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Q02603 (BGAL_LEULA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase large subunit

Short name=Beta-gal large subunit
EC=3.2.1.23
Alternative name(s):
Lactase large subunit
Gene names
Name:lacL
Encoded onPlasmid pNZ63
OrganismLeuconostoc lactis
Taxonomic identifier1246 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeLeuconostoc

Protein attributes

Sequence length626 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subunit structure

Heterodimer of a large and a small subunit.

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
   Molecular functionGlycosidase
Hydrolase
   Technical termDirect protein sequencing
Plasmid
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 626626Beta-galactosidase large subunit
PRO_0000057672

Sites

Active site4661Proton donor By similarity
Active site5341Nucleophile By similarity
Site3181Has an effect on thermostability Potential

Sequences

Sequence LengthMass (Da)Tools
Q02603 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: E9CEA154B992CF7D

FASTA62672,113
        10         20         30         40         50         60 
MQANLQWLDD PEVFRVNQLP AHSDHHYYHD TAEFKTGSRF IKSLNGAWRF NFAKTPAERP 

        70         80         90        100        110        120 
VDFYQPDFDA TDFDTIQVPG HIELAGYGQI QYINTLYPWE GKIYRRPPYT LNQDQLTPGL 

       130        140        150        160        170        180 
FSDAADNTVG SYLKTFDLDD VFKGQRIIIQ FQGVEEALYV WLNGHFIGYS EDSFTPSEFD 

       190        200        210        220        230        240 
LTPYIQDQGN VLAVRVYKHS TAAFIEDQDM FRFSGIFRDV NILAEPASHI TDLDIRPVPN 

       250        260        270        280        290        300 
ANLKSGELNI TTKVTGEPAT LALTVKDHDG RVLTSQTQTG SGSVTFDTML FDQLHLWSPQ 

       310        320        330        340        350        360 
TPYLYQLTIE VYDADHQLLE VVPYQFGFRT VELRDDKVIY VNNKRLVING VNRHEWNAHT 

       370        380        390        400        410        420 
GRVISMADMR ADIQTMLANN INADRTCHYP DQLPWYQLCD EAGIYLMAET NLESHGSWQK 

       430        440        450        460        470        480 
MGAIEPSYNV PGDNPHWPAA VIDRARSNYE WFKNHPSIIF WSLGNESYAG EDIAAMQAFY 

       490        500        510        520        530        540 
KEHDDSRLVH YEGVFYTPEL KDRISDVESR MYEKPQNIVA YLEDNPTKPF LNCEYMHDMG 

       550        560        570        580        590        600 
NSLGGMQSYN DLIDKYPMYQ GGFIWDFIDQ ALFVHDPITD QDVLRYGGDF DERHSDYAFS 

       610        620 
GNGLMFADRT PKPAMQEVKY YYGLHK 

« Hide

References

[1]"Leuconostoc lactis beta-galactosidase is encoded by two overlapping genes."
David S., Stevens H., van Riel M., Simons G., de Vos W.M.
J. Bacteriol. 174:4475-4481(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-6.
Strain: NZ6009.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M92281 Genomic DNA. Translation: AAA25267.1.
PIRA42891.

3D structure databases

ProteinModelPortalQ02603.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 1 hit.
InterProIPR008979. Galactose-bd-like.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SUPFAMSSF49303. SSF49303. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_LEULA
AccessionPrimary (citable) accession number: Q02603
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: July 9, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries