Reviewed, UniProtKB/Swiss-Prot Q02574 (MEC3_YEAST)

Names and origin

Protein names

Recommended name:
    DNA damage checkpoint control protein MEC3

Gene names

Name:	MEC3
Synonyms:	PIP3, PSO9
Ordered Locus Names:	YLR288C
ORF Names:	L8003.15

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the checkpoint clamp complex involved in the surveillance mechanism that allows the DNA repair pathways to act to restore the integrity of the DNA prior to DNA synthesis or separation of the replicated chromosomes. Associates with sites of DNA damage and modulates the MEC1 signaling pathway and the activation of RAD53 in response to DNA damage at phase G1. The complex also physically regulates DNA polymerase zeta-dependent mutagenesis by controlling the access of polymerase zeta to damaged DNA. Ref.5 Ref.6 Ref.7 Ref.8 Ref.10 Ref.12 Ref.13 Ref.14
Subunit structure	Component of the checkpoint clamp complex composed of DDC1, MEC3 and RAD17. The interaction with MEC3 is performed in a RAD17-dependent manner. The checkpoint clamp complex loads onto DNA. Interacts with the DNA polymerase zeta subunit REV7. Forms also an heterotrimer with 2 RAD17 subunits. Interacts with SET1. Ref.6 Ref.10 Ref.13 Ref.14 Ref.4 Ref.11
Subcellular location	Nucleus Potential.
Miscellaneous	Present with 414 molecules/cell in log phase SD medium. Ref.9
Sequence similarities	Belongs to the MEC3 family.

Ontologies

Keywords
Biological process	Cell cycle DNA damage DNA repair
Cellular component	Nucleus
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA damage checkpoint Inferred from mutant phenotype. Source: SGD DNA repair Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW chromatin silencing at telomere Ref.6 Inferred from mutant phenotype. Source: SGD telomere maintenance via recombination Inferred from genetic interaction. Source: SGD
Cellular component	checkpoint clamp complex Ref.4 Ref.5 Ref.10 Inferred from direct assay. Source: SGD
Molecular function	DNA binding Inferred from direct assay. Source: SGD protein binding Ref.4 Ref.6 Ref.13 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P34251	1	EBI-10658,EBI-26820
	P36092	1	EBI-10658,EBI-26659
	Q12138	1	EBI-10658,EBI-37507
ATG16	Q03818	3	EBI-10658,EBI-27344
DDC1	Q08949	1	EBI-10658,EBI-30769
ECM4	P36156	1	EBI-10658,EBI-2042717
RAD17	P48581	4	EBI-10658,EBI-14652
REV7	P38927	2	EBI-10658,EBI-14960
SAM50	P53969	1	EBI-10658,EBI-28646
SET1	P38827	2	EBI-10658,EBI-16977
TFB1	P32776	1	EBI-10658,EBI-19146

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 474	474	DNA damage checkpoint control protein MEC3		PRO_0000096344
Amino acid modifications
	Modified residue	50	1	Phosphoserine Ref.15
	Modified residue	452	1	Phosphoserine Ref.15 Ref.16
Experimental info
	Sequence conflict	120	1	T → I in AAB61448. Ref.1
	Sequence conflict	176	1	N → D in AAB61448. Ref.1
	Sequence conflict	454	1	D → E in AAB61448. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q02574-1 [UniParc]. Last modified June 13, 2006. Version 3. Checksum: 4385CEFA9130FEA3	FASTA	474	53,146
	10 20 30 40 50 60 MKLKLIVNGC EAPDDYKLLR TTINTVASLR KTAILRFNSE RLTIISTPKS SLNSSNNGTI 70 80 90 100 110 120 LRGDTGQLWC TIPHDVFRLY TVISARELNT ITMECNCDSL LSVFKRYDRV MNQGSSSNMT 130 140 150 160 170 180 IKLQSMPEWN TNNGTLSGGT AGGVDTTSKP NPICALGITF EEIVHTSGPN DAIVMNGGVD 190 200 210 220 230 240 EHNGLPTTVG TGNLLASNKV IMHSFKVPVK LLFRAQDTRI QEPMINYIQL MMYKLPPISG 250 260 270 280 290 300 EFGSAFHGFI RRVERYSNVN HIHLMGVKKK EHGNEGDDVE LKIIVNELDW HLEICWNGPL 310 320 330 340 350 360 DSVIQRQEGL TDNPSQNQHI DTDGRQEEGS LPIIEADKPM SSLYTNTRDR EMEENIRYDE 370 380 390 400 410 420 DLLRIEDSSI ADTRGNIYTA DTSGDTEFND ISVMVEKAEQ ESSSTHEVII RCKDWKVCSK 430 440 450 460 470 LYAAFEEVVL AISHDESCVF HCSLDRGSLE DSEDVEKPRE RGQIIYYIAR SKGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast checkpoint genes in DNA damage processing: implications for repair and arrest." Lydall D., Weinert T.A. Science 270:1488-1491(1995) [PubMed: 7491494] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Lydall D., Weinert T.A. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 27; 411; 415-222 AND 454.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	"Mec1p is essential for phosphorylation of the yeast DNA damage checkpoint protein Ddc1p, which physically interacts with Mec3p." Paciotti V., Lucchini G., Plevani P., Longhese M.P. EMBO J. 17:4199-4209(1998) [PubMed: 9670034] [Abstract] Cited for: INTERACTION WITH DDC1.
[5]	"Role of a complex containing Rad17, Mec3, and Ddc1 in the yeast DNA damage checkpoint pathway." Kondo T., Matsumoto K., Sugimoto K. Mol. Cell. Biol. 19:1136-1143(1999) [PubMed: 9891048] [Abstract] Cited for: IDENTIFICATION IN THE CHECKPOINT CLAMP COMPLEX, FUNCTION OF THE CHECKPOINT CLAMP COMPLEX.
[6]	"Interaction between Set1p and checkpoint protein Mec3p in DNA repair and telomere functions." Corda Y., Schramke V., Longhese M.P., Smokvina T., Paciotti V., Brevet V., Gilson E., Geli V. Nat. Genet. 21:204-208(1999) [PubMed: 9988274] [Abstract] Cited for: FUNCTION, INTERACTION WITH SET1.
[7]	"A dominant-negative MEC3 mutant uncovers new functions for the Rad17 complex and Tel1." Giannattasio M., Sommariva E., Vercillo R., Lippi-Boncambi F., Liberi G., Foiani M., Plevani P., Muzi-Falconi M. Proc. Natl. Acad. Sci. U.S.A. 99:12997-13002(2002) [PubMed: 12271137] [Abstract] Cited for: FUNCTION OF THE CHECKPOINT CLAMP COMPLEX.
[8]	"Correlation between checkpoint activation and in vivo assembly of the yeast checkpoint complex Rad17-Mec3-Ddc1." Giannattasio M., Sabbioneda S., Minuzzo M., Plevani P., Muzi-Falconi M. J. Biol. Chem. 278:22303-22308(2003) [PubMed: 12672803] [Abstract] Cited for: IDENTIFICATION IN THE CHECKPOINT CLAMP COMPLEX, FUNCTION OF THE CHECKPOINT CLAMP COMPLEX.
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"Yeast Rad17/Mec3/Ddc1: a sliding clamp for the DNA damage checkpoint." Majka J., Burgers P.M.J. Proc. Natl. Acad. Sci. U.S.A. 100:2249-2254(2003) [PubMed: 12604797] [Abstract] Cited for: INTERACTION OF THE CHECKPOINT CLAMP COMPLEX WITH THE RFC-RAD24 CHECKPOINT CLAMP LOADER COMPLEX, FUNCTION OF THE CHECKPOINT CLAMP COMPLEX.
[11]	"Requirement for ATP by the DNA damage checkpoint clamp loader." Majka J., Chung B.Y., Burgers P.M.J. J. Biol. Chem. 279:20921-20926(2004) [PubMed: 15014082] [Abstract] Cited for: INTERACTION OF THE CHECKPOINT CLAMP COMPLEX WITH THE RFC-RAD24 CHECKPOINT CLAMP LOADER COMPLEX.
[12]	"Function of Rad17/Mec3/Ddc1 and its partial complexes in the DNA damage checkpoint." Majka J., Burgers P.M.J. DNA Repair 4:1189-1194(2005) [PubMed: 16137930] [Abstract] Cited for: IDENTIFICATION IN THE CHECKPOINT CLAMP COMPLEX, FUNCTION OF THE CHECKPOINT CLAMP COMPLEX.
[13]	"The 9-1-1 checkpoint clamp physically interacts with polzeta and is partially required for spontaneous polzeta-dependent mutagenesis in Saccharomyces cerevisiae." Sabbioneda S., Minesinger B.K., Giannattasio M., Plevani P., Muzi-Falconi M., Jinks-Robertson S. J. Biol. Chem. 280:38657-38665(2005) [PubMed: 16169844] [Abstract] Cited for: INTERACTION WITH REV7, FUNCTION OF THE CHECKPOINT CLAMP COMPLEX.
[14]	"Psoralen-sensitive mutant pso9-1 of Saccharomyces cerevisiae contains a mutant allele of the DNA damage checkpoint gene MEC3." Cardone J.M., Revers L.F., Machado R.M., Bonatto D., Brendel M., Henriques J.A.P. DNA Repair 5:163-171(2006) [PubMed: 16202664] [Abstract] Cited for: FUNCTION, INTERACTION WITH DDC1 AND RAD17.
[15]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50 AND SER-452, MASS SPECTROMETRY.
[16]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-452, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U30906 Genomic DNA. Translation: AAB61448.1. U17243 Genomic DNA. Translation: AAB67334.1.
PIR	S50373.
RefSeq	NP_013391.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1432N.
IntAct	Q02574. 105 interactions.
STRING	Q02574.
Proteomic databases
PeptideAtlas	Q02574.
PRIDE	Q02574.
Genome annotation databases
Ensembl	YLR288C; YLR288C; YLR288C; Saccharomyces cerevisiae. [Genome view]
GeneID	850995.
KEGG	sce:YLR288C.
NMPDR	fig|4932.3.peg.4408.
Organism-specific databases
CYGD	YLR288c.
SGD	S000004279. MEC3.
Phylogenomic databases
HOGENOM	HBG202527.
OrthoDB	EOG94XM0D.
Gene expression databases
ArrayExpress	Q02574.
Genevestigator	Q02574.
GermOnline	YLR288C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	967528.

Entry information

Entry name

MEC3_YEAST

Accession

Primary (citable) accession number: Q02574
Secondary accession number(s): P87260, Q05887

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	June 13, 2006
Last modified:	December 15, 2009
This is version 71 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)