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Q02732

- CTF19_YEAST

UniProt

Q02732 - CTF19_YEAST

Protein

Central kinetochore subunit CTF19

Gene

CTF19

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation.

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. attachment of spindle microtubules to kinetochore Source: SGD
    2. chromosome segregation Source: SGD
    3. establishment of mitotic sister chromatid cohesion Source: SGD
    4. meiotic nuclear division Source: UniProtKB-KW
    5. spindle assembly checkpoint Source: SGD

    Keywords - Biological processi

    Cell cycle, Cell division, Meiosis, Mitosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33937-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Central kinetochore subunit CTF19
    Alternative name(s):
    Chromosome transmission fidelity protein 19
    Minichromosome maintenance protein 18
    Gene namesi
    Name:CTF19
    Synonyms:MCM18
    Ordered Locus Names:YPL018W
    ORF Names:LPB13W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPL018w.
    SGDiS000005939. CTF19.

    Subcellular locationi

    Nucleus. Chromosomecentromerekinetochore
    Note: Associated with kinetochores.

    GO - Cellular componenti

    1. COMA complex Source: SGD
    2. condensed nuclear chromosome kinetochore Source: SGD
    3. nucleus Source: SGD

    Keywords - Cellular componenti

    Centromere, Chromosome, Kinetochore, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 369369Central kinetochore subunit CTF19PRO_0000079492Add
    BLAST

    Proteomic databases

    MaxQBiQ02732.
    PaxDbiQ02732.

    Expressioni

    Gene expression databases

    GenevestigatoriQ02732.

    Interactioni

    Subunit structurei

    Component of the heterotetrameric kinetochore subcomplex COMA, which consists of AME1, CTF19, MCM21 and OKP1. The COMA subcomplex is part of a larger central kinetochore CTF19 complex, which is composed of at least AME1, CHL4, CTF3, CTF19, IML3, MCM16, MCM21, MCM22, NKP1, NKP2 and OKP1. Interacts with the CTF3 complex subunits CTF3, MCM16 and MCM22 as well as CHL4 and IML3. Interacts with the N-terminal domain of CSE4 and with the CBF3 complex subunit A (CBF2).4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CTF3Q127483EBI-5199,EBI-30457
    DAM1P532672EBI-5199,EBI-23268
    MCM16Q122622EBI-5199,EBI-31487

    Protein-protein interaction databases

    BioGridi36159. 194 interactions.
    DIPiDIP-3009N.
    IntActiQ02732. 15 interactions.
    MINTiMINT-438402.
    STRINGi4932.YPL018W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ02732.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili51 – 8737Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the CTF19 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG47118.
    KOiK11554.
    OMAiSEGINDA.
    OrthoDBiEOG7W9S4J.

    Sequencei

    Sequence statusi: Complete.

    Q02732-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDFTSDTTNS HDTSNSHLSL EDAVGTHHAG EADVNIDGDE KQQLSLLDDD    50
    QVRALKLQEE KDALLTRRNT LLQEIQTYQN ILMKENNSKT KNGDILQNDI 100
    TQDFLNLISI SSSNPNSAIS DRKRVERING LTNLQKELVT KYDTLPLLNM 150
    NLRLSYLRDH TYPHLQVSVQ SRDRVHNDGI EVLVVNYKFC RNTMNPFEIQ 200
    FKMFYKFEDS TLLKWEILRI STNVRLKAKQ LLATRNFQKC LLSLYEFDKI 250
    KSKKTGIFQN LINLLKRKTR CYLMNNSDSL IVERVIREGR LTTIKLQINF 300
    IITMPGERGK PRNCFLPMSK ISIALWKGGE RFNQIDLDEI CYGLIKEYGV 350
    KTGLKEICNV CLFPDMYAR 369
    Length:369
    Mass (Da):42,782
    Last modified:November 1, 1996 - v1
    Checksum:i23B4CBD6AE26E793
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U72265 Genomic DNA. Translation: AAB17275.1.
    U36624 Genomic DNA. Translation: AAB68169.1.
    BK006949 Genomic DNA. Translation: DAA11410.1.
    PIRiS63464.
    RefSeqiNP_015307.1. NM_001183832.1.

    Genome annotation databases

    EnsemblFungiiYPL018W; YPL018W; YPL018W.
    GeneIDi856089.
    KEGGisce:YPL018W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U72265 Genomic DNA. Translation: AAB17275.1 .
    U36624 Genomic DNA. Translation: AAB68169.1 .
    BK006949 Genomic DNA. Translation: DAA11410.1 .
    PIRi S63464.
    RefSeqi NP_015307.1. NM_001183832.1.

    3D structure databases

    ProteinModelPortali Q02732.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36159. 194 interactions.
    DIPi DIP-3009N.
    IntActi Q02732. 15 interactions.
    MINTi MINT-438402.
    STRINGi 4932.YPL018W.

    Proteomic databases

    MaxQBi Q02732.
    PaxDbi Q02732.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL018W ; YPL018W ; YPL018W .
    GeneIDi 856089.
    KEGGi sce:YPL018W.

    Organism-specific databases

    CYGDi YPL018w.
    SGDi S000005939. CTF19.

    Phylogenomic databases

    eggNOGi NOG47118.
    KOi K11554.
    OMAi SEGINDA.
    OrthoDBi EOG7W9S4J.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33937-MONOMER.

    Miscellaneous databases

    NextBioi 981113.

    Gene expression databases

    Genevestigatori Q02732.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Ctf19p: a novel kinetochore protein in Saccharomyces cerevisiae and a potential link between the kinetochore and mitotic spindle."
      Hyland K.M., Kingsbury J., Koshland D., Hieter P.
      J. Cell Biol. 145:15-28(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "A putative protein complex consisting of Ctf19, Mcm21, and Okp1 represents a missing link in the budding yeast kinetochore."
      Ortiz J., Stemmann O., Rank S., Lechner J.
      Genes Dev. 13:1140-1155(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CBF2; MCM21 AND OKP1, SUBCELLULAR LOCATION.
    5. "The N-terminus of the centromere H3-like protein Cse4p performs an essential function distinct from that of the histone fold domain."
      Chen Y., Baker R.E., Keith K.C., Harris K., Stoler S., Fitzgerald-Hayes M.
      Mol. Cell. Biol. 20:7037-7048(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CSE4, SUBCELLULAR LOCATION.
    6. "Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p."
      Cheeseman I.M., Anderson S., Jwa M., Green E.M., Kang J.-S., Yates J.R. III, Chan C.S.M., Drubin D.G., Barnes G.
      Cell 111:163-172(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, COMPONENT OF CTF19 COMPLEX.
    7. "Ctf3p, the Mis6 budding yeast homolog, interacts with Mcm22p and Mcm16p at the yeast outer kinetochore."
      Measday V., Hailey D.W., Pot I., Givan S.A., Hyland K.M., Cagney G., Fields S., Davis T.N., Hieter P.
      Genes Dev. 16:101-113(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CTF3; MCM16 AND MCM22, SUBCELLULAR LOCATION.
    8. "Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes."
      De Wulf P., McAinsh A.D., Sorger P.K.
      Genes Dev. 17:2902-2921(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, COMPONENT OF COMA COMPLEX.
    9. "Chl4p and Iml3p are two new members of the budding yeast outer kinetochore."
      Pot I., Measday V., Snydsman B., Cagney G., Fields S., Davis T.N., Muller E.G.D., Hieter P.
      Mol. Biol. Cell 14:460-476(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CHL4; CTF3 AND IML3, SUBCELLULAR LOCATION.
    10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCTF19_YEAST
    AccessioniPrimary (citable) accession number: Q02732
    Secondary accession number(s): D6W3Z4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1254 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3