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Q02795

- OSTD_YEAST

UniProt

Q02795 - OSTD_YEAST

Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1

Gene

SWP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.

    Catalytic activityi

    Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

    Pathwayi

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. transferase activity, transferring glycosyl groups Source: UniProtKB-KW

    GO - Biological processi

    1. protein N-linked glycosylation Source: SGD

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciYEAST:YMR149W-MONOMER.
    BRENDAi2.4.1.119. 984.
    UniPathwayiUPA00378.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1 (EC:2.4.99.18)
    Short name:
    Oligosaccharyl transferase subunit SWP1
    Alternative name(s):
    Oligosaccharyl transferase subunit delta
    Gene namesi
    Name:SWP1
    Ordered Locus Names:YMR149W
    ORF Names:YM9375.19
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR149w.
    SGDiS000004757. SWP1.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. oligosaccharyltransferase complex Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 19191 PublicationAdd
    BLAST
    Chaini20 – 286267Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1PRO_0000021962Add
    BLAST

    Proteomic databases

    MaxQBiQ02795.
    PaxDbiQ02795.
    PeptideAtlasiQ02795.

    Expressioni

    Gene expression databases

    GenevestigatoriQ02795.

    Interactioni

    Subunit structurei

    Component of the oligosaccharyltransferase (OST) complex, which appears to exist in two assemblies comprising OST1, OST2, OST4, OST5, STT3, SWP1, WPB1, and either OST3 or OST6. WBP1, SWP1 and OST2 probably form a subcomplex. May interact directly with OST1, OST2, OST4, STT3 and WBP1. Interacts with SEC61 and SSS1.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SEC61P329153EBI-12666,EBI-16400
    SSS1P351792EBI-12666,EBI-16406
    WBP1P337674EBI-12666,EBI-12658

    Protein-protein interaction databases

    BioGridi35325. 222 interactions.
    DIPiDIP-2460N.
    IntActiQ02795. 37 interactions.
    MINTiMINT-522209.
    STRINGi4932.YMR149W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ02795.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini20 – 194175LumenalSequence AnalysisAdd
    BLAST
    Topological domaini216 – 22813CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini250 – 2523LumenalSequence Analysis
    Topological domaini274 – 28613CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei195 – 21521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei229 – 24921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei253 – 27321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the SWP1 family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG306613.
    HOGENOMiHOG000115299.
    KOiK12667.
    OMAiLEMAFEP.
    OrthoDBiEOG7JQC05.

    Family and domain databases

    InterProiIPR008814. Swp1.
    [Graphical view]
    PANTHERiPTHR12640:SF0. PTHR12640:SF0. 1 hit.
    PfamiPF05817. Ribophorin_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q02795-1 [UniParc]FASTAAdd to Basket

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    MQFFKTLAAL VSCISFVLAY VAQDVHVSFP STAGKSRVMI GKVEPRIGID    50
    ETVPTTITVE DPNEVIQVNF AIESTNKPFQ NTLLIGLPNK NLEMAFEPEI 100
    KDNGKLSMYK YRIDLAKLDA ALLQEASRSP EPIKATLILA SSTAKPKENL 150
    FREILQLNLN FDVDHSDSSL VDKFGIKPEI HHIFHAEPKR VAKPIAVIFV 200
    LIIFITILSL IVTWLNSCAA AFNNIPTGVT AVYFLGFIAT IVGFEVIFAR 250
    YYLGTSIFET LFSSLYLGAP GLLTSTKFLR SFGQTI 286
    Length:286
    Mass (Da):31,653
    Last modified:July 1, 1993 - v1
    Checksum:i53D15B8857A534E5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X67705 Genomic DNA. Translation: CAA47943.1.
    Z47071 Genomic DNA. Translation: CAA87364.1.
    AY558418 Genomic DNA. Translation: AAS56744.1.
    BK006946 Genomic DNA. Translation: DAA10045.1.
    PIRiS28416.
    RefSeqiNP_013869.1. NM_001182651.1.

    Genome annotation databases

    EnsemblFungiiYMR149W; YMR149W; YMR149W.
    GeneIDi855180.
    KEGGisce:YMR149W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X67705 Genomic DNA. Translation: CAA47943.1 .
    Z47071 Genomic DNA. Translation: CAA87364.1 .
    AY558418 Genomic DNA. Translation: AAS56744.1 .
    BK006946 Genomic DNA. Translation: DAA10045.1 .
    PIRi S28416.
    RefSeqi NP_013869.1. NM_001182651.1.

    3D structure databases

    ProteinModelPortali Q02795.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35325. 222 interactions.
    DIPi DIP-2460N.
    IntActi Q02795. 37 interactions.
    MINTi MINT-522209.
    STRINGi 4932.YMR149W.

    Proteomic databases

    MaxQBi Q02795.
    PaxDbi Q02795.
    PeptideAtlasi Q02795.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR149W ; YMR149W ; YMR149W .
    GeneIDi 855180.
    KEGGi sce:YMR149W.

    Organism-specific databases

    CYGDi YMR149w.
    SGDi S000004757. SWP1.

    Phylogenomic databases

    eggNOGi NOG306613.
    HOGENOMi HOG000115299.
    KOi K12667.
    OMAi LEMAFEP.
    OrthoDBi EOG7JQC05.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    BioCyci YEAST:YMR149W-MONOMER.
    BRENDAi 2.4.1.119. 984.

    Miscellaneous databases

    NextBioi 978635.

    Gene expression databases

    Genevestigatori Q02795.

    Family and domain databases

    InterProi IPR008814. Swp1.
    [Graphical view ]
    PANTHERi PTHR12640:SF0. PTHR12640:SF0. 1 hit.
    Pfami PF05817. Ribophorin_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Yeast Wbp1p and Swp1p form a protein complex essential for oligosaccharyl transferase activity."
      Te Heesen S., Knauer R., Lehle L., Aebi M.
      EMBO J. 12:279-284(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "The Saccharomyces cerevisiae oligosaccharyltransferase is a protein complex composed of Wbp1p, Swp1p, and four additional polypeptides."
      Kelleher D.J., Gilmore R.
      J. Biol. Chem. 269:12908-12917(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 20-35, IDENTIFICATION IN THE OLIGOSACCHARYL TRANSFERASE COMPLEX.
    6. "The highly conserved Stt3 protein is a subunit of the yeast oligosaccharyltransferase and forms a subcomplex with Ost3p and Ost4p."
      Karaoglu D., Kelleher D.J., Gilmore R.
      J. Biol. Chem. 272:32513-32520(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE OLIGOSACCHARYL TRANSFERASE COMPLEX.
    7. "The oligosaccharyltransferase complex from yeast."
      Knauer R., Lehle L.
      Biochim. Biophys. Acta 1426:259-273(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW ON OLIGOSACCHARYL TRANSFERASE.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Yeast oligosaccharyltransferase consists of two functionally distinct sub-complexes, specified by either the Ost3p or Ost6p subunit."
      Schwarz M., Knauer R., Lehle L.
      FEBS Lett. 579:6564-6568(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
    10. "The 3.4-kDa Ost4 protein is required for the assembly of two distinct oligosaccharyltransferase complexes in yeast."
      Spirig U., Bodmer D., Wacker M., Burda P., Aebi M.
      Glycobiology 15:1396-1406(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
    11. "Two oligosaccharyl transferase complexes exist in yeast and associate with two different translocons."
      Yan A., Lennarz W.J.
      Glycobiology 15:1407-1415(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
    12. "Subunits of the translocon interact with components of the oligosaccharyl transferase complex."
      Chavan M., Yan A., Lennarz W.J.
      J. Biol. Chem. 280:22917-22924(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SSS1 AND SEC61.
    13. "Studies of yeast oligosaccharyl transferase subunits using the split-ubiquitin system: topological features and in vivo interactions."
      Yan A., Wu E., Lennarz W.J.
      Proc. Natl. Acad. Sci. U.S.A. 102:7121-7126(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY, INTERACTION WITH OST1; OST2; OST4; STT3 AND WBP1.

    Entry informationi

    Entry nameiOSTD_YEAST
    AccessioniPrimary (citable) accession number: Q02795
    Secondary accession number(s): D6VZX1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 8450 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3