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Protein

Eukaryotic initiation factor 4A

Gene

eIF4A

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. Involved in germ cell formation.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi74 – 81ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: GO_Central
  • RNA helicase activity Source: FlyBase
  • translation initiation factor activity Source: FlyBase

GO - Biological processi

  • female germ-line stem cell asymmetric division Source: FlyBase
  • germ-line stem cell population maintenance Source: FlyBase
  • imaginal disc growth Source: FlyBase
  • instar larval development Source: FlyBase
  • mitotic cell cycle Source: FlyBase
  • oogenesis Source: FlyBase
  • pole cell formation Source: FlyBase
  • regulation of alternative mRNA splicing, via spliceosome Source: FlyBase
  • RNA secondary structure unwinding Source: GO_Central
  • translational initiation Source: FlyBase

Keywordsi

Molecular functionHelicase, Hydrolase, Initiation factor, RNA-binding
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-1169408 ISG15 antiviral mechanism
R-DME-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-DME-72649 Translation initiation complex formation
R-DME-72662 Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S
R-DME-72702 Ribosomal scanning and start codon recognition

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic initiation factor 4A (EC:3.6.4.13)
Alternative name(s):
ATP-dependent RNA helicase eIF4A
Gene namesi
Name:eIF4A1 PublicationImported
Synonyms:eIF-4a1 Publication, l(2L)162
ORF Names:CG9075Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0001942 eIF4A

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000549471 – 403Eukaryotic initiation factor 4AAdd BLAST403

Proteomic databases

PaxDbiQ02748
PRIDEiQ02748

Expressioni

Gene expression databases

BgeeiFBgn0001942
ExpressionAtlasiQ02748 baseline and differential
GenevisibleiQ02748 DM

Interactioni

Subunit structurei

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least eIF4A, eIF4E1 and eIF4G1. Interacts with tud and vas.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
bamP227454EBI-85570,EBI-88504

Protein-protein interaction databases

BioGridi60000, 56 interactors
DIPiDIP-18113N
IntActiQ02748, 24 interactors
MINTiQ02748
STRINGi7227.FBpp0297911

Structurei

3D structure databases

ProteinModelPortaliQ02748
SMRiQ02748
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini61 – 231Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST171
Domaini242 – 403Helicase C-terminalPROSITE-ProRule annotationAdd BLAST162

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi30 – 58Q motifAdd BLAST29
Motifi179 – 182DEAD box4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0327 Eukaryota
COG0513 LUCA
GeneTreeiENSGT00530000062880
InParanoidiQ02748
KOiK03257
OMAiRNPIRIL
OrthoDBiEOG091G07OI
PhylomeDBiQ02748

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR037583 EIF4A
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR000629 RNA-helicase_DEAD-box_CS
IPR014014 RNA_helicase_DEAD_Q_motif
PANTHERiPTHR24031:SF226 PTHR24031:SF226, 1 hit
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00039 DEAD_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequencei

Sequence statusi: Complete.

Q02748-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDRNEIPQD GPASMEPEGV IESTWHEVYD NFDDMNLREE LLRGIYGYGF
60 70 80 90 100
EKPSAIQQRA IIPCVRGRDV IAQAQSGTGK TATFSIAILQ QIDTSIRECQ
110 120 130 140 150
ALILAPTREL ATQIQRVVMA LGEYMKVHSH ACIGGTNVRE DARILESGCH
160 170 180 190 200
VVVGTPGRVY DMINRKVLRT QYIKLFVLDE ADEMLSRGFK DQIQDVFKML
210 220 230 240 250
PPDVQVILLS ATMPPDVLEV SRCFMRDPVS ILVKKEELTL EGIKQFYVNV
260 270 280 290 300
KQENWKLGTL CDLYDTLSIT QSVIFCNTRR KVDQLTQEMS IHNFTVSAMH
310 320 330 340 350
GDMEQRDREV IMKQFRSGSS RVLITTDLLA RGIDVQQVSL VINYDLPSNR
360 370 380 390 400
ENYIHRIGRG GRFGRKGVAI NFITDDDRRI LKDIEQFYHT TIEEMPANIA

DLI
Length:403
Mass (Da):45,879
Last modified:December 1, 2000 - v3
Checksum:iB870FF8C420CC4F2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti167Missing in CAA48790 (PubMed:8455559).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69045 Genomic DNA Translation: CAA48790.1
AE014134 Genomic DNA Translation: AAF52317.2
AE014134 Genomic DNA Translation: AAN10566.1
AE014134 Genomic DNA Translation: AAN10567.1
AE014134 Genomic DNA Translation: AAN10568.1
AF145621 mRNA Translation: AAD38596.1
AY069283 mRNA Translation: AAL39428.1
AY121623 mRNA Translation: AAM51950.1
PIRiS30278
RefSeqiNP_001245907.1, NM_001258978.3
NP_001260117.1, NM_001273188.1
NP_476595.1, NM_057247.5
NP_723137.1, NM_164668.3
NP_723138.1, NM_164669.3
NP_723139.1, NM_164670.4
UniGeneiDm.7226

Genome annotation databases

EnsemblMetazoaiFBtr0079175; FBpp0078806; FBgn0001942
FBtr0079176; FBpp0078807; FBgn0001942
FBtr0079177; FBpp0078808; FBgn0001942
FBtr0079178; FBpp0078809; FBgn0001942
FBtr0307068; FBpp0297911; FBgn0001942
FBtr0331201; FBpp0303628; FBgn0001942
GeneIDi33835
KEGGidme:Dmel_CG9075

Similar proteinsi

Entry informationi

Entry nameiIF4A_DROME
AccessioniPrimary (citable) accession number: Q02748
Secondary accession number(s): A4UZV9, Q9U9Y6, Q9VMJ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: December 1, 2000
Last modified: May 23, 2018
This is version 165 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Translation initiation factors
    List of translation initiation factor entries
  3. SIMILARITY comments
    Index of protein domains and families
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