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Protein

Monothiol glutaredoxin-5, mitochondrial

Gene

GRX5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Monothiol glutaredoxin involved in iron-sulfur biogenesis. Required for normal iron homeostasis. Protects cells against oxidative damage due to reactive oxygen species.1 Publication

Redox potential

E0 is -175 mV.1 Publication

Manual assertion based on experiment ini

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei52GlutathioneBy similarity1
Metal bindingi60Iron-sulfur (2Fe-2S); shared with dimeric partnerBy similarity1
Binding sitei104Glutathione; via amide nitrogen and carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cell redox homeostasis Source: InterPro
  • cellular response to oxidative stress Source: SGD
  • iron-sulfur cluster assembly Source: SGD
  • response to osmotic stress Source: SGD
Complete GO annotation...

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33970-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Monothiol glutaredoxin-5, mitochondrial
Gene namesi
Name:GRX5
Ordered Locus Names:YPL059W
ORF Names:LPE13W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL059W.
SGDiS000005980. GRX5.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 29Mitochondrion1 PublicationAdd BLAST29
ChainiPRO_000001163230 – 150Monothiol glutaredoxin-5, mitochondrialAdd BLAST121

Proteomic databases

MaxQBiQ02784.
PRIDEiQ02784.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi36121. 30 interactors.
DIPiDIP-1352N.
IntActiQ02784. 4 interactors.
MINTiMINT-392130.

Structurei

Secondary structure

1150
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi32 – 43Combined sources12
Beta strandi46 – 53Combined sources8
Beta strandi55 – 58Combined sources4
Helixi62 – 73Combined sources12
Helixi77 – 79Combined sources3
Beta strandi80 – 84Combined sources5
Helixi89 – 99Combined sources11
Beta strandi106 – 109Combined sources4
Beta strandi112 – 116Combined sources5
Helixi117 – 126Combined sources10
Helixi128 – 135Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GX8X-ray1.67A30-150[»]
ProteinModelPortaliQ02784.
SMRiQ02784.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ02784.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 140GlutaredoxinPROSITE-ProRule annotationAdd BLAST106

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni92 – 96Glutathione bindingBy similarity5
Regioni117 – 118Glutathione bindingBy similarity2

Sequence similaritiesi

Contains 1 glutaredoxin domain.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Transit peptide

Phylogenomic databases

GeneTreeiENSGT00550000075082.
HOGENOMiHOG000095211.
InParanoidiQ02784.
KOiK07390.
OMAiFNCLEDQ.
OrthoDBiEOG092C3TIP.

Family and domain databases

CDDicd03028. GRX_PICOT_like. 1 hit.
Gene3Di3.40.30.10. 1 hit.
InterProiIPR002109. Glutaredoxin.
IPR033658. GRX_PICOT-like.
IPR004480. Monothiol_GRX-rel.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR10293. PTHR10293. 1 hit.
PfamiPF00462. Glutaredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR00365. TIGR00365. 1 hit.
PROSITEiPS51354. GLUTAREDOXIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02784-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLPKFNPIR SFSPILRAKT LLRYQNRMYL STEIRKAIED AIESAPVVLF
60 70 80 90 100
MKGTPEFPKC GFSRATIGLL GNQGVDPAKF AAYNVLEDPE LREGIKEFSE
110 120 130 140 150
WPTIPQLYVN KEFIGGCDVI TSMARSGELA DLLEEAQALV PEEEEETKDR
Length:150
Mass (Da):16,931
Last modified:November 1, 1996 - v1
Checksum:iAD5FBABDF295DD04
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39205 Genomic DNA. Translation: AAB68306.1.
BK006949 Genomic DNA. Translation: DAA11371.1.
PIRiS60931.
RefSeqiNP_015266.1. NM_001183873.1.

Genome annotation databases

EnsemblFungiiYPL059W; YPL059W; YPL059W.
GeneIDi856048.
KEGGisce:YPL059W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39205 Genomic DNA. Translation: AAB68306.1.
BK006949 Genomic DNA. Translation: DAA11371.1.
PIRiS60931.
RefSeqiNP_015266.1. NM_001183873.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GX8X-ray1.67A30-150[»]
ProteinModelPortaliQ02784.
SMRiQ02784.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36121. 30 interactors.
DIPiDIP-1352N.
IntActiQ02784. 4 interactors.
MINTiMINT-392130.

Proteomic databases

MaxQBiQ02784.
PRIDEiQ02784.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL059W; YPL059W; YPL059W.
GeneIDi856048.
KEGGisce:YPL059W.

Organism-specific databases

EuPathDBiFungiDB:YPL059W.
SGDiS000005980. GRX5.

Phylogenomic databases

GeneTreeiENSGT00550000075082.
HOGENOMiHOG000095211.
InParanoidiQ02784.
KOiK07390.
OMAiFNCLEDQ.
OrthoDBiEOG092C3TIP.

Enzyme and pathway databases

BioCyciYEAST:G3O-33970-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ02784.
PROiQ02784.

Family and domain databases

CDDicd03028. GRX_PICOT_like. 1 hit.
Gene3Di3.40.30.10. 1 hit.
InterProiIPR002109. Glutaredoxin.
IPR033658. GRX_PICOT-like.
IPR004480. Monothiol_GRX-rel.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR10293. PTHR10293. 1 hit.
PfamiPF00462. Glutaredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR00365. TIGR00365. 1 hit.
PROSITEiPS51354. GLUTAREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLRX5_YEAST
AccessioniPrimary (citable) accession number: Q02784
Secondary accession number(s): D6W3V5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6260 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.