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Q02644 (GHRHR_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Growth hormone-releasing hormone receptor

Short name=GHRH receptor
Alternative name(s):
Growth hormone-releasing factor receptor
Short name=GRF receptor
Short name=GRFR
Gene names
Name:Ghrhr
Synonyms:Grfr
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length464 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor for GRF, coupled to G proteins which activate adenylyl cyclase. Stimulates somatotroph cell growth, growth hormone gene transcription and growth hormone secretion.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Pituitary gland.

Sequence similarities

Belongs to the G-protein coupled receptor 2 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactivation of adenylate cyclase activity

Inferred from direct assay PubMed 10465288PubMed 9845677. Source: RGD

adenylate cyclase-activating G-protein coupled receptor signaling pathway

Inferred from direct assay PubMed 10465288. Source: RGD

adenylate cyclase-modulating G-protein coupled receptor signaling pathway

Inferred from direct assay PubMed 10465288. Source: RGD

cAMP-mediated signaling

Inferred from electronic annotation. Source: Ensembl

cell maturation

Inferred from electronic annotation. Source: Ensembl

cellular response to insulin stimulus

Inferred from electronic annotation. Source: Ensembl

determination of adult lifespan

Inferred from electronic annotation. Source: Ensembl

growth hormone secretion

Inferred from electronic annotation. Source: Ensembl

hormone metabolic process

Inferred from electronic annotation. Source: Ensembl

lactation

Inferred from electronic annotation. Source: Ensembl

positive regulation of cAMP metabolic process

Inferred from direct assay PubMed 10465288. Source: RGD

positive regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of growth hormone secretion

Inferred from electronic annotation. Source: Ensembl

positive regulation of multicellular organism growth

Inferred from electronic annotation. Source: Ensembl

regulation of growth

Traceable author statement PubMed 11735246. Source: RGD

regulation of insulin-like growth factor receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

regulation of intracellular steroid hormone receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

regulation of protein metabolic process

Inferred from electronic annotation. Source: Ensembl

response to estrogen

Inferred from electronic annotation. Source: Ensembl

response to glucocorticoid

Inferred from electronic annotation. Source: Ensembl

signal transduction

Inferred from direct assay PubMed 12446584. Source: RGD

somatotropin secreting cell development

Inferred from electronic annotation. Source: Ensembl

water homeostasis

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcell surface

Inferred from direct assay PubMed 10465288. Source: RGD

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

nuclear inner membrane

Inferred from electronic annotation. Source: Ensembl

nuclear matrix

Inferred from electronic annotation. Source: Ensembl

nuclear outer membrane

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from direct assay PubMed 10465288PubMed 15499571. Source: RGD

secretory granule

Inferred from electronic annotation. Source: Ensembl

   Molecular_functiongrowth hormone-releasing hormone receptor activity

Inferred from direct assay PubMed 10465288PubMed 11814616PubMed 12161265. Source: RGD

peptide hormone binding

Inferred from direct assay PubMed 10465288. Source: RGD

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: Q02644-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: Q02644-2)

The sequence of this isoform differs from the canonical sequence as follows:
     325-365: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 464442Growth hormone-releasing hormone receptor
PRO_0000012831

Regions

Topological domain23 – 132110Extracellular Potential
Transmembrane133 – 15220Helical; Name=1; Potential
Topological domain153 – 16210Cytoplasmic Potential
Transmembrane163 – 18119Helical; Name=2; Potential
Topological domain182 – 20423Extracellular Potential
Transmembrane205 – 22723Helical; Name=3; Potential
Topological domain228 – 24013Cytoplasmic Potential
Transmembrane241 – 26222Helical; Name=4; Potential
Topological domain263 – 28018Extracellular Potential
Transmembrane281 – 30424Helical; Name=5; Potential
Topological domain305 – 37066Cytoplasmic Potential
Transmembrane371 – 38919Helical; Name=6; Potential
Topological domain390 – 40213Extracellular Potential
Transmembrane403 – 42220Helical; Name=7; Potential
Topological domain423 – 46442Cytoplasmic Potential

Amino acid modifications

Glycosylation501N-linked (GlcNAc...) Potential
Disulfide bond41 ↔ 64 By similarity
Disulfide bond55 ↔ 96 By similarity
Disulfide bond78 ↔ 112 By similarity

Natural variations

Alternative sequence325 – 36541Missing in isoform Short.
VSP_002003

Experimental info

Sequence conflict2331S → W in L07380. Ref.2
Sequence conflict3051C → S in L07380. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified June 1, 1994. Version 2.
Checksum: 5FFF49A7D79A4114

FASTA46451,522
        10         20         30         40         50         60 
MDSLLWATWV LCLLNLWGVA LGHLHLECDF ITQLRDDELA CLQAAEGTNN SSMGCPGTWD 

        70         80         90        100        110        120 
GLLCWPPTGS GQWVSLPCPE FFSHFGSDPG AVKRDCTITG WSDPFPPYPV ACPVPLELLT 

       130        140        150        160        170        180 
EEKSYFSTVK IIYTTGHSIS IVALCVAIAI LVALRRLHCP RNYIHTQLFA TFILKASAVF 

       190        200        210        220        230        240 
LKDAAVFQGD STDHCSMSTI LCKVSVAVSH FATMTNFSWL LAEAVYLSCL LASTSPRSKP 

       250        260        270        280        290        300 
AFWWLVLAGW GLPVLCTGTW VGCKLAFEDT ACWDLDDSSP YWWIIKGPIV LSVGVNFGLF 

       310        320        330        340        350        360 
LNIICILLRK LGPAQGGLHT RAQYCNYLLP WSCPLPQVPR ERTDLGPSSH EITVQESGTR 

       370        380        390        400        410        420 
NCQLPWRLSK STLLLIPLFG IHYIIFNFLP DSAGLGIRLP LELGLGSFQG FVVAVLYCFL 

       430        440        450        460 
NQEVRTEISR KWYGHDPELL PARRTCTEWT TPPRSRVKVL TSEC 

« Hide

Isoform Short [UniParc].

Checksum: 79F335DB3E92A912
Show »

FASTA42346,917

References

[1]"Molecular cloning and expression of a pituitary-specific receptor for growth hormone-releasing hormone."
Mayo K.E.
Mol. Endocrinol. 6:1734-1744(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Pituitary.
[2]"Pit-1-dependent expression of the receptor for growth hormone releasing factor mediates pituitary cell growth."
Lin C.R., Lin S.-C., Chang C.P., Rosenfeld M.G.
Nature 360:765-768(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L01407 mRNA. Translation: AAA41221.1.
L07380 mRNA. No translation available.
PIRS29754.
RefSeqNP_036982.1. NM_012850.1. [Q02644-2]
UniGeneRn.10936.

3D structure databases

ProteinModelPortalQ02644.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000016087.

Chemistry

BindingDBQ02644.
ChEMBLCHEMBL3709.
GuidetoPHARMACOLOGY247.

Protein family/group databases

GPCRDBSearch...

Proteomic databases

PRIDEQ02644.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000016087; ENSRNOP00000016087; ENSRNOG00000011808. [Q02644-1]
ENSRNOT00000044578; ENSRNOP00000043884; ENSRNOG00000011808. [Q02644-2]
GeneID25321.
KEGGrno:25321.
UCSCRGD:2688. rat. [Q02644-1]

Organism-specific databases

CTD2692.
RGD2688. Ghrhr.

Phylogenomic databases

eggNOGNOG249002.
GeneTreeENSGT00740000115231.
HOGENOMHOG000008249.
HOVERGENHBG008318.
InParanoidQ02644.
KOK04584.
OMAIHYIIFN.
OrthoDBEOG7TF78W.
PhylomeDBQ02644.
TreeFamTF315710.

Gene expression databases

GenevestigatorQ02644.

Family and domain databases

InterProIPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR003288. GPCR_2_GHRH_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
[Graphical view]
PfamPF00002. 7tm_2. 1 hit.
PF02793. HRM. 1 hit.
[Graphical view]
PRINTSPR01352. GHRHRECEPTOR.
PR00249. GPCRSECRETIN.
SMARTSM00008. HormR. 1 hit.
[Graphical view]
PROSITEPS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio606165.
PROQ02644.

Entry information

Entry nameGHRHR_RAT
AccessionPrimary (citable) accession number: Q02644
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: June 1, 1994
Last modified: June 11, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries