818.
KOiK20371.
OMAiAKMDAEQ.
OrthoDBiEOG091G0HG8.
PhylomeDBiQ02818.
TreeFamiTF323218.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000104805-MONOMER.

Miscellaneous databases

ChiTaRSiNUCB1. human.
EvolutionaryTraceiQ02818.
GeneWikiiNUCB1.
GenomeRNAii4924.
PROiQ02818.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000104805.
CleanExiHS_NUCB1.
ExpressionAtlasiQ02818. baseline and differential.
GenevisibleiQ02818. HS.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUCB1_HUMAN
AccessioniPrimary (citable) accession number: Q02818
Secondary accession number(s): B2RD64
, Q15838, Q7Z4J7, Q9BUR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: March 7, 2006
Last modified: November 30, 2016
This is version 159 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Discovered as DNA-binding protein in the serum of lupus-prone mice.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.