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Protein

Ubiquitin-like-specific protease 1

Gene

ULP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SMT3 to its mature form and deconjugation of SMT3 from targeted proteins. Has an essential role in the G2/M phase of the cell cycle.1 Publication

Catalytic activityi

Hydrolysis of the alpha-linked peptide bond in the sequence Gly-Gly-|-Ala-Thr-Tyr at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptide, Smt3, leading to the mature form of the protein. A second reaction involves the cleavage of an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei514 – 5141
Active sitei531 – 5311
Active sitei580 – 5801

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: SGD
  2. SUMO-specific protease activity Source: SGD

GO - Biological processi

  1. G2/M transition of mitotic cell cycle Source: SGD
  2. protein desumoylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-33938-MONOMER.
BRENDAi3.4.22.68. 984.
ReactomeiREACT_262998. SUMO is proteolytically processed.

Protein family/group databases

MEROPSiC48.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-like-specific protease 1 (EC:3.4.22.68)
Gene namesi
Name:ULP1
Ordered Locus Names:YPL020C
ORF Names:LPB11C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPL020c.
SGDiS000005941. ULP1.

Subcellular locationi

GO - Cellular componenti

  1. nuclear envelope Source: SGD
  2. nucleolus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 621620Ubiquitin-like-specific protease 1PRO_0000101731Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei21 – 211Phosphoserine1 Publication
Modified residuei25 – 251Phosphoserine1 Publication
Modified residuei179 – 1791Phosphothreonine2 Publications
Modified residuei264 – 2641Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ02724.
PaxDbiQ02724.

Expressioni

Gene expression databases

GenevestigatoriQ02724.

Interactioni

Protein-protein interaction databases

BioGridi36157. 255 interactions.
DIPiDIP-4041N.
IntActiQ02724. 13 interactions.
MINTiMINT-421115.
STRINGi4932.YPL020C.

Structurei

Secondary structure

1
621
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi409 – 41911Combined sources
Beta strandi426 – 4305Combined sources
Beta strandi433 – 4364Combined sources
Helixi437 – 4404Combined sources
Helixi441 – 4433Combined sources
Helixi451 – 46414Combined sources
Beta strandi468 – 4703Combined sources
Helixi474 – 4829Combined sources
Helixi484 – 4863Combined sources
Turni487 – 4915Combined sources
Helixi492 – 4943Combined sources
Helixi498 – 5003Combined sources
Beta strandi502 – 5109Combined sources
Turni511 – 5133Combined sources
Beta strandi514 – 5218Combined sources
Turni522 – 5254Combined sources
Beta strandi526 – 5305Combined sources
Helixi539 – 55517Combined sources
Beta strandi558 – 5603Combined sources
Beta strandi565 – 5695Combined sources
Beta strandi575 – 5784Combined sources
Helixi580 – 59213Combined sources
Helixi601 – 61616Combined sources
Turni617 – 6204Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EUVX-ray1.60A403-621[»]
2HKPX-ray2.10A403-621[»]
2HL8X-ray2.00A403-621[»]
2HL9X-ray1.90A403-621[»]
ProteinModelPortaliQ02724.
SMRiQ02724. Positions 403-621.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ02724.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni432 – 621190ProteaseAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C48 family.Curated

Phylogenomic databases

eggNOGiCOG5160.
GeneTreeiENSGT00530000062941.
HOGENOMiHOG000141768.
InParanoidiQ02724.
KOiK08592.
OMAiPRRWLND.
OrthoDBiEOG7ZKSMM.

Family and domain databases

InterProiIPR003653. Peptidase_C48.
[Graphical view]
PfamiPF02902. Peptidase_C48. 1 hit.
[Graphical view]
PROSITEiPS50600. ULP_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02724-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVEVDKHRN TLQYHKKNPY SPLFSPISTY RCYPRVLNNP SESRRSASFS
60 70 80 90 100
GIYKKRTNTS RFNYLNDRRV LSMEESMKDG SDRASKAGFI GGIRETLWNS
110 120 130 140 150
GKYLWHTFVK NEPRNFDGSE VEASGNSDVE SRSSGSRSSD VPYGLRENYS
160 170 180 190 200
SDTRKHKFDT STWALPNKRR RIESEGVGTP STSPISSLAS QKSNCDSDNS
210 220 230 240 250
ITFSRDPFGW NKWKTSAIGS NSENNTSDQK NSYDRRQYGT AFIRKKKVAK
260 270 280 290 300
QNINNTKLVS RAQSEEVTYL RQIFNGEYKV PKILKEERER QLKLMDMDKE
310 320 330 340 350
KDTGLKKSII DLTEKIKTIL IENNKNRLQT RNENDDDLVF VKEKKISSLE
360 370 380 390 400
RKHKDYLNQK LKFDRSILEF EKDFKRYNEI LNERKKIQED LKKKKEQLAK
410 420 430 440 450
KKLVPELNEK DDDQVQKALA SRENTQLMNR DNIEITVRDF KTLAPRRWLN
460 470 480 490 500
DTIIEFFMKY IEKSTPNTVA FNSFFYTNLS ERGYQGVRRW MKRKKTQIDK
510 520 530 540 550
LDKIFTPINL NQSHWALGII DLKKKTIGYV DSLSNGPNAM SFAILTDLQK
560 570 580 590 600
YVMEESKHTI GEDFDLIHLD CPQQPNGYDC GIYVCMNTLY GSADAPLDFD
610 620
YKDAIRMRRF IAHLILTDAL K
Length:621
Mass (Da):72,378
Last modified:November 1, 1996 - v1
Checksum:iF71132817FAF0B41
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36624 Genomic DNA. Translation: AAB68167.1.
BK006949 Genomic DNA. Translation: DAA11408.1.
PIRiS63462.
RefSeqiNP_015305.1. NM_001183834.1.

Genome annotation databases

EnsemblFungiiYPL020C; YPL020C; YPL020C.
GeneIDi856087.
KEGGisce:YPL020C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36624 Genomic DNA. Translation: AAB68167.1.
BK006949 Genomic DNA. Translation: DAA11408.1.
PIRiS63462.
RefSeqiNP_015305.1. NM_001183834.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EUVX-ray1.60A403-621[»]
2HKPX-ray2.10A403-621[»]
2HL8X-ray2.00A403-621[»]
2HL9X-ray1.90A403-621[»]
ProteinModelPortaliQ02724.
SMRiQ02724. Positions 403-621.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36157. 255 interactions.
DIPiDIP-4041N.
IntActiQ02724. 13 interactions.
MINTiMINT-421115.
STRINGi4932.YPL020C.

Protein family/group databases

MEROPSiC48.001.

Proteomic databases

MaxQBiQ02724.
PaxDbiQ02724.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL020C; YPL020C; YPL020C.
GeneIDi856087.
KEGGisce:YPL020C.

Organism-specific databases

CYGDiYPL020c.
SGDiS000005941. ULP1.

Phylogenomic databases

eggNOGiCOG5160.
GeneTreeiENSGT00530000062941.
HOGENOMiHOG000141768.
InParanoidiQ02724.
KOiK08592.
OMAiPRRWLND.
OrthoDBiEOG7ZKSMM.

Enzyme and pathway databases

BioCyciYEAST:G3O-33938-MONOMER.
BRENDAi3.4.22.68. 984.
ReactomeiREACT_262998. SUMO is proteolytically processed.

Miscellaneous databases

EvolutionaryTraceiQ02724.
NextBioi981107.

Gene expression databases

GenevestigatoriQ02724.

Family and domain databases

InterProiIPR003653. Peptidase_C48.
[Graphical view]
PfamiPF02902. Peptidase_C48. 1 hit.
[Graphical view]
PROSITEiPS50600. ULP_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "A new protease required for cell-cycle progression in yeast."
    Li S.J., Hochstrasser M.
    Nature 398:246-251(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21; SER-25 AND THR-179, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-179 AND SER-264, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  8. "Ulp1-SUMO crystal structure and genetic analysis reveal conserved interactions and a regulatory element essential for cell growth in yeast."
    Mossessova E., Lima C.D.
    Mol. Cell 5:865-876(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 401-621 IN COMPLEX WITH SMT3.

Entry informationi

Entry nameiULP1_YEAST
AccessioniPrimary (citable) accession number: Q02724
Secondary accession number(s): D6W3Z2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 377 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.