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Q02566 (MYH6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Myosin-6
Alternative name(s):
Myosin heavy chain 6
Myosin heavy chain, cardiac muscle alpha isoform
Short name=MyHC-alpha
Gene names
Name:Myh6
Synonyms:Myhca
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length1938 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Muscle contraction.

Subunit structure

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

Subcellular location

Cytoplasm › myofibril. Note: Thick filaments of the myofibrils.

Domain

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.

Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

Miscellaneous

The cardiac alpha isoform is a 'fast' ATPase myosin, while the beta isoform is a 'slow' ATPase.

Sequence similarities

Contains 1 IQ domain.

Contains 1 myosin head-like domain.

Caution

Represents a conventional myosin. This protein should not be confused with the unconventional myosin-6 (MYO6).

Ontologies

Keywords
   Cellular componentCytoplasm
Thick filament
   Coding sequence diversityPolymorphism
   DomainCoiled coil
   LigandATP-binding
Actin-binding
Calmodulin-binding
Nucleotide-binding
   Molecular functionMotor protein
Muscle protein
Myosin
   PTMIsopeptide bond
Methylation
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processATP catabolic process

Inferred from electronic annotation. Source: GOC

BMP signaling pathway

Inferred from direct assay PubMed 17360443. Source: MGI

actin filament-based movement

Inferred from mutant phenotype PubMed 15001446. Source: MGI

adult heart development

Inferred from mutant phenotype PubMed 10066683PubMed 15001446PubMed 16983074PubMed 8878443PubMed 9884344. Source: MGI

atrial cardiac muscle tissue morphogenesis

Inferred from electronic annotation. Source: Compara

canonical Wnt receptor signaling pathway

Inferred from direct assay PubMed 17360443. Source: MGI

cardiac muscle fiber development

Inferred from mutant phenotype PubMed 10066683PubMed 15001446PubMed 8878443PubMed 9884344. Source: MGI

in utero embryonic development

Inferred from mutant phenotype PubMed 8878443. Source: MGI

muscle filament sliding

Inferred from electronic annotation. Source: Compara

regulation of ATPase activity

Inferred from mutant phenotype PubMed 15001446. Source: MGI

regulation of blood pressure

Inferred from mutant phenotype PubMed 8878443. Source: MGI

regulation of heart growth

Inferred from direct assay PubMed 17379774. Source: MGI

regulation of heart rate

Inferred from mutant phenotype PubMed 9541509. Source: MGI

regulation of the force of heart contraction

Inferred from mutant phenotype PubMed 9541509. Source: MGI

sarcomere organization

Inferred from mutant phenotype PubMed 8878443. Source: MGI

striated muscle contraction

Inferred from mutant phenotype PubMed 10066683PubMed 10074482PubMed 15001446PubMed 16983074PubMed 8614836PubMed 8878443PubMed 9045856PubMed 9541509PubMed 9884344. Source: MGI

ventricular cardiac muscle tissue morphogenesis

Inferred from electronic annotation. Source: Compara

visceral muscle development

Inferred from mutant phenotype PubMed 11708849. Source: MGI

   Cellular_componentZ disc

Inferred from direct assay PubMed 21126233. Source: MGI

focal adhesion

Inferred from electronic annotation. Source: Compara

myosin complex

Inferred from direct assay PubMed 15001446PubMed 8482409PubMed 9884344. Source: MGI

myosin filament

Inferred from electronic annotation. Source: UniProtKB-KW

nucleolus

Inferred from electronic annotation. Source: Compara

stress fiber

Inferred from direct assay PubMed 21126233. Source: MGI

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

actin-dependent ATPase activity

Inferred from electronic annotation. Source: Compara

microfilament motor activity

Inferred from mutant phenotype PubMed 15001446PubMed 16983074. Source: MGI

structural constituent of muscle

Inferred from electronic annotation. Source: Compara

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 19381938Myosin-6
PRO_0000123403

Regions

Domain1 – 782782Myosin head-like
Domain783 – 81230IQ
Nucleotide binding178 – 1858ATP
Region657 – 67923Actin-binding
Region759 – 77315Actin-binding
Region790 – 80718Calmodulin-binding By similarity
Region816 – 83318Calmodulin-binding By similarity
Coiled coil842 – 19381097 Potential

Amino acid modifications

Modified residue1291N6,N6,N6-trimethyllysine Potential
Cross-link206Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.3
Cross-link207Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.3
Cross-link214Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.3
Cross-link1281Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.3
Cross-link1539Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.3
Cross-link1571Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.3

Natural variations

Natural variant1941Y → D.
Natural variant5451S → A.
Natural variant8381I → S.

Sequences

Sequence LengthMass (Da)Tools
Q02566 [UniParc].

Last modified July 15, 1998. Version 2.
Checksum: EAD789ADA68818FB

FASTA1,938223,565
        10         20         30         40         50         60 
MTDAQMADFG AAAQYLRKSE KERLEAQTRP FDIRTECFVP DDKEEYVKAK VVSREGGKVT 

        70         80         90        100        110        120 
AETENGKTVT IKEDQVMQQN PPKFDKIEDM AMLTFLHEPA VLYNLKERYA AWMIYTYSGL 

       130        140        150        160        170        180 
FCVTVNPYKW LPVYNAEVVA AYRGKKRSEA PPHIFSISDN AYQYMLTDRE NQSILITGES 

       190        200        210        220        230        240 
GAGKTVNTKR VIQYFASIAA IGDRSKKENP NANKGTLEDQ IIQANPALEA FGNAKTVRND 

       250        260        270        280        290        300 
NSSRFGKFIR IHFGATGKLA SADIETYLLE KSRVIFQLKA ERNYHIFYQI LSNKKPELLD 

       310        320        330        340        350        360 
MLLVTNNPYD YAFVSQGEVS VASIDDSEEL LATDSAFDVL SFTAEEKAGV YKLTGAIMHY 

       370        380        390        400        410        420 
GNMKFKQKQR EEQAEPDGTE DADKSAYLMG LNSADLLKGL CHPRVKVGNE YVTKGQSVQQ 

       430        440        450        460        470        480 
VYYSIGALAK SVYEKMFNWM VTRINATLET KQPRQYFIGV LDIAGFEIFD FNSFEQLCIN 

       490        500        510        520        530        540 
FTNEKLQQFF NHHMFVLEQE EYKKEGIEWE FIDFGMDLQA CIDLIEKPMG IMSILEEECM 

       550        560        570        580        590        600 
FPKASDMTFK AKLYDNHLGK SNNFQKPRNV KGKQEAHFSL VHYAGTVDYN IMGWLEKNKD 

       610        620        630        640        650        660 
PLNETVVGLY QKSSLKLMAT LFSTYASADT GDSGKGKGGK KKGSSFQTVS ALHRENLNKL 

       670        680        690        700        710        720 
MTNLKTTHPH FVRCIIPNER KAPGVMDNPL VMHQLRCNGV LEGIRICRKG FPNRILYGDF 

       730        740        750        760        770        780 
RQRYRILNPA AIPEGQFIDS RKGAEKLLGS LDIDHNQYKF GHTKVFFKAG LLGLLEEMRD 

       790        800        810        820        830        840 
ERLSRIITRI QAQARGQLMR IEFKKIVERR DALLVIQWNI RAFMGVKNWP WMKLYFKIKP 

       850        860        870        880        890        900 
LLKSAETEKE MANMKEEFGR VKDALEKSEA RRKELEEKMV SLLQEKNDLQ LQVQAEQDNL 

       910        920        930        940        950        960 
NDAEERCDQL IKNKIQLEAK VKEMTERLED EEEMNAELTA KKRKLEDECS ELKKDIDDLE 

       970        980        990       1000       1010       1020 
LTLAKVEKEK HATENKVKNL TEEMAGLDEI IAKLTKEKKA LQEAHQQALD DLQAEEDKVN 

      1030       1040       1050       1060       1070       1080 
TLTKSKVKLE QQVDDLEGSL EQEKKVRMDL ERAKRKLEGD LKLTQESIMD LENDKLQLEE 

      1090       1100       1110       1120       1130       1140 
KLKKKEFDIS QQNSKIEDEQ ALALQLQKKL KENQARIEEL EEELEAERTA RAKVEKLRSD 

      1150       1160       1170       1180       1190       1200 
LSRELEEISE RLEEAGGATS VQIEMNKKRE AEFQKMRRDL EEATLQHEAT AAALRKKHAD 

      1210       1220       1230       1240       1250       1260 
SVAELGEQID NLQRVKQKLE KEKSEFKLEL DDVTSNMEQI IKAKANLEKV SRTLEDQANE 

      1270       1280       1290       1300       1310       1320 
YRVKLEEAQR SLNDFTTQRA KLQTENGELA RQLEEKEALI SQLTRGKLSY TQQMEDLKRQ 

      1330       1340       1350       1360       1370       1380 
LEEEGKAKNA LAHALQSSRH DCDLLREQYE EEMEAKAELQ RVLSKANSEV AQWRTKYETD 

      1390       1400       1410       1420       1430       1440 
AIQRTEELEE AKKKLAQRLQ DAEEAVEAVN AKCSSLEKTK HRLQNEIEDL MVDVERSNAA 

      1450       1460       1470       1480       1490       1500 
AAALDKKQRN FDKILAEWKQ KYEESQSELE SSQKEARSLS TELFKLKNAY EESLEHLETF 

      1510       1520       1530       1540       1550       1560 
KRENKNLQEE ISDLTEQLGE GGKNVHELEK IRKQLEVEKL ELQSALEEAE ASLEHEEGKI 

      1570       1580       1590       1600       1610       1620 
LRAQLEFNQI KAEIERKLAE KDEEMEQAKR NHLRMVDSLQ TSLDAETRSR NEALRVKKKM 

      1630       1640       1650       1660       1670       1680 
EGDLNEMEIQ LSQANRIASE AQKHLKNSQA HLKDTQLQLD DAVHANDDLK ENIAIVERRN 

      1690       1700       1710       1720       1730       1740 
NLLQAELEEL RAVVEQTERS RKLAEQELIE TSERVQLLHS QNTSLINQKK KMESDLTQLQ 

      1750       1760       1770       1780       1790       1800 
TEVEEAVQEC RNAEEKAKKA ITDAAMMAEE LKKEQDTSAH LERMKKNMEQ TIKDLQHRLD 

      1810       1820       1830       1840       1850       1860 
EAEQIALKGG KKQLQKLEAR VRELENELEA EQKRNAESVK GMRKSERRIK ELTYQTEEDK 

      1870       1880       1890       1900       1910       1920 
KNLMRLQDLV DKLQLKVKAY KRQAEEAEEQ ANTNLSKFRK VQHELDEAEE RADIAESQVN 

      1930 
KLRAKSRDIG AKKMHDEE

« Hide

References

« Hide 'large scale' references
[1]"Characterization of the allelic differences in the mouse cardiac alpha-myosin heavy chain coding sequence."
Quinn-Laquer B.K., Kennedy J.E., Wei S.J., Beisel K.W.
Genomics 13:176-188(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: A/J, BALB/c, C57BL/6J and DBA/2J.
[2]"Isolation and characterization of the mouse cardiac myosin heavy chain genes."
Gulick J., Subramaniam A., Neumann J., Robbins J.
J. Biol. Chem. 266:9180-9185(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-67.
Strain: AKR.
[3]"A proteomics approach to identify the ubiquitinated proteins in mouse heart."
Jeon H.B., Choi E.S., Yoon J.H., Hwang J.H., Chang J.W., Lee E.K., Choi H.W., Park Z.-Y., Yoo Y.J.
Biochem. Biophys. Res. Commun. 357:731-736(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-206; LYS-207; LYS-214; LYS-1281; LYS-1539 AND LYS-1571, MASS SPECTROMETRY.
Tissue: Heart.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M76598 mRNA. Translation: AAA37159.1.
M76599 mRNA. Translation: AAA37160.1.
M76600 mRNA. Translation: AAA37161.1.
M76601 mRNA. Translation: AAA37162.1.
M62404 Genomic DNA. Translation: AAA37424.1.
IPIIPI00129404.
PIRI49464.
RefSeqNP_001157643.1. NM_001164171.1.
NP_034986.1. NM_010856.4.
UniGeneMm.290003.

3D structure databases

ProteinModelPortalQ02566.
SMRQ02566. Positions 3-963.
ModBaseSearch...

Protein-protein interaction databases

IntActQ02566. 1 interaction.

PTM databases

PhosphoSiteQ02566.

2D gel databases

SWISS-2DPAGEQ02566.

Proteomic databases

PaxDbQ02566.
PRIDEQ02566.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000081857; ENSMUSP00000080538; ENSMUSG00000040752.
GeneID17888.
KEGGmmu:17888.

Organism-specific databases

CTD4624.
MGIMGI:97255. Myh6.

Phylogenomic databases

eggNOGCOG5022.
GeneTreeENSGT00680000099788.
HOGENOMHOG000173959.
HOVERGENHBG004704.
InParanoidQ02566.
KOK10352.
OMAISQQNSK.

Gene expression databases

ArrayExpressQ02566.
BgeeQ02566.
GenevestigatorQ02566.
GermOnlineENSMUSG00000040752. Mus musculus.

Family and domain databases

InterProIPR000048. IQ_motif_EF-hand-BS.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
[Graphical view]
PfamPF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view]
PRINTSPR00193. MYOSINHEAVY.
SMARTSM00015. IQ. 2 hits.
SM00242. MYSc. 1 hit.
[Graphical view]
PROSITEPS50096. IQ. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMYH6. mouse.
NextBio292705.
SOURCESearch...

Entry information

Entry nameMYH6_MOUSE
AccessionPrimary (citable) accession number: Q02566
Secondary accession number(s): Q64258, Q64738
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: May 1, 2013
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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