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Q02603

- BGAL_LEULA

UniProt

Q02603 - BGAL_LEULA

Protein

Beta-galactosidase large subunit

Gene

lacL

Organism
Leuconostoc lactis
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei318 – 3181Has an effect on thermostabilitySequence Analysis
    Active sitei466 – 4661Proton donorBy similarity
    Active sitei534 – 5341NucleophileBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase large subunit (EC:3.2.1.23)
    Short name:
    Beta-gal large subunit
    Alternative name(s):
    Lactase large subunit
    Gene namesi
    Name:lacL
    Encoded oniPlasmid pNZ630 Publication
    OrganismiLeuconostoc lactis
    Taxonomic identifieri1246 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeLeuconostoc

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 626626Beta-galactosidase large subunitPRO_0000057672Add
    BLAST

    Interactioni

    Subunit structurei

    Heterodimer of a large and a small subunit.

    Structurei

    3D structure databases

    ProteinModelPortaliQ02603.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.Curated

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR008979. Galactose-bd-like.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SUPFAMiSSF49303. SSF49303. 1 hit.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q02603-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQANLQWLDD PEVFRVNQLP AHSDHHYYHD TAEFKTGSRF IKSLNGAWRF    50
    NFAKTPAERP VDFYQPDFDA TDFDTIQVPG HIELAGYGQI QYINTLYPWE 100
    GKIYRRPPYT LNQDQLTPGL FSDAADNTVG SYLKTFDLDD VFKGQRIIIQ 150
    FQGVEEALYV WLNGHFIGYS EDSFTPSEFD LTPYIQDQGN VLAVRVYKHS 200
    TAAFIEDQDM FRFSGIFRDV NILAEPASHI TDLDIRPVPN ANLKSGELNI 250
    TTKVTGEPAT LALTVKDHDG RVLTSQTQTG SGSVTFDTML FDQLHLWSPQ 300
    TPYLYQLTIE VYDADHQLLE VVPYQFGFRT VELRDDKVIY VNNKRLVING 350
    VNRHEWNAHT GRVISMADMR ADIQTMLANN INADRTCHYP DQLPWYQLCD 400
    EAGIYLMAET NLESHGSWQK MGAIEPSYNV PGDNPHWPAA VIDRARSNYE 450
    WFKNHPSIIF WSLGNESYAG EDIAAMQAFY KEHDDSRLVH YEGVFYTPEL 500
    KDRISDVESR MYEKPQNIVA YLEDNPTKPF LNCEYMHDMG NSLGGMQSYN 550
    DLIDKYPMYQ GGFIWDFIDQ ALFVHDPITD QDVLRYGGDF DERHSDYAFS 600
    GNGLMFADRT PKPAMQEVKY YYGLHK 626
    Length:626
    Mass (Da):72,113
    Last modified:July 1, 1993 - v1
    Checksum:iE9CEA154B992CF7D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M92281 Genomic DNA. Translation: AAA25267.1.
    PIRiA42891.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M92281 Genomic DNA. Translation: AAA25267.1 .
    PIRi A42891.

    3D structure databases

    ProteinModelPortali Q02603.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR008979. Galactose-bd-like.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SUPFAMi SSF49303. SSF49303. 1 hit.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Leuconostoc lactis beta-galactosidase is encoded by two overlapping genes."
      David S., Stevens H., van Riel M., Simons G., de Vos W.M.
      J. Bacteriol. 174:4475-4481(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-6.
      Strain: NZ6009.

    Entry informationi

    Entry nameiBGAL_LEULA
    AccessioniPrimary (citable) accession number: Q02603
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing, Plasmid

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3