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Protein

Vacuolar protein sorting-associated protein 28

Gene

VPS28

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for normal endocytic and biosynthetic traffic to the yeast vacuole.

Miscellaneous

Present with 1420 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • protein-containing complex binding Source: SGD

GO - Biological processi

  • ATP export Source: SGD
  • protein targeting to membrane Source: SGD
  • protein targeting to vacuole Source: SGD
  • protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: GO_Central
  • ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33974-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 28
Alternative name(s):
ESCRT-I complex subunit VPS28
Gene namesi
Name:VPS28
Synonyms:VPT28
Ordered Locus Names:YPL065W
ORF Names:LPE5W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL065W
SGDiS000005986 VPS28

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi40L → D: Abolishes ESCRT-I complex assembly; class E phenotype (malformed late MVB); when associated with D-44. 1 Publication1
Mutagenesisi44Y → D: Abolishes ESCRT-I complex assembly; class E phenotype (malformed late MVB); when associated with D-40. 1 Publication1
Mutagenesisi228 – 231FDLE → ASLA: Abolishes interaction with VPS20. 1 Publication4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001209581 – 242Vacuolar protein sorting-associated protein 28Add BLAST242

Proteomic databases

MaxQBiQ02767
PaxDbiQ02767
PRIDEiQ02767

Interactioni

Subunit structurei

Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of STP22, VPS28, SRN2 and MVB12 in a 1:1:1:1 stoichiometry. Self-associates. Interacts with VPS27; the interaction mediates the association with the ESCRT-0 complex. Interacts with VPS20; the interaction mediates the association with the ESCRT-III complex.4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36114, 108 interactors
DIPiDIP-5828N
IntActiQ02767, 6 interactors
MINTiQ02767
STRINGi4932.YPL065W

Structurei

Secondary structure

1242
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 12Combined sources3
Helixi14 – 17Combined sources4
Beta strandi27 – 29Combined sources3
Helixi31 – 58Combined sources28
Helixi64 – 82Combined sources19
Turni85 – 88Combined sources4
Helixi89 – 103Combined sources15
Helixi109 – 117Combined sources9
Helixi150 – 168Combined sources19
Helixi174 – 191Combined sources18
Helixi199 – 210Combined sources12
Helixi220 – 240Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CAZX-ray3.60B/E1-147[»]
2F66X-ray2.80B/E13-125[»]
2F6MX-ray2.10B/D13-118[»]
2G3KX-ray3.05A/B/C/D/E/F/G148-241[»]
2J9UX-ray2.00A/C148-242[»]
2J9VX-ray2.00A148-242[»]
2P22X-ray2.70B1-118[»]
ProteinModelPortaliQ02767
SMRiQ02767
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ02767

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 125VPS28 N-terminalPROSITE-ProRule annotationAdd BLAST115
Domaini148 – 242VPS28 C-terminalPROSITE-ProRule annotationAdd BLAST95

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni148 – 242Interaction with VSP36 and VPS201 PublicationAdd BLAST95

Sequence similaritiesi

Belongs to the VPS28 family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000007486
HOGENOMiHOG000203818
InParanoidiQ02767
KOiK12184
OMAiKYRLDCP
OrthoDBiEOG092C4CZF

Family and domain databases

Gene3Di1.20.120.1130, 1 hit
1.20.1440.200, 1 hit
InterProiView protein in InterPro
IPR037202 ESCRT_assembly_dom
IPR007143 Vps28
IPR017899 VPS28_C
IPR037206 VPS28_C_sf
IPR017898 VPS28_N
IPR038358 VPS28_N_sf
PANTHERiPTHR12937 PTHR12937, 1 hit
PfamiView protein in Pfam
PF03997 VPS28, 1 hit
PIRSFiPIRSF017535 VPS28, 1 hit
SUPFAMiSSF140111 SSF140111, 1 hit
SSF140427 SSF140427, 1 hit
PROSITEiView protein in PROSITE
PS51310 VPS28_C, 1 hit
PS51313 VPS28_N, 1 hit

Sequencei

Sequence statusi: Complete.

Q02767-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKHNIKLNQ NQDISQLFHD EVPLFDNSIT SKDKEVIETL SEIYSIVITL
60 70 80 90 100
DHVEKAYLKD SIDDTQYTNT VDKLLKQFKV YLNSQNKEEI NKHFQSIEAF
110 120 130 140 150
CDTYNITASN AITRLERGIP ITAEHAISTT TSAPSGDNKQ SSSSDKKFNA
160 170 180 190 200
KYVAEATGNF ITVMDALKLN YNAKDQLHPL LAELLISINR VTRDDFENRS
210 220 230 240
KLIDWIVRIN KLSIGDTLTE TQIRELLFDL ELAYKSFYAL LD
Length:242
Mass (Da):27,702
Last modified:November 1, 1996 - v1
Checksum:iDE091D3F60C9D132
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti193R → K in AAT92809 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U50630 Genomic DNA Translation: AAB40936.1
U39205 Genomic DNA Translation: AAB68300.1
AY692790 Genomic DNA Translation: AAT92809.1
BK006949 Genomic DNA Translation: DAA11366.1
PIRiS60925
RefSeqiNP_015260.1, NM_001183879.1

Genome annotation databases

EnsemblFungiiYPL065W; YPL065W; YPL065W
GeneIDi856040
KEGGisce:YPL065W

Similar proteinsi

Entry informationi

Entry nameiVPS28_YEAST
AccessioniPrimary (citable) accession number: Q02767
Secondary accession number(s): D6W3V0, E9P8X2, P87328
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: April 25, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health