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Protein

Beta-galactosidase large subunit

Gene

lacL

Organism
Leuconostoc lactis
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei318 – 3181Has an effect on thermostabilitySequence Analysis
Active sitei466 – 4661Proton donorBy similarity
Active sitei534 – 5341NucleophileBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase large subunit (EC:3.2.1.23)
Short name:
Beta-gal large subunit
Alternative name(s):
Lactase large subunit
Gene namesi
Name:lacL
Encoded oniPlasmid pNZ630 Publication
OrganismiLeuconostoc lactis
Taxonomic identifieri1246 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeLeuconostoc

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 626626Beta-galactosidase large subunitPRO_0000057672Add
BLAST

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.

Structurei

3D structure databases

ProteinModelPortaliQ02603.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SUPFAMiSSF49303. SSF49303. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q02603-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQANLQWLDD PEVFRVNQLP AHSDHHYYHD TAEFKTGSRF IKSLNGAWRF
60 70 80 90 100
NFAKTPAERP VDFYQPDFDA TDFDTIQVPG HIELAGYGQI QYINTLYPWE
110 120 130 140 150
GKIYRRPPYT LNQDQLTPGL FSDAADNTVG SYLKTFDLDD VFKGQRIIIQ
160 170 180 190 200
FQGVEEALYV WLNGHFIGYS EDSFTPSEFD LTPYIQDQGN VLAVRVYKHS
210 220 230 240 250
TAAFIEDQDM FRFSGIFRDV NILAEPASHI TDLDIRPVPN ANLKSGELNI
260 270 280 290 300
TTKVTGEPAT LALTVKDHDG RVLTSQTQTG SGSVTFDTML FDQLHLWSPQ
310 320 330 340 350
TPYLYQLTIE VYDADHQLLE VVPYQFGFRT VELRDDKVIY VNNKRLVING
360 370 380 390 400
VNRHEWNAHT GRVISMADMR ADIQTMLANN INADRTCHYP DQLPWYQLCD
410 420 430 440 450
EAGIYLMAET NLESHGSWQK MGAIEPSYNV PGDNPHWPAA VIDRARSNYE
460 470 480 490 500
WFKNHPSIIF WSLGNESYAG EDIAAMQAFY KEHDDSRLVH YEGVFYTPEL
510 520 530 540 550
KDRISDVESR MYEKPQNIVA YLEDNPTKPF LNCEYMHDMG NSLGGMQSYN
560 570 580 590 600
DLIDKYPMYQ GGFIWDFIDQ ALFVHDPITD QDVLRYGGDF DERHSDYAFS
610 620
GNGLMFADRT PKPAMQEVKY YYGLHK
Length:626
Mass (Da):72,113
Last modified:June 30, 1993 - v1
Checksum:iE9CEA154B992CF7D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M92281 Genomic DNA. Translation: AAA25267.1.
PIRiA42891.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M92281 Genomic DNA. Translation: AAA25267.1.
PIRiA42891.

3D structure databases

ProteinModelPortaliQ02603.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SUPFAMiSSF49303. SSF49303. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Leuconostoc lactis beta-galactosidase is encoded by two overlapping genes."
    David S., Stevens H., van Riel M., Simons G., de Vos W.M.
    J. Bacteriol. 174:4475-4481(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-6.
    Strain: NZ6009.

Entry informationi

Entry nameiBGAL_LEULA
AccessioniPrimary (citable) accession number: Q02603
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 30, 1993
Last sequence update: June 30, 1993
Last modified: September 30, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Plasmid

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.