Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Arachidonate 15-lipoxygenase

Gene

Alox15

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid into 12-hydroperoxyeicosatetraenoic acid/12-HPETE and 15-hydroperoxyeicosatetraenoic acid/15-HPETE. Also converts linoleic acid to 13-hydroperoxyoctadecadienoic acid. May also act on (12S)-hydroperoxyeicosatetraenoic acid/(12S)-HPETE to produce hepoxilin A3. Probably plays an important role in the immune and inflammatory responses. Through the oxygenation of membrane-bound phosphatidylethanolamine in macrophages may favor clearance of apoptotic cells during inflammation by resident macrophages and prevent an autoimmune response associated with the clearance of apoptotic cells by inflammatory monocytes. In parallel, may regulate actin polymerization which is crucial for several biological processes, including macrophage function. May also regulate macrophage function through regulation of the peroxisome proliferator activated receptor signaling pathway. Finally, it is also involved in the cellular response to IL13/interleukin-13. In addition to its role in the immune and inflammatory responses, may play a role in epithelial wound healing in the cornea maybe through production of lipoxin A4. May also play a role in endoplasmic reticulum stress response and the regulation of bone mass.4 Publications

Catalytic activityi

Arachidonate + O2 = (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate.2 Publications
Arachidonate + O2 = (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate.2 Publications

Cofactori

Fe cationNote: Binds 1 Fe cation per subunit.

Pathwayi: hydroperoxy eicosatetraenoic acid biosynthesis

This protein is involved in the pathway hydroperoxy eicosatetraenoic acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway hydroperoxy eicosatetraenoic acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi361Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi366Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi541Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi545Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi663Iron; via carboxylate; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

  • arachidonate 12-lipoxygenase activity Source: RGD
  • arachidonate 15-lipoxygenase activity Source: RGD
  • hepoxilin A3 synthase activity Source: RGD
  • hepoxilin-epoxide hydrolase activity Source: UniProtKB
  • iron ion binding Source: UniProtKB
  • phosphatidylinositol-4,5-bisphosphate binding Source: UniProtKB
  • potassium channel inhibitor activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

Calcium, Iron, Lipid-binding, Metal-binding

Enzyme and pathway databases

BRENDAi1.13.11.31. 5301.
ReactomeiR-RNO-2142691. Synthesis of Leukotrienes (LT) and Eoxins (EX).
R-RNO-2142712. Synthesis of 12-eicosatetraenoic acid derivatives.
R-RNO-2142770. Synthesis of 15-eicosatetraenoic acid derivatives.
UniPathwayiUPA00881.

Chemistry databases

SwissLipidsiSLP:000001609.

Names & Taxonomyi

Protein namesi
Recommended name:
Arachidonate 15-lipoxygenase (EC:1.13.11.332 Publications)
Short name:
15-LOX
Alternative name(s):
12/15-lipoxygenase
Arachidonate 12-lipoxygenase, leukocyte-type1 Publication (EC:1.13.11.312 Publications)
Short name:
12-LOX
Arachidonate omega-6 lipoxygenase
Gene namesi
Name:Alox15
Synonyms:Alox12l
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi70493. Alox15.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Lipid droplet, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2741.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002206872 – 663Arachidonate 15-lipoxygenaseAdd BLAST662

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei149PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ02759.
PRIDEiQ02759.

PTM databases

iPTMnetiQ02759.
PhosphoSitePlusiQ02759.

Expressioni

Tissue specificityi

Detected in leukocytes, lung and aorta.1 Publication

Gene expression databases

BgeeiENSRNOG00000019183.
GenevisibleiQ02759. RN.

Interactioni

Subunit structurei

Interacts with PEBP1; in response to IL13/interleukin-13, prevents the interaction of PEBP1 with RAF1 to activate the ERK signaling cascade.By similarity

Protein-protein interaction databases

IntActiQ02759. 1 interactor.
STRINGi10116.ENSRNOP00000026038.

Chemistry databases

BindingDBiQ02759.

Structurei

3D structure databases

SMRiQ02759.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 115PLATPROSITE-ProRule annotationAdd BLAST114
Domaini116 – 663LipoxygenasePROSITE-ProRule annotationAdd BLAST548

Domaini

The PLAT domain can bind calcium ions; this promotes association with membranes.By similarity

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated
Contains 1 lipoxygenase domain.PROSITE-ProRule annotation
Contains 1 PLAT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IKAN. Eukaryota.
ENOG410YN4N. LUCA.
GeneTreeiENSGT00550000074415.
HOGENOMiHOG000234358.
HOVERGENiHBG005150.
InParanoidiQ02759.
KOiK00460.
OMAiLTCWKDL.
OrthoDBiEOG091G04A4.
TreeFamiTF105320.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
InterProiIPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001885. LipOase_mml.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 1 hit.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00467. MAMLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02759-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVYRIRVST GDSKYAGSNN EVYLWLVGQH GEASLGKLLR PCRDSEAEFK
60 70 80 90 100
VDVSEYLGPL LFVRVQKWHY LTDDAWFCNW ISVKGPGDQG SEYMFPCYRW
110 120 130 140 150
VQGRSILSLP EGTGCTVVED SQGLFRKHRE EELEERRSLY RWGNWKDGSI
160 170 180 190 200
LNVAAASISD LPVDQRFRED KRIEFEASQV IGVMDTVVNF PINTVTCWKS
210 220 230 240 250
LDDFNCVFKS GHTKMAERVR NSWKEDAFFG YQFLNGANPM VLKRSTCLPA
260 270 280 290 300
RLVFPPGMEK LQAQLNKELQ KGTLFEADFF LLDGIKANVI LCSQQYLAAP
310 320 330 340 350
LVMLKLMPDG QLLPIAIQLE LPKTGSTPPP IFTPSDPPMD WLLAKCWVRS
360 370 380 390 400
SDLQLHELQA HLLRGHLMAE VFAVATMRCL PSVHPVFKLL VPHLLYTMEI
410 420 430 440 450
NVRARSDLIS ERGFFDKAMS TGGGGHLDLL KQAGAFLTYC SLCPPDDLAE
460 470 480 490 500
RGLLDIETCF YAKDALRLWQ IMNRYVVGMF NLHYKTDKAV QDDYELQSWC
510 520 530 540 550
REITDIGLQG AQDRGFPTSL QSRAQACYFI TMCIFTCTAQ HSSVHLGQLD
560 570 580 590 600
WFYWVPNAPC TMRLPPPTTK EATMEKLMAT LPNPNQSTLQ INVVWLLGRR
610 620 630 640 650
QAVMVPLGQH SEEHFPNPEA KAVLKKFREE LAALDKEIEI RNKSLDIPYE
660
YLRPSMVENS VAI
Length:663
Mass (Da):75,378
Last modified:October 16, 2013 - v3
Checksum:iC732D3EB22CA0411
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti55E → G in AAB30132 (PubMed:8117750).Curated1
Sequence conflicti371V → L in AAA41532 (PubMed:8444196).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06040 mRNA. Translation: AAA41532.1.
S69383 mRNA. Translation: AAB30132.1.
AABR06064408 Genomic DNA. No translation available.
PIRiI52462.
S30051.
RefSeqiNP_112272.2. NM_031010.2.
UniGeneiRn.11318.

Genome annotation databases

EnsembliENSRNOT00000026038; ENSRNOP00000026038; ENSRNOG00000019183.
GeneIDi81639.
KEGGirno:81639.
UCSCiRGD:70493. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06040 mRNA. Translation: AAA41532.1.
S69383 mRNA. Translation: AAB30132.1.
AABR06064408 Genomic DNA. No translation available.
PIRiI52462.
S30051.
RefSeqiNP_112272.2. NM_031010.2.
UniGeneiRn.11318.

3D structure databases

SMRiQ02759.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ02759. 1 interactor.
STRINGi10116.ENSRNOP00000026038.

Chemistry databases

BindingDBiQ02759.
ChEMBLiCHEMBL2741.
SwissLipidsiSLP:000001609.

PTM databases

iPTMnetiQ02759.
PhosphoSitePlusiQ02759.

Proteomic databases

PaxDbiQ02759.
PRIDEiQ02759.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000026038; ENSRNOP00000026038; ENSRNOG00000019183.
GeneIDi81639.
KEGGirno:81639.
UCSCiRGD:70493. rat.

Organism-specific databases

CTDi246.
RGDi70493. Alox15.

Phylogenomic databases

eggNOGiENOG410IKAN. Eukaryota.
ENOG410YN4N. LUCA.
GeneTreeiENSGT00550000074415.
HOGENOMiHOG000234358.
HOVERGENiHBG005150.
InParanoidiQ02759.
KOiK00460.
OMAiLTCWKDL.
OrthoDBiEOG091G04A4.
TreeFamiTF105320.

Enzyme and pathway databases

UniPathwayiUPA00881.
BRENDAi1.13.11.31. 5301.
ReactomeiR-RNO-2142691. Synthesis of Leukotrienes (LT) and Eoxins (EX).
R-RNO-2142712. Synthesis of 12-eicosatetraenoic acid derivatives.
R-RNO-2142770. Synthesis of 15-eicosatetraenoic acid derivatives.

Miscellaneous databases

PROiQ02759.

Gene expression databases

BgeeiENSRNOG00000019183.
GenevisibleiQ02759. RN.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
InterProiIPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001885. LipOase_mml.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 1 hit.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00467. MAMLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLOX15_RAT
AccessioniPrimary (citable) accession number: Q02759
Secondary accession number(s): F1MA51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: October 16, 2013
Last modified: November 2, 2016
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.