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Protein

Heavy metal tolerance protein

Gene

hmt1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in metal tolerance. Probably involved in the transport of metal-bound phytochelatins. Compartmentalizes cadmium within vacuoles, thereby protecting cells from cadmium toxicity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei542 – 5421Glutathione; via amide nitrogenBy similarity
Binding sitei593 – 5931ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi617 – 62812ATPPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • ATPase activity, coupled to transmembrane movement of substances Source: PomBase
  • ATP binding Source: UniProtKB-KW
  • phytochelatin transmembrane transporter ATPase activity Source: PomBase

GO - Biological processi

  • cadmium ion import into vacuole Source: PomBase
  • cellular detoxification of cadmium ion Source: PomBase
  • cellular iron ion homeostasis Source: GO_Central
  • glutathione transmembrane import into vacuole Source: PomBase
  • phytochelatin 2 import into vacuole Source: PomBase
  • phytochelatin import into vacuole Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Cadmium resistance, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-1369007. Mitochondrial ABC transporters.

Protein family/group databases

TCDBi3.A.1.210.2. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Heavy metal tolerance protein
Gene namesi
Name:hmt1
ORF Names:SPCC737.09c, SPCC74.08c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC737.09c.
PomBaseiSPCC737.09c. hmt1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei51 – 7121HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei88 – 10821HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei126 – 14621HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei156 – 17621HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei263 – 28321HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei304 – 32421HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei381 – 40121HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei403 – 42321HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei490 – 51122HelicalPROSITE-ProRule annotationAdd
BLAST

GO - Cellular componenti

  • fungal-type vacuole membrane Source: PomBase
  • integral component of fungal-type vacuolar membrane Source: PomBase
  • mitochondrial inner membrane Source: GO_Central
  • vacuole Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Add
BLAST
Chaini28 – 830803Heavy metal tolerance proteinPRO_0000000259Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi150 – 1501N-linked (GlcNAc...)Sequence analysis
Glycosylationi350 – 3501N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ02592.

Interactioni

Protein-protein interaction databases

BioGridi280210. 18 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ02592.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini265 – 550286ABC transmembrane type-1PROSITE-ProRule annotationAdd
BLAST
Domaini584 – 818235ABC transporterPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni429 – 4335Glutathione bindingBy similarity
Regioni492 – 4954Glutathione bindingBy similarity

Sequence similaritiesi

Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation
Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiQ02592.
OMAiYILYRWL.
OrthoDBiEOG092C3H79.
PhylomeDBiQ02592.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02592-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLRYNSPRL NILELVLLYV GFFSIGSLNL LQKRKATSDP YRRKNRFGKE
60 70 80 90 100
PIGIISWWIL GIALTYVVDI SNLVIYALRV PNWWPCKTTV VCLILFLLFW
110 120 130 140 150
IIVLISCADS KALPKNADSI LKAYRLSVLY VWAIDIVFET IFIVYSPHPN
160 170 180 190 200
ETFQGIVLAD HVARLVLCVF ATAIYLTYRR KRHTHDPLDF EERQLTEESN
210 220 230 240 250
VNENAISQNP STVQLGVSAS TSNFGTLKST SKKPSDKSWA EYFRSFSTLL
260 270 280 290 300
PYLWPTKDYR LQFQIFICIV LLFLGRAVNI LAPRQLGVLT EKLTKHSEKI
310 320 330 340 350
PWSDVILFVI YRFLQGNMGV IGSLRSFLWV PVSQYAYRAI STKALRHVLN
360 370 380 390 400
LSYDFHLNKR AGEVLTALTK GSSLNTFAEQ VVFQIGPVLL DLGVAMVYFF
410 420 430 440 450
IKFDIYFTLI VLIMTLCYCY VTVKITSWRT EARRKMVNSW RESYAVQNDA
460 470 480 490 500
IMNFETVKNF DADDFENERY GHAVDIYLKQ ERKVLFSLNF LNIVQGGIFT
510 520 530 540 550
FSLAIACLLS AYRVTFGFNT VGDFVILLTY MIQLQQPLNF FGTLYRSLQN
560 570 580 590 600
SIIDTERLLE IFEEKPTVVE KPNAPDLKVT QGKVIFSHVS FAYDPRKPVL
610 620 630 640 650
SDINFVAQPG KVIALVGESG GGKSTIMRIL LRFFDVNSGS ITIDDQDIRN
660 670 680 690 700
VTLSSLRSSI GVVPQDSTLF NDTILYNIKY AKPSATNEEI YAAAKAAQIH
710 720 730 740 750
DRILQFPDGY NSRVGERGLK LSGGEKQRVA VARAILKDPS IILLDEATSA
760 770 780 790 800
LDTNTERQIQ AALNRLASGR TAIVIAHRLS TITNADLILC ISNGRIVETG
810 820 830
THEELIKRDG GAYKKMWFQQ AMGKTSAETH
Length:830
Mass (Da):93,994
Last modified:December 15, 1998 - v3
Checksum:i909FBD10D51F50A9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti79 – 791R → A in CAA78419 (PubMed:1396551).Curated
Sequence conflicti439 – 4391S → T in CAA78419 (PubMed:1396551).Curated
Sequence conflicti812 – 8121A → R in CAA78419 (PubMed:1396551).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14055 mRNA. Translation: CAA78419.1.
CU329672 Genomic DNA. Translation: CAA20865.1.
PIRiS25198.
RefSeqiNP_588371.3. NM_001023362.3.

Genome annotation databases

EnsemblFungiiSPCC737.09c.1; SPCC737.09c.1:pep; SPCC737.09c.
GeneIDi3361134.
KEGGispo:SPCC737.09c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14055 mRNA. Translation: CAA78419.1.
CU329672 Genomic DNA. Translation: CAA20865.1.
PIRiS25198.
RefSeqiNP_588371.3. NM_001023362.3.

3D structure databases

ProteinModelPortaliQ02592.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi280210. 18 interactions.

Protein family/group databases

TCDBi3.A.1.210.2. the atp-binding cassette (abc) superfamily.

Proteomic databases

MaxQBiQ02592.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC737.09c.1; SPCC737.09c.1:pep; SPCC737.09c.
GeneIDi3361134.
KEGGispo:SPCC737.09c.

Organism-specific databases

EuPathDBiFungiDB:SPCC737.09c.
PomBaseiSPCC737.09c. hmt1.

Phylogenomic databases

InParanoidiQ02592.
OMAiYILYRWL.
OrthoDBiEOG092C3H79.
PhylomeDBiQ02592.

Enzyme and pathway databases

ReactomeiR-SPO-1369007. Mitochondrial ABC transporters.

Miscellaneous databases

PROiQ02592.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHMT1_SCHPO
AccessioniPrimary (citable) accession number: Q02592
Secondary accession number(s): O13675, Q9UQW7, Q9USI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: December 15, 1998
Last modified: September 7, 2016
This is version 134 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.