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Protein

Myosin-6

Gene

Myh6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Muscle contraction.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi178 – 1858ATP

GO - Molecular functioni

  1. actin-dependent ATPase activity Source: MGI
  2. ATPase activity Source: MGI
  3. ATP binding Source: UniProtKB-KW
  4. microfilament motor activity Source: MGI
  5. protein kinase binding Source: MGI

GO - Biological processi

  1. actin filament-based movement Source: MGI
  2. adult heart development Source: MGI
  3. atrial cardiac muscle tissue morphogenesis Source: MGI
  4. BMP signaling pathway Source: MGI
  5. canonical Wnt signaling pathway Source: MGI
  6. cardiac muscle contraction Source: BHF-UCL
  7. cardiac muscle fiber development Source: MGI
  8. in utero embryonic development Source: MGI
  9. metabolic process Source: MGI
  10. muscle contraction Source: MGI
  11. muscle filament sliding Source: MGI
  12. myofibril assembly Source: MGI
  13. regulation of ATPase activity Source: MGI
  14. regulation of blood pressure Source: MGI
  15. regulation of heart contraction Source: MGI
  16. regulation of heart growth Source: MGI
  17. regulation of heart rate Source: MGI
  18. regulation of the force of heart contraction Source: MGI
  19. sarcomere organization Source: MGI
  20. striated muscle contraction Source: MGI
  21. ventricular cardiac muscle tissue morphogenesis Source: MGI
  22. visceral muscle development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Muscle protein, Myosin

Keywords - Ligandi

Actin-binding, ATP-binding, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_324616. Striated Muscle Contraction.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin-6
Alternative name(s):
Myosin heavy chain 6
Myosin heavy chain, cardiac muscle alpha isoform
Short name:
MyHC-alpha
Gene namesi
Name:Myh6
Synonyms:Myhca
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:97255. Myh6.

Subcellular locationi

  1. Cytoplasmmyofibril

  2. Note: Thick filaments of the myofibrils.

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. focal adhesion Source: MGI
  3. myofibril Source: MGI
  4. myosin complex Source: MGI
  5. myosin filament Source: UniProtKB-KW
  6. nucleoplasm Source: MGI
  7. stress fiber Source: MGI
  8. Z disc Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Thick filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 19381938Myosin-6PRO_0000123403Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei129 – 1291N6,N6,N6-trimethyllysineSequence Analysis
Cross-linki206 – 206Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki207 – 207Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki214 – 214Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki1281 – 1281Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki1539 – 1539Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki1571 – 1571Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Keywords - PTMi

Isopeptide bond, Methylation, Ubl conjugation

Proteomic databases

MaxQBiQ02566.
PaxDbiQ02566.
PRIDEiQ02566.

2D gel databases

SWISS-2DPAGEQ02566.

PTM databases

PhosphoSiteiQ02566.

Expressioni

Gene expression databases

BgeeiQ02566.
ExpressionAtlasiQ02566. baseline and differential.
GenevestigatoriQ02566.

Interactioni

Subunit structurei

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

Protein-protein interaction databases

BioGridi201651. 1 interaction.
DIPiDIP-31385N.
IntActiQ02566. 2 interactions.
MINTiMINT-4102751.

Structurei

3D structure databases

ProteinModelPortaliQ02566.
SMRiQ02566. Positions 3-963.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini85 – 780696Myosin motorAdd
BLAST
Domaini783 – 81230IQPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni657 – 67923Actin-bindingAdd
BLAST
Regioni759 – 77315Actin-bindingAdd
BLAST
Regioni790 – 80718Calmodulin-bindingBy similarityAdd
BLAST
Regioni816 – 83318Calmodulin-bindingBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili842 – 19381097Sequence AnalysisAdd
BLAST

Domaini

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

Sequence similaritiesi

Contains 1 IQ domain.PROSITE-ProRule annotation
Contains 1 myosin motor domain.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5022.
GeneTreeiENSGT00760000118919.
HOGENOMiHOG000173959.
HOVERGENiHBG004704.
InParanoidiQ02566.
KOiK17751.
OMAiQANEYRM.
PhylomeDBiQ02566.
TreeFamiTF314375.

Family and domain databases

Gene3Di4.10.270.10. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00015. IQ. 2 hits.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q02566-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDAQMADFG AAAQYLRKSE KERLEAQTRP FDIRTECFVP DDKEEYVKAK
60 70 80 90 100
VVSREGGKVT AETENGKTVT IKEDQVMQQN PPKFDKIEDM AMLTFLHEPA
110 120 130 140 150
VLYNLKERYA AWMIYTYSGL FCVTVNPYKW LPVYNAEVVA AYRGKKRSEA
160 170 180 190 200
PPHIFSISDN AYQYMLTDRE NQSILITGES GAGKTVNTKR VIQYFASIAA
210 220 230 240 250
IGDRSKKENP NANKGTLEDQ IIQANPALEA FGNAKTVRND NSSRFGKFIR
260 270 280 290 300
IHFGATGKLA SADIETYLLE KSRVIFQLKA ERNYHIFYQI LSNKKPELLD
310 320 330 340 350
MLLVTNNPYD YAFVSQGEVS VASIDDSEEL LATDSAFDVL SFTAEEKAGV
360 370 380 390 400
YKLTGAIMHY GNMKFKQKQR EEQAEPDGTE DADKSAYLMG LNSADLLKGL
410 420 430 440 450
CHPRVKVGNE YVTKGQSVQQ VYYSIGALAK SVYEKMFNWM VTRINATLET
460 470 480 490 500
KQPRQYFIGV LDIAGFEIFD FNSFEQLCIN FTNEKLQQFF NHHMFVLEQE
510 520 530 540 550
EYKKEGIEWE FIDFGMDLQA CIDLIEKPMG IMSILEEECM FPKASDMTFK
560 570 580 590 600
AKLYDNHLGK SNNFQKPRNV KGKQEAHFSL VHYAGTVDYN IMGWLEKNKD
610 620 630 640 650
PLNETVVGLY QKSSLKLMAT LFSTYASADT GDSGKGKGGK KKGSSFQTVS
660 670 680 690 700
ALHRENLNKL MTNLKTTHPH FVRCIIPNER KAPGVMDNPL VMHQLRCNGV
710 720 730 740 750
LEGIRICRKG FPNRILYGDF RQRYRILNPA AIPEGQFIDS RKGAEKLLGS
760 770 780 790 800
LDIDHNQYKF GHTKVFFKAG LLGLLEEMRD ERLSRIITRI QAQARGQLMR
810 820 830 840 850
IEFKKIVERR DALLVIQWNI RAFMGVKNWP WMKLYFKIKP LLKSAETEKE
860 870 880 890 900
MANMKEEFGR VKDALEKSEA RRKELEEKMV SLLQEKNDLQ LQVQAEQDNL
910 920 930 940 950
NDAEERCDQL IKNKIQLEAK VKEMTERLED EEEMNAELTA KKRKLEDECS
960 970 980 990 1000
ELKKDIDDLE LTLAKVEKEK HATENKVKNL TEEMAGLDEI IAKLTKEKKA
1010 1020 1030 1040 1050
LQEAHQQALD DLQAEEDKVN TLTKSKVKLE QQVDDLEGSL EQEKKVRMDL
1060 1070 1080 1090 1100
ERAKRKLEGD LKLTQESIMD LENDKLQLEE KLKKKEFDIS QQNSKIEDEQ
1110 1120 1130 1140 1150
ALALQLQKKL KENQARIEEL EEELEAERTA RAKVEKLRSD LSRELEEISE
1160 1170 1180 1190 1200
RLEEAGGATS VQIEMNKKRE AEFQKMRRDL EEATLQHEAT AAALRKKHAD
1210 1220 1230 1240 1250
SVAELGEQID NLQRVKQKLE KEKSEFKLEL DDVTSNMEQI IKAKANLEKV
1260 1270 1280 1290 1300
SRTLEDQANE YRVKLEEAQR SLNDFTTQRA KLQTENGELA RQLEEKEALI
1310 1320 1330 1340 1350
SQLTRGKLSY TQQMEDLKRQ LEEEGKAKNA LAHALQSSRH DCDLLREQYE
1360 1370 1380 1390 1400
EEMEAKAELQ RVLSKANSEV AQWRTKYETD AIQRTEELEE AKKKLAQRLQ
1410 1420 1430 1440 1450
DAEEAVEAVN AKCSSLEKTK HRLQNEIEDL MVDVERSNAA AAALDKKQRN
1460 1470 1480 1490 1500
FDKILAEWKQ KYEESQSELE SSQKEARSLS TELFKLKNAY EESLEHLETF
1510 1520 1530 1540 1550
KRENKNLQEE ISDLTEQLGE GGKNVHELEK IRKQLEVEKL ELQSALEEAE
1560 1570 1580 1590 1600
ASLEHEEGKI LRAQLEFNQI KAEIERKLAE KDEEMEQAKR NHLRMVDSLQ
1610 1620 1630 1640 1650
TSLDAETRSR NEALRVKKKM EGDLNEMEIQ LSQANRIASE AQKHLKNSQA
1660 1670 1680 1690 1700
HLKDTQLQLD DAVHANDDLK ENIAIVERRN NLLQAELEEL RAVVEQTERS
1710 1720 1730 1740 1750
RKLAEQELIE TSERVQLLHS QNTSLINQKK KMESDLTQLQ TEVEEAVQEC
1760 1770 1780 1790 1800
RNAEEKAKKA ITDAAMMAEE LKKEQDTSAH LERMKKNMEQ TIKDLQHRLD
1810 1820 1830 1840 1850
EAEQIALKGG KKQLQKLEAR VRELENELEA EQKRNAESVK GMRKSERRIK
1860 1870 1880 1890 1900
ELTYQTEEDK KNLMRLQDLV DKLQLKVKAY KRQAEEAEEQ ANTNLSKFRK
1910 1920 1930
VQHELDEAEE RADIAESQVN KLRAKSRDIG AKKMHDEE
Length:1,938
Mass (Da):223,565
Last modified:July 15, 1998 - v2
Checksum:iEAD789ADA68818FB
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti194 – 1941Y → D.
Natural varianti545 – 5451S → A.
Natural varianti838 – 8381I → S.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76598 mRNA. Translation: AAA37159.1.
M76599 mRNA. Translation: AAA37160.1.
M76600 mRNA. Translation: AAA37161.1.
M76601 mRNA. Translation: AAA37162.1.
M62404 Genomic DNA. Translation: AAA37424.1.
CCDSiCCDS36927.1.
PIRiI49464.
RefSeqiNP_001157643.1. NM_001164171.1.
NP_034986.1. NM_010856.4.
UniGeneiMm.290003.

Genome annotation databases

EnsembliENSMUST00000081857; ENSMUSP00000080538; ENSMUSG00000040752.
GeneIDi17888.
KEGGimmu:17888.
UCSCiuc007txr.2. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76598 mRNA. Translation: AAA37159.1.
M76599 mRNA. Translation: AAA37160.1.
M76600 mRNA. Translation: AAA37161.1.
M76601 mRNA. Translation: AAA37162.1.
M62404 Genomic DNA. Translation: AAA37424.1.
CCDSiCCDS36927.1.
PIRiI49464.
RefSeqiNP_001157643.1. NM_001164171.1.
NP_034986.1. NM_010856.4.
UniGeneiMm.290003.

3D structure databases

ProteinModelPortaliQ02566.
SMRiQ02566. Positions 3-963.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201651. 1 interaction.
DIPiDIP-31385N.
IntActiQ02566. 2 interactions.
MINTiMINT-4102751.

PTM databases

PhosphoSiteiQ02566.

2D gel databases

SWISS-2DPAGEQ02566.

Proteomic databases

MaxQBiQ02566.
PaxDbiQ02566.
PRIDEiQ02566.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000081857; ENSMUSP00000080538; ENSMUSG00000040752.
GeneIDi17888.
KEGGimmu:17888.
UCSCiuc007txr.2. mouse.

Organism-specific databases

CTDi4624.
MGIiMGI:97255. Myh6.

Phylogenomic databases

eggNOGiCOG5022.
GeneTreeiENSGT00760000118919.
HOGENOMiHOG000173959.
HOVERGENiHBG004704.
InParanoidiQ02566.
KOiK17751.
OMAiQANEYRM.
PhylomeDBiQ02566.
TreeFamiTF314375.

Enzyme and pathway databases

ReactomeiREACT_324616. Striated Muscle Contraction.

Miscellaneous databases

ChiTaRSiMyh6. mouse.
NextBioi292705.
PROiQ02566.
SOURCEiSearch...

Gene expression databases

BgeeiQ02566.
ExpressionAtlasiQ02566. baseline and differential.
GenevestigatoriQ02566.

Family and domain databases

Gene3Di4.10.270.10. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00015. IQ. 2 hits.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the allelic differences in the mouse cardiac alpha-myosin heavy chain coding sequence."
    Quinn-Laquer B.K., Kennedy J.E., Wei S.J., Beisel K.W.
    Genomics 13:176-188(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: A/J, BALB/c, C57BL/6J and DBA/2J.
  2. "Isolation and characterization of the mouse cardiac myosin heavy chain genes."
    Gulick J., Subramaniam A., Neumann J., Robbins J.
    J. Biol. Chem. 266:9180-9185(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-67.
    Strain: AKR.
  3. "A proteomics approach to identify the ubiquitinated proteins in mouse heart."
    Jeon H.B., Choi E.S., Yoon J.H., Hwang J.H., Chang J.W., Lee E.K., Choi H.W., Park Z.-Y., Yoo Y.J.
    Biochem. Biophys. Res. Commun. 357:731-736(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-206; LYS-207; LYS-214; LYS-1281; LYS-1539 AND LYS-1571.
    Tissue: Heart.

Entry informationi

Entry nameiMYH6_MOUSE
AccessioniPrimary (citable) accession number: Q02566
Secondary accession number(s): Q64258, Q64738
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: April 29, 2015
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The cardiac alpha isoform is a 'fast' ATPase myosin, while the beta isoform is a 'slow' ATPase.

Caution

Represents a conventional myosin. This protein should not be confused with the unconventional myosin-6 (MYO6).Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.