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Protein

Nucleoporin NUP100/NSP100

Gene

NUP100

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). NUP100 plays an important role in several nuclear export and import pathways including poly(A)+ RNA and protein transport.9 Publications

GO - Molecular functioni

  • nuclear localization sequence binding Source: GO_Central
  • nucleocytoplasmic transporter activity Source: SGD
  • RNA binding Source: GO_Central
  • structural constituent of nuclear pore Source: SGD

GO - Biological processi

  • mRNA export from nucleus Source: SGD
  • mRNA export from nucleus in response to heat stress Source: SGD
  • NLS-bearing protein import into nucleus Source: SGD
  • positive regulation of transcription, DNA-templated Source: SGD
  • posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery Source: SGD
  • protein import into nucleus Source: SGD
  • protein targeting to nuclear inner membrane Source: SGD
  • telomere tethering at nuclear periphery Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31864-MONOMER.

Protein family/group databases

MEROPSiS59.A09.
TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin NUP100/NSP100
Alternative name(s):
Nuclear pore protein NUP100/NSP100
Gene namesi
Name:NUP100
Synonyms:NSP100
Ordered Locus Names:YKL068W
ORF Names:YKL336
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL068W.
SGDiS000001551. NUP100.

Subcellular locationi

GO - Cellular componenti

  • nuclear membrane Source: UniProtKB-SubCell
  • nuclear pore Source: SGD
  • nuclear pore central transport channel Source: SGD
  • nuclear pore cytoplasmic filaments Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002048301 – 959Nucleoporin NUP100/NSP100Add BLAST959

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei763PhosphoserineCombined sources1
Modified residuei783PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ02629.
PRIDEiQ02629.

PTM databases

iPTMnetiQ02629.

Interactioni

Subunit structurei

The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEH1 and SEC1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. Through its FG repeats NUP100 interacts with numerous karyopherins including KAP95, and MEX67.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KAP95Q061424EBI-11698,EBI-9145
NUP116Q026305EBI-11698,EBI-11703
NUP42P496862EBI-11698,EBI-12310
NUP57P488373EBI-11698,EBI-12324
NUP84P528912EBI-11698,EBI-12337
NUP85P466734EBI-11698,EBI-12345

Protein-protein interaction databases

BioGridi34065. 88 interactors.
DIPiDIP-2351N.
IntActiQ02629. 27 interactors.
MINTiMINT-519762.

Structurei

3D structure databases

ProteinModelPortaliQ02629.
SMRiQ02629.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati2 – 3FG 12
Repeati9 – 10FG 22
Repeati17FG 31
Repeati21 – 24SLFG 1; approximate4
Repeati33 – 36SLFG 24
Repeati51 – 54SLFG 3; approximate4
Repeati66 – 69SLFG 44
Repeati77 – 80GLFG 1; approximate4
Repeati89 – 92SLFG 5; approximate4
Repeati105 – 106FG 42
Repeati112 – 115GLFG 2; approximate4
Repeati131 – 134SLFG 6; approximate4
Repeati145 – 146FG 52
Repeati157 – 160SLFG 74
Repeati168 – 171GLFG 3; approximate4
Repeati175 – 178SLFG 8; approximate4
Repeati189 – 190FG 62
Repeati202 – 205SLFG 94
Repeati210 – 213SLFG 10; approximate4
Repeati220 – 223SLFG 114
Repeati233 – 234FG 72
Repeati242 – 245SLFG 12; approximate4
Repeati253 – 256SLFG 134
Repeati271 – 274GLFG 44
Repeati287 – 290GLFG 54
Repeati300 – 303GLFG 6; approximate4
Repeati318 – 321SLFG 144
Repeati333 – 336GLFG 74
Repeati345 – 348GLFG 84
Repeati358 – 361GLFG 94
Repeati379 – 382GLFG 104
Repeati393 – 396GLFG 114
Repeati405 – 408SLFG 154
Repeati417 – 420SLFG 164
Repeati436 – 439SLFG 174
Repeati448 – 449FG 82
Repeati462 – 465SLFG 184
Repeati474 – 477SLFG 19; approximate4
Repeati490 – 493GLFG 124
Repeati506 – 509GLFG 134
Repeati523 – 526GLFG 144
Repeati542 – 543FG 92
Repeati550 – 553GLFG 154
Repeati569 – 570FG 102
Domaini814 – 956Peptidase S59PROSITE-ProRule annotationAdd BLAST143

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni816 – 955Nucleoporin RNA-binding motif (NRM)Add BLAST140

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi32 – 386Asn-richAdd BLAST355
Compositional biasi37 – 43Poly-Asn7
Compositional biasi52 – 570Gly-richAdd BLAST519
Compositional biasi312 – 317Poly-Asn6
Compositional biasi337 – 390Gln-richAdd BLAST54
Compositional biasi387 – 390Poly-Gln4

Domaini

Contains FG repeats. FG repeats are interaction sites for karyopherins (importins, exportins) and form probably an affinity gradient, guiding the transport proteins unidirectionally with their cargo through the NPC. FG repeat regions are highly flexible and lack ordered secondary structure. The overall conservation of FG repeats regarding exact sequence, spacing, and repeat unit length is limited. FG repeat types and their physico-chemical environment change across the NPC from the nucleoplasmic to the cytoplasmic side: GLFG repeats are especially abundant in NUPs in the central region (lacking a charged environment but are enriched in Ser, Thr, Gln, and Asn).

Sequence similaritiesi

Belongs to the nucleoporin GLFG family.Curated
Contains 1 peptidase S59 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00550000074799.
HOGENOMiHOG000093958.
InParanoidiQ02629.
KOiK14297.
OMAiGMNYGMM.
OrthoDBiEOG092C0BHV.

Family and domain databases

Gene3Di3.30.1610.10. 1 hit.
InterProiIPR025574. Nucleoporin_FG_rpt.
IPR007230. Peptidase_S59.
[Graphical view]
PfamiPF04096. Nucleoporin2. 1 hit.
PF13634. Nucleoporin_FG. 4 hits.
[Graphical view]
SUPFAMiSSF82215. SSF82215. 1 hit.
PROSITEiPS51434. NUP_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q02629-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFGNNRPMFG GSNLSFGSNT SSFGGQQSQQ PNSLFGNSNN NNNSTSNNAQ
60 70 80 90 100
SGFGGFTSAA GSNSNSLFGN NNTQNNGAFG QSMGATQNSP FGSLNSSNAS
110 120 130 140 150
NGNTFGGSSS MGSFGGNTNN AFNNNSNSTN SPFGFNKPNT GGTLFGSQNN
160 170 180 190 200
NSAGTSSLFG GQSTSTTGTF GNTGSSFGTG LNGNGSNIFG AGNNSQSNTT
210 220 230 240 250
GSLFGNQQSS AFGTNNQQGS LFGQQSQNTN NAFGNQNQLG GSSFGSKPVG
260 270 280 290 300
SGSLFGQSNN TLGNTTNNRN GLFGQMNSSN QGSSNSGLFG QNSMNSSTQG
310 320 330 340 350
VFGQNNNQMQ INGNNNNSLF GKANTFSNSA SGGLFGQNNQ QQGSGLFGQN
360 370 380 390 400
SQTSGSSGLF GQNNQKQPNT FTQSNTGIGL FGQNNNQQQQ STGLFGAKPA
410 420 430 440 450
GTTGSLFGGN SSTQPNSLFG TTNVPTSNTQ SQQGNSLFGA TKLTNMPFGG
460 470 480 490 500
NPTANQSGSG NSLFGTKPAS TTGSLFGNNT ASTTVPSTNG LFGNNANNST
510 520 530 540 550
STTNTGLFGA KPDSQSKPAL GGGLFGNSNS NSSTIGQNKP VFGGTTQNTG
560 570 580 590 600
LFGATGTNSS AVGSTGKLFG QNNNTLNVGT QNVPPVNNTT QNALLGTTAV
610 620 630 640 650
PSLQQAPVTN EQLFSKISIP NSITNPVKAT TSKVNADMKR NSSLTSAYRL
660 670 680 690 700
APKPLFAPSS NGDAKFQKWG KTLERSDRGS STSNSITDPE SSYLNSNDLL
710 720 730 740 750
FDPDRRYLKH LVIKNNKNLN VINHNDDEAS KVKLVTFTTE SASKDDQASS
760 770 780 790 800
SIAASKLTEK AHSPQTDLKD DHDESTPDPQ SKSPNGSTSI PMIENEKISS
810 820 830 840 850
KVPGLLSNDV TFFKNNYYIS PSIETLGNKS LIELRKINNL VIGHRNYGKV
860 870 880 890 900
EFLEPVDLLN TPLDTLCGDL VTFGPKSCSI YENCSIKPEK GEGINVRCRV
910 920 930 940 950
TLYSCFPIDK ETRKPIKNIT HPLLKRSIAK LKENPVYKFE SYDPVTGTYS

YTIDHPVLT
Length:959
Mass (Da):99,988
Last modified:July 1, 1993 - v1
Checksum:iD3985F9901BBAA51
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z15035 Genomic DNA. Translation: CAA78753.1.
X75780 Genomic DNA. Translation: CAA53406.1.
Z28068 Genomic DNA. Translation: CAA81905.1.
BK006944 Genomic DNA. Translation: DAA09089.1.
PIRiB44402.
RefSeqiNP_012855.1. NM_001179634.1.

Genome annotation databases

EnsemblFungiiYKL068W; YKL068W; YKL068W.
GeneIDi853796.
KEGGisce:YKL068W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z15035 Genomic DNA. Translation: CAA78753.1.
X75780 Genomic DNA. Translation: CAA53406.1.
Z28068 Genomic DNA. Translation: CAA81905.1.
BK006944 Genomic DNA. Translation: DAA09089.1.
PIRiB44402.
RefSeqiNP_012855.1. NM_001179634.1.

3D structure databases

ProteinModelPortaliQ02629.
SMRiQ02629.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34065. 88 interactors.
DIPiDIP-2351N.
IntActiQ02629. 27 interactors.
MINTiMINT-519762.

Protein family/group databases

MEROPSiS59.A09.
TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

PTM databases

iPTMnetiQ02629.

Proteomic databases

MaxQBiQ02629.
PRIDEiQ02629.

Protocols and materials databases

DNASUi853796.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL068W; YKL068W; YKL068W.
GeneIDi853796.
KEGGisce:YKL068W.

Organism-specific databases

EuPathDBiFungiDB:YKL068W.
SGDiS000001551. NUP100.

Phylogenomic databases

GeneTreeiENSGT00550000074799.
HOGENOMiHOG000093958.
InParanoidiQ02629.
KOiK14297.
OMAiGMNYGMM.
OrthoDBiEOG092C0BHV.

Enzyme and pathway databases

BioCyciYEAST:G3O-31864-MONOMER.

Miscellaneous databases

PROiQ02629.

Family and domain databases

Gene3Di3.30.1610.10. 1 hit.
InterProiIPR025574. Nucleoporin_FG_rpt.
IPR007230. Peptidase_S59.
[Graphical view]
PfamiPF04096. Nucleoporin2. 1 hit.
PF13634. Nucleoporin_FG. 4 hits.
[Graphical view]
SUPFAMiSSF82215. SSF82215. 1 hit.
PROSITEiPS51434. NUP_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNU100_YEAST
AccessioniPrimary (citable) accession number: Q02629
Secondary accession number(s): D6VXL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 30, 2016
This is version 158 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 358 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.