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Protein

tRNA (uracil-O(2)-)-methyltransferase

Gene

TRM44

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

tRNA (uracil-O2-)-methyltransferase, which catalyzes the formation of O2-methyluracil at position 44 (Um44) in tRNA(Ser).1 Publication

Miscellaneous

Present with 7720 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + uridine(44) in tRNA(Ser) = S-adenosyl-L-homocysteine + 2'-O-methyluridine(44) in tRNA(Ser).

GO - Molecular functioni

GO - Biological processi

  • tRNA methylation Source: SGD

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:G3O-33945-MONOMER
YEAST:G3O-33945-MONOMER
BRENDAi2.1.1.211 984

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (uracil-O(2)-)-methyltransferase (EC:2.1.1.211)
Alternative name(s):
tRNA(Ser) (uridine(44)-2'-O)-methyltransferase
Short name:
tRNA Um(44) 2'-O-methyltransferase
Gene namesi
Name:TRM44
Ordered Locus Names:YPL030W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL030W
SGDiS000005951 TRM44

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002499111 – 567tRNA (uracil-O(2)-)-methyltransferaseAdd BLAST567

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei107PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ02648
PaxDbiQ02648
PRIDEiQ02648

PTM databases

iPTMnetiQ02648

Interactioni

Protein-protein interaction databases

BioGridi36148, 111 interactors
IntActiQ02648, 9 interactors
STRINGi4932.YPL030W

Structurei

3D structure databases

ProteinModelPortaliQ02648
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TRM44 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000000645
HOGENOMiHOG000248551
InParanoidiQ02648
KOiK15447
OMAiPGFVDIG
OrthoDBiEOG092C150W

Family and domain databases

InterProiView protein in InterPro
IPR011671 tRNA_uracil_MeTrfase
PfamiView protein in Pfam
PF07757 AdoMet_MTase, 1 hit

Sequencei

Sequence statusi: Complete.

Q02648-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGDGSAHIS KNNQNQHKDR FKFIVNDKSI LGPQWLSLYQ TDGKVTFAKS
60 70 80 90 100
HFEQAMMNVI REPNINSTVI LRADILKEIN HAAEAGSEPK FDESVLKKFE
110 120 130 140 150
IDNGNESGEE DVKKINIEDL NIRSCETSES LKLSPVHEFV RRIIPRNFYK
160 170 180 190 200
DAIINQTCLI LNSKDPNFQE TSLIVYTPHI NSEKDCPFYI PRTQSVGILL
210 220 230 240 250
HQSVLSVHYI PFPEDKTAFT DESERVVRTA YRLLQTANKH SKGVMQGYEK
260 270 280 290 300
RVNHDQVVNK VNFQNTYIVL KKKYSKFLVE NWAESTDPKK HVFEDIAIAA
310 320 330 340 350
FLIELWIKVY GPDFRSKMQF RDLGCGNGAL CYILLSESIK GLGIDARKRK
360 370 380 390 400
SWSIYPPEVQ SSLKEQVIIP SILLRPHPAL KRQVPHLEHN GRFFPVKVTH
410 420 430 440 450
EVIAPATVVY SSEDLLKSPQ VNTAEFPPDT FIIGNHSDEL TCWIPLLGHP
460 470 480 490 500
YMVIPCCSHN FSGQRVRFNV RKRSPRSNEI KNQNNSKSTY SGLVDHVEYI
510 520 530 540 550
SSRVGWKVEK EMLRIPSTRN AAIIGVENAT LKHFPTQAVY DMIWEDGGAE
560
GWIQNTMSLL KRNPRNH
Length:567
Mass (Da):64,855
Last modified:November 1, 1996 - v1
Checksum:i5374D45014C047C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36624 Genomic DNA Translation: AAB68157.1
U44030 Genomic DNA Translation: AAB68189.1
BK006949 Genomic DNA Translation: DAA11399.1
PIRiS63452
RefSeqiNP_015295.1, NM_001183844.1

Genome annotation databases

EnsemblFungiiYPL030W; YPL030W; YPL030W
GeneIDi856077
KEGGisce:YPL030W

Similar proteinsi

Entry informationi

Entry nameiTRM44_YEAST
AccessioniPrimary (citable) accession number: Q02648
Secondary accession number(s): D6W3Y3, Q03090, Q2XN15
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health