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Protein

Nucleobindin-1

Gene

NUCB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Major calcium-binding protein of the Golgi. May have a role in calcium homeostasis (By similarity).By similarity

Miscellaneous

Discovered as DNA-binding protein in the serum of lupus-prone mice.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi172 – 218Sequence analysisAdd BLAST47
Calcium bindingi253 – 26411 PublicationAdd BLAST12
Calcium bindingi305 – 31621 PublicationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-HSA-8957275 Post-translational protein phosphorylation
SIGNORiQ02818

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleobindin-1
Alternative name(s):
CALNUC
Gene namesi
Name:NUCB1
Synonyms:NUC
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000104805.15
HGNCiHGNC:8043 NUCB1
MIMi601323 gene
neXtProtiNX_Q02818

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi4924
OpenTargetsiENSG00000104805
PharmGKBiPA31825

Chemistry databases

GuidetoPHARMACOLOGYi2871

Polymorphism and mutation databases

BioMutaiNUCB1
DMDMi90110780

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000000416227 – 461Nucleobindin-1Add BLAST435

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei86Phosphoserine; by FAM20C1 Publication1
Modified residuei148Phosphothreonine; by FAM20CCombined sources1 Publication1
Modified residuei369Phosphoserine; by FAM20C1 Publication1

Post-translational modificationi

O-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ02818
MaxQBiQ02818
PaxDbiQ02818
PeptideAtlasiQ02818
PRIDEiQ02818
ProteomicsDBi58128

PTM databases

iPTMnetiQ02818
PhosphoSitePlusiQ02818

Expressioni

Tissue specificityi

Expressed both in fetal and adult heart, lung, liver, kidney and brain, and in adult skeletal muscle, placenta and pancreas.

Gene expression databases

BgeeiENSG00000104805
CleanExiHS_NUCB1
ExpressionAtlasiQ02818 baseline and differential
GenevisibleiQ02818 HS

Organism-specific databases

HPAiHPA008176

Interactioni

Subunit structurei

Interacts with GNAI2 and GNAI3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Q99IB83EBI-2622179,EBI-6927928From Hepatitis C virus genotype 2a (isolate JFH-1).

GO - Molecular functioni

Protein-protein interaction databases

BioGridi110978, 41 interactors
IntActiQ02818, 17 interactors
MINTiQ02818
STRINGi9606.ENSP00000263273

Structurei

Secondary structure

1461
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi245 – 252Combined sources8
Beta strandi258 – 261Combined sources4
Helixi262 – 274Combined sources13
Helixi285 – 288Combined sources4
Helixi291 – 303Combined sources13
Beta strandi309 – 313Combined sources5
Helixi314 – 322Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SNLNMR-A228-326[»]
ProteinModelPortaliQ02818
SMRiQ02818
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ02818

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini240 – 275EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini292 – 327EF-hand 2PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni42 – 51O-glycosylated at one site10
Regioni228 – 321Binds to GNAI2 and GNAI3By similarityAdd BLAST94

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili150 – 218Sequence analysisAdd BLAST69
Coiled coili341 – 407Sequence analysisAdd BLAST67

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi401 – 407Poly-Gln7

Domaini

The EF-hand domains are unfolded in the absence of Ca2+ and fold upon Ca2+ addition.

Sequence similaritiesi

Belongs to the nucleobindin family.Curated

Keywords - Domaini

Coiled coil, Repeat, Signal

Phylogenomic databases

eggNOGiKOG3866 Eukaryota
ENOG410XW7D LUCA
GeneTreeiENSGT00390000001927
HOGENOMiHOG000007216
HOVERGENiHBG052685
InParanoidiQ02818
KOiK20371
OMAiAKMDAEQ
OrthoDBiEOG091G0HG8
PhylomeDBiQ02818
TreeFamiTF323218

Family and domain databases

InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF13499 EF-hand_7, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02818-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPSGPRGTL LLLPLLLLLL LRAVLAVPLE RGAPNKEETP ATESPDTGLY
60 70 80 90 100
YHRYLQEVID VLETDGHFRE KLQAANAEDI KSGKLSRELD FVSHHVRTKL
110 120 130 140 150
DELKRQEVSR LRMLLKAKMD AEQDPNVQVD HLNLLKQFEH LDPQNQHTFE
160 170 180 190 200
ARDLELLIQT ATRDLAQYDA AHHEEFKRYE MLKEHERRRY LESLGEEQRK
210 220 230 240 250
EAERKLEEQQ RRHREHPKVN VPGSQAQLKE VWEELDGLDP NRFNPKTFFI
260 270 280 290 300
LHDINSDGVL DEQELEALFT KELEKVYDPK NEEDDMREME EERLRMREHV
310 320 330 340 350
MKNVDTNQDR LVTLEEFLAS TQRKEFGDTG EGWETVEMHP AYTEEELRRF
360 370 380 390 400
EEELAAREAE LNAKAQRLSQ ETEALGRSQG RLEAQKRELQ QAVLHMEQRK
410 420 430 440 450
QQQQQQQGHK APAAHPEGQL KFHPDTDDVP VPAPAGDQKE VDTSEKKLLE
460
RLPEVEVPQH L
Length:461
Mass (Da):53,879
Last modified:March 7, 2006 - v4
Checksum:i913C4B87C2A644C5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti299 – 300HV → QL in AAA36383 (PubMed:1520323).Curated2
Sequence conflicti385Q → K in AAA36383 (PubMed:1520323).Curated1
Sequence conflicti385Q → K in AAB60431 (PubMed:8661046).Curated1
Sequence conflicti390 – 391QQ → LL in AAA36383 (PubMed:1520323).Curated2
Sequence conflicti390 – 391QQ → LL in AAB60431 (PubMed:8661046).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01215113 – 14LP → S1 Publication2
Natural variantiVAR_061087338M → V. Corresponds to variant dbSNP:rs35456905Ensembl.1
Natural variantiVAR_012152399R → Q1 PublicationCorresponds to variant dbSNP:rs200372110Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96824 mRNA Translation: AAA36383.1
U31342
, U31336, U31337, U31338, U31340, U31341 Genomic DNA Translation: AAB60431.1
BT009828 mRNA Translation: AAP88830.1
AK315422 mRNA Translation: BAG37811.1
CH471177 Genomic DNA Translation: EAW52411.1
BC002356 mRNA Translation: AAH02356.1
CCDSiCCDS12740.1
RefSeqiNP_006175.2, NM_006184.5
XP_016882334.1, XM_017026845.1
UniGeneiHs.631602

Genome annotation databases

EnsembliENST00000405315; ENSP00000385923; ENSG00000104805
ENST00000407032; ENSP00000385211; ENSG00000104805
GeneIDi4924
KEGGihsa:4924
UCSCiuc002plb.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiNUCB1_HUMAN
AccessioniPrimary (citable) accession number: Q02818
Secondary accession number(s): B2RD64
, Q15838, Q7Z4J7, Q9BUR1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: March 7, 2006
Last modified: June 20, 2018
This is version 172 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

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