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Protein

Antiviral protein SKI8

Gene

SKI8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in double-strand break (DSB) formation during meiotic recombination through stabilization of SPO11 association with meiotic chromosome and helping SPO11 to recruit other DSB proteins like REC102 and REC104 to meiotic chromosomes. Also component of the SKI complex involved in 3'-mRNA degradation pathway. Represses dsRNA virus propagation by specifically blocking translation of viral mRNAs, perhaps recognizing the absence of CAP or poly(A). Essential for controlling the propagation of M double-stranded RNA (dsRNA) and thus for preventing virus-induced cytopathology.12 Publications

GO - Biological processi

  • defense response to virus Source: UniProtKB-KW
  • nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay Source: SGD
  • nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' Source: SGD
  • nuclear-transcribed mRNA catabolic process, non-stop decay Source: SGD
  • protein complex assembly Source: SGD
  • protein-DNA complex assembly Source: SGD
  • reciprocal meiotic recombination Source: SGD
Complete GO annotation...

Keywords - Biological processi

Antiviral defense, Meiosis

Enzyme and pathway databases

BioCyciYEAST:G3O-30690-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Antiviral protein SKI8
Alternative name(s):
Superkiller protein 8
Gene namesi
Name:SKI8
Synonyms:REC103
Ordered Locus Names:YGL213C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL213C.
SGDiS000003181. SKI8.

Subcellular locationi

  • Cytoplasm
  • Nucleus
  • Chromosome

  • Note: Relocalizes from the cytoplasm to the nucleus and is recruited to chromosomes by SPO11 during meiotic prophase.

GO - Cellular componenti

  • nuclear chromosome Source: SGD
  • Ski complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000512181 – 397Antiviral protein SKI8Add BLAST397

Proteomic databases

MaxQBiQ02793.
PRIDEiQ02793.

Interactioni

Subunit structurei

Component of the SKI complex composed of at least SKI2, SKI3 and SKI8. The SKI complex interacts with SKI7, which makes the link between the SKI complex and the exosome in order to perform mRNA degradation.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SKI2P3520710EBI-17260,EBI-1851
SKI3P178837EBI-17260,EBI-1861
SKI7Q084913EBI-17260,EBI-1389

Protein-protein interaction databases

BioGridi33043. 139 interactors.
DIPiDIP-1653N.
IntActiQ02793. 15 interactors.
MINTiMINT-398286.

Structurei

Secondary structure

1397
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 13Combined sources10
Beta strandi15 – 17Combined sources3
Beta strandi19 – 24Combined sources6
Beta strandi26 – 33Combined sources8
Beta strandi36 – 44Combined sources9
Helixi51 – 54Combined sources4
Beta strandi55 – 59Combined sources5
Beta strandi65 – 75Combined sources11
Turni76 – 78Combined sources3
Beta strandi79 – 88Combined sources10
Beta strandi93 – 100Combined sources8
Turni102 – 104Combined sources3
Beta strandi107 – 112Combined sources6
Helixi119 – 121Combined sources3
Beta strandi124 – 130Combined sources7
Beta strandi139 – 145Combined sources7
Beta strandi150 – 160Combined sources11
Helixi161 – 164Combined sources4
Turni165 – 167Combined sources3
Beta strandi172 – 179Combined sources8
Beta strandi182 – 185Combined sources4
Beta strandi191 – 194Combined sources4
Beta strandi198 – 203Combined sources6
Beta strandi207 – 213Combined sources7
Turni214 – 217Combined sources4
Beta strandi218 – 224Combined sources7
Beta strandi236 – 241Combined sources6
Beta strandi247 – 254Combined sources8
Beta strandi257 – 264Combined sources8
Turni265 – 267Combined sources3
Beta strandi270 – 274Combined sources5
Beta strandi288 – 292Combined sources5
Beta strandi294 – 299Combined sources6
Beta strandi301 – 310Combined sources10
Beta strandi313 – 319Combined sources7
Turni320 – 323Combined sources4
Beta strandi324 – 330Combined sources7
Helixi333 – 335Combined sources3
Helixi339 – 341Combined sources3
Beta strandi357 – 363Combined sources7
Turni365 – 367Combined sources3
Beta strandi368 – 370Combined sources3
Beta strandi378 – 383Combined sources6
Turni384 – 386Combined sources3
Beta strandi387 – 394Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S4UX-ray2.10X1-397[»]
1SQ9X-ray1.90A1-397[»]
4BUJX-ray3.70C/D/G/H1-397[»]
ProteinModelPortaliQ02793.
SMRiQ02793.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ02793.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati21 – 42WD 1Add BLAST22
Repeati67 – 101WD 2Add BLAST35
Repeati126 – 157WD 3Add BLAST32
Repeati191 – 213WD 4Add BLAST23
Repeati238 – 264WD 5Add BLAST27
Repeati296 – 319WD 6Add BLAST24
Repeati359 – 392WD 7Add BLAST34

Sequence similaritiesi

Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

HOGENOMiHOG000247930.
InParanoidiQ02793.
KOiK12601.
OMAiCVCLDRS.
OrthoDBiEOG092C3CFK.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM00320. WD40. 4 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q02793-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKVFIATAN AGKAHDADIF SVSACNSFTV SCSGDGYLKV WDNKLLDNEN
60 70 80 90 100
PKDKSYSHFV HKSGLHHVDV LQAIERDAFE LCLVATTSFS GDLLFYRITR
110 120 130 140 150
EDETKKVIFE KLDLLDSDMK KHSFWALKWG ASNDRLLSHR LVATDVKGTT
160 170 180 190 200
YIWKFHPFAD ESNSLTLNWS PTLELQGTVE SPMTPSQFAT SVDISERGLI
210 220 230 240 250
ATGFNNGTVQ ISELSTLRPL YNFESQHSMI NNSNSIRSVK FSPQGSLLAI
260 270 280 290 300
AHDSNSFGCI TLYETEFGER IGSLSVPTHS SQASLGEFAH SSWVMSLSFN
310 320 330 340 350
DSGETLCSAG WDGKLRFWDV KTKERITTLN MHCDDIEIEE DILAVDEHGD
360 370 380 390
SLAEPGVFDV KFLKKGWRSG MGADLNESLC CVCLDRSIRW FREAGGK
Length:397
Mass (Da):44,232
Last modified:July 1, 1993 - v1
Checksum:iC5E7B9D2268F9727
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96058 mRNA. Translation: AAA35050.1.
Z72736 Genomic DNA. Translation: CAA96930.1.
BK006941 Genomic DNA. Translation: DAA07903.1.
PIRiS30023.
RefSeqiNP_011302.1. NM_001181078.1.

Genome annotation databases

EnsemblFungiiYGL213C; YGL213C; YGL213C.
GeneIDi852659.
KEGGisce:YGL213C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96058 mRNA. Translation: AAA35050.1.
Z72736 Genomic DNA. Translation: CAA96930.1.
BK006941 Genomic DNA. Translation: DAA07903.1.
PIRiS30023.
RefSeqiNP_011302.1. NM_001181078.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S4UX-ray2.10X1-397[»]
1SQ9X-ray1.90A1-397[»]
4BUJX-ray3.70C/D/G/H1-397[»]
ProteinModelPortaliQ02793.
SMRiQ02793.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33043. 139 interactors.
DIPiDIP-1653N.
IntActiQ02793. 15 interactors.
MINTiMINT-398286.

Proteomic databases

MaxQBiQ02793.
PRIDEiQ02793.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL213C; YGL213C; YGL213C.
GeneIDi852659.
KEGGisce:YGL213C.

Organism-specific databases

EuPathDBiFungiDB:YGL213C.
SGDiS000003181. SKI8.

Phylogenomic databases

HOGENOMiHOG000247930.
InParanoidiQ02793.
KOiK12601.
OMAiCVCLDRS.
OrthoDBiEOG092C3CFK.

Enzyme and pathway databases

BioCyciYEAST:G3O-30690-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ02793.
PROiQ02793.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM00320. WD40. 4 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSKI8_YEAST
AccessioniPrimary (citable) accession number: Q02793
Secondary accession number(s): D6VTU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 2, 2016
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1710 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.