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Protein

Monothiol glutaredoxin-5, mitochondrial

Gene

GRX5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Monothiol glutaredoxin involved in iron-sulfur biogenesis. Required for normal iron homeostasis. Protects cells against oxidative damage due to reactive oxygen species.1 Publication

Redox potential

E0 is -175 mV.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei52 – 521GlutathioneBy similarity
Metal bindingi60 – 601Iron-sulfur (2Fe-2S); shared with dimeric partnerBy similarity
Binding sitei104 – 1041Glutathione; via amide nitrogen and carbonyl oxygenBy similarity

GO - Molecular functioni

GO - Biological processi

  • cell redox homeostasis Source: InterPro
  • cellular response to oxidative stress Source: SGD
  • iron-sulfur cluster assembly Source: SGD
  • response to osmotic stress Source: SGD
Complete GO annotation...

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33970-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Monothiol glutaredoxin-5, mitochondrial
Gene namesi
Name:GRX5
Ordered Locus Names:YPL059W
ORF Names:LPE13W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL059W.
SGDiS000005980. GRX5.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2929Mitochondrion1 PublicationAdd
BLAST
Chaini30 – 150121Monothiol glutaredoxin-5, mitochondrialPRO_0000011632Add
BLAST

Proteomic databases

MaxQBiQ02784.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi36121. 30 interactions.
DIPiDIP-1352N.
IntActiQ02784. 4 interactions.
MINTiMINT-392130.

Structurei

Secondary structure

1
150
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi32 – 4312Combined sources
Beta strandi46 – 538Combined sources
Beta strandi55 – 584Combined sources
Helixi62 – 7312Combined sources
Helixi77 – 793Combined sources
Beta strandi80 – 845Combined sources
Helixi89 – 9911Combined sources
Beta strandi106 – 1094Combined sources
Beta strandi112 – 1165Combined sources
Helixi117 – 12610Combined sources
Helixi128 – 1358Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3GX8X-ray1.67A30-150[»]
ProteinModelPortaliQ02784.
SMRiQ02784. Positions 31-141.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ02784.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 140106GlutaredoxinPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni92 – 965Glutathione bindingBy similarity
Regioni117 – 1182Glutathione bindingBy similarity

Sequence similaritiesi

Contains 1 glutaredoxin domain.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Transit peptide

Phylogenomic databases

GeneTreeiENSGT00550000075082.
HOGENOMiHOG000095211.
InParanoidiQ02784.
KOiK07390.
OMAiFNCLEDQ.
OrthoDBiEOG092C3TIP.

Family and domain databases

CDDicd03028. GRX_PICOT_like. 1 hit.
Gene3Di3.40.30.10. 1 hit.
InterProiIPR002109. Glutaredoxin.
IPR033658. GRX_PICOT_like.
IPR004480. Monothiol_GRX-rel.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR10293. PTHR10293. 1 hit.
PfamiPF00462. Glutaredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR00365. TIGR00365. 1 hit.
PROSITEiPS51354. GLUTAREDOXIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02784-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLPKFNPIR SFSPILRAKT LLRYQNRMYL STEIRKAIED AIESAPVVLF
60 70 80 90 100
MKGTPEFPKC GFSRATIGLL GNQGVDPAKF AAYNVLEDPE LREGIKEFSE
110 120 130 140 150
WPTIPQLYVN KEFIGGCDVI TSMARSGELA DLLEEAQALV PEEEEETKDR
Length:150
Mass (Da):16,931
Last modified:November 1, 1996 - v1
Checksum:iAD5FBABDF295DD04
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39205 Genomic DNA. Translation: AAB68306.1.
BK006949 Genomic DNA. Translation: DAA11371.1.
PIRiS60931.
RefSeqiNP_015266.1. NM_001183873.1.

Genome annotation databases

EnsemblFungiiYPL059W; YPL059W; YPL059W.
GeneIDi856048.
KEGGisce:YPL059W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39205 Genomic DNA. Translation: AAB68306.1.
BK006949 Genomic DNA. Translation: DAA11371.1.
PIRiS60931.
RefSeqiNP_015266.1. NM_001183873.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3GX8X-ray1.67A30-150[»]
ProteinModelPortaliQ02784.
SMRiQ02784. Positions 31-141.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36121. 30 interactions.
DIPiDIP-1352N.
IntActiQ02784. 4 interactions.
MINTiMINT-392130.

Proteomic databases

MaxQBiQ02784.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL059W; YPL059W; YPL059W.
GeneIDi856048.
KEGGisce:YPL059W.

Organism-specific databases

EuPathDBiFungiDB:YPL059W.
SGDiS000005980. GRX5.

Phylogenomic databases

GeneTreeiENSGT00550000075082.
HOGENOMiHOG000095211.
InParanoidiQ02784.
KOiK07390.
OMAiFNCLEDQ.
OrthoDBiEOG092C3TIP.

Enzyme and pathway databases

BioCyciYEAST:G3O-33970-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ02784.
PROiQ02784.

Family and domain databases

CDDicd03028. GRX_PICOT_like. 1 hit.
Gene3Di3.40.30.10. 1 hit.
InterProiIPR002109. Glutaredoxin.
IPR033658. GRX_PICOT_like.
IPR004480. Monothiol_GRX-rel.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR10293. PTHR10293. 1 hit.
PfamiPF00462. Glutaredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR00365. TIGR00365. 1 hit.
PROSITEiPS51354. GLUTAREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLRX5_YEAST
AccessioniPrimary (citable) accession number: Q02784
Secondary accession number(s): D6W3V5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6260 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.