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Q02818

- NUCB1_HUMAN

UniProt

Q02818 - NUCB1_HUMAN

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Protein

Nucleobindin-1

Gene
NUCB1, NUC
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Major calcium-binding protein of the Golgi. May have a role in calcium homeostasis By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi172 – 21847 Reviewed predictionAdd
BLAST
Calcium bindingi253 – 2641211 PublicationAdd
BLAST
Calcium bindingi305 – 3161221 PublicationAdd
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. DNA binding Source: ProtInc
  3. protein binding Source: IntAct
Complete GO annotation...

Keywords - Ligandi

Calcium, DNA-binding, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleobindin-1
Alternative name(s):
CALNUC
Gene namesi
Name:NUCB1
Synonyms:NUC
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:8043. NUCB1.

Subcellular locationi

Golgi apparatuscis-Golgi network membrane; Peripheral membrane protein; Lumenal side By similarity. Cytoplasm By similarity
Note: A small fraction of the protein may be cytoplasmic By similarity.

GO - Cellular componenti

  1. endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB
  2. extracellular space Source: UniProt
  3. extracellular vesicular exosome Source: UniProt
  4. Golgi apparatus Source: UniProtKB-SubCell
  5. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA31825.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626 Reviewed predictionAdd
BLAST
Chaini27 – 461435Nucleobindin-1PRO_0000004162Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei86 – 861Phosphoserine By similarity
Modified residuei369 – 3691Phosphoserine By similarity

Post-translational modificationi

O-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ02818.
PaxDbiQ02818.
PRIDEiQ02818.

PTM databases

PhosphoSiteiQ02818.

Expressioni

Tissue specificityi

Expressed both in fetal and adult heart, lung, liver, kidney and brain, and in adult skeletal muscle, placenta and pancreas.

Gene expression databases

ArrayExpressiQ02818.
BgeeiQ02818.
CleanExiHS_NUCB1.
GenevestigatoriQ02818.

Organism-specific databases

HPAiHPA008176.

Interactioni

Subunit structurei

Interacts with GNAI2 and GNAI3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Q99IB83EBI-2622179,EBI-6927928From a different organism.

Protein-protein interaction databases

BioGridi110978. 20 interactions.
IntActiQ02818. 15 interactions.
MINTiMINT-2805455.
STRINGi9606.ENSP00000263273.

Structurei

Secondary structure

1
461
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi245 – 2528
Beta strandi258 – 2614
Helixi262 – 27413
Helixi285 – 2884
Helixi291 – 30313
Beta strandi309 – 3135
Helixi314 – 3229

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SNLNMR-A228-326[»]
ProteinModelPortaliQ02818.
SMRiQ02818. Positions 228-326.

Miscellaneous databases

EvolutionaryTraceiQ02818.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini240 – 27536EF-hand 1Add
BLAST
Domaini292 – 32736EF-hand 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni42 – 5110O-glycosylated at one site
Regioni228 – 32194Binds to GNAI2 and GNAI3 By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili150 – 21869 Reviewed predictionAdd
BLAST
Coiled coili341 – 40767 Reviewed predictionAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi401 – 4077Poly-Gln

Domaini

The EF-hand domains are unfolded in the absence of Ca2+ and fold upon Ca2+ addition.

Sequence similaritiesi

Belongs to the nucleobindin family.
Contains 2 EF-hand domains.

Keywords - Domaini

Coiled coil, Repeat, Signal

Phylogenomic databases

eggNOGiNOG262100.
HOGENOMiHOG000007216.
HOVERGENiHBG052685.
InParanoidiQ02818.
OMAiMDAEQEP.
OrthoDBiEOG7WQ7SZ.
PhylomeDBiQ02818.
TreeFamiTF323218.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 1 hit.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02818-1 [UniParc]FASTAAdd to Basket

« Hide

MPPSGPRGTL LLLPLLLLLL LRAVLAVPLE RGAPNKEETP ATESPDTGLY    50
YHRYLQEVID VLETDGHFRE KLQAANAEDI KSGKLSRELD FVSHHVRTKL 100
DELKRQEVSR LRMLLKAKMD AEQDPNVQVD HLNLLKQFEH LDPQNQHTFE 150
ARDLELLIQT ATRDLAQYDA AHHEEFKRYE MLKEHERRRY LESLGEEQRK 200
EAERKLEEQQ RRHREHPKVN VPGSQAQLKE VWEELDGLDP NRFNPKTFFI 250
LHDINSDGVL DEQELEALFT KELEKVYDPK NEEDDMREME EERLRMREHV 300
MKNVDTNQDR LVTLEEFLAS TQRKEFGDTG EGWETVEMHP AYTEEELRRF 350
EEELAAREAE LNAKAQRLSQ ETEALGRSQG RLEAQKRELQ QAVLHMEQRK 400
QQQQQQQGHK APAAHPEGQL KFHPDTDDVP VPAPAGDQKE VDTSEKKLLE 450
RLPEVEVPQH L 461
Length:461
Mass (Da):53,879
Last modified:March 7, 2006 - v4
Checksum:i913C4B87C2A644C5
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti13 – 142LP → S.
VAR_012151
Natural varianti338 – 3381M → V.
Corresponds to variant rs35456905 [ dbSNP | Ensembl ].
VAR_061087
Natural varianti399 – 3991R → Q.1 Publication
VAR_012152

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti299 – 3002HV → QL in AAA36383. 1 Publication
Sequence conflicti385 – 3851Q → K in AAA36383. 1 Publication
Sequence conflicti385 – 3851Q → K in AAB60431. 1 Publication
Sequence conflicti390 – 3912QQ → LL in AAA36383. 1 Publication
Sequence conflicti390 – 3912QQ → LL in AAB60431. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M96824 mRNA. Translation: AAA36383.1.
U31342
, U31336, U31337, U31338, U31340, U31341 Genomic DNA. Translation: AAB60431.1.
BT009828 mRNA. Translation: AAP88830.1.
AK315422 mRNA. Translation: BAG37811.1.
CH471177 Genomic DNA. Translation: EAW52411.1.
BC002356 mRNA. Translation: AAH02356.1.
CCDSiCCDS12740.1.
RefSeqiNP_006175.2. NM_006184.5.
UniGeneiHs.631602.

Genome annotation databases

EnsembliENST00000263273; ENSP00000263273; ENSG00000104805.
ENST00000405315; ENSP00000385923; ENSG00000104805.
ENST00000407032; ENSP00000385211; ENSG00000104805.
GeneIDi4924.
KEGGihsa:4924.
UCSCiuc002pla.3. human.

Polymorphism databases

DMDMi90110780.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M96824 mRNA. Translation: AAA36383.1 .
U31342
, U31336 , U31337 , U31338 , U31340 , U31341 Genomic DNA. Translation: AAB60431.1 .
BT009828 mRNA. Translation: AAP88830.1 .
AK315422 mRNA. Translation: BAG37811.1 .
CH471177 Genomic DNA. Translation: EAW52411.1 .
BC002356 mRNA. Translation: AAH02356.1 .
CCDSi CCDS12740.1.
RefSeqi NP_006175.2. NM_006184.5.
UniGenei Hs.631602.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1SNL NMR - A 228-326 [» ]
ProteinModelPortali Q02818.
SMRi Q02818. Positions 228-326.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 110978. 20 interactions.
IntActi Q02818. 15 interactions.
MINTi MINT-2805455.
STRINGi 9606.ENSP00000263273.

PTM databases

PhosphoSitei Q02818.

Polymorphism databases

DMDMi 90110780.

Proteomic databases

MaxQBi Q02818.
PaxDbi Q02818.
PRIDEi Q02818.

Protocols and materials databases

DNASUi 4924.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000263273 ; ENSP00000263273 ; ENSG00000104805 .
ENST00000405315 ; ENSP00000385923 ; ENSG00000104805 .
ENST00000407032 ; ENSP00000385211 ; ENSG00000104805 .
GeneIDi 4924.
KEGGi hsa:4924.
UCSCi uc002pla.3. human.

Organism-specific databases

CTDi 4924.
GeneCardsi GC19P049403.
HGNCi HGNC:8043. NUCB1.
HPAi HPA008176.
MIMi 601323. gene.
neXtProti NX_Q02818.
PharmGKBi PA31825.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG262100.
HOGENOMi HOG000007216.
HOVERGENi HBG052685.
InParanoidi Q02818.
OMAi MDAEQEP.
OrthoDBi EOG7WQ7SZ.
PhylomeDBi Q02818.
TreeFami TF323218.

Miscellaneous databases

ChiTaRSi NUCB1. human.
EvolutionaryTracei Q02818.
GeneWikii NUCB1.
GenomeRNAii 4924.
NextBioi 18963.
PROi Q02818.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q02818.
Bgeei Q02818.
CleanExi HS_NUCB1.
Genevestigatori Q02818.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
InterProi IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view ]
Pfami PF13499. EF-hand_7. 1 hit.
[Graphical view ]
PROSITEi PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of nucleobindin, a novel DNA-binding protein that contains both a signal peptide and a leucine zipper structure."
    Miura K., Titani K., Kurosawa Y., Kanai Y.
    Biochem. Biophys. Res. Commun. 187:375-380(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Organization of the human gene for nucleobindin (NUC) and its chromosomal assignment to 19q13.2-q13.4."
    Miura K., Hirai M., Kanai Y., Kurosawa Y.
    Genomics 34:181-186(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS 13-LEU-PRO-14 DELINS SER AND GLN-399, SEQUENCE REVISION TO 299-300.
    Tissue: Placenta.
  3. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Umbilical cord blood.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  7. "Interaction of the protein nucleobindin with G alpha i2, as revealed by the yeast two-hybrid system."
    Mochizuki N., Hibi M., Kanai Y., Insel P.A.
    FEBS Lett. 373:155-158(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, INTERACTION WITH GNAI2.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins."
    Halim A., Ruetschi U., Larson G., Nilsson J.
    J. Proteome Res. 12:573-584(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION, IDENTIFICATION BY MASS SPECTROMETRY.
  10. "Structural studies on the Ca2+-binding domain of human nucleobindin (Calnuc)."
    de Alba E., Tjandra N.
    Biochemistry 43:10039-10049(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 228-326, CALCIUM-BINDING.

Entry informationi

Entry nameiNUCB1_HUMAN
AccessioniPrimary (citable) accession number: Q02818
Secondary accession number(s): B2RD64
, Q15838, Q7Z4J7, Q9BUR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: March 7, 2006
Last modified: September 3, 2014
This is version 135 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Discovered as DNA-binding protein in the serum of lupus-prone mice.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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