Reviewed, UniProtKB/Swiss-Prot Q02762 (UCRI_RHOSH)

Names and origin

Protein names

Recommended name:
    Ubiquinol-cytochrome c reductase iron-sulfur subunit
    EC=1.10.2.2
Alternative name(s):
    Rieske iron-sulfur protein
      Short name=RISP

Gene names

Name:	petA
Synonyms:	fbcF

Organism

Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides)

Taxonomic identifier

1063 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
Catalytic activity	QH2 + 2 ferricytochrome c = Q + 2 ferrocytochrome c + 2 H+.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity.
Subunit structure	The main subunits of complex b-c1 are: cytochrome b, cytochrome c1 and the Rieske protein.
Subcellular location	Cell membrane; Single-pass membrane protein.
Miscellaneous	The Rieske protein is a high potential 2Fe-2S protein.
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	3D-structure
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 187

187

Ubiquinol-cytochrome c reductase iron-sulfur subunit

PRO_0000127764

Regions

Transmembrane

15 – 35

21

Potential

Domain

89 – 185

97

Rieske

Sites

Metal binding

129

1

Iron-sulfur (2Fe-2S) By similarity

Metal binding

131

1

Iron-sulfur (2Fe-2S); via pros nitrogen By similarity

Metal binding

149

1

Iron-sulfur (2Fe-2S) By similarity

Metal binding

152

1

Iron-sulfur (2Fe-2S); via pros nitrogen By similarity

Amino acid modifications

Disulfide bond

134 ↔ 151

By similarity

Secondary structure

1

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187

Helix Strand Turn

Details...

Helix

10 – 36

27

Turn

42 – 44

3

Beta strand

50 – 53

4

Beta strand

61 – 67

7

Beta strand

70 – 76

7

Helix

79 – 86

8

Helix

90 – 92

3

Helix

111 – 114

4

Beta strand

115 – 117

3

Beta strand

122 – 126

5

Turn

130 – 132

3

Beta strand

137 – 141

5

Turn

142 – 145

4

Beta strand

146 – 149

4

Turn

150 – 153

4

Beta strand

154 – 156

3

Beta strand

162 – 166

5

Beta strand

175 – 186

12

Sequences

	Sequence		Length	Mass (Da)
	Q02762-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 575B47EEEECD74D2	FASTA	187	19,910
	10 20 30 40 50 60 MSNAEDHAGT RRDFLYYATA GAGAVATGAA VWPLINQMNP SADVQALASI FVDVSSVEPG 70 80 90 100 110 120 VQLTVKFLGK PIFIRRRTEA DIELGRSVQL GQLVDTNARN ANIDAGAEAT DQNRTLDEAG 130 140 150 160 170 180 EWLVMWGVCT HLGCVPIGGV SGDFGGWFCP CHGSHYDSAG RIRKGPAPEN LPIPLAKFID ETTIQLG

References

[1]	"Cloning and DNA sequencing of the fbc operon encoding the cytochrome bc1 complex from Rhodobacter sphaeroides. Characterization of fbc deletion mutants and complementation by a site-specific mutational variant." Yun C.-H., Beci R., Crofts A.R., Kaplan S., Gennis R.B. Eur. J. Biochem. 194:399-411(1990) [PubMed: 2176595] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"fbc operon, encoding the Rieske Fe-S protein cytochrome b, and cytochrome c1 apoproteins previously described from Rhodopseudomonas sphaeroides, is from Rhodopseudomonas capsulata." Davidson E., Daldal F. J. Mol. Biol. 195:25-29(1987) [PubMed: 2821272] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-95.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X56157 Genomic DNA. Translation: CAA39623.1.
M18577 Genomic DNA. Translation: AAA26150.1.

PIR

S13868.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2FYN	X-ray	3.20	C/F/I/L/O/R	1-187	[»]
2NUK	X-ray	1.20	A	47-187	[»]
2NUM	X-ray	1.50	A	47-187	[»]
2NVE	X-ray	1.50	A	47-187	[»]
2NVF	X-ray	1.50	A	47-187	[»]
2NVG	X-ray	1.35	A	47-187	[»]
2NWF	X-ray	1.10	A	47-187	[»]
2QJK	X-ray	3.10	C/F/I/L/O/R	9-187	[»]
2QJP	X-ray	2.60	C/F/I/L	9-187	[»]
2QJY	X-ray	2.40	C/F/I/L/O/R	1-187	[»]

ModBase

Search...

Protein family/group databases

TCDB

3.E.2.1.1. photosynthetic reaction center (PRC) family.

Enzyme and pathway databases

BRENDA

1.10.2.2. 2796.

Family and domain databases

InterPro

IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR006311. Tat.
IPR017909. Twin_arg_translocation_Tat.
IPR019470. Ubiq_cytC_Rdtase_Fe-S_su_TAT.
IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su.
[Graphical view]

Gene3D

G3DSA:2.102.10.10. Rieske_reg. 1 hit.

PANTHER

PTHR10134. Rieske. 1 hit.

Pfam

PF00355. Rieske. 1 hit.
PF10399. UCR_Fe-S_N. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01416. Rieske_proteo. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.

PROSITE

PS51296. RIESKE. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

UCRI_RHOSH

Accession

Primary (citable) accession number: Q02762
Secondary accession number(s): P72327

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)