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Protein

Vacuolar transporter chaperone 3

Gene

VTC3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the vacuolar transporter chaperone (VTC) complex, which plays a role in vacuolar membrane fusion. Required for LMA1 release prior to membrane fusion (PubMed:11823419). Binds inositol hexakisphosphate (Ins6P) and similar inositol polyphosphates, such as 5-diphospho-inositol pentakisphosphate (5-InsP7); these are important intracellular signaling molecules (PubMed:27080106). Inositol polyphosphate binding promotes vacuolar polyphosphate synthesis (PubMed:27080106).2 Publications

Miscellaneous

Present with 2630 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei22Important for inositol polyphosphate bindingBy similarity1
Sitei26Important for inositol polyphosphate bindingBy similarity1

GO - Molecular functioni

  • calmodulin binding Source: SGD
  • inositol hexakisphosphate binding Source: UniProtKB

GO - Biological processi

  • inositol phosphate-mediated signaling Source: UniProtKB
  • lysosomal microautophagy Source: SGD
  • polyphosphate biosynthetic process Source: UniProtKB
  • polyphosphate metabolic process Source: SGD
  • vacuolar transport Source: SGD
  • vacuole fusion, non-autophagic Source: SGD

Enzyme and pathway databases

BioCyciYEAST:YPL019C-MONOMER

Protein family/group databases

TCDBi4.E.1.1.2 the vacuolar (acidocalcisome) polyphosphate polymerase (v-ppp) family

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar transporter chaperone 3
Alternative name(s):
Phosphate metabolism protein 2
Gene namesi
Name:VTC3
Synonyms:PHM2
Ordered Locus Names:YPL019C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL019C
SGDiS000005940 VTC3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 709CytoplasmicAdd BLAST709
Transmembranei710 – 727HelicalSequence analysisAdd BLAST18
Topological domaini728 – 735Vacuolar8
Transmembranei736 – 756HelicalSequence analysisAdd BLAST21
Topological domaini757 – 778CytoplasmicAdd BLAST22
Transmembranei779 – 799HelicalSequence analysisAdd BLAST21
Topological domaini800 – 835VacuolarAdd BLAST36

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000659361 – 835Vacuolar transporter chaperone 3Add BLAST835

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei43PhosphoserineCombined sources1
Modified residuei45PhosphoserineCombined sources1
Modified residuei50PhosphoserineCombined sources1
Modified residuei183PhosphothreonineCombined sources1
Modified residuei195PhosphoserineCombined sources1
Modified residuei198PhosphoserineCombined sources1
Modified residuei270PhosphoserineCombined sources1
Modified residuei274PhosphoserineCombined sources1
Modified residuei589PhosphoserineCombined sources1
Modified residuei592PhosphoserineCombined sources1
Modified residuei621PhosphoserineCombined sources1
Modified residuei622PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ02725
PaxDbiQ02725
PRIDEiQ02725

PTM databases

CarbonylDBiQ02725
iPTMnetiQ02725

Expressioni

Inductioni

By low phosphate.1 Publication

Interactioni

Subunit structurei

The VTC complex is an integral membrane heterooligomer composed of VTC1, VTC2, VTC3 and VTC4. The complex interacts with the v-SNARE NYV1 and with the V0 subunit of V-ATPase VPH1.1 Publication

GO - Molecular functioni

  • calmodulin binding Source: SGD

Protein-protein interaction databases

BioGridi36158, 70 interactors
DIPiDIP-2000N
IntActiQ02725, 6 interactors
MINTiQ02725
STRINGi4932.YPL019C

Structurei

3D structure databases

ProteinModelPortaliQ02725
SMRiQ02725
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 145SPXPROSITE-ProRule annotationAdd BLAST145

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni126 – 133Important for inositol polyphosphate binding1 Publication8

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili385 – 413Sequence analysisAdd BLAST29

Domaini

The SPX domain has very high affinity for inositol polyphosphates, such as myo-inositol hexakisphosphate and 5-diphospho-myo-inositol pentakisphosphate (5-InsP7), and moderate affinity for inorganic pyrophosphate. Its affinity for inorganic phosphate is tow to three orders of magnitude lower.1 Publication

Sequence similaritiesi

Belongs to the VTC2/3 family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000014774
HOGENOMiHOG000209413
InParanoidiQ02725
OrthoDBiEOG092C0LBP

Family and domain databases

InterProiView protein in InterPro
IPR003807 DUF202
IPR004331 SPX_dom
IPR018966 VTC_domain
PfamiView protein in Pfam
PF02656 DUF202, 1 hit
PF09359 VTC, 1 hit
PROSITEiView protein in PROSITE
PS51382 SPX, 1 hit

Sequencei

Sequence statusi: Complete.

Q02725-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLFGIKLAND VYPPWKDSYI DYERLKKLLK ESVIHDGRSS VDSWSERNES
60 70 80 90 100
DFVEALDKEL EKVYTFQISK YNAVLRKLDD LEENTKSAEK IQKINSEQFK
110 120 130 140 150
NTLEECLDEA QRLDNFDRLN FTGFIKIVKK HDKLHPNYPS VKSLLQVRLK
160 170 180 190 200
ELPFNNSEEY SPLLYRISYL YEFLRSNYDH PNTVSKSLAS TSKLSHFSNL
210 220 230 240 250
EDASFKSYKF WVHDDNIMEV KARILRHLPA LVYASVPNEN DDFVDNLESD
260 270 280 290 300
VRVQPEARLN IGSKSNSLSS DGNSNQDVEI GKSKSVIFPQ SYDPTITTLY
310 320 330 340 350
FDNDFFDLYN NRLLKISGAP TLRLRWIGKL LDKPDIFLEK RTFTENTETG
360 370 380 390 400
NSSFEEIRLQ MKAKFINNFI FKNDPSYKNY LINQLRERGT QKEELEKLSR
410 420 430 440 450
DFDNIQNFIV EEKLQPVLRA TYNRTAFQIP GDQSIRVTID SNIMYIREDS
460 470 480 490 500
LDKNRPIRNP ENWHRDDIDS NIPNPLRFLR AGEYSKFPYS VMEIKVINQD
510 520 530 540 550
NSQMPNYEWI KDLTNSHLVN EVPKFSLYLQ GVASLFGEDD KYVNILPFWL
560 570 580 590 600
PDLETDIRKN PQEAYEEEKK TLQKQKSIHD KLDNMRRLSK ISVPDGKTTE
610 620 630 640 650
RQGQKDQNTR HVIADLEDHE SSDEEGTALP KKSAVKKGKK FKTNAAFLKI
660 670 680 690 700
LAGKNISENG NDPYSDDTDS ASSFQLPPGV KKPVHLLKNA GPVKVEAKVW
710 720 730 740 750
LANERTFNRW LSVTTLLSVL TFSIYNSVQK AEFPQLADLL AYVYFFLTLF
760 770 780 790 800
CGVWAYRTYL KRLTLIKGRS GKHLDAPVGP ILVAVVLIVT LVVNFSVAFK
810 820 830
EAARRERGLV NVSSQPSLPR TLKPIQDFIF NLVGE
Length:835
Mass (Da):96,553
Last modified:November 1, 1996 - v1
Checksum:iF098B36A9D9B9C11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36624 Genomic DNA Translation: AAB68168.1
BK006949 Genomic DNA Translation: DAA11409.1
PIRiS63463
RefSeqiNP_015306.1, NM_001183833.1

Genome annotation databases

EnsemblFungiiYPL019C; YPL019C; YPL019C
GeneIDi856088
KEGGisce:YPL019C

Similar proteinsi

Entry informationi

Entry nameiVTC3_YEAST
AccessioniPrimary (citable) accession number: Q02725
Secondary accession number(s): D6W3Z3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

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