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Protein

Ubiquitin-like-specific protease 1

Gene

ULP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SMT3 to its mature form and deconjugation of SMT3 from targeted proteins. Has an essential role in the G2/M phase of the cell cycle.1 Publication

Catalytic activityi

Hydrolysis of the alpha-linked peptide bond in the sequence Gly-Gly-|-Ala-Thr-Tyr at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptide, Smt3, leading to the mature form of the protein. A second reaction involves the cleavage of an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei514 – 51411 Publication
Active sitei531 – 53111 Publication
Active sitei580 – 58011 Publication

GO - Molecular functioni

  • cysteine-type peptidase activity Source: SGD
  • endopeptidase activity Source: GO_Central
  • SUMO-specific protease activity Source: SGD

GO - Biological processi

  • G2/M transition of mitotic cell cycle Source: SGD
  • protein desumoylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-33938-MONOMER.
BRENDAi3.4.22.68. 984.
ReactomeiR-SCE-3065679. SUMO is proteolytically processed.

Protein family/group databases

MEROPSiC48.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-like-specific protease 1 (EC:3.4.22.681 Publication)
Gene namesi
Name:ULP1
Ordered Locus Names:YPL020C
ORF Names:LPB11C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL020C.
SGDiS000005941. ULP1.

Subcellular locationi

GO - Cellular componenti

  • nuclear envelope Source: SGD
  • nucleolus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 621620Ubiquitin-like-specific protease 1PRO_0000101731Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources
Modified residuei21 – 211PhosphoserineCombined sources
Modified residuei25 – 251PhosphoserineCombined sources
Modified residuei179 – 1791PhosphothreonineCombined sources
Modified residuei264 – 2641PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ02724.
TopDownProteomicsiQ02724.

PTM databases

iPTMnetiQ02724.

Interactioni

Protein-protein interaction databases

BioGridi36157. 257 interactions.
DIPiDIP-4041N.
IntActiQ02724. 13 interactions.
MINTiMINT-421115.

Structurei

Secondary structure

1
621
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi409 – 41911Combined sources
Beta strandi426 – 4305Combined sources
Beta strandi433 – 4364Combined sources
Helixi437 – 4404Combined sources
Helixi441 – 4433Combined sources
Helixi451 – 46414Combined sources
Beta strandi468 – 4703Combined sources
Helixi474 – 4829Combined sources
Helixi484 – 4863Combined sources
Turni487 – 4915Combined sources
Helixi492 – 4943Combined sources
Helixi498 – 5003Combined sources
Beta strandi502 – 5109Combined sources
Turni511 – 5133Combined sources
Beta strandi514 – 5218Combined sources
Turni522 – 5254Combined sources
Beta strandi526 – 5305Combined sources
Helixi539 – 55517Combined sources
Beta strandi558 – 5603Combined sources
Beta strandi565 – 5695Combined sources
Beta strandi575 – 5784Combined sources
Helixi580 – 59213Combined sources
Helixi601 – 61616Combined sources
Turni617 – 6204Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EUVX-ray1.60A403-621[»]
2HKPX-ray2.10A403-621[»]
2HL8X-ray2.00A403-621[»]
2HL9X-ray1.90A403-621[»]
ProteinModelPortaliQ02724.
SMRiQ02724. Positions 403-621.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ02724.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni432 – 621190Protease1 PublicationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C48 family.Curated

Phylogenomic databases

GeneTreeiENSGT00530000062941.
HOGENOMiHOG000141768.
InParanoidiQ02724.
KOiK08592.
OMAiPRRWLND.
OrthoDBiEOG092C2417.

Family and domain databases

InterProiIPR003653. Peptidase_C48_C.
[Graphical view]
PfamiPF02902. Peptidase_C48. 1 hit.
[Graphical view]
PROSITEiPS50600. ULP_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02724-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVEVDKHRN TLQYHKKNPY SPLFSPISTY RCYPRVLNNP SESRRSASFS
60 70 80 90 100
GIYKKRTNTS RFNYLNDRRV LSMEESMKDG SDRASKAGFI GGIRETLWNS
110 120 130 140 150
GKYLWHTFVK NEPRNFDGSE VEASGNSDVE SRSSGSRSSD VPYGLRENYS
160 170 180 190 200
SDTRKHKFDT STWALPNKRR RIESEGVGTP STSPISSLAS QKSNCDSDNS
210 220 230 240 250
ITFSRDPFGW NKWKTSAIGS NSENNTSDQK NSYDRRQYGT AFIRKKKVAK
260 270 280 290 300
QNINNTKLVS RAQSEEVTYL RQIFNGEYKV PKILKEERER QLKLMDMDKE
310 320 330 340 350
KDTGLKKSII DLTEKIKTIL IENNKNRLQT RNENDDDLVF VKEKKISSLE
360 370 380 390 400
RKHKDYLNQK LKFDRSILEF EKDFKRYNEI LNERKKIQED LKKKKEQLAK
410 420 430 440 450
KKLVPELNEK DDDQVQKALA SRENTQLMNR DNIEITVRDF KTLAPRRWLN
460 470 480 490 500
DTIIEFFMKY IEKSTPNTVA FNSFFYTNLS ERGYQGVRRW MKRKKTQIDK
510 520 530 540 550
LDKIFTPINL NQSHWALGII DLKKKTIGYV DSLSNGPNAM SFAILTDLQK
560 570 580 590 600
YVMEESKHTI GEDFDLIHLD CPQQPNGYDC GIYVCMNTLY GSADAPLDFD
610 620
YKDAIRMRRF IAHLILTDAL K
Length:621
Mass (Da):72,378
Last modified:November 1, 1996 - v1
Checksum:iF71132817FAF0B41
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36624 Genomic DNA. Translation: AAB68167.1.
BK006949 Genomic DNA. Translation: DAA11408.1.
PIRiS63462.
RefSeqiNP_015305.1. NM_001183834.1.

Genome annotation databases

EnsemblFungiiYPL020C; YPL020C; YPL020C.
GeneIDi856087.
KEGGisce:YPL020C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36624 Genomic DNA. Translation: AAB68167.1.
BK006949 Genomic DNA. Translation: DAA11408.1.
PIRiS63462.
RefSeqiNP_015305.1. NM_001183834.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EUVX-ray1.60A403-621[»]
2HKPX-ray2.10A403-621[»]
2HL8X-ray2.00A403-621[»]
2HL9X-ray1.90A403-621[»]
ProteinModelPortaliQ02724.
SMRiQ02724. Positions 403-621.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36157. 257 interactions.
DIPiDIP-4041N.
IntActiQ02724. 13 interactions.
MINTiMINT-421115.

Protein family/group databases

MEROPSiC48.001.

PTM databases

iPTMnetiQ02724.

Proteomic databases

MaxQBiQ02724.
TopDownProteomicsiQ02724.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL020C; YPL020C; YPL020C.
GeneIDi856087.
KEGGisce:YPL020C.

Organism-specific databases

EuPathDBiFungiDB:YPL020C.
SGDiS000005941. ULP1.

Phylogenomic databases

GeneTreeiENSGT00530000062941.
HOGENOMiHOG000141768.
InParanoidiQ02724.
KOiK08592.
OMAiPRRWLND.
OrthoDBiEOG092C2417.

Enzyme and pathway databases

BioCyciYEAST:G3O-33938-MONOMER.
BRENDAi3.4.22.68. 984.
ReactomeiR-SCE-3065679. SUMO is proteolytically processed.

Miscellaneous databases

EvolutionaryTraceiQ02724.
PROiQ02724.

Family and domain databases

InterProiIPR003653. Peptidase_C48_C.
[Graphical view]
PfamiPF02902. Peptidase_C48. 1 hit.
[Graphical view]
PROSITEiPS50600. ULP_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiULP1_YEAST
AccessioniPrimary (citable) accession number: Q02724
Secondary accession number(s): D6W3Z2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 377 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.