Q02685 (RMI1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RecQ-mediated genome instability protein 1

Gene names

Name:	RMI1
Synonyms:	NCE4
Ordered Locus Names:	YPL024W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Structure-specific DNA-binding protein with a preference for cruciform structures. Also binds single-stranded DNA (ssDNA). Functions together with SGS1 and TOP3 to maintain genome integrity. Essential for proper meiotic cell division. Required for normal S-phase progression and DNA damage response. Required for resistance to the DNA-damaging agent methyl methanesulfonate (MMS). Ref.3 Ref.6 Ref.7
Subunit structure	Forms a complex with SGS1 and TOP3. Ref.6 Ref.7
Subcellular location	Cytoplasm. Nucleus Ref.4.
Miscellaneous	Present with 1500 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the RMI1 family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage Meiosis
Cellular component	Cytoplasm Nucleus
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW meiosis Inferred from electronic annotation. Source: UniProtKB-KW mitotic sister chromatid cohesion Inferred from mutant phenotype. Source: SGD positive regulation of DNA binding Inferred from direct assay. Source: SGD response to DNA damage stimulus Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	RecQ helicase-Topo III complex Inferred from direct assay Ref.7. Source: SGD cytoplasm Inferred from direct assay Ref.4. Source: SGD nucleus Inferred from direct assay Ref.4. Source: SGD
Molecular function	enzyme activator activity Inferred from direct assay. Source: SGD four-way junction DNA binding Inferred from direct assay Ref.7. Source: SGD single-stranded DNA binding Inferred from direct assay Ref.7. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 241	241	RecQ-mediated genome instability protein 1		PRO_0000270571
Amino acid modifications
	Modified residue	98	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q02685 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 65EF152F50BF0084	FASTA	241	26,860
	10 20 30 40 50 60 MSFSSILSQD ITDDITPPAY SATLGSREQI VFRAYQNEPW LAGTASNLIL DKKLVIVDRE 70 80 90 100 110 120 LLFQVLMVEN ITKSKLTQID DIKTKLDPKK QKVDRLRSGA QGNGAKKYEV ITQVDMEDDG 130 140 150 160 170 180 NVADNNCAKE NNSNNNSSAA KNKAVFKLTL QSKSGDVFFA INSTPISWSS CMLGSKIVIL 190 200 210 220 230 240 PGTVFNRGVF ILKDSQVIFL GGINRVWNEN RDQKFCDYLE SKLQRDKQLV NGGSKKRKAN D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"A genome-wide screen for methyl methanesulfonate-sensitive mutants reveals genes required for S phase progression in the presence of DNA damage." Chang M., Bellaoui M., Boone C., Brown G.W. Proc. Natl. Acad. Sci. U.S.A. 99:16934-16939(2002) [PubMed: 12482937] [Abstract] Cited for: FUNCTION IN MMS RESISTANCE.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"RMI1/NCE4, a suppressor of genome instability, encodes a member of the RecQ helicase/Topo III complex." Chang M., Bellaoui M., Zhang C., Desai R., Morozov P., Delgado-Cruzata L., Rothstein R., Freyer G.A., Boone C., Brown G.W. EMBO J. 24:2024-2033(2005) [PubMed: 15889139] [Abstract] Cited for: FUNCTION, INTERACTION WITH SGS1 AND TOP3.
[7]	"Yeast Rmi1/Nce4 controls genome stability as a subunit of the Sgs1-Top3 complex." Mullen J.R., Nallaseth F.S., Lan Y.Q., Slagle C.E., Brill S.J. Mol. Cell. Biol. 25:4476-4487(2005) [PubMed: 15899853] [Abstract] Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH SGS1 AND TOP3.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-98, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U36624 Genomic DNA. Translation: AAB68163.1. BK006949 Genomic DNA. Translation: DAA11404.1.
PIR	S63458.
RefSeq	NP_015301.1. NM_001183838.1.
3D structure databases
ProteinModelPortal	Q02685.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4039N.
IntAct	Q02685. 1 interaction.
MINT	MINT-488977.
STRING	Q02685.
Proteomic databases
PeptideAtlas	Q02685.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL024W; YPL024W; YPL024W.
GeneID	856083.
KEGG	sce:YPL024W.
NMPDR	fig|4932.3.peg.6435.
Organism-specific databases
CYGD	YPL024w.
SGD	S000005945. RMI1.
Phylogenomic databases
eggNOG	fuNOG10931.
HOGENOM	HBG203931.
OMA	LLFQVCM.
OrthoDB	EOG4897X6.
Gene expression databases
ArrayExpress	Q02685.
Genevestigator	Q02685.
Family and domain databases
KO	K15364.
ProtoNet	Search...
Other
NextBio	981095.

Entry information

Entry name

RMI1_YEAST

Accession

Primary (citable) accession number: Q02685
Secondary accession number(s): D6W3Y8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program