Q02804 (ARL3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ADP-ribosylation factor-like protein 3

Alternative name(s):
Arf-like GTPase 3

Gene names

Name:	ARL3
Synonyms:	ARP1
Ordered Locus Names:	YPL051W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the targeting of ARL1 to the Golgi. Can bind and hydrolyze GTP. Ref.5 Ref.6
Subunit structure	Interacts with SYS1 and SLO1. Ref.6 Ref.8 Ref.9
Subcellular location	Golgi apparatus.
Miscellaneous	Present with 1920 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the small GTPase superfamily. Arf family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Golgi apparatus
Ligand	GTP-binding Nucleotide-binding
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Golgi to plasma membrane protein transport Inferred from mutant phenotype. Source: SGD protein localization to organelle Inferred from mutant phenotype Ref.6. Source: SGD small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from direct assay Ref.8Ref.9. Source: SGD nuclear outer membrane-endoplasmic reticulum membrane network Inferred from direct assay Ref.1. Source: SGD
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from direct assay Ref.1. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 198	198	ADP-ribosylation factor-like protein 3		PRO_0000207422
Regions
	Nucleotide binding	24 – 31	8	GTP By similarity
	Nucleotide binding	74 – 78	5	GTP By similarity
	Nucleotide binding	133 – 136	4	GTP By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.8 Ref.9

Sequences

	Sequence		Length	Mass (Da)
	Q02804 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 46E8CFEB1931A220	FASTA	198	22,782
	10 20 30 40 50 60 MFHLVKGLYN NWNKKEQYSI LILGLDNAGK TTFLETLKKE YSLAFKALEK IQPTVGQNVA 70 80 90 100 110 120 TIPVDSKQIL KFWDVGGQES LRSMWSEYYS LCHGIIFIVD SSDRERLDEC STTLQSVVMD 130 140 150 160 170 180 EEIEGVPILM LANKQDRQDR MEVQDIKEVF NKIAEHISAR DSRVLPISAL TGEGVKDAIE 190 WMIVRLERNK KSRPPIYK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a novel ADP-ribosylation factor-like protein (yARL3) in Saccharomyces cerevisiae." Huang C.F., Buu L.M., Yu W.L., Lee F.-J.S. J. Biol. Chem. 274:3819-3827(1999) [PubMed: 9920936] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Golgi recruitment of GRIP domain proteins by Arf-like GTPase 1 is regulated by Arf-like GTPase 3." Setty S.R.G., Shin M.E., Yoshino A., Marks M.S., Burd C.G. Curr. Biol. 13:401-404(2003) [PubMed: 12620188] [Abstract] Cited for: FUNCTION.
[6]	"The ARF-like GTPases Arl1p and Arl3p act in a pathway that interacts with vesicle-tethering factors at the Golgi apparatus." Panic B., Whyte J.R.C., Munro S. Curr. Biol. 13:405-410(2003) [PubMed: 12620189] [Abstract] Cited for: FUNCTION, INTERACTION WITH SLO1.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Targeting of the Arf-like GTPase Arl3p to the Golgi requires N-terminal acetylation and the membrane protein Sys1p." Behnia R., Panic B., Whyte J.R.C., Munro S. Nat. Cell Biol. 6:405-413(2004) [PubMed: 15077113] [Abstract] Cited for: ACETYLATION AT MET-1, INTERACTION WITH SYS1.
[9]	"Golgi targeting of ARF-like GTPase Arl3p requires its Nalpha-acetylation and the integral membrane protein Sys1p." Setty S.R., Strochlic T.I., Tong A.H., Boone C., Burd C.G. Nat. Cell Biol. 6:414-419(2004) [PubMed: 15077114] [Abstract] Cited for: ACETYLATION AT MET-1, INTERACTION WITH SYS1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF017142 Genomic DNA. Translation: AAD13357.1. U39205 Genomic DNA. Translation: AAB68314.1. AY558323 Genomic DNA. Translation: AAS56649.1. BK006949 Genomic DNA. Translation: DAA11379.1.
PIR	S61091.
RefSeq	NP_015274.1. NM_001183865.1.
3D structure databases
ProteinModelPortal	Q02804.
SMR	Q02804. Positions 5-186.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2122N.
IntAct	Q02804. 6 interactions.
MINT	MINT-393373.
STRING	Q02804.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL051W; YPL051W; YPL051W.
GeneID	856056.
KEGG	sce:YPL051W.
NMPDR	fig|4932.3.peg.6407.
Organism-specific databases
CYGD	YPL051w.
SGD	S000005972. ARL3.
Phylogenomic databases
eggNOG	fuNOG05504.
GeneTree	EFGT00050000002462.
HOGENOM	HBG745225.
OMA	LIGRRDC.
OrthoDB	EOG4Q2HQN.
Gene expression databases
ArrayExpress	Q02804.
Genevestigator	Q02804.
GermOnline	YPL051W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR024156. Small_GTPase_ARF. IPR006689. Small_GTPase_ARF/SAR. [Graphical view]
KO	K07952.
PANTHER	PTHR11711. ARF/SAR. 1 hit.
Pfam	PF00025. Arf. 1 hit. [Graphical view]
PRINTS	PR00328. SAR1GTPBP.
SMART	SM00177. ARF. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. Small_GTP. 1 hit.
PROSITE	PS51417. ARF. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	981023.

Entry information

Entry name

ARL3_YEAST

Accession

Primary (citable) accession number: Q02804
Secondary accession number(s): D6W3W3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program