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Q02793 (SKI8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 134. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Antiviral protein SKI8
Alternative name(s):
Superkiller protein 8
Gene names
Name:SKI8
Synonyms:REC103
Ordered Locus Names:YGL213C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length397 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in double-strand break (DSB) formation during meiotic recombination through stabilization of SPO11 association with meiotic chromosome and helping SPO11 to recruit other DSB proteins like REC102 and REC104 to meiotic chromosomes. Also component of the SKI complex involved in 3'-mRNA degradation pathway. Represses dsRNA virus propagation by specifically blocking translation of viral mRNAs, perhaps recognizing the absence of CAP or poly(A). Essential for controlling the propagation of M double-stranded RNA (dsRNA) and thus for preventing virus-induced cytopathology. Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.16 Ref.17 Ref.18 Ref.20

Subunit structure

Component of the SKI complex composed of at least SKI2, SKI3 and SKI8. The SKI complex interacts with SKI7, which makes the link between the SKI complex and the exosome in order to perform mRNA degradation. Ref.11 Ref.12 Ref.18 Ref.20

Subcellular location

Cytoplasm. Nucleus. Chromosome. Note: Relocalizes from the cytoplasm to the nucleus and is recruited to chromosomes by SPO11 during meiotic prophase. Ref.14 Ref.18

Miscellaneous

Present with 1710 molecules/cell in log phase SD medium.

Sequence similarities

Contains 7 WD repeats.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 397397Antiviral protein SKI8
PRO_0000051218

Regions

Repeat21 – 4222WD 1
Repeat67 – 10135WD 2
Repeat126 – 15732WD 3
Repeat191 – 21323WD 4
Repeat238 – 26427WD 5
Repeat296 – 31924WD 6
Repeat359 – 39234WD 7

Secondary structure

.............................................................................. 397
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q02793 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: C5E7B9D2268F9727

FASTA39744,232
        10         20         30         40         50         60 
MSKVFIATAN AGKAHDADIF SVSACNSFTV SCSGDGYLKV WDNKLLDNEN PKDKSYSHFV 

        70         80         90        100        110        120 
HKSGLHHVDV LQAIERDAFE LCLVATTSFS GDLLFYRITR EDETKKVIFE KLDLLDSDMK 

       130        140        150        160        170        180 
KHSFWALKWG ASNDRLLSHR LVATDVKGTT YIWKFHPFAD ESNSLTLNWS PTLELQGTVE 

       190        200        210        220        230        240 
SPMTPSQFAT SVDISERGLI ATGFNNGTVQ ISELSTLRPL YNFESQHSMI NNSNSIRSVK 

       250        260        270        280        290        300 
FSPQGSLLAI AHDSNSFGCI TLYETEFGER IGSLSVPTHS SQASLGEFAH SSWVMSLSFN 

       310        320        330        340        350        360 
DSGETLCSAG WDGKLRFWDV KTKERITTLN MHCDDIEIEE DILAVDEHGD SLAEPGVFDV 

       370        380        390 
KFLKKGWRSG MGADLNESLC CVCLDRSIRW FREAGGK 

« Hide

References

« Hide 'large scale' references
[1]"A yeast antiviral protein, SKI8, shares a repeated amino acid sequence pattern with beta-subunits of G proteins and several other proteins."
Matsumoto Y., Sarkar G., Sommer S.S., Wickner R.B.
Yeast 9:43-51(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Analysis of 21.7 kb DNA sequence from the left arm of chromosome VII reveals 11 open reading frames: two correspond to new genes."
Feuermann M., Simeonava L., Souciet J.-L., Potier S.
Yeast 13:475-477(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-245.
Strain: ATCC 204508 / S288c.
[6]"Superkiller mutations in Saccharomyces cerevisiae suppress exclusion of M2 double-stranded RNA by L-A-HN and confer cold sensitivity in the presence of M and L-A-HN."
Ridley S.P., Sommer S.S., Wickner R.B.
Mol. Cell. Biol. 4:761-770(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Gene disruption indicates that the only essential function of the SKI8 chromosomal gene is to protect Saccharomyces cerevisiae from viral cytopathology."
Sommer S.S., Wickner R.B.
Virology 157:252-256(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Decoying the cap- mRNA degradation system by a double-stranded RNA virus and poly(A)- mRNA surveillance by a yeast antiviral system."
Masison D.C., Blanc A., Ribas J.C., Carroll K., Sonenberg N., Wickner R.B.
Mol. Cell. Biol. 15:2763-2771(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"Molecular and genetic analysis of REC103, an early meiotic recombination gene in yeast."
Gardiner J.M., Bullard S.A., Chrome C., Malone R.E.
Genetics 146:1265-1274(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"The 3' to 5' degradation of yeast mRNAs is a general mechanism for mRNA turnover that requires the SKI2 DEVH box protein and 3' to 5' exonucleases of the exosome complex."
Anderson J.S.J., Parker R.P.
EMBO J. 17:1497-1506(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"The yeast antiviral proteins Ski2p, Ski3p, and Ski8p exist as a complex in vivo."
Brown J.T., Bai X., Johnson A.W.
RNA 6:449-457(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN THE SKI COMPLEX.
[12]"Ski7p G protein interacts with the exosome and the Ski complex for 3'-to-5' mRNA decay in yeast."
Araki Y., Takahashi S., Kobayashi T., Kajiho H., Hoshino S., Katada T.
EMBO J. 20:4684-4693(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SKI7, FUNCTION OF THE SKI COMPLEX.
[13]"A cis-acting element known to block 3' mRNA degradation enhances expression of polyA-minus mRNA in wild-type yeast cells and phenocopies a ski mutant."
Brown J.T., Johnson A.W.
RNA 7:1566-1577(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE SKI COMPLEX.
[14]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[15]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[16]"Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virus."
Kushner D.B., Lindenbach B.D., Grdzelishvili V.Z., Noueiry A.O., Paul S.M., Ahlquist P.
Proc. Natl. Acad. Sci. U.S.A. 100:15764-15769(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[17]"Spatial organization and dynamics of the association of Rec102 and Rec104 with meiotic chromosomes."
Kee K., Protacio R.U., Arora C., Keeney S.
EMBO J. 23:1815-1824(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE SKI8-SPO11 COMPLEX.
[18]"Antiviral protein Ski8 is a direct partner of Spo11 in meiotic DNA break formation, independent of its cytoplasmic role in RNA metabolism."
Arora C., Kee K., Maleki S., Keeney S.
Mol. Cell 13:549-559(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SPO11.
[19]"Structure-based assembly of protein complexes in yeast."
Aloy P., Boettcher B., Ceulemans H., Leutwein C., Mellwig C., Fischer S., Gavin A.-C., Bork P., Superti-Furga G., Serrano L., Russell R.B.
Science 303:2026-2029(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: MODELING OF THE SKI COMPLEX 3D-STRUCTURE.
[20]"Both conserved and non-conserved regions of Spo11 are essential for meiotic recombination initiation in yeast."
Nag D.K., Pata J.D., Sironi M., Flood D.R., Hart A.M.
Mol. Genet. Genomics 276:313-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SPO11.
[21]"The structure of Ski8p, a protein regulating mRNA degradation: Implications for WD protein structure."
Madrona A.Y., Wilson D.K.
Protein Sci. 13:1557-1565(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
[22]"Crystal structure of Ski8p, a WD-repeat protein with dual roles in mRNA metabolism and meiotic recombination."
Cheng Z., Liu Y., Wang C., Parker R., Song H.
Protein Sci. 13:2673-2684(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M96058 mRNA. Translation: AAA35050.1.
Z72736 Genomic DNA. Translation: CAA96930.1.
BK006941 Genomic DNA. Translation: DAA07903.1.
PIRS30023.
RefSeqNP_011302.1. NM_001181078.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1S4UX-ray2.10X1-397[»]
1SQ9X-ray1.90A1-397[»]
4BUJX-ray3.70C/D/G/H1-397[»]
ProteinModelPortalQ02793.
SMRQ02793. Positions 2-397.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33043. 137 interactions.
DIPDIP-1653N.
IntActQ02793. 14 interactions.
MINTMINT-398286.
STRING4932.YGL213C.

Proteomic databases

PaxDbQ02793.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL213C; YGL213C; YGL213C.
GeneID852659.
KEGGsce:YGL213C.

Organism-specific databases

CYGDYGL213c.
SGDS000003181. SKI8.

Phylogenomic databases

eggNOGNOG283895.
HOGENOMHOG000247930.
KOK12601.
OMAGIRWYRE.
OrthoDBEOG7SXWCR.

Enzyme and pathway databases

BioCycYEAST:G3O-30690-MONOMER.

Gene expression databases

GenevestigatorQ02793.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF00400. WD40. 3 hits.
[Graphical view]
SMARTSM00320. WD40. 4 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 1 hit.
PROSITEPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ02793.
NextBio971939.

Entry information

Entry nameSKI8_YEAST
AccessionPrimary (citable) accession number: Q02793
Secondary accession number(s): D6VTU2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: April 16, 2014
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references