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Q02555

- RNT1_YEAST

UniProt

Q02555 - RNT1_YEAST

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Protein

Ribonuclease 3

Gene

RNT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

DsRNA-specific nuclease that cleaves eukaryotic pre-ribosomal RNA at the U3 snoRNP-dependent A0 site in the 5'-external transcribed spacer (ETS) and in the 3'-ETS. In vitro, cleaves synthetic 5'-ETS RNA A0 site in the absence of snoRNA or other factors. Has an essential growth function in addition to pre-rRNA processing.

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

GO - Molecular functioni

  1. ribonuclease III activity Source: SGD
  2. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. box C/D snoRNA processing Source: SGD
  2. box H/ACA snoRNA processing Source: SGD
  3. chromatin organization Source: SGD
  4. mRNA cleavage Source: SGD
  5. regulation of fungal-type cell wall organization Source: SGD
  6. RNA phosphodiester bond hydrolysis Source: GOC
  7. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
  8. rRNA catabolic process Source: InterPro
  9. rRNA processing Source: SGD
  10. rRNA transcription Source: SGD
  11. termination of RNA polymerase II transcription, exosome-dependent Source: SGD
  12. U1 snRNA 3'-end processing Source: SGD
  13. U4 snRNA 3'-end processing Source: SGD
  14. U5 snRNA 3'-end processing Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:YMR239C-MONOMER.
BRENDAi3.1.26.3. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 3 (EC:3.1.26.3)
Alternative name(s):
Ribonuclease III
Short name:
RNase III
Gene namesi
Name:RNT1
Ordered Locus Names:YMR239C
ORF Names:YM9408.01C, YM9959.21
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR239c.
SGDiS000004852. RNT1.

Subcellular locationi

GO - Cellular componenti

  1. nucleolus Source: SGD
  2. nucleoplasm Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 471471Ribonuclease 3PRO_0000180466Add
BLAST

Proteomic databases

MaxQBiQ02555.
PaxDbiQ02555.

Expressioni

Gene expression databases

GenevestigatoriQ02555.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SEN1Q004162EBI-15673,EBI-16945

Protein-protein interaction databases

BioGridi35418. 50 interactions.
DIPiDIP-4298N.
IntActiQ02555. 8 interactions.
MINTiMINT-567072.

Structurei

Secondary structure

1
471
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi46 – 6924Combined sources
Helixi73 – 819Combined sources
Beta strandi84 – 863Combined sources
Helixi88 – 958Combined sources
Helixi97 – 11014Combined sources
Helixi116 – 12611Combined sources
Beta strandi136 – 1383Combined sources
Helixi140 – 14910Combined sources
Helixi208 – 2136Combined sources
Helixi218 – 2214Combined sources
Helixi229 – 2335Combined sources
Helixi238 – 25922Combined sources
Helixi265 – 27612Combined sources
Helixi278 – 28710Combined sources
Helixi290 – 2934Combined sources
Turni306 – 3094Combined sources
Helixi313 – 32917Combined sources
Helixi331 – 35626Combined sources
Helixi358 – 3603Combined sources
Helixi370 – 3789Combined sources
Helixi381 – 3833Combined sources
Beta strandi386 – 3916Combined sources
Beta strandi394 – 3963Combined sources
Beta strandi400 – 4056Combined sources
Turni407 – 4093Combined sources
Beta strandi411 – 4199Combined sources
Helixi420 – 43314Combined sources
Helixi435 – 4417Combined sources
Helixi450 – 4534Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T4LNMR-B366-453[»]
1T4NNMR-A364-447[»]
1T4OX-ray2.50A/B362-471[»]
2LBSNMR-B366-453[»]
2LUPNMR-B366-453[»]
2LUQNMR-A366-453[»]
4OOGX-ray2.50A/B42-151[»]
C197-457[»]
ProteinModelPortaliQ02555.
SMRiQ02555. Positions 42-151, 197-457.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ02555.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini227 – 331105RNase IIIPROSITE-ProRule annotationAdd
BLAST
Domaini369 – 43769DRBMPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DRBM (double-stranded RNA-binding) domain.PROSITE-ProRule annotation
Contains 1 RNase III domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0571.
HOGENOMiHOG000000766.
InParanoidiQ02555.
KOiK03685.
OMAiYAALNLH.
OrthoDBiEOG7M0P26.

Family and domain databases

Gene3Di1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
InterProiIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERiPTHR11207. PTHR11207. 1 hit.
PfamiPF00035. dsrm. 1 hit.
PF00636. Ribonuclease_3. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMiSSF69065. SSF69065. 1 hit.
PROSITEiPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q02555-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGSKVAGKKK TQNDNKLDNE NGSQQRENIN TKTLLKGNLK ISNYKYLEVI
60 70 80 90 100
QLEHAVTKLV ESYNKIIELS PNLVAYNEAV NNQDRVPVQI LPSLSRYQLK
110 120 130 140 150
LAAELKTLHD LKKDAILTEI TDYENEFDTE QKQPILQEIS KADMEKLEKL
160 170 180 190 200
EQVKREKREK IDVNVYENLN EKEDEEEDEG EDSYDPTKAG DIVKATKWPP
210 220 230 240 250
KLPEIQDLAI RARVFIHKST IKDKVYLSGS EMINAHNERL EFLGDSILNS
260 270 280 290 300
VMTLIIYNKF PDYSEGQLST LRMNLVSNEQ IKQWSIMYNF HEKLKTNFDL
310 320 330 340 350
KDENSNFQNG KLKLYADVFE AYIGGLMEDD PRNNLPKIRK WLRKLAKPVI
360 370 380 390 400
EEATRNQVAL EKTDKLDMNA KRQLYSLIGY ASLRLHYVTV KKPTAVDPNS
410 420 430 440 450
IVECRVGDGT VLGTGVGRNI KIAGIRAAEN ALRDKKMLDF YAKQRAAIPR
460 470
SESVLKDPSQ KNKKRKFSDT S
Length:471
Mass (Da):54,071
Last modified:November 1, 1997 - v1
Checksum:i570294172E66A0DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27016 Genomic DNA. Translation: AAB04172.1.
Z48756 Genomic DNA. Translation: CAA88649.1.
Z49939 Genomic DNA. Translation: CAA90210.1.
BK006946 Genomic DNA. Translation: DAA10139.1.
PIRiS56053.
RefSeqiNP_013966.1. NM_001182746.1.

Genome annotation databases

EnsemblFungiiYMR239C; YMR239C; YMR239C.
GeneIDi855280.
KEGGisce:YMR239C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27016 Genomic DNA. Translation: AAB04172.1 .
Z48756 Genomic DNA. Translation: CAA88649.1 .
Z49939 Genomic DNA. Translation: CAA90210.1 .
BK006946 Genomic DNA. Translation: DAA10139.1 .
PIRi S56053.
RefSeqi NP_013966.1. NM_001182746.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1T4L NMR - B 366-453 [» ]
1T4N NMR - A 364-447 [» ]
1T4O X-ray 2.50 A/B 362-471 [» ]
2LBS NMR - B 366-453 [» ]
2LUP NMR - B 366-453 [» ]
2LUQ NMR - A 366-453 [» ]
4OOG X-ray 2.50 A/B 42-151 [» ]
C 197-457 [» ]
ProteinModelPortali Q02555.
SMRi Q02555. Positions 42-151, 197-457.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35418. 50 interactions.
DIPi DIP-4298N.
IntActi Q02555. 8 interactions.
MINTi MINT-567072.

Proteomic databases

MaxQBi Q02555.
PaxDbi Q02555.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR239C ; YMR239C ; YMR239C .
GeneIDi 855280.
KEGGi sce:YMR239C.

Organism-specific databases

CYGDi YMR239c.
SGDi S000004852. RNT1.

Phylogenomic databases

eggNOGi COG0571.
HOGENOMi HOG000000766.
InParanoidi Q02555.
KOi K03685.
OMAi YAALNLH.
OrthoDBi EOG7M0P26.

Enzyme and pathway databases

BioCyci YEAST:YMR239C-MONOMER.
BRENDAi 3.1.26.3. 984.

Miscellaneous databases

EvolutionaryTracei Q02555.
NextBioi 978911.
PROi Q02555.

Gene expression databases

Genevestigatori Q02555.

Family and domain databases

Gene3Di 1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
InterProi IPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view ]
PANTHERi PTHR11207. PTHR11207. 1 hit.
Pfami PF00035. dsrm. 1 hit.
PF00636. Ribonuclease_3. 1 hit.
[Graphical view ]
SMARTi SM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view ]
SUPFAMi SSF69065. SSF69065. 1 hit.
PROSITEi PS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "RNase III cleaves eukaryotic preribosomal RNA at a U3 snoRNP-dependent site."
    Elela S.A., Igel H., Ares M. Jr.
    Cell 85:115-124(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRNT1_YEAST
AccessioniPrimary (citable) accession number: Q02555
Secondary accession number(s): D6W065, Q04008, Q05038
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 26, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4970 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3