Transcriptional regulatory protein LGE1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q02796 (LGE1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 96. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Transcriptional regulatory protein LGE1

Alternative name(s):
Large cells protein 1

Gene names

Name:	LGE1
Ordered Locus Names:	YPL055C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required to activate transcription of PDR3, a gene involved in retrograde regulation of multidrug resistance, a phenomenon that takes place in cells that have lost their mitochondrial genome. Also required for ubiquitination of histone H2B to form H2BK123ub1. H2BK123ub1 gives a specific tag for epigenetic transcriptional activation, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. Its precise role in H2BK123ub1 formation however is unclear and is independent of retrograde regulation of multidrug resistance function. Ref.5 Ref.8
Subunit structure	Probably interacts with BRE1. Ref.5 Ref.8
Subcellular location	Nucleus Ref.6.
Miscellaneous	Present with 2060 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Coiled coil
Molecular function	Activator Chromatin regulator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	histone methylation Inferred from mutant phenotype Ref.5. Source: SGD histone ubiquitination Inferred from mutant phenotype Ref.5. Source: SGD premeiotic DNA replication Inferred from mutant phenotype PubMed 18069899. Source: SGD protein monoubiquitination Inferred from mutant phenotype Ref.5. Source: SGD regulation of cell size Inferred from genetic interaction Ref.4. Source: SGD regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from direct assay Ref.6 Ref.8. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 332

332

Transcriptional regulatory protein LGE1

PRO_0000076234

Regions

Coiled coil

284 – 328

Potential

Amino acid modifications

Modified residue

232

Phosphoserine Ref.9

Experimental info

Sequence conflict

R → Q in AAS56364. Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

Q02796 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: C3300ED944003F9D
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FASTA

332

37,309

        10         20         30         40         50         60 
MSGYTGNNYS RYSSTPPRQR GGYHHARRSR GGAGGSYYRG GNASYGARYN SDYEQPPQEG 

        70         80         90        100        110        120 
DLRQTGAYYR NGYTDTRPYY SANSRHYQAQ PSPRYNNGTN SYHLPQRGNS QDTNGRTTSA 

       130        140        150        160        170        180 
SQEDNDEKRV KSRYRNMQAD HPRQQPMSVG STSSRNGSSG NSSTSSTSNG LPPPPSVSSI 

       190        200        210        220        230        240 
TNNRSYHSSA YPYSSSHTYN NYHHRETPPP PPSNGYYAKG YPVHVPENRS NSDGSSSSVV 

       250        260        270        280        290        300 
KKKRILDMKD SPFIYLTDFD KNVKKTNNTE SECEKAREVF KESDSIDSAL EELNLKINSN 

       310        320        330 
ELELRLLNNQ CDKHALNIQL TQEKLDSLLL MQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Systematic identification of pathways that couple cell growth and division in yeast." Jorgensen P., Nishikawa J.L., Breitkreutz B.-J., Tyers M. Science 297:395-400(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[5]	"A conserved RING finger protein required for histone H2B monoubiquitination and cell size control." Hwang W.W., Venkatasubrahmanyam S., Ianculescu A.G., Tong A., Boone C., Madhani H.D. Mol. Cell 11:261-266(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH BRE1.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Transcriptional regulation by Lge1p requires a function independent of its role in histone H2B ubiquitination." Zhang X., Kolaczkowska A., Devaux F., Panwar S.L., Hallstrom T.C., Jacq C., Moye-Rowley W.S. J. Biol. Chem. 280:2759-2770(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH BRE1.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U39205 Genomic DNA. Translation: AAB68310.1. AY558038 Genomic DNA. Translation: AAS56364.1. BK006949 Genomic DNA. Translation: DAA11375.1.
PIR	S60935.
RefSeq	NP_015270.1. NM_001183869.1.
3D structure databases
ProteinModelPortal	Q02796.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6470N.
IntAct	Q02796. 80 interactions.
MINT	MINT-597163.
STRING	4932.YPL055C.
Proteomic databases
PaxDb	Q02796.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL055C; YPL055C; YPL055C.
GeneID	856052.
KEGG	sce:YPL055C.
Organism-specific databases
CYGD	YPL055c.
SGD	S000005976. LGE1.
Phylogenomic databases
eggNOG	NOG68967.
OMA	NTESECE.
OrthoDB	EOG4VMJRB.
Gene expression databases
Genevestigator	Q02796.
GermOnline	YPL055C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	981011.

Entry information

Entry name

LGE1_YEAST

Accession

Primary (citable) accession number: Q02796
Secondary accession number(s): D6W3V9, Q6Q5I8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents