Protein ROD1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q02805 (ROD1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 99. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein ROD1

Alternative name(s):
Resistance to o-dinitrobenzene protein 1

Gene names

Name:	ROD1
Ordered Locus Names:	YOR018W
ORF Names:	OR26.08

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	837 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates resistance to o-dinitrobenzene, calcium and zinc. Ref.1 Ref.4
Subunit structure	Interacts with RSP5 via its 2 PY-motifs. Ref.4
Subcellular location	Membrane; Peripheral membrane protein. Note: Membrane-associated or associated to a complex that cofractionates with plasma membrane. Ref.1
Domain	The PY-motifs are required for the interaction with RSP5 ubiquitin-ligase and important for resistance to o-dinitrobenzene. Ref.4
Miscellaneous	Present with 386 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the arrestin family.

Ontologies

Keywords
Cellular component	Membrane
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to drug Inferred from mutant phenotype Ref.4. Source: SGD ubiquitin-dependent endocytosis Inferred from sequence alignment PubMed 18976803. Source: SGD
Cellular_component	plasma membrane Inferred from direct assay Ref.1. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RSP5	P39940	3	EBI-15679,EBI-16219

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 837

837

Protein ROD1

PRO_0000097396

Regions

Motif

487 – 490

PY-motif

Motif

656 – 659

PY-motif

Compositional bias

3 – 6

Poly-Ser

Compositional bias

524 – 601

Asn-rich

Compositional bias

684 – 815

132

Ser-rich

Amino acid modifications

Modified residue

123

Phosphoserine Ref.9

Modified residue

124

Phosphoserine Ref.9

Modified residue

125

Phosphothreonine Ref.6

Modified residue

138

Phosphoserine Ref.9

Modified residue

139

Phosphothreonine Ref.9

Modified residue

141

Phosphoserine Ref.7 Ref.9

Modified residue

436

Phosphoserine Ref.7 Ref.9

Modified residue

536

Phosphoserine Ref.9

Modified residue

602

Phosphoserine Ref.8 Ref.9

Modified residue

604

Phosphoserine Ref.9

Modified residue

606

Phosphothreonine Ref.9

Modified residue

626

Phosphoserine Ref.8 Ref.9

Modified residue

684

Phosphoserine Ref.9

Modified residue

685

Phosphothreonine Ref.9

Modified residue

720

Phosphoserine Ref.7 Ref.9

Modified residue

723

Phosphoserine Ref.9

Modified residue

725

Phosphoserine Ref.9

Modified residue

813

Phosphoserine Ref.9

Modified residue

815

Phosphoserine Ref.9

Experimental info

Mutagenesis

487 – 488

PP → QA: Reduced binding to RSP5. Ref.4

Mutagenesis

656 – 657

PP → QA: Reduced binding to RSP5. Ref.4

Sequence conflict

618

Y → D in AAB03678. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q02805 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: FD9A64174B1DC443
Show »

FASTA

837

92,349

        10         20         30         40         50         60 
MFSSSSRPSK EPLLFDIRLR NLDNDVLLIK GPPDEASSVL LSGTIVLSIT EPIQIKSLAL 

        70         80         90        100        110        120 
RLFGRLRLNI PTVLQTVHGP HKRYSKFERN IYSHFWDDFN IKSYFQNLYD NHNNGKITIS 

       130        140        150        160        170        180 
SKSSTNLAAL PKRKRALSTA SLISSNGQTS ASKNYHTLVK GNYEFPFSAI IPGSLVESVE 

       190        200        210        220        230        240 
GLPNAAVTYA LEATIERPKQ PDLICKKHLR VIRTLAIDAV ELSETVSVDN SWPEKVDYTI 

       250        260        270        280        290        300 
SIPTKAIAIG SSTMINILIV PILKGLKLGP VRISLVENSQ YCGSYGGVIN QERMVAKLKL 

       310        320        330        340        350        360 
KDPLKHVAQI KKKRSLNEAA DEGVDTDTGE FQDKWEVRAL LNIPASLTKC SQDCRILSNI 

       370        380        390        400        410        420 
KVRHKIKFTI SLLNPDGHIS ELRAALPVQL FISPFVPVNV KTSDVIERTL KTFGPSYQVT 

       430        440        450        460        470        480 
SQHDNSFSSK NFVDDSEEDV IFQRSASALQ LSSMPTIVSG STLNINSTDA EATAVADTTM 

       490        500        510        520        530        540 
VTSLMVPPNY GNHVYDRVYG EVTNEDETSA SASSSAVESQ AIHNIQNLYI SDSNNSNNPI 

       550        560        570        580        590        600 
LAPNPQIKIE DDSLNNCDSR GDSVNNSNLN LVNSNLTISE NWNNNSPSAN RYNNIINAGL 

       610        620        630        640        650        660 
NSPSLTPSFA HLSRRNSYSR QTSSTSLKND LELTDLSRVP SYDKAMKSDM IGEDLPPAYP 

       670        680        690        700        710        720 
EEELGVQENK KIELERPQIL HHKSTSSLLP LPGSSKSSNN LKRSSSRTHL SHSPLPRNNS 

       730        740        750        760        770        780 
GSSVSLQQLA RNNTDSSFNL NLSFTSAKSS TGSRHFPFNM TTSFTSNSSS KNNSHFDKTD 

       790        800        810        820        830 
STSDANKPRE EENYTSATHN RRSRSSSVRS NNSNSPLRQG TGSFANLMEM FTKRDRS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ROD1, a novel gene conferring multiple resistance phenotypes in Saccharomyces cerevisiae." Wu A.L., Hallstrom T.C., Moye-Rowley W.S. J. Biol. Chem. 271:2914-2920(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"PY motifs of Rod1 are required for binding to Rsp5 and for drug resistance." Andoh T., Hirata Y., Kikuchi A. FEBS Lett. 525:131-134(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DOMAIN, INTERACTION WITH RSP5, MUTAGENESIS OF 487-PRO-PRO-488 AND 656-PRO-PRO-657.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-125, MASS SPECTROMETRY. Strain: YAL6B.
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-141; SER-436 AND SER-720, MASS SPECTROMETRY. Strain: ADR376.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-602 AND SER-626, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-123; SER-124; SER-138; THR-139; SER-141; SER-436; SER-536; SER-602; SER-604; THR-606; SER-626; SER-684; THR-685; SER-720; SER-723; SER-725; SER-813 AND SER-815, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U40561 Genomic DNA. Translation: AAB03678.1. X87331 Genomic DNA. Translation: CAA60767.1. Z74926 Genomic DNA. Translation: CAA99208.1. BK006948 Genomic DNA. Translation: DAA10800.1.
PIR	S54624.
RefSeq	NP_014661.1. NM_001183437.1.
3D structure databases
ProteinModelPortal	Q02805.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4189N.
IntAct	Q02805. 5 interactions.
MINT	MINT-523247.
STRING	4932.YOR018W.
Proteomic databases
PaxDb	Q02805.
PeptideAtlas	Q02805.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR018W; YOR018W; YOR018W.
GeneID	854183.
KEGG	sce:YOR018W.
Organism-specific databases
CYGD	YOR018w.
SGD	S000005544. ROD1.
Phylogenomic databases
eggNOG	NOG275030.
GeneTree	ENSGT00530000067129.
HOGENOM	HOG000248143.
OMA	DNTWPKK.
OrthoDB	EOG4KSST1.
Gene expression databases
Genevestigator	Q02805.
GermOnline	YOR018W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011021. Arrestin-like_N. IPR011022. Arrestin_C-like. [Graphical view]
Pfam	PF02752. Arrestin_C. 1 hit. PF00339. Arrestin_N. 1 hit. [Graphical view]
SMART	SM01017. Arrestin_C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	975990.

Entry information

Entry name

ROD1_YEAST

Accession

Primary (citable) accession number: Q02805
Secondary accession number(s): D6W284, Q12475

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents