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Protein

Antiviral protein SKI8

Gene

SKI8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in double-strand break (DSB) formation during meiotic recombination through stabilization of SPO11 association with meiotic chromosome and helping SPO11 to recruit other DSB proteins like REC102 and REC104 to meiotic chromosomes. Also component of the SKI complex involved in 3'-mRNA degradation pathway. Represses dsRNA virus propagation by specifically blocking translation of viral mRNAs, perhaps recognizing the absence of CAP or poly(A). Essential for controlling the propagation of M double-stranded RNA (dsRNA) and thus for preventing virus-induced cytopathology.12 Publications

GO - Biological processi

  • defense response to virus Source: UniProtKB-KW
  • nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay Source: SGD
  • nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' Source: SGD
  • nuclear-transcribed mRNA catabolic process, non-stop decay Source: SGD
  • protein complex assembly Source: SGD
  • protein-DNA complex assembly Source: SGD
  • reciprocal meiotic recombination Source: SGD
Complete GO annotation...

Keywords - Biological processi

Antiviral defense, Meiosis

Enzyme and pathway databases

BioCyciYEAST:G3O-30690-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Antiviral protein SKI8
Alternative name(s):
Superkiller protein 8
Gene namesi
Name:SKI8
Synonyms:REC103
Ordered Locus Names:YGL213C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL213C.
SGDiS000003181. SKI8.

Subcellular locationi

  • Cytoplasm
  • Nucleus
  • Chromosome

  • Note: Relocalizes from the cytoplasm to the nucleus and is recruited to chromosomes by SPO11 during meiotic prophase.

GO - Cellular componenti

  • nuclear chromosome Source: SGD
  • Ski complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 397397Antiviral protein SKI8PRO_0000051218Add
BLAST

Proteomic databases

MaxQBiQ02793.

Interactioni

Subunit structurei

Component of the SKI complex composed of at least SKI2, SKI3 and SKI8. The SKI complex interacts with SKI7, which makes the link between the SKI complex and the exosome in order to perform mRNA degradation.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SKI2P3520710EBI-17260,EBI-1851
SKI3P178837EBI-17260,EBI-1861
SKI7Q084913EBI-17260,EBI-1389

Protein-protein interaction databases

BioGridi33043. 138 interactions.
DIPiDIP-1653N.
IntActiQ02793. 15 interactions.
MINTiMINT-398286.

Structurei

Secondary structure

1
397
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 1310Combined sources
Beta strandi15 – 173Combined sources
Beta strandi19 – 246Combined sources
Beta strandi26 – 338Combined sources
Beta strandi36 – 449Combined sources
Helixi51 – 544Combined sources
Beta strandi55 – 595Combined sources
Beta strandi65 – 7511Combined sources
Turni76 – 783Combined sources
Beta strandi79 – 8810Combined sources
Beta strandi93 – 1008Combined sources
Turni102 – 1043Combined sources
Beta strandi107 – 1126Combined sources
Helixi119 – 1213Combined sources
Beta strandi124 – 1307Combined sources
Beta strandi139 – 1457Combined sources
Beta strandi150 – 16011Combined sources
Helixi161 – 1644Combined sources
Turni165 – 1673Combined sources
Beta strandi172 – 1798Combined sources
Beta strandi182 – 1854Combined sources
Beta strandi191 – 1944Combined sources
Beta strandi198 – 2036Combined sources
Beta strandi207 – 2137Combined sources
Turni214 – 2174Combined sources
Beta strandi218 – 2247Combined sources
Beta strandi236 – 2416Combined sources
Beta strandi247 – 2548Combined sources
Beta strandi257 – 2648Combined sources
Turni265 – 2673Combined sources
Beta strandi270 – 2745Combined sources
Beta strandi288 – 2925Combined sources
Beta strandi294 – 2996Combined sources
Beta strandi301 – 31010Combined sources
Beta strandi313 – 3197Combined sources
Turni320 – 3234Combined sources
Beta strandi324 – 3307Combined sources
Helixi333 – 3353Combined sources
Helixi339 – 3413Combined sources
Beta strandi357 – 3637Combined sources
Turni365 – 3673Combined sources
Beta strandi368 – 3703Combined sources
Beta strandi378 – 3836Combined sources
Turni384 – 3863Combined sources
Beta strandi387 – 3948Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1S4UX-ray2.10X1-397[»]
1SQ9X-ray1.90A1-397[»]
4BUJX-ray3.70C/D/G/H1-397[»]
ProteinModelPortaliQ02793.
SMRiQ02793. Positions 2-397.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ02793.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati21 – 4222WD 1Add
BLAST
Repeati67 – 10135WD 2Add
BLAST
Repeati126 – 15732WD 3Add
BLAST
Repeati191 – 21323WD 4Add
BLAST
Repeati238 – 26427WD 5Add
BLAST
Repeati296 – 31924WD 6Add
BLAST
Repeati359 – 39234WD 7Add
BLAST

Sequence similaritiesi

Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

HOGENOMiHOG000247930.
InParanoidiQ02793.
KOiK12601.
OMAiCVCLDRS.
OrthoDBiEOG092C3CFK.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM00320. WD40. 4 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q02793-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKVFIATAN AGKAHDADIF SVSACNSFTV SCSGDGYLKV WDNKLLDNEN
60 70 80 90 100
PKDKSYSHFV HKSGLHHVDV LQAIERDAFE LCLVATTSFS GDLLFYRITR
110 120 130 140 150
EDETKKVIFE KLDLLDSDMK KHSFWALKWG ASNDRLLSHR LVATDVKGTT
160 170 180 190 200
YIWKFHPFAD ESNSLTLNWS PTLELQGTVE SPMTPSQFAT SVDISERGLI
210 220 230 240 250
ATGFNNGTVQ ISELSTLRPL YNFESQHSMI NNSNSIRSVK FSPQGSLLAI
260 270 280 290 300
AHDSNSFGCI TLYETEFGER IGSLSVPTHS SQASLGEFAH SSWVMSLSFN
310 320 330 340 350
DSGETLCSAG WDGKLRFWDV KTKERITTLN MHCDDIEIEE DILAVDEHGD
360 370 380 390
SLAEPGVFDV KFLKKGWRSG MGADLNESLC CVCLDRSIRW FREAGGK
Length:397
Mass (Da):44,232
Last modified:July 1, 1993 - v1
Checksum:iC5E7B9D2268F9727
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96058 mRNA. Translation: AAA35050.1.
Z72736 Genomic DNA. Translation: CAA96930.1.
BK006941 Genomic DNA. Translation: DAA07903.1.
PIRiS30023.
RefSeqiNP_011302.1. NM_001181078.1.

Genome annotation databases

EnsemblFungiiYGL213C; YGL213C; YGL213C.
GeneIDi852659.
KEGGisce:YGL213C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96058 mRNA. Translation: AAA35050.1.
Z72736 Genomic DNA. Translation: CAA96930.1.
BK006941 Genomic DNA. Translation: DAA07903.1.
PIRiS30023.
RefSeqiNP_011302.1. NM_001181078.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1S4UX-ray2.10X1-397[»]
1SQ9X-ray1.90A1-397[»]
4BUJX-ray3.70C/D/G/H1-397[»]
ProteinModelPortaliQ02793.
SMRiQ02793. Positions 2-397.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33043. 138 interactions.
DIPiDIP-1653N.
IntActiQ02793. 15 interactions.
MINTiMINT-398286.

Proteomic databases

MaxQBiQ02793.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL213C; YGL213C; YGL213C.
GeneIDi852659.
KEGGisce:YGL213C.

Organism-specific databases

EuPathDBiFungiDB:YGL213C.
SGDiS000003181. SKI8.

Phylogenomic databases

HOGENOMiHOG000247930.
InParanoidiQ02793.
KOiK12601.
OMAiCVCLDRS.
OrthoDBiEOG092C3CFK.

Enzyme and pathway databases

BioCyciYEAST:G3O-30690-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ02793.
PROiQ02793.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM00320. WD40. 4 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSKI8_YEAST
AccessioniPrimary (citable) accession number: Q02793
Secondary accession number(s): D6VTU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: September 7, 2016
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1710 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.