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Q02795 (OSTD_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1

Short name=Oligosaccharyl transferase subunit SWP1
EC=2.4.99.18
Alternative name(s):
Oligosaccharyl transferase subunit delta
Gene names
Name:SWP1
Ordered Locus Names:YMR149W
ORF Names:YM9375.19
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length286 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.

Catalytic activity

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathway

Protein modification; protein glycosylation.

Subunit structure

Component of the oligosaccharyltransferase (OST) complex, which appears to exist in two assemblies comprising OST1, OST2, OST4, OST5, STT3, SWP1, WPB1, and either OST3 or OST6. WBP1, SWP1 and OST2 probably form a subcomplex. May interact directly with OST1, OST2, OST4, STT3 and WBP1. Interacts with SEC61 and SSS1. Ref.5 Ref.6 Ref.12 Ref.13

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Miscellaneous

Present with 8450 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the SWP1 family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Ref.5
Chain20 – 286267Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1
PRO_0000021962

Regions

Topological domain20 – 194175Lumenal Potential
Transmembrane195 – 21521Helical; Potential
Topological domain216 – 22813Cytoplasmic Potential
Transmembrane229 – 24921Helical; Potential
Topological domain250 – 2523Lumenal Potential
Transmembrane253 – 27321Helical; Potential
Topological domain274 – 28613Cytoplasmic Potential

Sequences

Sequence LengthMass (Da)Tools
Q02795 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: 53D15B8857A534E5

FASTA28631,653
        10         20         30         40         50         60 
MQFFKTLAAL VSCISFVLAY VAQDVHVSFP STAGKSRVMI GKVEPRIGID ETVPTTITVE 

        70         80         90        100        110        120 
DPNEVIQVNF AIESTNKPFQ NTLLIGLPNK NLEMAFEPEI KDNGKLSMYK YRIDLAKLDA 

       130        140        150        160        170        180 
ALLQEASRSP EPIKATLILA SSTAKPKENL FREILQLNLN FDVDHSDSSL VDKFGIKPEI 

       190        200        210        220        230        240 
HHIFHAEPKR VAKPIAVIFV LIIFITILSL IVTWLNSCAA AFNNIPTGVT AVYFLGFIAT 

       250        260        270        280 
IVGFEVIFAR YYLGTSIFET LFSSLYLGAP GLLTSTKFLR SFGQTI 

« Hide

References

« Hide 'large scale' references
[1]"Yeast Wbp1p and Swp1p form a protein complex essential for oligosaccharyl transferase activity."
Te Heesen S., Knauer R., Lehle L., Aebi M.
EMBO J. 12:279-284(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The Saccharomyces cerevisiae oligosaccharyltransferase is a protein complex composed of Wbp1p, Swp1p, and four additional polypeptides."
Kelleher D.J., Gilmore R.
J. Biol. Chem. 269:12908-12917(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 20-35, IDENTIFICATION IN THE OLIGOSACCHARYL TRANSFERASE COMPLEX.
[6]"The highly conserved Stt3 protein is a subunit of the yeast oligosaccharyltransferase and forms a subcomplex with Ost3p and Ost4p."
Karaoglu D., Kelleher D.J., Gilmore R.
J. Biol. Chem. 272:32513-32520(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE OLIGOSACCHARYL TRANSFERASE COMPLEX.
[7]"The oligosaccharyltransferase complex from yeast."
Knauer R., Lehle L.
Biochim. Biophys. Acta 1426:259-273(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW ON OLIGOSACCHARYL TRANSFERASE.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Yeast oligosaccharyltransferase consists of two functionally distinct sub-complexes, specified by either the Ost3p or Ost6p subunit."
Schwarz M., Knauer R., Lehle L.
FEBS Lett. 579:6564-6568(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
[10]"The 3.4-kDa Ost4 protein is required for the assembly of two distinct oligosaccharyltransferase complexes in yeast."
Spirig U., Bodmer D., Wacker M., Burda P., Aebi M.
Glycobiology 15:1396-1406(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
[11]"Two oligosaccharyl transferase complexes exist in yeast and associate with two different translocons."
Yan A., Lennarz W.J.
Glycobiology 15:1407-1415(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
[12]"Subunits of the translocon interact with components of the oligosaccharyl transferase complex."
Chavan M., Yan A., Lennarz W.J.
J. Biol. Chem. 280:22917-22924(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SSS1 AND SEC61.
[13]"Studies of yeast oligosaccharyl transferase subunits using the split-ubiquitin system: topological features and in vivo interactions."
Yan A., Wu E., Lennarz W.J.
Proc. Natl. Acad. Sci. U.S.A. 102:7121-7126(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY, INTERACTION WITH OST1; OST2; OST4; STT3 AND WBP1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X67705 Genomic DNA. Translation: CAA47943.1.
Z47071 Genomic DNA. Translation: CAA87364.1.
AY558418 Genomic DNA. Translation: AAS56744.1.
BK006946 Genomic DNA. Translation: DAA10045.1.
PIRS28416.
RefSeqNP_013869.1. NM_001182651.1.

3D structure databases

ProteinModelPortalQ02795.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35325. 222 interactions.
DIPDIP-2460N.
IntActQ02795. 37 interactions.
MINTMINT-522209.
STRING4932.YMR149W.

Proteomic databases

MaxQBQ02795.
PaxDbQ02795.
PeptideAtlasQ02795.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR149W; YMR149W; YMR149W.
GeneID855180.
KEGGsce:YMR149W.

Organism-specific databases

CYGDYMR149w.
SGDS000004757. SWP1.

Phylogenomic databases

eggNOGNOG306613.
HOGENOMHOG000115299.
KOK12667.
OMALEMAFEP.
OrthoDBEOG7JQC05.

Enzyme and pathway databases

BioCycYEAST:YMR149W-MONOMER.
BRENDA2.4.1.119. 984.
UniPathwayUPA00378.

Gene expression databases

GenevestigatorQ02795.

Family and domain databases

InterProIPR008814. Swp1.
[Graphical view]
PANTHERPTHR12640:SF0. PTHR12640:SF0. 1 hit.
PfamPF05817. Ribophorin_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio978635.

Entry information

Entry nameOSTD_YEAST
AccessionPrimary (citable) accession number: Q02795
Secondary accession number(s): D6VZX1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: June 11, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways