Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mitochondrial import inner membrane translocase subunit TIM50

Gene

TIM50

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Required to direct preproteins in transit and direct them to the channel protein TIM23, and possibly facilitates transfer of the translocating proteins from the TOM complex to the TIM23 complex. Does not act as a structural subunit of the TIM23 complex but plays a dynamic role in protein import. Has no phosphatase activity.3 Publications

GO - Molecular functioni

GO - Biological processi

  • protein import into mitochondrial matrix Source: SGD
  • protein targeting to mitochondrion Source: Reactome
  • regulation of mitochondrial membrane permeability Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33972-MONOMER.
ReactomeiR-SCE-1268020. Mitochondrial protein import.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM50
Gene namesi
Name:TIM50
Ordered Locus Names:YPL063W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL063W.
SGDiS000005984. TIM50.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini44 – 114Mitochondrial matrixSequence analysisAdd BLAST71
Transmembranei115 – 131HelicalSequence analysisAdd BLAST17
Topological domaini132 – 476Mitochondrial intermembraneSequence analysisAdd BLAST345

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 43Mitochondrion2 PublicationsAdd BLAST43
ChainiPRO_000004313944 – 476Mitochondrial import inner membrane translocase subunit TIM50Add BLAST433

Proteomic databases

MaxQBiQ02776.
PRIDEiQ02776.

Interactioni

Subunit structurei

Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21. Interacts directly with TIM23. Interacts with preproteins in transit.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-30302,EBI-30302
Aldh2P118843EBI-30302,EBI-916402From a different organism.
oliP008425EBI-30302,EBI-9084292From a different organism.
TIM21P5322010EBI-30302,EBI-23128
TIM23P3289712EBI-30302,EBI-9136

Protein-protein interaction databases

BioGridi36116. 31 interactors.
DIPiDIP-8544N.
IntActiQ02776. 21 interactors.
MINTiMINT-3374965.

Structurei

Secondary structure

1476
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni186 – 188Combined sources3
Beta strandi191 – 196Combined sources6
Turni200 – 202Combined sources3
Beta strandi203 – 207Combined sources5
Beta strandi209 – 211Combined sources3
Beta strandi213 – 217Combined sources5
Helixi221 – 228Combined sources8
Turni229 – 231Combined sources3
Beta strandi232 – 237Combined sources6
Helixi242 – 248Combined sources7
Helixi250 – 252Combined sources3
Turni253 – 255Combined sources3
Helixi256 – 258Combined sources3
Beta strandi261 – 263Combined sources3
Helixi265 – 267Combined sources3
Beta strandi268 – 271Combined sources4
Beta strandi274 – 276Combined sources3
Helixi279 – 281Combined sources3
Helixi286 – 288Combined sources3
Beta strandi289 – 294Combined sources6
Helixi296 – 299Combined sources4
Helixi303 – 305Combined sources3
Beta strandi306 – 308Combined sources3
Helixi321 – 323Combined sources3
Helixi324 – 332Combined sources9
Helixi339 – 342Combined sources4
Helixi348 – 350Combined sources3
Helixi351 – 354Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4QQFX-ray2.67A/B/C/D/E/F164-361[»]
ProteinModelPortaliQ02776.
SMRiQ02776.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini187 – 330FCP1 homologyPROSITE-ProRule annotationAdd BLAST144

Domaini

The mitochondrial intermembrane region is required for generation but not stabilization of the TIM23 complex.1 Publication
The FCP1 homology domain does not contain the canonical D-x-D-x-[TV] active site, suggesting that it probably does not display phosphatase activity.1 Publication

Sequence similaritiesi

Belongs to the TIM50 family.Curated
Contains 1 FCP1 homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000075036.
HOGENOMiHOG000183658.
InParanoidiQ02776.
KOiK17496.
OMAiGYLSQYY.
OrthoDBiEOG092C5CYS.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR004274. FCP1_dom.
IPR023214. HAD-like_dom.
IPR027111. Tim50.
[Graphical view]
PANTHERiPTHR12210:SF3. PTHR12210:SF3. 1 hit.
PfamiPF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
PROSITEiPS50969. FCP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02776-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSILRNSVR LNSRALRVVP SAANTLTSVQ ASRRLLTSYS SFLQKETKDD
60 70 80 90 100
KPKSILTDDM LFKAGVDVDE KGQGKNEETS GEGGEDKNEP SSKSEKSRRK
110 120 130 140 150
RQTSTDIKRE KYANWFYIFS LSALTGTAIY MARDWEPQES EELKKDIDNG
160 170 180 190 200
YTLSLMYKRF KARFNSMFTY FQEPPFPDLL PPPPPPPYQR PLTLVITLED
210 220 230 240 250
FLVHSEWSQK HGWRTAKRPG ADYFLGYLSQ YYEIVLFSSN YMMYSDKIAE
260 270 280 290 300
KLDPIHAFVS YNLFKEHCVY KDGVHIKDLS KLNRDLSKVI IIDTDPNSYK
310 320 330 340 350
LQPENAIPME PWNGEADDKL VRLIPFLEYL ATQQTKDVRP ILNSFEDKKN
360 370 380 390 400
LAEEFDHRVK KLKDKFYGDH KSGGNWAMTA LGLGNSLGGS TKFPLDLIHE
410 420 430 440 450
EGQKNYLMFM KMIEEEKEKI RIQQEQMGGQ TFTLKDYVEG NLPSPEEQMK
460 470
IQLEKQKEVD ALFEEEKKKK KIAESK
Length:476
Mass (Da):55,099
Last modified:November 1, 1996 - v1
Checksum:i778D54F5C4ED27C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39205 Genomic DNA. Translation: AAB68302.1.
BK006949 Genomic DNA. Translation: DAA11368.1.
PIRiS60927.
RefSeqiNP_015262.1. NM_001183877.1.

Genome annotation databases

EnsemblFungiiYPL063W; YPL063W; YPL063W.
GeneIDi856042.
KEGGisce:YPL063W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39205 Genomic DNA. Translation: AAB68302.1.
BK006949 Genomic DNA. Translation: DAA11368.1.
PIRiS60927.
RefSeqiNP_015262.1. NM_001183877.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4QQFX-ray2.67A/B/C/D/E/F164-361[»]
ProteinModelPortaliQ02776.
SMRiQ02776.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36116. 31 interactors.
DIPiDIP-8544N.
IntActiQ02776. 21 interactors.
MINTiMINT-3374965.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Proteomic databases

MaxQBiQ02776.
PRIDEiQ02776.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL063W; YPL063W; YPL063W.
GeneIDi856042.
KEGGisce:YPL063W.

Organism-specific databases

EuPathDBiFungiDB:YPL063W.
SGDiS000005984. TIM50.

Phylogenomic databases

GeneTreeiENSGT00550000075036.
HOGENOMiHOG000183658.
InParanoidiQ02776.
KOiK17496.
OMAiGYLSQYY.
OrthoDBiEOG092C5CYS.

Enzyme and pathway databases

BioCyciYEAST:G3O-33972-MONOMER.
ReactomeiR-SCE-1268020. Mitochondrial protein import.

Miscellaneous databases

PROiQ02776.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR004274. FCP1_dom.
IPR023214. HAD-like_dom.
IPR027111. Tim50.
[Graphical view]
PANTHERiPTHR12210:SF3. PTHR12210:SF3. 1 hit.
PfamiPF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
PROSITEiPS50969. FCP1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTIM50_YEAST
AccessioniPrimary (citable) accession number: Q02776
Secondary accession number(s): D6W3V2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2400 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.