Reviewed, UniProtKB/Swiss-Prot Q02635 (AATA_RHIME)

Names and origin

Protein names

Recommended name:
    Aspartate aminotransferase A
      Short name=AspAT
    EC=2.6.1.1
Alternative name(s):
    Transaminase A

Gene names

Name:	aatA
Ordered Locus Names:	R02325
ORF Names:	SMc01578

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Required for symbiotic nitrogen fixation.
Catalytic activity	L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.
Cofactor	Pyridoxal phosphate.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-aminocyclopropane-1-carboxylate synthase activity Inferred from electronic annotation. Source: InterPro L-aspartate:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 400	400	Aspartate aminotransferase A		PRO_0000123847
Amino acid modifications
	Modified residue	239	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q02635-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 34668FAE196660D6	FASTA	400	43,583
	10 20 30 40 50 60 MAFLADALSR VKPSATIAVS QKARELKAKG RDVIGLGAGE PDFDTPDNIK KAAIDAIDRG 70 80 90 100 110 120 ETKYTPVSGI PELREAIAKK FKRENNLDYT AAQTIVGTGG KQILFNAFMA TLNPGDEVVI 130 140 150 160 170 180 PAPYWVSYPE MVALCGGTPV FVPTRQENNF KLKAEDLDRA ITPKTKWFVF NSPSNPSGAA 190 200 210 220 230 240 YSHEELKALT DVLMKHPHVW VLTDDMYEHL TYGDFRFATP VEVEPGLYER TLTMNGVSKA 250 260 270 280 290 300 YAMTGWRIGY AAGPLHLIKA MDMIQGQQTS GAASIAQWAA VEALNGPQDF IGRNKEIFQG 310 320 330 340 350 360 RRDLVVSMLN QAKGISCPTP EGAFYVYPSC AGLIGKTAPS GKVIETDEDF VSELLETEGV 370 380 390 400 AVVHGSAFGL GPNFRISYAT SEALLEEACR RIQRFCAACR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Aspartate aminotransferase activity is required for aspartate catabolism and symbiotic nitrogen fixation in Rhizobium meliloti." Rastogi V.K., Watson R.J. J. Bacteriol. 173:2879-2887(1991) [PubMed: 2019560] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: JJ1c10.
[2]	"Cloning and nucleotide sequencing of Rhizobium meliloti aminotransferase genes: an aspartate aminotransferase required for symbiotic nitrogen fixation is atypical." Watson R.J., Rastogi V.K. J. Bacteriol. 175:1919-1928(1993) [PubMed: 8096210] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: JJ1c10.
[3]	"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L05064 Genomic DNA. Translation: AAA26245.1. AL591688 Genomic DNA. Translation: CAC46904.1.
PIR	A47094.
RefSeq	NP_386431.1.
3D structure databases
HSSP	HSSP built from PDB template 1BJW based on UniProtKB Q56232.
ModBase	Search...
Genome annotation databases
GeneID	1233992.
GenomeReviews	Gene locus R02325 in contig AL591688_GR.
KEGG	sme:SMc01578.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q02635.
OMA	IGKTAPS.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC01578-MON.
BRENDA	2.6.1.1. 142.
Family and domain databases
InterPro	IPR001176. ACC_synthase. IPR004839. Aminotransferase_I/II. IPR004838. NHTrfase_class1_PyrdxlP-BS. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
PRINTS	PR00753. ACCSYNTHASE.
PROSITE	PS00105. AA_TRANSFER_CLASS_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AATA_RHIME

Accession

Primary (citable) accession number: Q02635

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	November 3, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)