Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q02724

- ULP1_YEAST

UniProt

Q02724 - ULP1_YEAST

Protein

Ubiquitin-like-specific protease 1

Gene

ULP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SMT3 to its mature form and deconjugation of SMT3 from targeted proteins. Has an essential role in the G2/M phase of the cell cycle.1 Publication

    Catalytic activityi

    Hydrolysis of the alpha-linked peptide bond in the sequence Gly-Gly-|-Ala-Thr-Tyr at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptide, Smt3, leading to the mature form of the protein. A second reaction involves the cleavage of an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei514 – 5141
    Active sitei531 – 5311
    Active sitei580 – 5801

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: SGD
    2. SUMO-specific protease activity Source: SGD

    GO - Biological processi

    1. G2/M transition of mitotic cell cycle Source: SGD
    2. protein desumoylation Source: SGD

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33938-MONOMER.
    BRENDAi3.4.22.68. 984.

    Protein family/group databases

    MEROPSiC48.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-like-specific protease 1 (EC:3.4.22.68)
    Gene namesi
    Name:ULP1
    Ordered Locus Names:YPL020C
    ORF Names:LPB11C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPL020c.
    SGDiS000005941. ULP1.

    Subcellular locationi

    GO - Cellular componenti

    1. nuclear envelope Source: SGD
    2. nucleolus Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 621620Ubiquitin-like-specific protease 1PRO_0000101731Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei21 – 211Phosphoserine1 Publication
    Modified residuei25 – 251Phosphoserine1 Publication
    Modified residuei179 – 1791Phosphothreonine2 Publications
    Modified residuei264 – 2641Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ02724.
    PaxDbiQ02724.

    Expressioni

    Gene expression databases

    GenevestigatoriQ02724.

    Interactioni

    Protein-protein interaction databases

    BioGridi36157. 255 interactions.
    DIPiDIP-4041N.
    IntActiQ02724. 13 interactions.
    MINTiMINT-421115.
    STRINGi4932.YPL020C.

    Structurei

    Secondary structure

    1
    621
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi409 – 41911
    Beta strandi426 – 4305
    Beta strandi433 – 4364
    Helixi437 – 4404
    Helixi441 – 4433
    Helixi451 – 46414
    Beta strandi468 – 4703
    Helixi474 – 4829
    Helixi484 – 4863
    Turni487 – 4915
    Helixi492 – 4943
    Helixi498 – 5003
    Beta strandi502 – 5109
    Turni511 – 5133
    Beta strandi514 – 5218
    Turni522 – 5254
    Beta strandi526 – 5305
    Helixi539 – 55517
    Beta strandi558 – 5603
    Beta strandi565 – 5695
    Beta strandi575 – 5784
    Helixi580 – 59213
    Helixi601 – 61616
    Turni617 – 6204

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1EUVX-ray1.60A403-621[»]
    2HKPX-ray2.10A403-621[»]
    2HL8X-ray2.00A403-621[»]
    2HL9X-ray1.90A403-621[»]
    ProteinModelPortaliQ02724.
    SMRiQ02724. Positions 403-621.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ02724.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni432 – 621190ProteaseAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C48 family.Curated

    Phylogenomic databases

    eggNOGiCOG5160.
    GeneTreeiENSGT00530000062941.
    HOGENOMiHOG000141768.
    KOiK08592.
    OMAiPRRWLND.
    OrthoDBiEOG7ZKSMM.

    Family and domain databases

    InterProiIPR003653. Peptidase_C48.
    [Graphical view]
    PfamiPF02902. Peptidase_C48. 1 hit.
    [Graphical view]
    PROSITEiPS50600. ULP_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q02724-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSVEVDKHRN TLQYHKKNPY SPLFSPISTY RCYPRVLNNP SESRRSASFS    50
    GIYKKRTNTS RFNYLNDRRV LSMEESMKDG SDRASKAGFI GGIRETLWNS 100
    GKYLWHTFVK NEPRNFDGSE VEASGNSDVE SRSSGSRSSD VPYGLRENYS 150
    SDTRKHKFDT STWALPNKRR RIESEGVGTP STSPISSLAS QKSNCDSDNS 200
    ITFSRDPFGW NKWKTSAIGS NSENNTSDQK NSYDRRQYGT AFIRKKKVAK 250
    QNINNTKLVS RAQSEEVTYL RQIFNGEYKV PKILKEERER QLKLMDMDKE 300
    KDTGLKKSII DLTEKIKTIL IENNKNRLQT RNENDDDLVF VKEKKISSLE 350
    RKHKDYLNQK LKFDRSILEF EKDFKRYNEI LNERKKIQED LKKKKEQLAK 400
    KKLVPELNEK DDDQVQKALA SRENTQLMNR DNIEITVRDF KTLAPRRWLN 450
    DTIIEFFMKY IEKSTPNTVA FNSFFYTNLS ERGYQGVRRW MKRKKTQIDK 500
    LDKIFTPINL NQSHWALGII DLKKKTIGYV DSLSNGPNAM SFAILTDLQK 550
    YVMEESKHTI GEDFDLIHLD CPQQPNGYDC GIYVCMNTLY GSADAPLDFD 600
    YKDAIRMRRF IAHLILTDAL K 621
    Length:621
    Mass (Da):72,378
    Last modified:November 1, 1996 - v1
    Checksum:iF71132817FAF0B41
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U36624 Genomic DNA. Translation: AAB68167.1.
    BK006949 Genomic DNA. Translation: DAA11408.1.
    PIRiS63462.
    RefSeqiNP_015305.1. NM_001183834.1.

    Genome annotation databases

    EnsemblFungiiYPL020C; YPL020C; YPL020C.
    GeneIDi856087.
    KEGGisce:YPL020C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U36624 Genomic DNA. Translation: AAB68167.1 .
    BK006949 Genomic DNA. Translation: DAA11408.1 .
    PIRi S63462.
    RefSeqi NP_015305.1. NM_001183834.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1EUV X-ray 1.60 A 403-621 [» ]
    2HKP X-ray 2.10 A 403-621 [» ]
    2HL8 X-ray 2.00 A 403-621 [» ]
    2HL9 X-ray 1.90 A 403-621 [» ]
    ProteinModelPortali Q02724.
    SMRi Q02724. Positions 403-621.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36157. 255 interactions.
    DIPi DIP-4041N.
    IntActi Q02724. 13 interactions.
    MINTi MINT-421115.
    STRINGi 4932.YPL020C.

    Protein family/group databases

    MEROPSi C48.001.

    Proteomic databases

    MaxQBi Q02724.
    PaxDbi Q02724.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL020C ; YPL020C ; YPL020C .
    GeneIDi 856087.
    KEGGi sce:YPL020C.

    Organism-specific databases

    CYGDi YPL020c.
    SGDi S000005941. ULP1.

    Phylogenomic databases

    eggNOGi COG5160.
    GeneTreei ENSGT00530000062941.
    HOGENOMi HOG000141768.
    KOi K08592.
    OMAi PRRWLND.
    OrthoDBi EOG7ZKSMM.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33938-MONOMER.
    BRENDAi 3.4.22.68. 984.

    Miscellaneous databases

    EvolutionaryTracei Q02724.
    NextBioi 981107.

    Gene expression databases

    Genevestigatori Q02724.

    Family and domain databases

    InterProi IPR003653. Peptidase_C48.
    [Graphical view ]
    Pfami PF02902. Peptidase_C48. 1 hit.
    [Graphical view ]
    PROSITEi PS50600. ULP_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "A new protease required for cell-cycle progression in yeast."
      Li S.J., Hochstrasser M.
      Nature 398:246-251(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21; SER-25 AND THR-179, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-179 AND SER-264, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    8. "Ulp1-SUMO crystal structure and genetic analysis reveal conserved interactions and a regulatory element essential for cell growth in yeast."
      Mossessova E., Lima C.D.
      Mol. Cell 5:865-876(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 401-621 IN COMPLEX WITH SMT3.

    Entry informationi

    Entry nameiULP1_YEAST
    AccessioniPrimary (citable) accession number: Q02724
    Secondary accession number(s): D6W3Z2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 18, 2001
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 129 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 377 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3