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Q02732 (CTF19_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Central kinetochore subunit CTF19
Alternative name(s):
Chromosome transmission fidelity protein 19
Minichromosome maintenance protein 18
Gene names
Name:CTF19
Synonyms:MCM18
Ordered Locus Names:YPL018W
ORF Names:LPB13W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length369 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation.

Subunit structure

Component of the heterotetrameric kinetochore subcomplex COMA, which consists of AME1, CTF19, MCM21 and OKP1. The COMA subcomplex is part of a larger central kinetochore CTF19 complex, which is composed of at least AME1, CHL4, CTF3, CTF19, IML3, MCM16, MCM21, MCM22, NKP1, NKP2 and OKP1. Interacts with the CTF3 complex subunits CTF3, MCM16 and MCM22 as well as CHL4 and IML3. Interacts with the N-terminal domain of CSE4 and with the CBF3 complex subunit A (CBF2). Ref.4 Ref.5 Ref.7 Ref.9

Subcellular location

Nucleus. Chromosomecentromerekinetochore. Note: Associated with kinetochores. Ref.1 Ref.4 Ref.5 Ref.7 Ref.9

Miscellaneous

Present with 1254 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the CTF19 family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 369369Central kinetochore subunit CTF19
PRO_0000079492

Regions

Coiled coil51 – 8737 Potential

Sequences

Sequence LengthMass (Da)Tools
Q02732 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 23B4CBD6AE26E793

FASTA36942,782
        10         20         30         40         50         60 
MDFTSDTTNS HDTSNSHLSL EDAVGTHHAG EADVNIDGDE KQQLSLLDDD QVRALKLQEE 

        70         80         90        100        110        120 
KDALLTRRNT LLQEIQTYQN ILMKENNSKT KNGDILQNDI TQDFLNLISI SSSNPNSAIS 

       130        140        150        160        170        180 
DRKRVERING LTNLQKELVT KYDTLPLLNM NLRLSYLRDH TYPHLQVSVQ SRDRVHNDGI 

       190        200        210        220        230        240 
EVLVVNYKFC RNTMNPFEIQ FKMFYKFEDS TLLKWEILRI STNVRLKAKQ LLATRNFQKC 

       250        260        270        280        290        300 
LLSLYEFDKI KSKKTGIFQN LINLLKRKTR CYLMNNSDSL IVERVIREGR LTTIKLQINF 

       310        320        330        340        350        360 
IITMPGERGK PRNCFLPMSK ISIALWKGGE RFNQIDLDEI CYGLIKEYGV KTGLKEICNV 


CLFPDMYAR 

« Hide

References

« Hide 'large scale' references
[1]"Ctf19p: a novel kinetochore protein in Saccharomyces cerevisiae and a potential link between the kinetochore and mitotic spindle."
Hyland K.M., Kingsbury J., Koshland D., Hieter P.
J. Cell Biol. 145:15-28(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"A putative protein complex consisting of Ctf19, Mcm21, and Okp1 represents a missing link in the budding yeast kinetochore."
Ortiz J., Stemmann O., Rank S., Lechner J.
Genes Dev. 13:1140-1155(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CBF2; MCM21 AND OKP1, SUBCELLULAR LOCATION.
[5]"The N-terminus of the centromere H3-like protein Cse4p performs an essential function distinct from that of the histone fold domain."
Chen Y., Baker R.E., Keith K.C., Harris K., Stoler S., Fitzgerald-Hayes M.
Mol. Cell. Biol. 20:7037-7048(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CSE4, SUBCELLULAR LOCATION.
[6]"Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p."
Cheeseman I.M., Anderson S., Jwa M., Green E.M., Kang J.-S., Yates J.R. III, Chan C.S.M., Drubin D.G., Barnes G.
Cell 111:163-172(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, COMPONENT OF CTF19 COMPLEX.
[7]"Ctf3p, the Mis6 budding yeast homolog, interacts with Mcm22p and Mcm16p at the yeast outer kinetochore."
Measday V., Hailey D.W., Pot I., Givan S.A., Hyland K.M., Cagney G., Fields S., Davis T.N., Hieter P.
Genes Dev. 16:101-113(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CTF3; MCM16 AND MCM22, SUBCELLULAR LOCATION.
[8]"Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes."
De Wulf P., McAinsh A.D., Sorger P.K.
Genes Dev. 17:2902-2921(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, COMPONENT OF COMA COMPLEX.
[9]"Chl4p and Iml3p are two new members of the budding yeast outer kinetochore."
Pot I., Measday V., Snydsman B., Cagney G., Fields S., Davis T.N., Muller E.G.D., Hieter P.
Mol. Biol. Cell 14:460-476(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CHL4; CTF3 AND IML3, SUBCELLULAR LOCATION.
[10]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U72265 Genomic DNA. Translation: AAB17275.1.
U36624 Genomic DNA. Translation: AAB68169.1.
BK006949 Genomic DNA. Translation: DAA11410.1.
PIRS63464.
RefSeqNP_015307.1. NM_001183832.1.

3D structure databases

ProteinModelPortalQ02732.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36159. 193 interactions.
DIPDIP-3009N.
IntActQ02732. 15 interactions.
MINTMINT-438402.
STRING4932.YPL018W.

Proteomic databases

PaxDbQ02732.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPL018W; YPL018W; YPL018W.
GeneID856089.
KEGGsce:YPL018W.

Organism-specific databases

CYGDYPL018w.
SGDS000005939. CTF19.

Phylogenomic databases

eggNOGNOG47118.
KOK11554.
OMAMRIESAN.
OrthoDBEOG7W9S4J.

Enzyme and pathway databases

BioCycYEAST:G3O-33937-MONOMER.

Gene expression databases

GenevestigatorQ02732.

Family and domain databases

ProtoNetSearch...

Other

NextBio981113.

Entry information

Entry nameCTF19_YEAST
AccessionPrimary (citable) accession number: Q02732
Secondary accession number(s): D6W3Z4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families