Reviewed, UniProtKB/Swiss-Prot Q02603 (BGAL_LEULA)

Names and origin

Protein names

Recommended name:
    Beta-galactosidase large subunit
      Short name=Beta-gal large subunit
    EC=3.2.1.23
Alternative name(s):
    Lactase large subunit

Gene names

Name:

lacL

Encoded on

Plasmid pNZ63

Organism

Leuconostoc lactis

Taxonomic identifier

1246 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Leuconostoc

Protein attributes

Sequence length	626 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Subunit structure	Heterodimer of a large and a small subunit.
Sequence similarities	Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 626	626	Beta-galactosidase large subunit		PRO_0000057672
Sites
	Active site	466	1	Proton donor By similarity
	Active site	534	1	Nucleophile By similarity
	Site	318	1	Has an effect on thermostability Potential

Sequences

	Sequence		Length	Mass (Da)
	Q02603-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: E9CEA154B992CF7D	FASTA	626	72,113
	10 20 30 40 50 60 MQANLQWLDD PEVFRVNQLP AHSDHHYYHD TAEFKTGSRF IKSLNGAWRF NFAKTPAERP 70 80 90 100 110 120 VDFYQPDFDA TDFDTIQVPG HIELAGYGQI QYINTLYPWE GKIYRRPPYT LNQDQLTPGL 130 140 150 160 170 180 FSDAADNTVG SYLKTFDLDD VFKGQRIIIQ FQGVEEALYV WLNGHFIGYS EDSFTPSEFD 190 200 210 220 230 240 LTPYIQDQGN VLAVRVYKHS TAAFIEDQDM FRFSGIFRDV NILAEPASHI TDLDIRPVPN 250 260 270 280 290 300 ANLKSGELNI TTKVTGEPAT LALTVKDHDG RVLTSQTQTG SGSVTFDTML FDQLHLWSPQ 310 320 330 340 350 360 TPYLYQLTIE VYDADHQLLE VVPYQFGFRT VELRDDKVIY VNNKRLVING VNRHEWNAHT 370 380 390 400 410 420 GRVISMADMR ADIQTMLANN INADRTCHYP DQLPWYQLCD EAGIYLMAET NLESHGSWQK 430 440 450 460 470 480 MGAIEPSYNV PGDNPHWPAA VIDRARSNYE WFKNHPSIIF WSLGNESYAG EDIAAMQAFY 490 500 510 520 530 540 KEHDDSRLVH YEGVFYTPEL KDRISDVESR MYEKPQNIVA YLEDNPTKPF LNCEYMHDMG 550 560 570 580 590 600 NSLGGMQSYN DLIDKYPMYQ GGFIWDFIDQ ALFVHDPITD QDVLRYGGDF DERHSDYAFS 610 620 GNGLMFADRT PKPAMQEVKY YYGLHK

References

[1]	"Leuconostoc lactis beta-galactosidase is encoded by two overlapping genes." David S., Stevens H., van Riel M., Simons G., de Vos W.M. J. Bacteriol. 174:4475-4481(1992) [PubMed: 1624440] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-6. Strain: NZ6009.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M92281 Genomic DNA. Translation: AAA25267.1.
PIR	A42891.
3D structure databases
HSSP	HSSP built from PDB template 1JZ8 based on UniProtKB P00722.
ModBase	Search...
Protein family/group databases
CAZy	GH2. Glycoside Hydrolase Family 2.
Enzyme and pathway databases
BRENDA	3.2.1.23. 1194.
Family and domain databases
InterPro	IPR006101. Glyco_hydro_2. IPR013812. Glyco_hydro_2/20_Ig-like. IPR006104. Glyco_hydro_2_carb-bd. IPR006102. Glyco_hydro_2_Ig-like. IPR006103. Glyco_hydro_2_TIM. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00703. Glyco_hydro_2. 1 hit. PF02836. Glyco_hydro_2_C. 1 hit. PF02837. Glyco_hydro_2_N. 1 hit. [Graphical view]
PRINTS	PR00132. GLHYDRLASE2.
PROSITE	PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit. PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL_LEULA

Accession

Primary (citable) accession number: Q02603

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)