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Protein

Nascent polypeptide-associated complex subunit beta-1

Gene

EGD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the nascent polypeptide-associated complex (NAC), a dynamic component of the ribosomal exit tunnel, protecting the emerging polypeptides from interaction with other cytoplasmic proteins to ensure appropriate nascent protein targeting. The NAC complex also promotes mitochondrial protein import by enhancing productive ribosome interactions with the outer mitochondrial membrane and blocks the inappropriate interaction of ribosomes translating non-secretory nascent polypeptides with translocation sites in the membrane of the endoplasmic reticulum EGD1 may act as a transcription factor that exert a negative effect on the expression of several genes that are transcribed by RNA polymerase II. Can enhance DNA binding of the GAL4 protein activator.3 Publications

GO - Molecular functioni

  • unfolded protein binding Source: SGD

GO - Biological processi

  • 'de novo' cotranslational protein folding Source: SGD
  • macroautophagy Source: SGD
  • posttranslational protein targeting to membrane Source: SGD
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Protein transport, Transcription, Transcription regulation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33951-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nascent polypeptide-associated complex subunit beta-1
Short name:
NAC-beta-1
Alternative name(s):
BTF3 homolog EGD1
Beta-1-NAC
GAL4 DNA-binding enhancer protein 1
Gene namesi
Name:EGD1
Ordered Locus Names:YPL037C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL037C.
SGDiS000005958. EGD1.

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: Predominantly cytoplasmic, may also transiently localize to the nucleus.

GO - Cellular componenti

  • nascent polypeptide-associated complex Source: SGD
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002135521 – 157Nascent polypeptide-associated complex subunit beta-1Add BLAST157

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei151PhosphothreonineCombined sources1

Post-translational modificationi

Ubiquitinated by the NOT4 E3 ligase and the UBC4 E2 ubiquitin conjugatin enzyme.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ02642.
PRIDEiQ02642.
TopDownProteomicsiQ02642.

PTM databases

iPTMnetiQ02642.

Interactioni

Subunit structurei

Part of the nascent polypeptide-associated complex (NAC), consisting of EGD2 and either EGD1 or BTT1. NAC associates with ribosomes via EGD1 or BTT1, and with the CCR4-NOT complex.

Binary interactionsi

WithEntry#Exp.IntActNotes
EGD2P388797EBI-6371,EBI-6379
RPL25P044564EBI-6371,EBI-15308

GO - Molecular functioni

  • unfolded protein binding Source: SGD

Protein-protein interaction databases

BioGridi36142. 84 interactors.
DIPiDIP-6561N.
IntActiQ02642. 14 interactors.
MINTiMINT-688536.

Structurei

3D structure databases

ProteinModelPortaliQ02642.
SMRiQ02642.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 103NAC-A/BPROSITE-ProRule annotationAdd BLAST66

Sequence similaritiesi

Belongs to the NAC-beta family.Curated
Contains 1 NAC-A/B (NAC-alpha/beta) domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000006283.
HOGENOMiHOG000261245.
InParanoidiQ02642.
KOiK01527.
OMAiVQGANDY.
OrthoDBiEOG092C58TX.

Family and domain databases

InterProiIPR002715. Nas_poly-pep-assoc_cplx_dom.
[Graphical view]
PfamiPF01849. NAC. 1 hit.
[Graphical view]
SMARTiSM01407. NAC. 1 hit.
[Graphical view]
PROSITEiPS51151. NAC_AB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q02642-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIDQEKLAK LQKLSANNKV GGTRRKLNKK AGSSAGANKD DTKLQSQLAK
60 70 80 90 100
LHAVTIDNVA EANFFKDDGK VMHFNKVGVQ VAAQHNTSVF YGLPQEKNLQ
110 120 130 140 150
DLFPGIISQL GPEAIQALSQ LAAQMEKHEA KAPADAEKKD EAIPELVEGQ

TFDADVE
Length:157
Mass (Da):17,020
Last modified:February 1, 1995 - v2
Checksum:i7C278A3DC79BF95B
GO

Sequence cautioni

The sequence AAB24290 differs from that shown. Reason: Frameshift at position 80.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05185 Genomic DNA. Translation: AAB63973.1. Frameshift.
S49596 Genomic DNA. Translation: AAB24290.1. Frameshift.
X78725 Genomic DNA. Translation: CAA55371.1.
U44030 Genomic DNA. Translation: AAB68183.1.
AY558440 Genomic DNA. Translation: AAS56766.1.
J04421 Genomic DNA. No translation available.
BK006949 Genomic DNA. Translation: DAA11393.1.
PIRiS47575.
RefSeqiNP_015288.1. NM_001183851.1.

Genome annotation databases

EnsemblFungiiYPL037C; YPL037C; YPL037C.
GeneIDi856070.
KEGGisce:YPL037C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05185 Genomic DNA. Translation: AAB63973.1. Frameshift.
S49596 Genomic DNA. Translation: AAB24290.1. Frameshift.
X78725 Genomic DNA. Translation: CAA55371.1.
U44030 Genomic DNA. Translation: AAB68183.1.
AY558440 Genomic DNA. Translation: AAS56766.1.
J04421 Genomic DNA. No translation available.
BK006949 Genomic DNA. Translation: DAA11393.1.
PIRiS47575.
RefSeqiNP_015288.1. NM_001183851.1.

3D structure databases

ProteinModelPortaliQ02642.
SMRiQ02642.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36142. 84 interactors.
DIPiDIP-6561N.
IntActiQ02642. 14 interactors.
MINTiMINT-688536.

PTM databases

iPTMnetiQ02642.

Proteomic databases

MaxQBiQ02642.
PRIDEiQ02642.
TopDownProteomicsiQ02642.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL037C; YPL037C; YPL037C.
GeneIDi856070.
KEGGisce:YPL037C.

Organism-specific databases

EuPathDBiFungiDB:YPL037C.
SGDiS000005958. EGD1.

Phylogenomic databases

GeneTreeiENSGT00390000006283.
HOGENOMiHOG000261245.
InParanoidiQ02642.
KOiK01527.
OMAiVQGANDY.
OrthoDBiEOG092C58TX.

Enzyme and pathway databases

BioCyciYEAST:G3O-33951-MONOMER.

Miscellaneous databases

PROiQ02642.

Family and domain databases

InterProiIPR002715. Nas_poly-pep-assoc_cplx_dom.
[Graphical view]
PfamiPF01849. NAC. 1 hit.
[Graphical view]
SMARTiSM01407. NAC. 1 hit.
[Graphical view]
PROSITEiPS51151. NAC_AB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNACB1_YEAST
AccessioniPrimary (citable) accession number: Q02642
Secondary accession number(s): D6W3X7, Q02107
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 154 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 18000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.