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Q02566

- MYH6_MOUSE

UniProt

Q02566 - MYH6_MOUSE

Protein

Myosin-6

Gene

Myh6

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 2 (15 Jul 1998)
      Previous versions | rss
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    Functioni

    Muscle contraction.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi178 – 1858ATP

    GO - Molecular functioni

    1. actin-dependent ATPase activity Source: Ensembl
    2. ATP binding Source: UniProtKB-KW
    3. microfilament motor activity Source: MGI
    4. protein binding Source: MGI
    5. structural constituent of muscle Source: Ensembl

    GO - Biological processi

    1. actin filament-based movement Source: MGI
    2. adult heart development Source: MGI
    3. atrial cardiac muscle tissue morphogenesis Source: Ensembl
    4. BMP signaling pathway Source: MGI
    5. canonical Wnt signaling pathway Source: MGI
    6. cardiac muscle fiber development Source: MGI
    7. in utero embryonic development Source: MGI
    8. muscle filament sliding Source: Ensembl
    9. myofibril assembly Source: MGI
    10. regulation of ATPase activity Source: MGI
    11. regulation of blood pressure Source: MGI
    12. regulation of heart contraction Source: MGI
    13. regulation of heart growth Source: MGI
    14. regulation of heart rate Source: MGI
    15. regulation of the force of heart contraction Source: MGI
    16. sarcomere organization Source: MGI
    17. striated muscle contraction Source: MGI
    18. ventricular cardiac muscle tissue morphogenesis Source: Ensembl
    19. visceral muscle development Source: MGI

    Keywords - Molecular functioni

    Motor protein, Muscle protein, Myosin

    Keywords - Ligandi

    Actin-binding, ATP-binding, Calmodulin-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myosin-6
    Alternative name(s):
    Myosin heavy chain 6
    Myosin heavy chain, cardiac muscle alpha isoform
    Short name:
    MyHC-alpha
    Gene namesi
    Name:Myh6
    Synonyms:Myhca
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:97255. Myh6.

    Subcellular locationi

    Cytoplasmmyofibril
    Note: Thick filaments of the myofibrils.

    GO - Cellular componenti

    1. focal adhesion Source: Ensembl
    2. myofibril Source: MGI
    3. myosin complex Source: MGI
    4. myosin filament Source: UniProtKB-KW
    5. nucleolus Source: Ensembl
    6. stress fiber Source: MGI
    7. Z disc Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Thick filament

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 19381938Myosin-6PRO_0000123403Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei129 – 1291N6,N6,N6-trimethyllysineSequence Analysis
    Cross-linki206 – 206Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
    Cross-linki207 – 207Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
    Cross-linki214 – 214Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
    Cross-linki1281 – 1281Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
    Cross-linki1539 – 1539Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
    Cross-linki1571 – 1571Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

    Keywords - PTMi

    Isopeptide bond, Methylation, Ubl conjugation

    Proteomic databases

    MaxQBiQ02566.
    PaxDbiQ02566.
    PRIDEiQ02566.

    2D gel databases

    SWISS-2DPAGEQ02566.

    PTM databases

    PhosphoSiteiQ02566.

    Expressioni

    Gene expression databases

    ArrayExpressiQ02566.
    BgeeiQ02566.
    GenevestigatoriQ02566.

    Interactioni

    Subunit structurei

    Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

    Protein-protein interaction databases

    BioGridi201651. 1 interaction.
    IntActiQ02566. 2 interactions.
    MINTiMINT-4102751.

    Structurei

    3D structure databases

    ProteinModelPortaliQ02566.
    SMRiQ02566. Positions 3-963.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini85 – 780696Myosin motorAdd
    BLAST
    Domaini783 – 81230IQPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni657 – 67923Actin-bindingAdd
    BLAST
    Regioni759 – 77315Actin-bindingAdd
    BLAST
    Regioni790 – 80718Calmodulin-bindingBy similarityAdd
    BLAST
    Regioni816 – 83318Calmodulin-bindingBy similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili842 – 19381097Sequence AnalysisAdd
    BLAST

    Domaini

    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

    Sequence similaritiesi

    Contains 1 IQ domain.PROSITE-ProRule annotation
    Contains 1 myosin motor domain.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG5022.
    GeneTreeiENSGT00720000108683.
    HOGENOMiHOG000173959.
    HOVERGENiHBG004704.
    InParanoidiQ02566.
    KOiK17751.
    OMAiISQQNSK.
    PhylomeDBiQ02566.
    TreeFamiTF314375.

    Family and domain databases

    Gene3Di4.10.270.10. 1 hit.
    InterProiIPR000048. IQ_motif_EF-hand-BS.
    IPR027401. Myosin-like_IQ_dom.
    IPR001609. Myosin_head_motor_dom.
    IPR004009. Myosin_N.
    IPR002928. Myosin_tail.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00063. Myosin_head. 1 hit.
    PF02736. Myosin_N. 1 hit.
    PF01576. Myosin_tail_1. 1 hit.
    [Graphical view]
    PRINTSiPR00193. MYOSINHEAVY.
    SMARTiSM00015. IQ. 2 hits.
    SM00242. MYSc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS50096. IQ. 1 hit.
    PS51456. MYOSIN_MOTOR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q02566-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDAQMADFG AAAQYLRKSE KERLEAQTRP FDIRTECFVP DDKEEYVKAK     50
    VVSREGGKVT AETENGKTVT IKEDQVMQQN PPKFDKIEDM AMLTFLHEPA 100
    VLYNLKERYA AWMIYTYSGL FCVTVNPYKW LPVYNAEVVA AYRGKKRSEA 150
    PPHIFSISDN AYQYMLTDRE NQSILITGES GAGKTVNTKR VIQYFASIAA 200
    IGDRSKKENP NANKGTLEDQ IIQANPALEA FGNAKTVRND NSSRFGKFIR 250
    IHFGATGKLA SADIETYLLE KSRVIFQLKA ERNYHIFYQI LSNKKPELLD 300
    MLLVTNNPYD YAFVSQGEVS VASIDDSEEL LATDSAFDVL SFTAEEKAGV 350
    YKLTGAIMHY GNMKFKQKQR EEQAEPDGTE DADKSAYLMG LNSADLLKGL 400
    CHPRVKVGNE YVTKGQSVQQ VYYSIGALAK SVYEKMFNWM VTRINATLET 450
    KQPRQYFIGV LDIAGFEIFD FNSFEQLCIN FTNEKLQQFF NHHMFVLEQE 500
    EYKKEGIEWE FIDFGMDLQA CIDLIEKPMG IMSILEEECM FPKASDMTFK 550
    AKLYDNHLGK SNNFQKPRNV KGKQEAHFSL VHYAGTVDYN IMGWLEKNKD 600
    PLNETVVGLY QKSSLKLMAT LFSTYASADT GDSGKGKGGK KKGSSFQTVS 650
    ALHRENLNKL MTNLKTTHPH FVRCIIPNER KAPGVMDNPL VMHQLRCNGV 700
    LEGIRICRKG FPNRILYGDF RQRYRILNPA AIPEGQFIDS RKGAEKLLGS 750
    LDIDHNQYKF GHTKVFFKAG LLGLLEEMRD ERLSRIITRI QAQARGQLMR 800
    IEFKKIVERR DALLVIQWNI RAFMGVKNWP WMKLYFKIKP LLKSAETEKE 850
    MANMKEEFGR VKDALEKSEA RRKELEEKMV SLLQEKNDLQ LQVQAEQDNL 900
    NDAEERCDQL IKNKIQLEAK VKEMTERLED EEEMNAELTA KKRKLEDECS 950
    ELKKDIDDLE LTLAKVEKEK HATENKVKNL TEEMAGLDEI IAKLTKEKKA 1000
    LQEAHQQALD DLQAEEDKVN TLTKSKVKLE QQVDDLEGSL EQEKKVRMDL 1050
    ERAKRKLEGD LKLTQESIMD LENDKLQLEE KLKKKEFDIS QQNSKIEDEQ 1100
    ALALQLQKKL KENQARIEEL EEELEAERTA RAKVEKLRSD LSRELEEISE 1150
    RLEEAGGATS VQIEMNKKRE AEFQKMRRDL EEATLQHEAT AAALRKKHAD 1200
    SVAELGEQID NLQRVKQKLE KEKSEFKLEL DDVTSNMEQI IKAKANLEKV 1250
    SRTLEDQANE YRVKLEEAQR SLNDFTTQRA KLQTENGELA RQLEEKEALI 1300
    SQLTRGKLSY TQQMEDLKRQ LEEEGKAKNA LAHALQSSRH DCDLLREQYE 1350
    EEMEAKAELQ RVLSKANSEV AQWRTKYETD AIQRTEELEE AKKKLAQRLQ 1400
    DAEEAVEAVN AKCSSLEKTK HRLQNEIEDL MVDVERSNAA AAALDKKQRN 1450
    FDKILAEWKQ KYEESQSELE SSQKEARSLS TELFKLKNAY EESLEHLETF 1500
    KRENKNLQEE ISDLTEQLGE GGKNVHELEK IRKQLEVEKL ELQSALEEAE 1550
    ASLEHEEGKI LRAQLEFNQI KAEIERKLAE KDEEMEQAKR NHLRMVDSLQ 1600
    TSLDAETRSR NEALRVKKKM EGDLNEMEIQ LSQANRIASE AQKHLKNSQA 1650
    HLKDTQLQLD DAVHANDDLK ENIAIVERRN NLLQAELEEL RAVVEQTERS 1700
    RKLAEQELIE TSERVQLLHS QNTSLINQKK KMESDLTQLQ TEVEEAVQEC 1750
    RNAEEKAKKA ITDAAMMAEE LKKEQDTSAH LERMKKNMEQ TIKDLQHRLD 1800
    EAEQIALKGG KKQLQKLEAR VRELENELEA EQKRNAESVK GMRKSERRIK 1850
    ELTYQTEEDK KNLMRLQDLV DKLQLKVKAY KRQAEEAEEQ ANTNLSKFRK 1900
    VQHELDEAEE RADIAESQVN KLRAKSRDIG AKKMHDEE 1938
    Length:1,938
    Mass (Da):223,565
    Last modified:July 15, 1998 - v2
    Checksum:iEAD789ADA68818FB
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti194 – 1941Y → D.
    Natural varianti545 – 5451S → A.
    Natural varianti838 – 8381I → S.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M76598 mRNA. Translation: AAA37159.1.
    M76599 mRNA. Translation: AAA37160.1.
    M76600 mRNA. Translation: AAA37161.1.
    M76601 mRNA. Translation: AAA37162.1.
    M62404 Genomic DNA. Translation: AAA37424.1.
    CCDSiCCDS36927.1.
    PIRiI49464.
    RefSeqiNP_001157643.1. NM_001164171.1.
    NP_034986.1. NM_010856.4.
    UniGeneiMm.290003.

    Genome annotation databases

    EnsembliENSMUST00000081857; ENSMUSP00000080538; ENSMUSG00000040752.
    GeneIDi17888.
    KEGGimmu:17888.
    UCSCiuc007txr.2. mouse.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M76598 mRNA. Translation: AAA37159.1 .
    M76599 mRNA. Translation: AAA37160.1 .
    M76600 mRNA. Translation: AAA37161.1 .
    M76601 mRNA. Translation: AAA37162.1 .
    M62404 Genomic DNA. Translation: AAA37424.1 .
    CCDSi CCDS36927.1.
    PIRi I49464.
    RefSeqi NP_001157643.1. NM_001164171.1.
    NP_034986.1. NM_010856.4.
    UniGenei Mm.290003.

    3D structure databases

    ProteinModelPortali Q02566.
    SMRi Q02566. Positions 3-963.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201651. 1 interaction.
    IntActi Q02566. 2 interactions.
    MINTi MINT-4102751.

    PTM databases

    PhosphoSitei Q02566.

    2D gel databases

    SWISS-2DPAGE Q02566.

    Proteomic databases

    MaxQBi Q02566.
    PaxDbi Q02566.
    PRIDEi Q02566.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000081857 ; ENSMUSP00000080538 ; ENSMUSG00000040752 .
    GeneIDi 17888.
    KEGGi mmu:17888.
    UCSCi uc007txr.2. mouse.

    Organism-specific databases

    CTDi 4624.
    MGIi MGI:97255. Myh6.

    Phylogenomic databases

    eggNOGi COG5022.
    GeneTreei ENSGT00720000108683.
    HOGENOMi HOG000173959.
    HOVERGENi HBG004704.
    InParanoidi Q02566.
    KOi K17751.
    OMAi ISQQNSK.
    PhylomeDBi Q02566.
    TreeFami TF314375.

    Miscellaneous databases

    ChiTaRSi MYH6. mouse.
    NextBioi 292705.
    PROi Q02566.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q02566.
    Bgeei Q02566.
    Genevestigatori Q02566.

    Family and domain databases

    Gene3Di 4.10.270.10. 1 hit.
    InterProi IPR000048. IQ_motif_EF-hand-BS.
    IPR027401. Myosin-like_IQ_dom.
    IPR001609. Myosin_head_motor_dom.
    IPR004009. Myosin_N.
    IPR002928. Myosin_tail.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00063. Myosin_head. 1 hit.
    PF02736. Myosin_N. 1 hit.
    PF01576. Myosin_tail_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00193. MYOSINHEAVY.
    SMARTi SM00015. IQ. 2 hits.
    SM00242. MYSc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS50096. IQ. 1 hit.
    PS51456. MYOSIN_MOTOR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the allelic differences in the mouse cardiac alpha-myosin heavy chain coding sequence."
      Quinn-Laquer B.K., Kennedy J.E., Wei S.J., Beisel K.W.
      Genomics 13:176-188(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: A/J, BALB/c, C57BL/6J and DBA/2J.
    2. "Isolation and characterization of the mouse cardiac myosin heavy chain genes."
      Gulick J., Subramaniam A., Neumann J., Robbins J.
      J. Biol. Chem. 266:9180-9185(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-67.
      Strain: AKR.
    3. "A proteomics approach to identify the ubiquitinated proteins in mouse heart."
      Jeon H.B., Choi E.S., Yoon J.H., Hwang J.H., Chang J.W., Lee E.K., Choi H.W., Park Z.-Y., Yoo Y.J.
      Biochem. Biophys. Res. Commun. 357:731-736(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-206; LYS-207; LYS-214; LYS-1281; LYS-1539 AND LYS-1571.
      Tissue: Heart.

    Entry informationi

    Entry nameiMYH6_MOUSE
    AccessioniPrimary (citable) accession number: Q02566
    Secondary accession number(s): Q64258, Q64738
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: July 15, 1998
    Last modified: October 1, 2014
    This is version 124 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The cardiac alpha isoform is a 'fast' ATPase myosin, while the beta isoform is a 'slow' ATPase.

    Caution

    Represents a conventional myosin. This protein should not be confused with the unconventional myosin-6 (MYO6).Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3