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Protein

[Protein ADP-ribosylarginine] hydrolase

Gene

Adprh

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reverse reaction of mono-ADP-ribosylation.

Catalytic activityi

Protein-N(omega)-(ADP-D-ribosyl)-L-arginine + H2O = ADP-D-ribose + protein-L-arginine.
N(omega)-(ADP-D-ribosyl)-L-arginine + H2O = ADP-D-ribose + L-arginine.

Enzyme regulationi

Its activity is synergistically stimulated by magnesium and dithiothreitol (DTT) in vitro.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi29Potassium; via carbonyl oxygenBy similarity1
Metal bindingi30Potassium; via carbonyl oxygenBy similarity1
Metal bindingi32Potassium; via carbonyl oxygenBy similarity1
Metal bindingi59MagnesiumBy similarity1
Metal bindingi60MagnesiumBy similarity1
Metal bindingi61MagnesiumBy similarity1
Binding sitei90ADP-D-riboseBy similarity1
Binding sitei129ADP-D-ribose; via carbonyl oxygenBy similarity1
Binding sitei135ADP-D-ribose; via amide nitrogenBy similarity1
Metal bindingi308PotassiumBy similarity1
Metal bindingi309MagnesiumBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi106 – 108ADP-D-riboseBy similarity3
Nucleotide bindingi168 – 170ADP-D-riboseBy similarity3
Nucleotide bindingi268 – 269ADP-D-riboseBy similarity2
Nucleotide bindingi274 – 275ADP-D-riboseBy similarity2

GO - Molecular functioni

GO - Biological processi

  • cellular protein modification process Source: UniProtKB
  • protein de-ADP-ribosylation Source: InterPro

Keywordsi

Molecular functionHydrolase
LigandMagnesium, Metal-binding, Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
[Protein ADP-ribosylarginine] hydrolase (EC:3.2.2.19)
Short name:
ADP-ribosylarginine hydrolase
Alternative name(s):
ADP-ribose-L-arginine cleaving enzyme
Gene namesi
Name:Adprh
Synonyms:Arh1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 11

Organism-specific databases

RGDi2052. Adprh.

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001572851 – 362[Protein ADP-ribosylarginine] hydrolaseAdd BLAST362

Proteomic databases

PaxDbiQ02589.
PRIDEiQ02589.

PTM databases

iPTMnetiQ02589.
PhosphoSitePlusiQ02589.

Expressioni

Gene expression databases

BgeeiENSRNOG00000027260.
GenevisibleiQ02589. RN.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000034815.

Structurei

3D structure databases

ProteinModelPortaliQ02589.
SMRiQ02589.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ADP-ribosylglycohydrolase family.Curated

Phylogenomic databases

eggNOGiENOG410IEA9. Eukaryota.
ENOG410XPXD. LUCA.
GeneTreeiENSGT00530000063627.
HOGENOMiHOG000007974.
HOVERGENiHBG050462.
InParanoidiQ02589.
KOiK01245.
OMAiPPRQWGK.
OrthoDBiEOG091G0AJ4.
PhylomeDBiQ02589.
TreeFamiTF329417.

Family and domain databases

Gene3Di1.10.4080.10. 1 hit.
InterProiView protein in InterPro
IPR012108. ADP-ribosylarg_hydro.
IPR005502. Ribosyl_crysJ1.
PfamiView protein in Pfam
PF03747. ADP_ribosyl_GH. 1 hit.
PIRSFiPIRSF016939. ADP_ribslarg_hdr. 1 hit.
SUPFAMiSSF101478. SSF101478. 1 hit.

Sequencei

Sequence statusi: Complete.

Q02589-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGGLIERYV AAMVLSAAGD TLGYFNGKWE FLRDGEKIHR QLAQMGDLEA
60 70 80 90 100
IDVAQWRVSD DTIMHLATAE ALMEAGSSPD LPQLYSLLAK HYRDCMGDMD
110 120 130 140 150
GRAPGGACMQ NAMQLDPDRA DGWRIPFNSH EGGCGAAMRA MCIGLRFPHP
160 170 180 190 200
SQLDTLIQVS IESGRMTHHH PTGYLGSLAS ALFTAYAVNG KSPRQWGKGL
210 220 230 240 250
MEVLPEAKAY VTQSGYFVKE NLQHWSYFEK EWEKYLELRG ILDGKSAPVF
260 270 280 290 300
PKPFGVKERD QFYIEVSYSG WGGSSGHDAP MIAYDALLAA GDSWKELAHR
310 320 330 340 350
AFFHGGDSDS TATIAGCWWG VMHGFKGVNP SNYEKLEYRQ RLEEAGRALY
360
SLGSKEDTIL GP
Length:362
Mass (Da):39,961
Last modified:May 29, 2007 - v2
Checksum:i6C941A940AEAE10E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti252K → Q in AAA40691 (PubMed:1375222).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86341 mRNA. Translation: AAA40691.1.
BC082065 mRNA. Translation: AAH82065.1.
PIRiA38135.
RefSeqiNP_899154.2. NM_183325.2.
XP_006248422.1. XM_006248360.3.
UniGeneiRn.13315.

Genome annotation databases

EnsembliENSRNOT00000038439; ENSRNOP00000034815; ENSRNOG00000027260.
GeneIDi25371.
KEGGirno:25371.
UCSCiRGD:2052. rat.

Similar proteinsi

Entry informationi

Entry nameiADPRH_RAT
AccessioniPrimary (citable) accession number: Q02589
Secondary accession number(s): Q66H27
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: May 29, 2007
Last modified: September 27, 2017
This is version 113 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families