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Protein

Beta-galactosidase small subunit

Gene

lacM

Organism
Leuconostoc lactis
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase small subunit (EC:3.2.1.23)
Short name:
Beta-gal small subunit
Alternative name(s):
Lactase small subunit
Gene namesi
Name:lacM
Encoded oniPlasmid pNZ630 Publication
OrganismiLeuconostoc lactis
Taxonomic identifieri1246 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeLeuconostoc

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 319319Beta-galactosidase small subunitPRO_0000057673Add
BLAST

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.

Structurei

3D structure databases

ProteinModelPortaliQ02604.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di2.70.98.10. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR014718. Glyco_hydro-type_carb-bd_sub.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
[Graphical view]
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.

Sequencei

Sequence statusi: Complete.

Q02604-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAYTNNQLHV IYGDGSLGLQ GANFHYLFSY ERGGLESLVV NDKEWLYRTP
60 70 80 90 100
TPMFWRATTD NDHGSGFSVK SAQWYAADKF STCQDIELTV DDQPVTPLPI
110 120 130 140 150
APLNNKYTDH EIATKVSLAY HFVTTTVPST IVTVTYTVTA DGQINIATHY
160 170 180 190 200
SGQSDLPELP AFGLRFIMPT TATGFDYTGL SGETYPDRLA GATHGQFHVD
210 220 230 240 250
SLPVTPYLVP QECGMHMQTE QVTVTRSTTQ NNADHDNTPF SLTFSQTDAP
260 270 280 290 300
FAFSCLPYTA AELENATHME ELPLARRTVL SIYGAVRGVG GIDSWGTDVE
310
APYHILANQD IDFSFNIHF
Length:319
Mass (Da):35,260
Last modified:July 1, 1993 - v1
Checksum:i880794B75AC0F009
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M92281 Genomic DNA. Translation: AAA25268.1.
PIRiB42891.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M92281 Genomic DNA. Translation: AAA25268.1.
PIRiB42891.

3D structure databases

ProteinModelPortaliQ02604.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.70.98.10. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR014718. Glyco_hydro-type_carb-bd_sub.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
[Graphical view]
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Leuconostoc lactis beta-galactosidase is encoded by two overlapping genes."
    David S., Stevens H., van Riel M., Simons G., de Vos W.M.
    J. Bacteriol. 174:4475-4481(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
    Strain: NZ6009.

Entry informationi

Entry nameiBGAM_LEULA
AccessioniPrimary (citable) accession number: Q02604
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: October 1, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Plasmid

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.