Q02644 (GHRHR_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Growth hormone-releasing hormone receptor
Short name=GHRH receptor

Alternative name(s):
Growth hormone-releasing factor receptor
Short name=GRF receptor
Short name=GRFR

Gene names

Name:	Ghrhr
Synonyms:	Grfr

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for GRF, coupled to G proteins which activate adenylyl cyclase. Stimulates somatotroph cell growth, growth hormone gene transcription and growth hormone secretion.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Pituitary gland.
Sequence similarities	Belongs to the G-protein coupled receptor 2 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of adenylate cyclase activity Inferred from direct assay PubMed 10465288PubMed 9845677. Source: RGD adenylate cyclase-activating G-protein coupled receptor signaling pathway Inferred from direct assay PubMed 10465288. Source: RGD cAMP-mediated signaling Inferred from electronic annotation. Source: Compara cell maturation Inferred from electronic annotation. Source: Compara cellular response to insulin stimulus Inferred from electronic annotation. Source: Compara determination of adult lifespan Inferred from electronic annotation. Source: Compara growth hormone secretion Inferred from electronic annotation. Source: Compara hormone metabolic process Inferred from electronic annotation. Source: Compara lactation Inferred from electronic annotation. Source: Compara positive regulation of cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of growth hormone secretion Inferred from electronic annotation. Source: Compara positive regulation of multicellular organism growth Inferred from electronic annotation. Source: Compara regulation of growth Traceable author statement PubMed 11735246. Source: RGD regulation of insulin-like growth factor receptor signaling pathway Inferred from electronic annotation. Source: Compara regulation of intracellular steroid hormone receptor signaling pathway Inferred from electronic annotation. Source: Compara regulation of protein metabolic process Inferred from electronic annotation. Source: Compara response to estrogen stimulus Inferred from electronic annotation. Source: Compara response to glucocorticoid stimulus Inferred from electronic annotation. Source: Compara somatotropin secreting cell development Inferred from electronic annotation. Source: Compara water homeostasis Inferred from electronic annotation. Source: Compara
Cellular_component	cell surface Inferred from direct assay PubMed 10465288. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nuclear inner membrane Inferred from electronic annotation. Source: Compara nuclear matrix Inferred from electronic annotation. Source: Compara nuclear outer membrane Inferred from electronic annotation. Source: Compara plasma membrane Inferred from direct assay PubMed 10465288PubMed 15499571. Source: RGD secretory granule Inferred from electronic annotation. Source: Compara
Molecular_function	growth hormone-releasing hormone receptor activity Inferred from direct assay PubMed 10465288PubMed 11814616PubMed 12161265. Source: RGD peptide hormone binding Inferred from direct assay PubMed 10465288. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: Q02644-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: Q02644-2)

The sequence of this isoform differs from the canonical sequence as follows:
     325-365: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 464	442	Growth hormone-releasing hormone receptor		PRO_0000012831
Regions
	Topological domain	23 – 132	110	Extracellular Potential
	Transmembrane	133 – 152	20	Helical; Name=1; Potential
	Topological domain	153 – 162	10	Cytoplasmic Potential
	Transmembrane	163 – 181	19	Helical; Name=2; Potential
	Topological domain	182 – 204	23	Extracellular Potential
	Transmembrane	205 – 227	23	Helical; Name=3; Potential
	Topological domain	228 – 240	13	Cytoplasmic Potential
	Transmembrane	241 – 262	22	Helical; Name=4; Potential
	Topological domain	263 – 280	18	Extracellular Potential
	Transmembrane	281 – 304	24	Helical; Name=5; Potential
	Topological domain	305 – 370	66	Cytoplasmic Potential
	Transmembrane	371 – 389	19	Helical; Name=6; Potential
	Topological domain	390 – 402	13	Extracellular Potential
	Transmembrane	403 – 422	20	Helical; Name=7; Potential
	Topological domain	423 – 464	42	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	50	1	N-linked (GlcNAc...) Potential
	Disulfide bond	41 ↔ 64		By similarity
	Disulfide bond	55 ↔ 96		By similarity
	Disulfide bond	78 ↔ 112		By similarity
Natural variations
	Alternative sequence	325 – 365	41	Missing in isoform Short.		VSP_002003
Experimental info
	Sequence conflict	233	1	S → W in L07380. Ref.2
	Sequence conflict	305	1	C → S in L07380. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified June 1, 1994. Version 2. Checksum: 5FFF49A7D79A4114	FASTA	464	51,522
	10 20 30 40 50 60 MDSLLWATWV LCLLNLWGVA LGHLHLECDF ITQLRDDELA CLQAAEGTNN SSMGCPGTWD 70 80 90 100 110 120 GLLCWPPTGS GQWVSLPCPE FFSHFGSDPG AVKRDCTITG WSDPFPPYPV ACPVPLELLT 130 140 150 160 170 180 EEKSYFSTVK IIYTTGHSIS IVALCVAIAI LVALRRLHCP RNYIHTQLFA TFILKASAVF 190 200 210 220 230 240 LKDAAVFQGD STDHCSMSTI LCKVSVAVSH FATMTNFSWL LAEAVYLSCL LASTSPRSKP 250 260 270 280 290 300 AFWWLVLAGW GLPVLCTGTW VGCKLAFEDT ACWDLDDSSP YWWIIKGPIV LSVGVNFGLF 310 320 330 340 350 360 LNIICILLRK LGPAQGGLHT RAQYCNYLLP WSCPLPQVPR ERTDLGPSSH EITVQESGTR 370 380 390 400 410 420 NCQLPWRLSK STLLLIPLFG IHYIIFNFLP DSAGLGIRLP LELGLGSFQG FVVAVLYCFL 430 440 450 460 NQEVRTEISR KWYGHDPELL PARRTCTEWT TPPRSRVKVL TSEC
	Isoform Short [UniParc]. Checksum: 79F335DB3E92A912	FASTA	423	46,917
	10 20 30 40 50 60 MDSLLWATWV LCLLNLWGVA LGHLHLECDF ITQLRDDELA CLQAAEGTNN SSMGCPGTWD 70 80 90 100 110 120 GLLCWPPTGS GQWVSLPCPE FFSHFGSDPG AVKRDCTITG WSDPFPPYPV ACPVPLELLT 130 140 150 160 170 180 EEKSYFSTVK IIYTTGHSIS IVALCVAIAI LVALRRLHCP RNYIHTQLFA TFILKASAVF 190 200 210 220 230 240 LKDAAVFQGD STDHCSMSTI LCKVSVAVSH FATMTNFSWL LAEAVYLSCL LASTSPRSKP 250 260 270 280 290 300 AFWWLVLAGW GLPVLCTGTW VGCKLAFEDT ACWDLDDSSP YWWIIKGPIV LSVGVNFGLF 310 320 330 340 350 360 LNIICILLRK LGPAQGGLHT RAQYWRLSKS TLLLIPLFGI HYIIFNFLPD SAGLGIRLPL 370 380 390 400 410 420 ELGLGSFQGF VVAVLYCFLN QEVRTEISRK WYGHDPELLP ARRTCTEWTT PPRSRVKVLT SEC

References

[1]	"Molecular cloning and expression of a pituitary-specific receptor for growth hormone-releasing hormone." Mayo K.E. Mol. Endocrinol. 6:1734-1744(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pituitary.
[2]	"Pit-1-dependent expression of the receptor for growth hormone releasing factor mediates pituitary cell growth." Lin C.R., Lin S.-C., Chang C.P., Rosenfeld M.G. Nature 360:765-768(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L01407 mRNA. Translation: AAA41221.1. L07380 mRNA. No translation available.
IPI	IPI00231958. IPI00562354.
PIR	S29754.
RefSeq	NP_036982.1. NM_012850.1.
UniGene	Rn.10936.
3D structure databases
ProteinModelPortal	Q02644.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000016087.
Protein family/group databases
GPCRDB	Search...
Proteomic databases
PRIDE	Q02644.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000016087; ENSRNOP00000016087; ENSRNOG00000011808. ENSRNOT00000044578; ENSRNOP00000043884; ENSRNOG00000011808.
GeneID	25321.
KEGG	rno:25321.
UCSC	RGD:2688. rat.
Organism-specific databases
CTD	2692.
RGD	2688. Ghrhr.
Phylogenomic databases
eggNOG	NOG249002.
GeneTree	ENSGT00620000087538.
HOGENOM	HOG000008249.
HOVERGEN	HBG008318.
InParanoid	Q02644.
KO	K04584.
Gene expression databases
ArrayExpress	Q02644.
Genevestigator	Q02644.
GermOnline	ENSRNOG00000011808. Rattus norvegicus.
Family and domain databases
InterPro	IPR017981. GPCR_2-like. IPR001879. GPCR_2_extracellular_dom. IPR003288. GPCR_2_GHRH_rcpt. IPR000832. GPCR_2_secretin-like. IPR017983. GPCR_2_secretin-like_CS. [Graphical view]
PANTHER	PTHR12011:SF26. PTHR12011:SF26. 1 hit.
Pfam	PF00002. 7tm_2. 1 hit. PF02793. HRM. 1 hit. [Graphical view]
PRINTS	PR01352. GHRHRECEPTOR. PR00249. GPCRSECRETIN.
SMART	SM00008. HormR. 1 hit. [Graphical view]
PROSITE	PS00649. G_PROTEIN_RECEP_F2_1. 1 hit. PS00650. G_PROTEIN_RECEP_F2_2. 1 hit. PS50227. G_PROTEIN_RECEP_F2_3. 1 hit. PS50261. G_PROTEIN_RECEP_F2_4. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q02644.
ChEMBL	CHEMBL3709.
NextBio	606165.

Entry information

Entry name

GHRHR_RAT

Accession

Primary (citable) accession number: Q02644

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program