Reviewed, UniProtKB/Swiss-Prot Q02589 (ADPRH_RAT)

Names and origin

Protein names

Recommended name:
    [Protein ADP-ribosylarginine] hydrolase
      Short name=ADP-ribosylarginine hydrolase
    EC=3.2.2.19
Alternative name(s):
    ADP-ribose-L-arginine cleaving enzyme

Gene names

Name:	Adprh
Synonyms:	Arh1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the reverse reaction of mono-ADP-ribosylation.
Catalytic activity	Protein-N(omega)-(ADP-D-ribosyl)-L-arginine + H2O = ADP-ribose + protein-L-arginine. N(omega)-(ADP-D-ribosyl)-L-arginine + H2O = ADP-ribose + L-arginine.
Enzyme regulation	Its activity is synergistically stimulated by magnesium and dithiothreitol (DTT) in vitro.
Sequence similarities	Belongs to the ADP-ribosylglycohydrolase family.

Ontologies

Keywords
Ligand	Magnesium
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	protein amino acid de-ADP-ribosylation Inferred from electronic annotation. Source: InterPro
Molecular function	ADP-ribosylarginine hydrolase activity Ref.1 Inferred from mutant phenotype. Source: UniProtKB magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	[Protein ADP-ribosylarginine] hydrolase		PRO_0000157285
Experimental info
	Sequence conflict	252	1	K → Q in AAA40691. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q02589-1 [UniParc]. Last modified May 29, 2007. Version 2. Checksum: 6C941A940AEAE10E	FASTA	362	39,961
	10 20 30 40 50 60 MGGGLIERYV AAMVLSAAGD TLGYFNGKWE FLRDGEKIHR QLAQMGDLEA IDVAQWRVSD 70 80 90 100 110 120 DTIMHLATAE ALMEAGSSPD LPQLYSLLAK HYRDCMGDMD GRAPGGACMQ NAMQLDPDRA 130 140 150 160 170 180 DGWRIPFNSH EGGCGAAMRA MCIGLRFPHP SQLDTLIQVS IESGRMTHHH PTGYLGSLAS 190 200 210 220 230 240 ALFTAYAVNG KSPRQWGKGL MEVLPEAKAY VTQSGYFVKE NLQHWSYFEK EWEKYLELRG 250 260 270 280 290 300 ILDGKSAPVF PKPFGVKERD QFYIEVSYSG WGGSSGHDAP MIAYDALLAA GDSWKELAHR 310 320 330 340 350 360 AFFHGGDSDS TATIAGCWWG VMHGFKGVNP SNYEKLEYRQ RLEEAGRALY SLGSKEDTIL GP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular and immunological characterization of ADP-ribosylarginine hydrolases." Moss J., Stanley S.J., Nightingale M.S., Murtagh J.J. Jr., Monaco L., Mishima K., Chen H.C., Williamson K.C., Tsai S.C. J. Biol. Chem. 267:10481-10488(1992) [PubMed: 1375222] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[3]	Lubec G., Afjehi-Sadat L., Chen W.-Q. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 147-165, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus and Spinal cord.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M86341 mRNA. Translation: AAA40691.1. BC082065 mRNA. Translation: AAH82065.1.
IPI	IPI00208197.
PIR	A38135.
RefSeq	NP_899154.2.
UniGene	Rn.13315
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000038439; ENSRNOP00000034815; ENSRNOG00000027260; Rattus norvegicus. [Genome view]
GeneID	25371.
KEGG	rno:25371.
NMPDR	fig|10116.3.peg.7179.
UCSC	NM_183325. rat.
Organism-specific databases
CTD	25371.
RGD	2052. Adprh.
Phylogenomic databases
HOVERGEN	Q02589.
OMA	IPFNSHE.
Enzyme and pathway databases
BRENDA	3.2.2.19. 248.
Gene expression databases
ArrayExpress	Q02589.
Genevestigator	Q02589.
GermOnline	ENSRNOG00000027260. Rattus norvegicus.
Family and domain databases
InterPro	IPR012108. ADP-ribosylarg_hydro. IPR005502. Ribosyl_crysJ1. [Graphical view]
PANTHER	PTHR22957:SF7. ADP-ribosylarg_hydro. 1 hit.
Pfam	PF03747. ADP_ribosyl_GH. 1 hit. [Graphical view]
PIRSF	PIRSF016939. ADP_ribslarg_hdr. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	606385.

Entry information

Entry name

ADPRH_RAT

Accession

Primary (citable) accession number: Q02589
Secondary accession number(s): Q66H27

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	May 29, 2007
Last modified:	November 3, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)