Q02753 (RL21A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
60S ribosomal protein L21-A

Gene names

Name:	RPL21A
Synonyms:	URP1
Ordered Locus Names:	YBR191W
ORF Names:	YBR1401

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	160 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.6
Subcellular location	Cytoplasm Ref.8.
Miscellaneous	There are 2 genes for L21 in yeast.
Sequence similarities	Belongs to the ribosomal protein L21e family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Traceable author statement Ref.6. Source: SGD
Cellular_component	cytosolic large ribosomal subunit Traceable author statement Ref.6. Source: SGD
Molecular_function	structural constituent of ribosome Traceable author statement Ref.6. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.7
	Chain	2 – 160	159	60S ribosomal protein L21-A		PRO_0000149682
Amino acid modifications
	Modified residue	71	1	Phosphoserine Ref.9
	Modified residue	142	1	Phosphoserine Ref.9
	Modified residue	143	1	Phosphothreonine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	Q02753 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 45286521C8473A72	FASTA	160	18,242
	10 20 30 40 50 60 MGKSHGYRSR TRYMFQRDFR KHGAVHLSTY LKVYKVGDIV DIKANGSIQK GMPHKFYQGK 70 80 90 100 110 120 TGVVYNVTKS SVGVIINKMV GNRYLEKRLN LRVEHIKHSK CRQEFLERVK ANAAKRAEAK 130 140 150 160 AQGVAVQLKR QPAQPRESRI VSTEGNVPQT LAPVPYETFI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast single copy gene URP1 is a homolog of rat ribosomal protein gene L21." Jank B., Waldherr M., Schweyen R.J. Curr. Genet. 23:15-18(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"A 12.5 kb fragment of the yeast chromosome II contains two adjacent genes encoding ribosomal proteins and six putative new genes, one of which encodes a putative transcriptional factor." Demolis N., Jacquet M., Mallet L. Yeast 10:1511-1525(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-39. Strain: ATCC 204508 / S288c.
[5]	"RIM2, MSI1 and PGI1 are located within an 8 kb segment of Saccharomyces cerevisiae chromosome II, which also contains the putative ribosomal gene L21 and a new putative essential gene with a leucine zipper motif." Demolis N., Mallet L., Bussereau F., Jacquet M. Yeast 9:645-659(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 40-160. Strain: ATCC 204508 / S288c.
[6]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[7]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-71; SER-142 AND THR-143, MASS SPECTROMETRY.
[10]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 4-100, ELECTRON MICROSCOPY.
[11]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 2-100, ELECTRON MICROSCOPY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M86408 Genomic DNA. Translation: AAA35202.1.
Z36059 Genomic DNA. Translation: CAA85153.1.
Z21487 Genomic DNA. Translation: CAA79677.1.
U02073 Genomic DNA. Translation: AAB60284.1.
BK006936 Genomic DNA. Translation: DAA07305.1.

PIR

S28921.

RefSeq

NP_009750.1. NM_001178539.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5Y	model	-	Q	4-100	[»]
1S1I	electron microscopy	11.70	Q	1-100	[»]
3IZS	electron microscopy	-	U	1-160	[»]
3O58	X-ray	4.00	T	1-160	[»]
3O5H	X-ray	4.00	T	1-160	[»]
3U5E	X-ray	3.00	T	1-160	[»]
3U5I	X-ray	3.00	T	1-160	[»]
4B6A	electron microscopy	8.10	T	1-160	[»]

ProteinModelPortal

Q02753.

SMR

Q02753. Positions 2-160.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q02753. 1 interaction.

MINT

MINT-1324156.

STRING

4932.YBR191W.

Proteomic databases

PeptideAtlas

Q02753.

PRIDE

Q02753.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YBR191W; YBR191W; YBR191W.

GeneID

852489.

KEGG

sce:YBR191W.

Organism-specific databases

CYGD

YBR191w.

SGD

S000000395. RPL21A.

Phylogenomic databases

GeneTree

ENSGT00390000001293.

HOGENOM

HOG000194513.

KO

K02889.

OMA

GVIVYKV.

OrthoDB

EOG412QFT.

Gene expression databases

Genevestigator

Q02753.

GermOnline

YBR191W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR001147. Ribosomal_L21e.
IPR018259. Ribosomal_L21e_CS.
IPR008991. Translation_prot_SH3-like.
[Graphical view]

PANTHER

PTHR20981. PTHR20981. 1 hit.

Pfam

PF01157. Ribosomal_L21e. 1 hit.
[Graphical view]

SUPFAM

SSF50104. Transl_SH3_like. 1 hit.

PROSITE

PS01171. RIBOSOMAL_L21E. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q02753.

NextBio

971474.

Entry information

Entry name

RL21A_YEAST

Accession

Primary (citable) accession number: Q02753
Secondary accession number(s): D6VQI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	April 3, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program