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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1

Gene

SWP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.

Catalytic activityi

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • protein N-linked glycosylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:YMR149W-MONOMER.
BRENDAi2.4.99.18. 984.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1 (EC:2.4.99.18)
Short name:
Oligosaccharyl transferase subunit SWP1
Alternative name(s):
Oligosaccharyl transferase subunit delta
Gene namesi
Name:SWP1
Ordered Locus Names:YMR149W
ORF Names:YM9375.19
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR149W.
SGDiS000004757. SWP1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 194175LumenalSequence analysisAdd
BLAST
Transmembranei195 – 21521HelicalSequence analysisAdd
BLAST
Topological domaini216 – 22813CytoplasmicSequence analysisAdd
BLAST
Transmembranei229 – 24921HelicalSequence analysisAdd
BLAST
Topological domaini250 – 2523LumenalSequence analysis
Transmembranei253 – 27321HelicalSequence analysisAdd
BLAST
Topological domaini274 – 28613CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • oligosaccharyltransferase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 19191 PublicationAdd
BLAST
Chaini20 – 286267Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1PRO_0000021962Add
BLAST

Proteomic databases

MaxQBiQ02795.
TopDownProteomicsiQ02795.

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex, which appears to exist in two assemblies comprising OST1, OST2, OST4, OST5, STT3, SWP1, WPB1, and either OST3 or OST6. WBP1, SWP1 and OST2 probably form a subcomplex. May interact directly with OST1, OST2, OST4, STT3 and WBP1. Interacts with SEC61 and SSS1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SEC61P329153EBI-12666,EBI-16400
SSS1P351792EBI-12666,EBI-16406
WBP1P337674EBI-12666,EBI-12658

Protein-protein interaction databases

BioGridi35325. 221 interactions.
DIPiDIP-2460N.
IntActiQ02795. 37 interactions.
MINTiMINT-522209.

Structurei

3D structure databases

ProteinModelPortaliQ02795.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SWP1 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000115299.
InParanoidiQ02795.
KOiK12667.
OMAiANEDEGF.
OrthoDBiEOG092C4XN7.

Family and domain databases

InterProiIPR008814. Swp1.
[Graphical view]
PANTHERiPTHR12640:SF0. PTHR12640:SF0. 1 hit.
PfamiPF05817. Ribophorin_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02795-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQFFKTLAAL VSCISFVLAY VAQDVHVSFP STAGKSRVMI GKVEPRIGID
60 70 80 90 100
ETVPTTITVE DPNEVIQVNF AIESTNKPFQ NTLLIGLPNK NLEMAFEPEI
110 120 130 140 150
KDNGKLSMYK YRIDLAKLDA ALLQEASRSP EPIKATLILA SSTAKPKENL
160 170 180 190 200
FREILQLNLN FDVDHSDSSL VDKFGIKPEI HHIFHAEPKR VAKPIAVIFV
210 220 230 240 250
LIIFITILSL IVTWLNSCAA AFNNIPTGVT AVYFLGFIAT IVGFEVIFAR
260 270 280
YYLGTSIFET LFSSLYLGAP GLLTSTKFLR SFGQTI
Length:286
Mass (Da):31,653
Last modified:July 1, 1993 - v1
Checksum:i53D15B8857A534E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67705 Genomic DNA. Translation: CAA47943.1.
Z47071 Genomic DNA. Translation: CAA87364.1.
AY558418 Genomic DNA. Translation: AAS56744.1.
BK006946 Genomic DNA. Translation: DAA10045.1.
PIRiS28416.
RefSeqiNP_013869.1. NM_001182651.1.

Genome annotation databases

EnsemblFungiiYMR149W; YMR149W; YMR149W.
GeneIDi855180.
KEGGisce:YMR149W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67705 Genomic DNA. Translation: CAA47943.1.
Z47071 Genomic DNA. Translation: CAA87364.1.
AY558418 Genomic DNA. Translation: AAS56744.1.
BK006946 Genomic DNA. Translation: DAA10045.1.
PIRiS28416.
RefSeqiNP_013869.1. NM_001182651.1.

3D structure databases

ProteinModelPortaliQ02795.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35325. 221 interactions.
DIPiDIP-2460N.
IntActiQ02795. 37 interactions.
MINTiMINT-522209.

Proteomic databases

MaxQBiQ02795.
TopDownProteomicsiQ02795.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR149W; YMR149W; YMR149W.
GeneIDi855180.
KEGGisce:YMR149W.

Organism-specific databases

EuPathDBiFungiDB:YMR149W.
SGDiS000004757. SWP1.

Phylogenomic databases

HOGENOMiHOG000115299.
InParanoidiQ02795.
KOiK12667.
OMAiANEDEGF.
OrthoDBiEOG092C4XN7.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciYEAST:YMR149W-MONOMER.
BRENDAi2.4.99.18. 984.

Miscellaneous databases

PROiQ02795.

Family and domain databases

InterProiIPR008814. Swp1.
[Graphical view]
PANTHERiPTHR12640:SF0. PTHR12640:SF0. 1 hit.
PfamiPF05817. Ribophorin_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOSTD_YEAST
AccessioniPrimary (citable) accession number: Q02795
Secondary accession number(s): D6VZX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: September 7, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8450 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.