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Protein

Mitochondrial import inner membrane translocase subunit TIM50

Gene

TIM50

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Required to direct preproteins in transit and direct them to the channel protein TIM23, and possibly facilitates transfer of the translocating proteins from the TOM complex to the TIM23 complex. Does not act as a structural subunit of the TIM23 complex but plays a dynamic role in protein import. Has no phosphatase activity.3 Publications

Miscellaneous

Present with 2400 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • mitochondrion targeting sequence binding Source: SGD
  • phosphoprotein phosphatase activity Source: GO_Central

GO - Biological processi

  • protein import into mitochondrial matrix Source: SGD
  • regulation of mitochondrial membrane permeability Source: SGD

Keywordsi

Biological processProtein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33972-MONOMER

Protein family/group databases

TCDBi3.A.8.1.1 the mitochondrial protein translocase (mpt) family

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM50
Gene namesi
Name:TIM50
Ordered Locus Names:YPL063W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL063W
SGDiS000005984 TIM50

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini44 – 114Mitochondrial matrixSequence analysisAdd BLAST71
Transmembranei115 – 131HelicalSequence analysisAdd BLAST17
Topological domaini132 – 476Mitochondrial intermembraneSequence analysisAdd BLAST345

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 43Mitochondrion2 PublicationsAdd BLAST43
ChainiPRO_000004313944 – 476Mitochondrial import inner membrane translocase subunit TIM50Add BLAST433

Proteomic databases

MaxQBiQ02776
PaxDbiQ02776
PRIDEiQ02776

Interactioni

Subunit structurei

Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21. Interacts directly with TIM23. Interacts with preproteins in transit.3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi36116, 229 interactors
DIPiDIP-8544N
IntActiQ02776, 21 interactors
MINTiQ02776
STRINGi4932.YPL063W

Structurei

Secondary structure

1476
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni186 – 188Combined sources3
Beta strandi191 – 196Combined sources6
Turni200 – 202Combined sources3
Beta strandi203 – 207Combined sources5
Beta strandi209 – 211Combined sources3
Beta strandi213 – 217Combined sources5
Helixi221 – 228Combined sources8
Turni229 – 231Combined sources3
Beta strandi232 – 237Combined sources6
Helixi242 – 248Combined sources7
Helixi250 – 252Combined sources3
Turni253 – 255Combined sources3
Helixi256 – 258Combined sources3
Beta strandi261 – 263Combined sources3
Helixi265 – 267Combined sources3
Beta strandi268 – 271Combined sources4
Beta strandi274 – 276Combined sources3
Helixi279 – 281Combined sources3
Helixi286 – 288Combined sources3
Beta strandi289 – 294Combined sources6
Helixi296 – 299Combined sources4
Helixi303 – 305Combined sources3
Beta strandi306 – 308Combined sources3
Helixi321 – 323Combined sources3
Helixi324 – 332Combined sources9
Helixi339 – 342Combined sources4
Helixi348 – 350Combined sources3
Helixi351 – 354Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4QQFX-ray2.67A/B/C/D/E/F164-361[»]
ProteinModelPortaliQ02776
SMRiQ02776
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini187 – 330FCP1 homologyPROSITE-ProRule annotationAdd BLAST144

Domaini

The mitochondrial intermembrane region is required for generation but not stabilization of the TIM23 complex.1 Publication
The FCP1 homology domain does not contain the canonical D-x-D-x-[TV] active site, suggesting that it probably does not display phosphatase activity.1 Publication

Sequence similaritiesi

Belongs to the TIM50 family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000075036
HOGENOMiHOG000183658
InParanoidiQ02776
KOiK17496
OMAiENGEKWL
OrthoDBiEOG092C5CYS

Family and domain databases

Gene3Di3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR004274 FCP1_dom
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR027111 Tim50
PANTHERiPTHR12210:SF3 PTHR12210:SF3, 1 hit
PfamiView protein in Pfam
PF03031 NIF, 1 hit
SMARTiView protein in SMART
SM00577 CPDc, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
PROSITEiView protein in PROSITE
PS50969 FCP1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02776-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSILRNSVR LNSRALRVVP SAANTLTSVQ ASRRLLTSYS SFLQKETKDD
60 70 80 90 100
KPKSILTDDM LFKAGVDVDE KGQGKNEETS GEGGEDKNEP SSKSEKSRRK
110 120 130 140 150
RQTSTDIKRE KYANWFYIFS LSALTGTAIY MARDWEPQES EELKKDIDNG
160 170 180 190 200
YTLSLMYKRF KARFNSMFTY FQEPPFPDLL PPPPPPPYQR PLTLVITLED
210 220 230 240 250
FLVHSEWSQK HGWRTAKRPG ADYFLGYLSQ YYEIVLFSSN YMMYSDKIAE
260 270 280 290 300
KLDPIHAFVS YNLFKEHCVY KDGVHIKDLS KLNRDLSKVI IIDTDPNSYK
310 320 330 340 350
LQPENAIPME PWNGEADDKL VRLIPFLEYL ATQQTKDVRP ILNSFEDKKN
360 370 380 390 400
LAEEFDHRVK KLKDKFYGDH KSGGNWAMTA LGLGNSLGGS TKFPLDLIHE
410 420 430 440 450
EGQKNYLMFM KMIEEEKEKI RIQQEQMGGQ TFTLKDYVEG NLPSPEEQMK
460 470
IQLEKQKEVD ALFEEEKKKK KIAESK
Length:476
Mass (Da):55,099
Last modified:November 1, 1996 - v1
Checksum:i778D54F5C4ED27C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39205 Genomic DNA Translation: AAB68302.1
BK006949 Genomic DNA Translation: DAA11368.1
PIRiS60927
RefSeqiNP_015262.1, NM_001183877.1

Genome annotation databases

EnsemblFungiiYPL063W; YPL063W; YPL063W
GeneIDi856042
KEGGisce:YPL063W

Similar proteinsi

Entry informationi

Entry nameiTIM50_YEAST
AccessioniPrimary (citable) accession number: Q02776
Secondary accession number(s): D6W3V2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

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