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Protein

Vacuolar transporter chaperone 3

Gene

VTC3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the vacuolar transporter chaperone (VTC) complex, which plays a role in vacuolar membrane fusion. Required for LMA1 release prior to membrane fusion (PubMed:11823419). Binds inositol hexakisphosphate (Ins6P) and similar inositol polyphosphates, such as 5-diphospho-inositol pentakisphosphate (5-InsP7); these are important intracellular signaling molecules (PubMed:27080106). Inositol polyphosphate binding promotes vacuolar polyphosphate synthesis (PubMed:27080106).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei22Important for inositol polyphosphate bindingBy similarity1
Sitei26Important for inositol polyphosphate bindingBy similarity1

GO - Molecular functioni

  • inositol hexakisphosphate binding Source: UniProtKB

GO - Biological processi

  • inositol phosphate-mediated signaling Source: UniProtKB
  • lysosomal microautophagy Source: SGD
  • polyphosphate biosynthetic process Source: UniProtKB
  • polyphosphate metabolic process Source: SGD
  • vacuolar transport Source: SGD
  • vacuole fusion, non-autophagic Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:YPL019C-MONOMER.

Protein family/group databases

TCDBi4.E.1.1.2. the vacuolar (acidocalcisome) polyphosphate polymerase (v-ppp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar transporter chaperone 3
Alternative name(s):
Phosphate metabolism protein 2
Gene namesi
Name:VTC3
Synonyms:PHM2
Ordered Locus Names:YPL019C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL019C.
SGDiS000005940. VTC3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 709CytoplasmicAdd BLAST709
Transmembranei710 – 727HelicalSequence analysisAdd BLAST18
Topological domaini728 – 735Vacuolar8
Transmembranei736 – 756HelicalSequence analysisAdd BLAST21
Topological domaini757 – 778CytoplasmicAdd BLAST22
Transmembranei779 – 799HelicalSequence analysisAdd BLAST21
Topological domaini800 – 835VacuolarAdd BLAST36

GO - Cellular componenti

  • endoplasmic reticulum Source: SGD
  • fungal-type vacuole membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • vacuolar transporter chaperone complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000659361 – 835Vacuolar transporter chaperone 3Add BLAST835

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei43PhosphoserineCombined sources1
Modified residuei45PhosphoserineCombined sources1
Modified residuei50PhosphoserineCombined sources1
Modified residuei183PhosphothreonineCombined sources1
Modified residuei195PhosphoserineCombined sources1
Modified residuei198PhosphoserineCombined sources1
Modified residuei270PhosphoserineCombined sources1
Modified residuei274PhosphoserineCombined sources1
Modified residuei589PhosphoserineCombined sources1
Modified residuei592PhosphoserineCombined sources1
Modified residuei621PhosphoserineCombined sources1
Modified residuei622PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ02725.
PRIDEiQ02725.

PTM databases

iPTMnetiQ02725.

Expressioni

Inductioni

By low phosphate.1 Publication

Interactioni

Subunit structurei

The VTC complex is an integral membrane heterooligomer composed of VTC1, VTC2, VTC3 and VTC4. The complex interacts with the v-SNARE NYV1 and with the V0 subunit of V-ATPase VPH1.1 Publication

Protein-protein interaction databases

BioGridi36158. 27 interactors.
DIPiDIP-2000N.
IntActiQ02725. 6 interactors.
MINTiMINT-393829.

Structurei

3D structure databases

ProteinModelPortaliQ02725.
SMRiQ02725.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 145SPXPROSITE-ProRule annotationAdd BLAST145

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni126 – 133Important for inositol polyphosphate binding1 Publication8

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili385 – 413Sequence analysisAdd BLAST29

Domaini

The SPX domain has very high affinity for inositol polyphosphates, such as myo-inositol hexakisphosphate and 5-diphospho-myo-inositol pentakisphosphate (5-InsP7), and moderate affinity for inorganic pyrophosphate. Its affinity for inorganic phosphate is tow to three orders of magnitude lower.1 Publication

Sequence similaritiesi

Belongs to the VTC2/3 family.Curated
Contains 1 SPX domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000014774.
HOGENOMiHOG000209413.
InParanoidiQ02725.
OMAiVLEIREC.
OrthoDBiEOG092C0LBP.

Family and domain databases

InterProiIPR003807. DUF202.
IPR004331. SPX_dom.
IPR018966. VTC_domain.
[Graphical view]
PfamiPF02656. DUF202. 1 hit.
PF09359. VTC. 1 hit.
[Graphical view]
PROSITEiPS51382. SPX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q02725-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLFGIKLAND VYPPWKDSYI DYERLKKLLK ESVIHDGRSS VDSWSERNES
60 70 80 90 100
DFVEALDKEL EKVYTFQISK YNAVLRKLDD LEENTKSAEK IQKINSEQFK
110 120 130 140 150
NTLEECLDEA QRLDNFDRLN FTGFIKIVKK HDKLHPNYPS VKSLLQVRLK
160 170 180 190 200
ELPFNNSEEY SPLLYRISYL YEFLRSNYDH PNTVSKSLAS TSKLSHFSNL
210 220 230 240 250
EDASFKSYKF WVHDDNIMEV KARILRHLPA LVYASVPNEN DDFVDNLESD
260 270 280 290 300
VRVQPEARLN IGSKSNSLSS DGNSNQDVEI GKSKSVIFPQ SYDPTITTLY
310 320 330 340 350
FDNDFFDLYN NRLLKISGAP TLRLRWIGKL LDKPDIFLEK RTFTENTETG
360 370 380 390 400
NSSFEEIRLQ MKAKFINNFI FKNDPSYKNY LINQLRERGT QKEELEKLSR
410 420 430 440 450
DFDNIQNFIV EEKLQPVLRA TYNRTAFQIP GDQSIRVTID SNIMYIREDS
460 470 480 490 500
LDKNRPIRNP ENWHRDDIDS NIPNPLRFLR AGEYSKFPYS VMEIKVINQD
510 520 530 540 550
NSQMPNYEWI KDLTNSHLVN EVPKFSLYLQ GVASLFGEDD KYVNILPFWL
560 570 580 590 600
PDLETDIRKN PQEAYEEEKK TLQKQKSIHD KLDNMRRLSK ISVPDGKTTE
610 620 630 640 650
RQGQKDQNTR HVIADLEDHE SSDEEGTALP KKSAVKKGKK FKTNAAFLKI
660 670 680 690 700
LAGKNISENG NDPYSDDTDS ASSFQLPPGV KKPVHLLKNA GPVKVEAKVW
710 720 730 740 750
LANERTFNRW LSVTTLLSVL TFSIYNSVQK AEFPQLADLL AYVYFFLTLF
760 770 780 790 800
CGVWAYRTYL KRLTLIKGRS GKHLDAPVGP ILVAVVLIVT LVVNFSVAFK
810 820 830
EAARRERGLV NVSSQPSLPR TLKPIQDFIF NLVGE
Length:835
Mass (Da):96,553
Last modified:November 1, 1996 - v1
Checksum:iF098B36A9D9B9C11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36624 Genomic DNA. Translation: AAB68168.1.
BK006949 Genomic DNA. Translation: DAA11409.1.
PIRiS63463.
RefSeqiNP_015306.1. NM_001183833.1.

Genome annotation databases

EnsemblFungiiYPL019C; YPL019C; YPL019C.
GeneIDi856088.
KEGGisce:YPL019C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36624 Genomic DNA. Translation: AAB68168.1.
BK006949 Genomic DNA. Translation: DAA11409.1.
PIRiS63463.
RefSeqiNP_015306.1. NM_001183833.1.

3D structure databases

ProteinModelPortaliQ02725.
SMRiQ02725.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36158. 27 interactors.
DIPiDIP-2000N.
IntActiQ02725. 6 interactors.
MINTiMINT-393829.

Protein family/group databases

TCDBi4.E.1.1.2. the vacuolar (acidocalcisome) polyphosphate polymerase (v-ppp) family.

PTM databases

iPTMnetiQ02725.

Proteomic databases

MaxQBiQ02725.
PRIDEiQ02725.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL019C; YPL019C; YPL019C.
GeneIDi856088.
KEGGisce:YPL019C.

Organism-specific databases

EuPathDBiFungiDB:YPL019C.
SGDiS000005940. VTC3.

Phylogenomic databases

GeneTreeiENSGT00390000014774.
HOGENOMiHOG000209413.
InParanoidiQ02725.
OMAiVLEIREC.
OrthoDBiEOG092C0LBP.

Enzyme and pathway databases

BioCyciYEAST:YPL019C-MONOMER.

Miscellaneous databases

PROiQ02725.

Family and domain databases

InterProiIPR003807. DUF202.
IPR004331. SPX_dom.
IPR018966. VTC_domain.
[Graphical view]
PfamiPF02656. DUF202. 1 hit.
PF09359. VTC. 1 hit.
[Graphical view]
PROSITEiPS51382. SPX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVTC3_YEAST
AccessioniPrimary (citable) accession number: Q02725
Secondary accession number(s): D6W3Z3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2630 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.