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Q02793

- SKI8_YEAST

UniProt

Q02793 - SKI8_YEAST

Protein

Antiviral protein SKI8

Gene

SKI8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    Involved in double-strand break (DSB) formation during meiotic recombination through stabilization of SPO11 association with meiotic chromosome and helping SPO11 to recruit other DSB proteins like REC102 and REC104 to meiotic chromosomes. Also component of the SKI complex involved in 3'-mRNA degradation pathway. Represses dsRNA virus propagation by specifically blocking translation of viral mRNAs, perhaps recognizing the absence of CAP or poly(A). Essential for controlling the propagation of M double-stranded RNA (dsRNA) and thus for preventing virus-induced cytopathology.12 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. defense response to virus Source: UniProtKB-KW
    2. nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay Source: SGD
    3. nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' Source: SGD
    4. nuclear-transcribed mRNA catabolic process, non-stop decay Source: SGD
    5. protein complex assembly Source: SGD
    6. protein-DNA complex assembly Source: SGD
    7. reciprocal meiotic recombination Source: SGD

    Keywords - Biological processi

    Antiviral defense, Meiosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30690-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Antiviral protein SKI8
    Alternative name(s):
    Superkiller protein 8
    Gene namesi
    Name:SKI8
    Synonyms:REC103
    Ordered Locus Names:YGL213C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL213c.
    SGDiS000003181. SKI8.

    Subcellular locationi

    Cytoplasm. Nucleus. Chromosome
    Note: Relocalizes from the cytoplasm to the nucleus and is recruited to chromosomes by SPO11 during meiotic prophase.

    GO - Cellular componenti

    1. nuclear chromosome Source: SGD
    2. Ski complex Source: SGD

    Keywords - Cellular componenti

    Chromosome, Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 397397Antiviral protein SKI8PRO_0000051218Add
    BLAST

    Proteomic databases

    MaxQBiQ02793.
    PaxDbiQ02793.

    Expressioni

    Gene expression databases

    GenevestigatoriQ02793.

    Interactioni

    Subunit structurei

    Component of the SKI complex composed of at least SKI2, SKI3 and SKI8. The SKI complex interacts with SKI7, which makes the link between the SKI complex and the exosome in order to perform mRNA degradation.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SKI2P3520710EBI-17260,EBI-1851
    SKI3P178837EBI-17260,EBI-1861
    SKI7Q084913EBI-17260,EBI-1389

    Protein-protein interaction databases

    BioGridi33043. 137 interactions.
    DIPiDIP-1653N.
    IntActiQ02793. 14 interactions.
    MINTiMINT-398286.
    STRINGi4932.YGL213C.

    Structurei

    Secondary structure

    1
    397
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 1310
    Beta strandi15 – 173
    Beta strandi19 – 246
    Beta strandi26 – 338
    Beta strandi36 – 449
    Helixi51 – 544
    Beta strandi55 – 595
    Beta strandi65 – 7511
    Turni76 – 783
    Beta strandi79 – 8810
    Beta strandi93 – 1008
    Turni102 – 1043
    Beta strandi107 – 1126
    Helixi119 – 1213
    Beta strandi124 – 1307
    Beta strandi139 – 1457
    Beta strandi150 – 16011
    Helixi161 – 1644
    Turni165 – 1673
    Beta strandi172 – 1798
    Beta strandi182 – 1854
    Beta strandi191 – 1944
    Beta strandi198 – 2036
    Beta strandi207 – 2137
    Turni214 – 2174
    Beta strandi218 – 2247
    Beta strandi236 – 2416
    Beta strandi247 – 2548
    Beta strandi257 – 2648
    Turni265 – 2673
    Beta strandi270 – 2745
    Beta strandi288 – 2925
    Beta strandi294 – 2996
    Beta strandi301 – 31010
    Beta strandi313 – 3197
    Turni320 – 3234
    Beta strandi324 – 3307
    Helixi333 – 3353
    Helixi339 – 3413
    Beta strandi357 – 3637
    Turni365 – 3673
    Beta strandi368 – 3703
    Beta strandi378 – 3836
    Turni384 – 3863
    Beta strandi387 – 3948

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1S4UX-ray2.10X1-397[»]
    1SQ9X-ray1.90A1-397[»]
    4BUJX-ray3.70C/D/G/H1-397[»]
    ProteinModelPortaliQ02793.
    SMRiQ02793. Positions 2-397.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ02793.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati21 – 4222WD 1Add
    BLAST
    Repeati67 – 10135WD 2Add
    BLAST
    Repeati126 – 15732WD 3Add
    BLAST
    Repeati191 – 21323WD 4Add
    BLAST
    Repeati238 – 26427WD 5Add
    BLAST
    Repeati296 – 31924WD 6Add
    BLAST
    Repeati359 – 39234WD 7Add
    BLAST

    Sequence similaritiesi

    Contains 7 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiNOG283895.
    HOGENOMiHOG000247930.
    KOiK12601.
    OMAiGIRWYRE.
    OrthoDBiEOG7SXWCR.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF00400. WD40. 3 hits.
    [Graphical view]
    SMARTiSM00320. WD40. 4 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS00678. WD_REPEATS_1. 2 hits.
    PS50082. WD_REPEATS_2. 1 hit.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q02793-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKVFIATAN AGKAHDADIF SVSACNSFTV SCSGDGYLKV WDNKLLDNEN    50
    PKDKSYSHFV HKSGLHHVDV LQAIERDAFE LCLVATTSFS GDLLFYRITR 100
    EDETKKVIFE KLDLLDSDMK KHSFWALKWG ASNDRLLSHR LVATDVKGTT 150
    YIWKFHPFAD ESNSLTLNWS PTLELQGTVE SPMTPSQFAT SVDISERGLI 200
    ATGFNNGTVQ ISELSTLRPL YNFESQHSMI NNSNSIRSVK FSPQGSLLAI 250
    AHDSNSFGCI TLYETEFGER IGSLSVPTHS SQASLGEFAH SSWVMSLSFN 300
    DSGETLCSAG WDGKLRFWDV KTKERITTLN MHCDDIEIEE DILAVDEHGD 350
    SLAEPGVFDV KFLKKGWRSG MGADLNESLC CVCLDRSIRW FREAGGK 397
    Length:397
    Mass (Da):44,232
    Last modified:July 1, 1993 - v1
    Checksum:iC5E7B9D2268F9727
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96058 mRNA. Translation: AAA35050.1.
    Z72736 Genomic DNA. Translation: CAA96930.1.
    BK006941 Genomic DNA. Translation: DAA07903.1.
    PIRiS30023.
    RefSeqiNP_011302.1. NM_001181078.1.

    Genome annotation databases

    EnsemblFungiiYGL213C; YGL213C; YGL213C.
    GeneIDi852659.
    KEGGisce:YGL213C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96058 mRNA. Translation: AAA35050.1 .
    Z72736 Genomic DNA. Translation: CAA96930.1 .
    BK006941 Genomic DNA. Translation: DAA07903.1 .
    PIRi S30023.
    RefSeqi NP_011302.1. NM_001181078.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1S4U X-ray 2.10 X 1-397 [» ]
    1SQ9 X-ray 1.90 A 1-397 [» ]
    4BUJ X-ray 3.70 C/D/G/H 1-397 [» ]
    ProteinModelPortali Q02793.
    SMRi Q02793. Positions 2-397.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33043. 137 interactions.
    DIPi DIP-1653N.
    IntActi Q02793. 14 interactions.
    MINTi MINT-398286.
    STRINGi 4932.YGL213C.

    Proteomic databases

    MaxQBi Q02793.
    PaxDbi Q02793.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL213C ; YGL213C ; YGL213C .
    GeneIDi 852659.
    KEGGi sce:YGL213C.

    Organism-specific databases

    CYGDi YGL213c.
    SGDi S000003181. SKI8.

    Phylogenomic databases

    eggNOGi NOG283895.
    HOGENOMi HOG000247930.
    KOi K12601.
    OMAi GIRWYRE.
    OrthoDBi EOG7SXWCR.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30690-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q02793.
    NextBioi 971939.

    Gene expression databases

    Genevestigatori Q02793.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF00400. WD40. 3 hits.
    [Graphical view ]
    SMARTi SM00320. WD40. 4 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    PROSITEi PS00678. WD_REPEATS_1. 2 hits.
    PS50082. WD_REPEATS_2. 1 hit.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A yeast antiviral protein, SKI8, shares a repeated amino acid sequence pattern with beta-subunits of G proteins and several other proteins."
      Matsumoto Y., Sarkar G., Sommer S.S., Wickner R.B.
      Yeast 9:43-51(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Analysis of 21.7 kb DNA sequence from the left arm of chromosome VII reveals 11 open reading frames: two correspond to new genes."
      Feuermann M., Simeonava L., Souciet J.-L., Potier S.
      Yeast 13:475-477(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
      Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
      Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-245.
      Strain: ATCC 204508 / S288c.
    6. "Superkiller mutations in Saccharomyces cerevisiae suppress exclusion of M2 double-stranded RNA by L-A-HN and confer cold sensitivity in the presence of M and L-A-HN."
      Ridley S.P., Sommer S.S., Wickner R.B.
      Mol. Cell. Biol. 4:761-770(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Gene disruption indicates that the only essential function of the SKI8 chromosomal gene is to protect Saccharomyces cerevisiae from viral cytopathology."
      Sommer S.S., Wickner R.B.
      Virology 157:252-256(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Decoying the cap- mRNA degradation system by a double-stranded RNA virus and poly(A)- mRNA surveillance by a yeast antiviral system."
      Masison D.C., Blanc A., Ribas J.C., Carroll K., Sonenberg N., Wickner R.B.
      Mol. Cell. Biol. 15:2763-2771(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Molecular and genetic analysis of REC103, an early meiotic recombination gene in yeast."
      Gardiner J.M., Bullard S.A., Chrome C., Malone R.E.
      Genetics 146:1265-1274(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "The 3' to 5' degradation of yeast mRNAs is a general mechanism for mRNA turnover that requires the SKI2 DEVH box protein and 3' to 5' exonucleases of the exosome complex."
      Anderson J.S.J., Parker R.P.
      EMBO J. 17:1497-1506(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "The yeast antiviral proteins Ski2p, Ski3p, and Ski8p exist as a complex in vivo."
      Brown J.T., Bai X., Johnson A.W.
      RNA 6:449-457(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE SKI COMPLEX.
    12. "Ski7p G protein interacts with the exosome and the Ski complex for 3'-to-5' mRNA decay in yeast."
      Araki Y., Takahashi S., Kobayashi T., Kajiho H., Hoshino S., Katada T.
      EMBO J. 20:4684-4693(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SKI7, FUNCTION OF THE SKI COMPLEX.
    13. "A cis-acting element known to block 3' mRNA degradation enhances expression of polyA-minus mRNA in wild-type yeast cells and phenocopies a ski mutant."
      Brown J.T., Johnson A.W.
      RNA 7:1566-1577(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE SKI COMPLEX.
    14. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    15. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    16. "Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virus."
      Kushner D.B., Lindenbach B.D., Grdzelishvili V.Z., Noueiry A.O., Paul S.M., Ahlquist P.
      Proc. Natl. Acad. Sci. U.S.A. 100:15764-15769(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    17. "Spatial organization and dynamics of the association of Rec102 and Rec104 with meiotic chromosomes."
      Kee K., Protacio R.U., Arora C., Keeney S.
      EMBO J. 23:1815-1824(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE SKI8-SPO11 COMPLEX.
    18. "Antiviral protein Ski8 is a direct partner of Spo11 in meiotic DNA break formation, independent of its cytoplasmic role in RNA metabolism."
      Arora C., Kee K., Maleki S., Keeney S.
      Mol. Cell 13:549-559(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SPO11.
    19. Cited for: MODELING OF THE SKI COMPLEX 3D-STRUCTURE.
    20. "Both conserved and non-conserved regions of Spo11 are essential for meiotic recombination initiation in yeast."
      Nag D.K., Pata J.D., Sironi M., Flood D.R., Hart A.M.
      Mol. Genet. Genomics 276:313-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SPO11.
    21. "The structure of Ski8p, a protein regulating mRNA degradation: Implications for WD protein structure."
      Madrona A.Y., Wilson D.K.
      Protein Sci. 13:1557-1565(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
    22. "Crystal structure of Ski8p, a WD-repeat protein with dual roles in mRNA metabolism and meiotic recombination."
      Cheng Z., Liu Y., Wang C., Parker R., Song H.
      Protein Sci. 13:2673-2684(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).

    Entry informationi

    Entry nameiSKI8_YEAST
    AccessioniPrimary (citable) accession number: Q02793
    Secondary accession number(s): D6VTU2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 137 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1710 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3