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Q02795

- OSTD_YEAST

UniProt

Q02795 - OSTD_YEAST

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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1

Gene

SWP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.

Catalytic activityi

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathwayi

GO - Molecular functioni

  1. transferase activity, transferring glycosyl groups Source: UniProtKB-KW

GO - Biological processi

  1. protein N-linked glycosylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:YMR149W-MONOMER.
BRENDAi2.4.1.119. 984.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1 (EC:2.4.99.18)
Short name:
Oligosaccharyl transferase subunit SWP1
Alternative name(s):
Oligosaccharyl transferase subunit delta
Gene namesi
Name:SWP1
Ordered Locus Names:YMR149W
ORF Names:YM9375.19
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR149w.
SGDiS000004757. SWP1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 194175LumenalSequence AnalysisAdd
BLAST
Transmembranei195 – 21521HelicalSequence AnalysisAdd
BLAST
Topological domaini216 – 22813CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei229 – 24921HelicalSequence AnalysisAdd
BLAST
Topological domaini250 – 2523LumenalSequence Analysis
Transmembranei253 – 27321HelicalSequence AnalysisAdd
BLAST
Topological domaini274 – 28613CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. oligosaccharyltransferase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 19191 PublicationAdd
BLAST
Chaini20 – 286267Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1PRO_0000021962Add
BLAST

Proteomic databases

MaxQBiQ02795.
PaxDbiQ02795.
PeptideAtlasiQ02795.

Expressioni

Gene expression databases

GenevestigatoriQ02795.

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex, which appears to exist in two assemblies comprising OST1, OST2, OST4, OST5, STT3, SWP1, WPB1, and either OST3 or OST6. WBP1, SWP1 and OST2 probably form a subcomplex. May interact directly with OST1, OST2, OST4, STT3 and WBP1. Interacts with SEC61 and SSS1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SEC61P329153EBI-12666,EBI-16400
SSS1P351792EBI-12666,EBI-16406
WBP1P337674EBI-12666,EBI-12658

Protein-protein interaction databases

BioGridi35325. 222 interactions.
DIPiDIP-2460N.
IntActiQ02795. 37 interactions.
MINTiMINT-522209.
STRINGi4932.YMR149W.

Structurei

3D structure databases

ProteinModelPortaliQ02795.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SWP1 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG306613.
HOGENOMiHOG000115299.
InParanoidiQ02795.
KOiK12667.
OMAiLEMAFEP.
OrthoDBiEOG7JQC05.

Family and domain databases

InterProiIPR008814. Swp1.
[Graphical view]
PANTHERiPTHR12640:SF0. PTHR12640:SF0. 1 hit.
PfamiPF05817. Ribophorin_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02795-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQFFKTLAAL VSCISFVLAY VAQDVHVSFP STAGKSRVMI GKVEPRIGID
60 70 80 90 100
ETVPTTITVE DPNEVIQVNF AIESTNKPFQ NTLLIGLPNK NLEMAFEPEI
110 120 130 140 150
KDNGKLSMYK YRIDLAKLDA ALLQEASRSP EPIKATLILA SSTAKPKENL
160 170 180 190 200
FREILQLNLN FDVDHSDSSL VDKFGIKPEI HHIFHAEPKR VAKPIAVIFV
210 220 230 240 250
LIIFITILSL IVTWLNSCAA AFNNIPTGVT AVYFLGFIAT IVGFEVIFAR
260 270 280
YYLGTSIFET LFSSLYLGAP GLLTSTKFLR SFGQTI
Length:286
Mass (Da):31,653
Last modified:July 1, 1993 - v1
Checksum:i53D15B8857A534E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67705 Genomic DNA. Translation: CAA47943.1.
Z47071 Genomic DNA. Translation: CAA87364.1.
AY558418 Genomic DNA. Translation: AAS56744.1.
BK006946 Genomic DNA. Translation: DAA10045.1.
PIRiS28416.
RefSeqiNP_013869.1. NM_001182651.1.

Genome annotation databases

EnsemblFungiiYMR149W; YMR149W; YMR149W.
GeneIDi855180.
KEGGisce:YMR149W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67705 Genomic DNA. Translation: CAA47943.1 .
Z47071 Genomic DNA. Translation: CAA87364.1 .
AY558418 Genomic DNA. Translation: AAS56744.1 .
BK006946 Genomic DNA. Translation: DAA10045.1 .
PIRi S28416.
RefSeqi NP_013869.1. NM_001182651.1.

3D structure databases

ProteinModelPortali Q02795.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35325. 222 interactions.
DIPi DIP-2460N.
IntActi Q02795. 37 interactions.
MINTi MINT-522209.
STRINGi 4932.YMR149W.

Proteomic databases

MaxQBi Q02795.
PaxDbi Q02795.
PeptideAtlasi Q02795.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR149W ; YMR149W ; YMR149W .
GeneIDi 855180.
KEGGi sce:YMR149W.

Organism-specific databases

CYGDi YMR149w.
SGDi S000004757. SWP1.

Phylogenomic databases

eggNOGi NOG306613.
HOGENOMi HOG000115299.
InParanoidi Q02795.
KOi K12667.
OMAi LEMAFEP.
OrthoDBi EOG7JQC05.

Enzyme and pathway databases

UniPathwayi UPA00378 .
BioCyci YEAST:YMR149W-MONOMER.
BRENDAi 2.4.1.119. 984.

Miscellaneous databases

NextBioi 978635.

Gene expression databases

Genevestigatori Q02795.

Family and domain databases

InterProi IPR008814. Swp1.
[Graphical view ]
PANTHERi PTHR12640:SF0. PTHR12640:SF0. 1 hit.
Pfami PF05817. Ribophorin_II. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Yeast Wbp1p and Swp1p form a protein complex essential for oligosaccharyl transferase activity."
    Te Heesen S., Knauer R., Lehle L., Aebi M.
    EMBO J. 12:279-284(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "The Saccharomyces cerevisiae oligosaccharyltransferase is a protein complex composed of Wbp1p, Swp1p, and four additional polypeptides."
    Kelleher D.J., Gilmore R.
    J. Biol. Chem. 269:12908-12917(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 20-35, IDENTIFICATION IN THE OLIGOSACCHARYL TRANSFERASE COMPLEX.
  6. "The highly conserved Stt3 protein is a subunit of the yeast oligosaccharyltransferase and forms a subcomplex with Ost3p and Ost4p."
    Karaoglu D., Kelleher D.J., Gilmore R.
    J. Biol. Chem. 272:32513-32520(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE OLIGOSACCHARYL TRANSFERASE COMPLEX.
  7. "The oligosaccharyltransferase complex from yeast."
    Knauer R., Lehle L.
    Biochim. Biophys. Acta 1426:259-273(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW ON OLIGOSACCHARYL TRANSFERASE.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Yeast oligosaccharyltransferase consists of two functionally distinct sub-complexes, specified by either the Ost3p or Ost6p subunit."
    Schwarz M., Knauer R., Lehle L.
    FEBS Lett. 579:6564-6568(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
  10. "The 3.4-kDa Ost4 protein is required for the assembly of two distinct oligosaccharyltransferase complexes in yeast."
    Spirig U., Bodmer D., Wacker M., Burda P., Aebi M.
    Glycobiology 15:1396-1406(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
  11. "Two oligosaccharyl transferase complexes exist in yeast and associate with two different translocons."
    Yan A., Lennarz W.J.
    Glycobiology 15:1407-1415(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
  12. "Subunits of the translocon interact with components of the oligosaccharyl transferase complex."
    Chavan M., Yan A., Lennarz W.J.
    J. Biol. Chem. 280:22917-22924(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SSS1 AND SEC61.
  13. "Studies of yeast oligosaccharyl transferase subunits using the split-ubiquitin system: topological features and in vivo interactions."
    Yan A., Wu E., Lennarz W.J.
    Proc. Natl. Acad. Sci. U.S.A. 102:7121-7126(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY, INTERACTION WITH OST1; OST2; OST4; STT3 AND WBP1.

Entry informationi

Entry nameiOSTD_YEAST
AccessioniPrimary (citable) accession number: Q02795
Secondary accession number(s): D6VZX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: October 29, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8450 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3