Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Growth hormone-releasing hormone receptor

Gene

Ghrhr

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for GRF, coupled to G proteins which activate adenylyl cyclase. Stimulates somatotroph cell growth, growth hormone gene transcription and growth hormone secretion.

GO - Molecular functioni

  1. growth hormone-releasing hormone receptor activity Source: RGD
  2. peptide hormone binding Source: RGD

GO - Biological processi

  1. activation of adenylate cyclase activity Source: RGD
  2. adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: RGD
  3. adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: RGD
  4. cAMP-mediated signaling Source: Ensembl
  5. cell maturation Source: Ensembl
  6. cellular response to insulin stimulus Source: Ensembl
  7. determination of adult lifespan Source: Ensembl
  8. growth hormone secretion Source: Ensembl
  9. hormone metabolic process Source: Ensembl
  10. lactation Source: Ensembl
  11. positive regulation of cAMP metabolic process Source: RGD
  12. positive regulation of cell proliferation Source: Ensembl
  13. positive regulation of growth hormone secretion Source: Ensembl
  14. positive regulation of multicellular organism growth Source: Ensembl
  15. regulation of growth Source: RGD
  16. regulation of insulin-like growth factor receptor signaling pathway Source: Ensembl
  17. regulation of intracellular steroid hormone receptor signaling pathway Source: Ensembl
  18. regulation of protein metabolic process Source: Ensembl
  19. response to estrogen Source: Ensembl
  20. response to glucocorticoid Source: Ensembl
  21. signal transduction Source: RGD
  22. somatotropin secreting cell development Source: Ensembl
  23. water homeostasis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_235881. Glucagon-type ligand receptors.
REACT_246814. G alpha (s) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Growth hormone-releasing hormone receptor
Short name:
GHRH receptor
Alternative name(s):
Growth hormone-releasing factor receptor
Short name:
GRF receptor
Short name:
GRFR
Gene namesi
Name:Ghrhr
Synonyms:Grfr
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi2688. Ghrhr.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 132110ExtracellularSequence AnalysisAdd
BLAST
Transmembranei133 – 15220Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini153 – 16210CytoplasmicSequence Analysis
Transmembranei163 – 18119Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini182 – 20423ExtracellularSequence AnalysisAdd
BLAST
Transmembranei205 – 22723Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini228 – 24013CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei241 – 26222Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini263 – 28018ExtracellularSequence AnalysisAdd
BLAST
Transmembranei281 – 30424Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini305 – 37066CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei371 – 38919Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini390 – 40213ExtracellularSequence AnalysisAdd
BLAST
Transmembranei403 – 42220Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini423 – 46442CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell surface Source: RGD
  2. integral component of membrane Source: UniProtKB-KW
  3. nuclear inner membrane Source: Ensembl
  4. nuclear matrix Source: Ensembl
  5. nuclear outer membrane Source: Ensembl
  6. plasma membrane Source: RGD
  7. secretory granule Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 464442Growth hormone-releasing hormone receptorPRO_0000012831Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi41 ↔ 64By similarity
Glycosylationi50 – 501N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi55 ↔ 96By similarity
Disulfide bondi78 ↔ 112By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ02644.

Expressioni

Tissue specificityi

Pituitary gland.

Gene expression databases

GenevestigatoriQ02644.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016087.

Structurei

3D structure databases

ProteinModelPortaliQ02644.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG249002.
GeneTreeiENSGT00760000118800.
HOGENOMiHOG000008249.
HOVERGENiHBG008318.
InParanoidiQ02644.
KOiK04584.
OMAiIHYIIFN.
OrthoDBiEOG7TF78W.
PhylomeDBiQ02644.
TreeFamiTF315710.

Family and domain databases

InterProiIPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR003288. GPCR_2_GHRH_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF02793. HRM. 1 hit.
[Graphical view]
PRINTSiPR01352. GHRHRECEPTOR.
PR00249. GPCRSECRETIN.
SMARTiSM00008. HormR. 1 hit.
[Graphical view]
PROSITEiPS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: Q02644-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDSLLWATWV LCLLNLWGVA LGHLHLECDF ITQLRDDELA CLQAAEGTNN
60 70 80 90 100
SSMGCPGTWD GLLCWPPTGS GQWVSLPCPE FFSHFGSDPG AVKRDCTITG
110 120 130 140 150
WSDPFPPYPV ACPVPLELLT EEKSYFSTVK IIYTTGHSIS IVALCVAIAI
160 170 180 190 200
LVALRRLHCP RNYIHTQLFA TFILKASAVF LKDAAVFQGD STDHCSMSTI
210 220 230 240 250
LCKVSVAVSH FATMTNFSWL LAEAVYLSCL LASTSPRSKP AFWWLVLAGW
260 270 280 290 300
GLPVLCTGTW VGCKLAFEDT ACWDLDDSSP YWWIIKGPIV LSVGVNFGLF
310 320 330 340 350
LNIICILLRK LGPAQGGLHT RAQYCNYLLP WSCPLPQVPR ERTDLGPSSH
360 370 380 390 400
EITVQESGTR NCQLPWRLSK STLLLIPLFG IHYIIFNFLP DSAGLGIRLP
410 420 430 440 450
LELGLGSFQG FVVAVLYCFL NQEVRTEISR KWYGHDPELL PARRTCTEWT
460
TPPRSRVKVL TSEC
Length:464
Mass (Da):51,522
Last modified:June 1, 1994 - v2
Checksum:i5FFF49A7D79A4114
GO
Isoform Short (identifier: Q02644-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     325-365: Missing.

Show »
Length:423
Mass (Da):46,917
Checksum:i79F335DB3E92A912
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti233 – 2331S → W in L07380. (PubMed:1334535)Curated
Sequence conflicti305 – 3051C → S in L07380. (PubMed:1334535)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei325 – 36541Missing in isoform Short. CuratedVSP_002003Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01407 mRNA. Translation: AAA41221.1.
L07380 mRNA. No translation available.
PIRiS29754.
RefSeqiNP_036982.1. NM_012850.1. [Q02644-2]
UniGeneiRn.10936.

Genome annotation databases

EnsembliENSRNOT00000016087; ENSRNOP00000016087; ENSRNOG00000011808. [Q02644-1]
ENSRNOT00000044578; ENSRNOP00000043884; ENSRNOG00000011808. [Q02644-2]
GeneIDi25321.
KEGGirno:25321.
UCSCiRGD:2688. rat. [Q02644-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01407 mRNA. Translation: AAA41221.1.
L07380 mRNA. No translation available.
PIRiS29754.
RefSeqiNP_036982.1. NM_012850.1. [Q02644-2]
UniGeneiRn.10936.

3D structure databases

ProteinModelPortaliQ02644.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016087.

Chemistry

BindingDBiQ02644.
ChEMBLiCHEMBL3709.
GuidetoPHARMACOLOGYi247.

Protein family/group databases

GPCRDBiSearch...

Proteomic databases

PRIDEiQ02644.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000016087; ENSRNOP00000016087; ENSRNOG00000011808. [Q02644-1]
ENSRNOT00000044578; ENSRNOP00000043884; ENSRNOG00000011808. [Q02644-2]
GeneIDi25321.
KEGGirno:25321.
UCSCiRGD:2688. rat. [Q02644-1]

Organism-specific databases

CTDi2692.
RGDi2688. Ghrhr.

Phylogenomic databases

eggNOGiNOG249002.
GeneTreeiENSGT00760000118800.
HOGENOMiHOG000008249.
HOVERGENiHBG008318.
InParanoidiQ02644.
KOiK04584.
OMAiIHYIIFN.
OrthoDBiEOG7TF78W.
PhylomeDBiQ02644.
TreeFamiTF315710.

Enzyme and pathway databases

ReactomeiREACT_235881. Glucagon-type ligand receptors.
REACT_246814. G alpha (s) signalling events.

Miscellaneous databases

NextBioi606165.
PROiQ02644.

Gene expression databases

GenevestigatoriQ02644.

Family and domain databases

InterProiIPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR003288. GPCR_2_GHRH_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF02793. HRM. 1 hit.
[Graphical view]
PRINTSiPR01352. GHRHRECEPTOR.
PR00249. GPCRSECRETIN.
SMARTiSM00008. HormR. 1 hit.
[Graphical view]
PROSITEiPS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and expression of a pituitary-specific receptor for growth hormone-releasing hormone."
    Mayo K.E.
    Mol. Endocrinol. 6:1734-1744(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pituitary.
  2. "Pit-1-dependent expression of the receptor for growth hormone releasing factor mediates pituitary cell growth."
    Lin C.R., Lin S.-C., Chang C.P., Rosenfeld M.G.
    Nature 360:765-768(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiGHRHR_RAT
AccessioniPrimary (citable) accession number: Q02644
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: June 1, 1994
Last modified: January 7, 2015
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.