Q02734 (SIAT6_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase
EC=2.4.99.6

Alternative name(s):
Beta-galactoside alpha-2,3-sialyltransferase 3
Short name=Alpha 2,3-ST 3
Gal beta-1,3(4) GlcNAc alpha-2,3 sialyltransferase
N-acetyllactosaminide alpha-2,3-sialyltransferase
ST3Gal III
Short name=ST3GalIII
ST3N
Sialyltransferase 6

Cleaved into the following chain:

1. CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase soluble form

Gene names

Name:	St3gal3
Synonyms:	Siat6

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the formation of the NeuAc-alpha-2,3-Gal-beta-1,4-GlcNAc-, NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc- or NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- sequences found in terminal carbohydrate groups of glycoproteins and glycolipids. The highest activity is toward Gal-beta-1,3-GlcNAc and the lowest toward Gal-beta-1,3-GalNAc By similarity.
Catalytic activity	CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-glycoprotein = CMP + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-glycoprotein.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Secreted. Note: Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
Tissue specificity	Found in all tissues tested. High expression found in brain, liver, kidney, colon, heart and lung.
Post-translational modification	The soluble form derives from the membrane form by proteolytic processing.
Sequence similarities	Belongs to the glycosyltransferase 29 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane Secreted
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell integral to Golgi membrane Inferred from electronic annotation. Source: InterPro
Molecular function	N-acetyllactosaminide alpha-2,3-sialyltransferase activity Traceable author statement Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase		PRO_0000012143
	Chain	48 – 374	327	CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase soluble form		PRO_0000012144
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 28	20	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	29 – 374	346	Lumenal Potential
Sites
	Site	46 – 47	2	Cleavage; partial
Amino acid modifications
	Glycosylation	79	1	N-linked (GlcNAc...) Potential
	Glycosylation	170	1	N-linked (GlcNAc...) Potential
	Disulfide bond	159 ↔ 313		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q02734 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 16BAD1458963024C	FASTA	374	42,082
	10 20 30 40 50 60 MGLLVFVRNL LLALCLFLVL GFLYYSAWKL HLLQWEDSNS LILSLDSAGQ TLGTEYDRLG 70 80 90 100 110 120 FLLKLDSKLP AELATKYANF SEGACKPGYA SAMMTAIFPR FSKPAPMFLD DSFRKWARIR 130 140 150 160 170 180 EFVPPFGIKG QDNLIKAILS VTKEYRLTPA LDSLHCRRCI IVGNGGVLAN KSLGSRIDDY 190 200 210 220 230 240 DIVIRLNSAP VKGFEKDVGS KTTLRITYPE GAMQRPEQYE RDSLFVLAGF KWQDFKWLKY 250 260 270 280 290 300 IVYKERVSAS DGFWKSVATR VPKEPPEIRI LNPYFIQEAA FTLIGLPFNN GLMGRGNIPT 310 320 330 340 350 360 LGSVAVTMAL DGCDEVAVAG FGYDMNTPNA PLHYYETVRM AAIKESWTHN IQREKEFLRK 370 LVKARVITDL SSGI

References

[1]

"Primary structure of Gal beta 1,3(4)GlcNAc alpha 2,3-sialyltransferase determined by mass spectrometry sequence analysis and molecular cloning. Evidence for a protein motif in the sialyltransferase gene family." Wen D.X., Livingston B.D., Medzihradszky K.F., Kelm S., Burlingame A.L., Paulson J.C. J. Biol. Chem. 267:21011-21019(1992) [PubMed: 1400416] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M97754 mRNA. Translation: AAA42146.1.
IPI	IPI00210122.
PIR	A45074.
RefSeq	NP_113885.1. NM_031697.1.
UniGene	Rn.44390.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q02734.
Protein family/group databases
CAZy	GT29. Glycosyltransferase Family 29.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	64445.
KEGG	rno:64445.
Organism-specific databases
CTD	6487.
RGD	68414. St3gal3.
Phylogenomic databases
eggNOG	roNOG05023.
GeneTree	ENSGT00550000074199.
HOVERGEN	HBG056676.
InParanoid	Q02734.
Enzyme and pathway databases
BRENDA	2.4.99.6. 5301.
Gene expression databases
ArrayExpress	Q02734.
Genevestigator	Q02734.
GermOnline	ENSRNOG00000019843. Rattus norvegicus.
Family and domain databases
InterPro	IPR001675. Glyco_trans_29. IPR012163. Sialyl_trans. [Graphical view]
KO	K00781.
Pfam	PF00777. Glyco_transf_29. 1 hit. [Graphical view]
PIRSF	PIRSF005557. Sialyl_trans. 1 hit.
ProtoNet	Search...
Other
NextBio	613178.

Entry information

Entry name

SIAT6_RAT

Accession

Primary (citable) accession number: Q02734

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	November 16, 2011
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program