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Protein

Nucleoporin NUP100/NSP100

Gene

NUP100

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). NUP100 plays an important role in several nuclear export and import pathways including poly(A)+ RNA and protein transport.9 Publications

GO - Molecular functioni

  • nuclear localization sequence binding Source: GO_Central
  • nucleocytoplasmic transporter activity Source: SGD
  • RNA binding Source: GO_Central
  • structural constituent of nuclear pore Source: SGD

GO - Biological processi

  • mRNA export from nucleus Source: SGD
  • mRNA export from nucleus in response to heat stress Source: SGD
  • NLS-bearing protein import into nucleus Source: SGD
  • positive regulation of transcription, DNA-templated Source: SGD
  • posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery Source: SGD
  • protein import into nucleus Source: SGD
  • protein targeting to nuclear inner membrane Source: SGD
  • telomere tethering at nuclear periphery Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31864-MONOMER.

Protein family/group databases

MEROPSiS59.A09.
TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin NUP100/NSP100
Alternative name(s):
Nuclear pore protein NUP100/NSP100
Gene namesi
Name:NUP100
Synonyms:NSP100
Ordered Locus Names:YKL068W
ORF Names:YKL336
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL068W.
SGDiS000001551. NUP100.

Subcellular locationi

GO - Cellular componenti

  • nuclear membrane Source: UniProtKB-SubCell
  • nuclear pore Source: SGD
  • nuclear pore central transport channel Source: SGD
  • nuclear pore cytoplasmic filaments Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 959959Nucleoporin NUP100/NSP100PRO_0000204830Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei763 – 7631PhosphoserineCombined sources
Modified residuei783 – 7831PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ02629.

PTM databases

iPTMnetiQ02629.

Interactioni

Subunit structurei

The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEH1 and SEC1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. Through its FG repeats NUP100 interacts with numerous karyopherins including KAP95, and MEX67.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KAP95Q061424EBI-11698,EBI-9145
NUP116Q026305EBI-11698,EBI-11703
NUP42P496862EBI-11698,EBI-12310
NUP57P488373EBI-11698,EBI-12324

Protein-protein interaction databases

BioGridi34065. 88 interactions.
DIPiDIP-2351N.
IntActiQ02629. 26 interactions.
MINTiMINT-519762.

Structurei

3D structure databases

ProteinModelPortaliQ02629.
SMRiQ02629. Positions 816-955.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati2 – 32FG 1
Repeati9 – 102FG 2
Repeati17 – 171FG 3
Repeati21 – 244SLFG 1; approximate
Repeati33 – 364SLFG 2
Repeati51 – 544SLFG 3; approximate
Repeati66 – 694SLFG 4
Repeati77 – 804GLFG 1; approximate
Repeati89 – 924SLFG 5; approximate
Repeati105 – 1062FG 4
Repeati112 – 1154GLFG 2; approximate
Repeati131 – 1344SLFG 6; approximate
Repeati145 – 1462FG 5
Repeati157 – 1604SLFG 7
Repeati168 – 1714GLFG 3; approximate
Repeati175 – 1784SLFG 8; approximate
Repeati189 – 1902FG 6
Repeati202 – 2054SLFG 9
Repeati210 – 2134SLFG 10; approximate
Repeati220 – 2234SLFG 11
Repeati233 – 2342FG 7
Repeati242 – 2454SLFG 12; approximate
Repeati253 – 2564SLFG 13
Repeati271 – 2744GLFG 4
Repeati287 – 2904GLFG 5
Repeati300 – 3034GLFG 6; approximate
Repeati318 – 3214SLFG 14
Repeati333 – 3364GLFG 7
Repeati345 – 3484GLFG 8
Repeati358 – 3614GLFG 9
Repeati379 – 3824GLFG 10
Repeati393 – 3964GLFG 11
Repeati405 – 4084SLFG 15
Repeati417 – 4204SLFG 16
Repeati436 – 4394SLFG 17
Repeati448 – 4492FG 8
Repeati462 – 4654SLFG 18
Repeati474 – 4774SLFG 19; approximate
Repeati490 – 4934GLFG 12
Repeati506 – 5094GLFG 13
Repeati523 – 5264GLFG 14
Repeati542 – 5432FG 9
Repeati550 – 5534GLFG 15
Repeati569 – 5702FG 10
Domaini814 – 956143Peptidase S59PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni816 – 955140Nucleoporin RNA-binding motif (NRM)Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi32 – 386355Asn-richAdd
BLAST
Compositional biasi37 – 437Poly-Asn
Compositional biasi52 – 570519Gly-richAdd
BLAST
Compositional biasi312 – 3176Poly-Asn
Compositional biasi337 – 39054Gln-richAdd
BLAST
Compositional biasi387 – 3904Poly-Gln

Domaini

Contains FG repeats. FG repeats are interaction sites for karyopherins (importins, exportins) and form probably an affinity gradient, guiding the transport proteins unidirectionally with their cargo through the NPC. FG repeat regions are highly flexible and lack ordered secondary structure. The overall conservation of FG repeats regarding exact sequence, spacing, and repeat unit length is limited. FG repeat types and their physico-chemical environment change across the NPC from the nucleoplasmic to the cytoplasmic side: GLFG repeats are especially abundant in NUPs in the central region (lacking a charged environment but are enriched in Ser, Thr, Gln, and Asn).

Sequence similaritiesi

Belongs to the nucleoporin GLFG family.Curated
Contains 1 peptidase S59 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00550000074799.
HOGENOMiHOG000093958.
InParanoidiQ02629.
KOiK14297.
OMAiGMNYGMM.
OrthoDBiEOG092C0BHV.

Family and domain databases

Gene3Di3.30.1610.10. 1 hit.
InterProiIPR025574. Nucleoporin_FG_rpt.
IPR007230. Peptidase_S59.
[Graphical view]
PfamiPF04096. Nucleoporin2. 1 hit.
PF13634. Nucleoporin_FG. 7 hits.
[Graphical view]
SUPFAMiSSF82215. SSF82215. 1 hit.
PROSITEiPS51434. NUP_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q02629-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFGNNRPMFG GSNLSFGSNT SSFGGQQSQQ PNSLFGNSNN NNNSTSNNAQ
60 70 80 90 100
SGFGGFTSAA GSNSNSLFGN NNTQNNGAFG QSMGATQNSP FGSLNSSNAS
110 120 130 140 150
NGNTFGGSSS MGSFGGNTNN AFNNNSNSTN SPFGFNKPNT GGTLFGSQNN
160 170 180 190 200
NSAGTSSLFG GQSTSTTGTF GNTGSSFGTG LNGNGSNIFG AGNNSQSNTT
210 220 230 240 250
GSLFGNQQSS AFGTNNQQGS LFGQQSQNTN NAFGNQNQLG GSSFGSKPVG
260 270 280 290 300
SGSLFGQSNN TLGNTTNNRN GLFGQMNSSN QGSSNSGLFG QNSMNSSTQG
310 320 330 340 350
VFGQNNNQMQ INGNNNNSLF GKANTFSNSA SGGLFGQNNQ QQGSGLFGQN
360 370 380 390 400
SQTSGSSGLF GQNNQKQPNT FTQSNTGIGL FGQNNNQQQQ STGLFGAKPA
410 420 430 440 450
GTTGSLFGGN SSTQPNSLFG TTNVPTSNTQ SQQGNSLFGA TKLTNMPFGG
460 470 480 490 500
NPTANQSGSG NSLFGTKPAS TTGSLFGNNT ASTTVPSTNG LFGNNANNST
510 520 530 540 550
STTNTGLFGA KPDSQSKPAL GGGLFGNSNS NSSTIGQNKP VFGGTTQNTG
560 570 580 590 600
LFGATGTNSS AVGSTGKLFG QNNNTLNVGT QNVPPVNNTT QNALLGTTAV
610 620 630 640 650
PSLQQAPVTN EQLFSKISIP NSITNPVKAT TSKVNADMKR NSSLTSAYRL
660 670 680 690 700
APKPLFAPSS NGDAKFQKWG KTLERSDRGS STSNSITDPE SSYLNSNDLL
710 720 730 740 750
FDPDRRYLKH LVIKNNKNLN VINHNDDEAS KVKLVTFTTE SASKDDQASS
760 770 780 790 800
SIAASKLTEK AHSPQTDLKD DHDESTPDPQ SKSPNGSTSI PMIENEKISS
810 820 830 840 850
KVPGLLSNDV TFFKNNYYIS PSIETLGNKS LIELRKINNL VIGHRNYGKV
860 870 880 890 900
EFLEPVDLLN TPLDTLCGDL VTFGPKSCSI YENCSIKPEK GEGINVRCRV
910 920 930 940 950
TLYSCFPIDK ETRKPIKNIT HPLLKRSIAK LKENPVYKFE SYDPVTGTYS

YTIDHPVLT
Length:959
Mass (Da):99,988
Last modified:July 1, 1993 - v1
Checksum:iD3985F9901BBAA51
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z15035 Genomic DNA. Translation: CAA78753.1.
X75780 Genomic DNA. Translation: CAA53406.1.
Z28068 Genomic DNA. Translation: CAA81905.1.
BK006944 Genomic DNA. Translation: DAA09089.1.
PIRiB44402.
RefSeqiNP_012855.1. NM_001179634.1.

Genome annotation databases

EnsemblFungiiYKL068W; YKL068W; YKL068W.
GeneIDi853796.
KEGGisce:YKL068W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z15035 Genomic DNA. Translation: CAA78753.1.
X75780 Genomic DNA. Translation: CAA53406.1.
Z28068 Genomic DNA. Translation: CAA81905.1.
BK006944 Genomic DNA. Translation: DAA09089.1.
PIRiB44402.
RefSeqiNP_012855.1. NM_001179634.1.

3D structure databases

ProteinModelPortaliQ02629.
SMRiQ02629. Positions 816-955.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34065. 88 interactions.
DIPiDIP-2351N.
IntActiQ02629. 26 interactions.
MINTiMINT-519762.

Protein family/group databases

MEROPSiS59.A09.
TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

PTM databases

iPTMnetiQ02629.

Proteomic databases

MaxQBiQ02629.

Protocols and materials databases

DNASUi853796.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL068W; YKL068W; YKL068W.
GeneIDi853796.
KEGGisce:YKL068W.

Organism-specific databases

EuPathDBiFungiDB:YKL068W.
SGDiS000001551. NUP100.

Phylogenomic databases

GeneTreeiENSGT00550000074799.
HOGENOMiHOG000093958.
InParanoidiQ02629.
KOiK14297.
OMAiGMNYGMM.
OrthoDBiEOG092C0BHV.

Enzyme and pathway databases

BioCyciYEAST:G3O-31864-MONOMER.

Miscellaneous databases

PROiQ02629.

Family and domain databases

Gene3Di3.30.1610.10. 1 hit.
InterProiIPR025574. Nucleoporin_FG_rpt.
IPR007230. Peptidase_S59.
[Graphical view]
PfamiPF04096. Nucleoporin2. 1 hit.
PF13634. Nucleoporin_FG. 7 hits.
[Graphical view]
SUPFAMiSSF82215. SSF82215. 1 hit.
PROSITEiPS51434. NUP_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNU100_YEAST
AccessioniPrimary (citable) accession number: Q02629
Secondary accession number(s): D6VXL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: September 7, 2016
This is version 155 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 358 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.