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Q02793

- SKI8_YEAST

UniProt

Q02793 - SKI8_YEAST

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Protein

Antiviral protein SKI8

Gene
SKI8, REC103, YGL213C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in double-strand break (DSB) formation during meiotic recombination through stabilization of SPO11 association with meiotic chromosome and helping SPO11 to recruit other DSB proteins like REC102 and REC104 to meiotic chromosomes. Also component of the SKI complex involved in 3'-mRNA degradation pathway. Represses dsRNA virus propagation by specifically blocking translation of viral mRNAs, perhaps recognizing the absence of CAP or poly(A). Essential for controlling the propagation of M double-stranded RNA (dsRNA) and thus for preventing virus-induced cytopathology.12 Publications

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. defense response to virus Source: UniProtKB-KW
  2. nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay Source: SGD
  3. nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' Source: SGD
  4. nuclear-transcribed mRNA catabolic process, non-stop decay Source: SGD
  5. protein complex assembly Source: SGD
  6. protein-DNA complex assembly Source: SGD
  7. reciprocal meiotic recombination Source: SGD
Complete GO annotation...

Keywords - Biological processi

Antiviral defense, Meiosis

Enzyme and pathway databases

BioCyciYEAST:G3O-30690-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Antiviral protein SKI8
Alternative name(s):
Superkiller protein 8
Gene namesi
Name:SKI8
Synonyms:REC103
Ordered Locus Names:YGL213C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL213c.
SGDiS000003181. SKI8.

Subcellular locationi

Cytoplasm. Nucleus. Chromosome
Note: Relocalizes from the cytoplasm to the nucleus and is recruited to chromosomes by SPO11 during meiotic prophase.2 Publications

GO - Cellular componenti

  1. nuclear chromosome Source: SGD
  2. Ski complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 397397Antiviral protein SKI8PRO_0000051218Add
BLAST

Proteomic databases

MaxQBiQ02793.
PaxDbiQ02793.

Expressioni

Gene expression databases

GenevestigatoriQ02793.

Interactioni

Subunit structurei

Component of the SKI complex composed of at least SKI2, SKI3 and SKI8. The SKI complex interacts with SKI7, which makes the link between the SKI complex and the exosome in order to perform mRNA degradation.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SKI2P3520710EBI-17260,EBI-1851
SKI3P178837EBI-17260,EBI-1861
SKI7Q084913EBI-17260,EBI-1389

Protein-protein interaction databases

BioGridi33043. 137 interactions.
DIPiDIP-1653N.
IntActiQ02793. 14 interactions.
MINTiMINT-398286.
STRINGi4932.YGL213C.

Structurei

Secondary structure

1
397
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 1310
Beta strandi15 – 173
Beta strandi19 – 246
Beta strandi26 – 338
Beta strandi36 – 449
Helixi51 – 544
Beta strandi55 – 595
Beta strandi65 – 7511
Turni76 – 783
Beta strandi79 – 8810
Beta strandi93 – 1008
Turni102 – 1043
Beta strandi107 – 1126
Helixi119 – 1213
Beta strandi124 – 1307
Beta strandi139 – 1457
Beta strandi150 – 16011
Helixi161 – 1644
Turni165 – 1673
Beta strandi172 – 1798
Beta strandi182 – 1854
Beta strandi191 – 1944
Beta strandi198 – 2036
Beta strandi207 – 2137
Turni214 – 2174
Beta strandi218 – 2247
Beta strandi236 – 2416
Beta strandi247 – 2548
Beta strandi257 – 2648
Turni265 – 2673
Beta strandi270 – 2745
Beta strandi288 – 2925
Beta strandi294 – 2996
Beta strandi301 – 31010
Beta strandi313 – 3197
Turni320 – 3234
Beta strandi324 – 3307
Helixi333 – 3353
Helixi339 – 3413
Beta strandi357 – 3637
Turni365 – 3673
Beta strandi368 – 3703
Beta strandi378 – 3836
Turni384 – 3863
Beta strandi387 – 3948

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1S4UX-ray2.10X1-397[»]
1SQ9X-ray1.90A1-397[»]
4BUJX-ray3.70C/D/G/H1-397[»]
ProteinModelPortaliQ02793.
SMRiQ02793. Positions 2-397.

Miscellaneous databases

EvolutionaryTraceiQ02793.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati21 – 4222WD 1Add
BLAST
Repeati67 – 10135WD 2Add
BLAST
Repeati126 – 15732WD 3Add
BLAST
Repeati191 – 21323WD 4Add
BLAST
Repeati238 – 26427WD 5Add
BLAST
Repeati296 – 31924WD 6Add
BLAST
Repeati359 – 39234WD 7Add
BLAST

Sequence similaritiesi

Contains 7 WD repeats.

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiNOG283895.
HOGENOMiHOG000247930.
KOiK12601.
OMAiGIRWYRE.
OrthoDBiEOG7SXWCR.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 3 hits.
[Graphical view]
SMARTiSM00320. WD40. 4 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q02793-1 [UniParc]FASTAAdd to Basket

« Hide

MSKVFIATAN AGKAHDADIF SVSACNSFTV SCSGDGYLKV WDNKLLDNEN    50
PKDKSYSHFV HKSGLHHVDV LQAIERDAFE LCLVATTSFS GDLLFYRITR 100
EDETKKVIFE KLDLLDSDMK KHSFWALKWG ASNDRLLSHR LVATDVKGTT 150
YIWKFHPFAD ESNSLTLNWS PTLELQGTVE SPMTPSQFAT SVDISERGLI 200
ATGFNNGTVQ ISELSTLRPL YNFESQHSMI NNSNSIRSVK FSPQGSLLAI 250
AHDSNSFGCI TLYETEFGER IGSLSVPTHS SQASLGEFAH SSWVMSLSFN 300
DSGETLCSAG WDGKLRFWDV KTKERITTLN MHCDDIEIEE DILAVDEHGD 350
SLAEPGVFDV KFLKKGWRSG MGADLNESLC CVCLDRSIRW FREAGGK 397
Length:397
Mass (Da):44,232
Last modified:July 1, 1993 - v1
Checksum:iC5E7B9D2268F9727
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M96058 mRNA. Translation: AAA35050.1.
Z72736 Genomic DNA. Translation: CAA96930.1.
BK006941 Genomic DNA. Translation: DAA07903.1.
PIRiS30023.
RefSeqiNP_011302.1. NM_001181078.1.

Genome annotation databases

EnsemblFungiiYGL213C; YGL213C; YGL213C.
GeneIDi852659.
KEGGisce:YGL213C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M96058 mRNA. Translation: AAA35050.1 .
Z72736 Genomic DNA. Translation: CAA96930.1 .
BK006941 Genomic DNA. Translation: DAA07903.1 .
PIRi S30023.
RefSeqi NP_011302.1. NM_001181078.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1S4U X-ray 2.10 X 1-397 [» ]
1SQ9 X-ray 1.90 A 1-397 [» ]
4BUJ X-ray 3.70 C/D/G/H 1-397 [» ]
ProteinModelPortali Q02793.
SMRi Q02793. Positions 2-397.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33043. 137 interactions.
DIPi DIP-1653N.
IntActi Q02793. 14 interactions.
MINTi MINT-398286.
STRINGi 4932.YGL213C.

Proteomic databases

MaxQBi Q02793.
PaxDbi Q02793.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL213C ; YGL213C ; YGL213C .
GeneIDi 852659.
KEGGi sce:YGL213C.

Organism-specific databases

CYGDi YGL213c.
SGDi S000003181. SKI8.

Phylogenomic databases

eggNOGi NOG283895.
HOGENOMi HOG000247930.
KOi K12601.
OMAi GIRWYRE.
OrthoDBi EOG7SXWCR.

Enzyme and pathway databases

BioCyci YEAST:G3O-30690-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q02793.
NextBioi 971939.

Gene expression databases

Genevestigatori Q02793.

Family and domain databases

Gene3Di 2.130.10.10. 1 hit.
InterProi IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF00400. WD40. 3 hits.
[Graphical view ]
SMARTi SM00320. WD40. 4 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 1 hit.
PROSITEi PS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A yeast antiviral protein, SKI8, shares a repeated amino acid sequence pattern with beta-subunits of G proteins and several other proteins."
    Matsumoto Y., Sarkar G., Sommer S.S., Wickner R.B.
    Yeast 9:43-51(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Analysis of 21.7 kb DNA sequence from the left arm of chromosome VII reveals 11 open reading frames: two correspond to new genes."
    Feuermann M., Simeonava L., Souciet J.-L., Potier S.
    Yeast 13:475-477(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
    Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
    Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-245.
    Strain: ATCC 204508 / S288c.
  6. "Superkiller mutations in Saccharomyces cerevisiae suppress exclusion of M2 double-stranded RNA by L-A-HN and confer cold sensitivity in the presence of M and L-A-HN."
    Ridley S.P., Sommer S.S., Wickner R.B.
    Mol. Cell. Biol. 4:761-770(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Gene disruption indicates that the only essential function of the SKI8 chromosomal gene is to protect Saccharomyces cerevisiae from viral cytopathology."
    Sommer S.S., Wickner R.B.
    Virology 157:252-256(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Decoying the cap- mRNA degradation system by a double-stranded RNA virus and poly(A)- mRNA surveillance by a yeast antiviral system."
    Masison D.C., Blanc A., Ribas J.C., Carroll K., Sonenberg N., Wickner R.B.
    Mol. Cell. Biol. 15:2763-2771(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Molecular and genetic analysis of REC103, an early meiotic recombination gene in yeast."
    Gardiner J.M., Bullard S.A., Chrome C., Malone R.E.
    Genetics 146:1265-1274(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "The 3' to 5' degradation of yeast mRNAs is a general mechanism for mRNA turnover that requires the SKI2 DEVH box protein and 3' to 5' exonucleases of the exosome complex."
    Anderson J.S.J., Parker R.P.
    EMBO J. 17:1497-1506(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "The yeast antiviral proteins Ski2p, Ski3p, and Ski8p exist as a complex in vivo."
    Brown J.T., Bai X., Johnson A.W.
    RNA 6:449-457(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE SKI COMPLEX.
  12. "Ski7p G protein interacts with the exosome and the Ski complex for 3'-to-5' mRNA decay in yeast."
    Araki Y., Takahashi S., Kobayashi T., Kajiho H., Hoshino S., Katada T.
    EMBO J. 20:4684-4693(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SKI7, FUNCTION OF THE SKI COMPLEX.
  13. "A cis-acting element known to block 3' mRNA degradation enhances expression of polyA-minus mRNA in wild-type yeast cells and phenocopies a ski mutant."
    Brown J.T., Johnson A.W.
    RNA 7:1566-1577(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE SKI COMPLEX.
  14. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  15. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  16. "Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virus."
    Kushner D.B., Lindenbach B.D., Grdzelishvili V.Z., Noueiry A.O., Paul S.M., Ahlquist P.
    Proc. Natl. Acad. Sci. U.S.A. 100:15764-15769(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  17. "Spatial organization and dynamics of the association of Rec102 and Rec104 with meiotic chromosomes."
    Kee K., Protacio R.U., Arora C., Keeney S.
    EMBO J. 23:1815-1824(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE SKI8-SPO11 COMPLEX.
  18. "Antiviral protein Ski8 is a direct partner of Spo11 in meiotic DNA break formation, independent of its cytoplasmic role in RNA metabolism."
    Arora C., Kee K., Maleki S., Keeney S.
    Mol. Cell 13:549-559(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SPO11.
  19. Cited for: MODELING OF THE SKI COMPLEX 3D-STRUCTURE.
  20. "Both conserved and non-conserved regions of Spo11 are essential for meiotic recombination initiation in yeast."
    Nag D.K., Pata J.D., Sironi M., Flood D.R., Hart A.M.
    Mol. Genet. Genomics 276:313-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SPO11.
  21. "The structure of Ski8p, a protein regulating mRNA degradation: Implications for WD protein structure."
    Madrona A.Y., Wilson D.K.
    Protein Sci. 13:1557-1565(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
  22. "Crystal structure of Ski8p, a WD-repeat protein with dual roles in mRNA metabolism and meiotic recombination."
    Cheng Z., Liu Y., Wang C., Parker R., Song H.
    Protein Sci. 13:2673-2684(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).

Entry informationi

Entry nameiSKI8_YEAST
AccessioniPrimary (citable) accession number: Q02793
Secondary accession number(s): D6VTU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: June 11, 2014
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1710 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

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