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Q02555 (RNT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease 3

EC=3.1.26.3
Alternative name(s):
Ribonuclease III
Short name=RNase III
Gene names
Name:RNT1
Ordered Locus Names:YMR239C
ORF Names:YM9408.01C, YM9959.21
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length471 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DsRNA-specific nuclease that cleaves eukaryotic pre-ribosomal RNA at the U3 snoRNP-dependent A0 site in the 5'-external transcribed spacer (ETS) and in the 3'-ETS. In vitro, cleaves synthetic 5'-ETS RNA A0 site in the absence of snoRNA or other factors. Has an essential growth function in addition to pre-rRNA processing.

Catalytic activity

Endonucleolytic cleavage to 5'-phosphomonoester.

Miscellaneous

Present with 4970 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 DRBM (double-stranded RNA-binding) domain.

Contains 1 RNase III domain.

Ontologies

Keywords
   LigandRNA-binding
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRNA phosphodiester bond hydrolysis

Inferred from direct assay PubMed 15766245. Source: GOC

RNA phosphodiester bond hydrolysis, endonucleolytic

Inferred from direct assay PubMed 15766245. Source: GOC

U1 snRNA 3'-end processing

Inferred from mutant phenotype PubMed 10508172. Source: SGD

U4 snRNA 3'-end processing

Inferred from mutant phenotype PubMed 10508172. Source: SGD

U5 snRNA 3'-end processing

Inferred from mutant phenotype PubMed 10508172. Source: SGD

box C/D snoRNA processing

Inferred from mutant phenotype PubMed 23201338. Source: SGD

box H/ACA snoRNA processing

Inferred from mutant phenotype PubMed 23201338. Source: SGD

chromatin organization

Inferred from mutant phenotype PubMed 17991894. Source: SGD

mRNA cleavage

Inferred from direct assay PubMed 17158880. Source: SGD

rRNA catabolic process

Inferred from electronic annotation. Source: InterPro

rRNA processing

Inferred from mutant phenotype PubMed 17991894. Source: SGD

rRNA transcription

Inferred from mutant phenotype PubMed 17991894. Source: SGD

regulation of fungal-type cell wall organization

Inferred from mutant phenotype PubMed 22576366. Source: SGD

termination of RNA polymerase II transcription, exosome-dependent

Inferred from direct assay PubMed 19818713. Source: SGD

   Cellular_componentnucleolus

Inferred from direct assay PubMed 15090619. Source: SGD

nucleoplasm

Inferred from direct assay PubMed 15090619. Source: SGD

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

ribonuclease III activity

Inferred from direct assay PubMed 15766245. Source: SGD

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SEN1Q004162EBI-15673,EBI-16945

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 471471Ribonuclease 3
PRO_0000180466

Regions

Domain227 – 331105RNase III
Domain369 – 43769DRBM

Secondary structure

.................. 471
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q02555 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 570294172E66A0DE

FASTA47154,071
        10         20         30         40         50         60 
MGSKVAGKKK TQNDNKLDNE NGSQQRENIN TKTLLKGNLK ISNYKYLEVI QLEHAVTKLV 

        70         80         90        100        110        120 
ESYNKIIELS PNLVAYNEAV NNQDRVPVQI LPSLSRYQLK LAAELKTLHD LKKDAILTEI 

       130        140        150        160        170        180 
TDYENEFDTE QKQPILQEIS KADMEKLEKL EQVKREKREK IDVNVYENLN EKEDEEEDEG 

       190        200        210        220        230        240 
EDSYDPTKAG DIVKATKWPP KLPEIQDLAI RARVFIHKST IKDKVYLSGS EMINAHNERL 

       250        260        270        280        290        300 
EFLGDSILNS VMTLIIYNKF PDYSEGQLST LRMNLVSNEQ IKQWSIMYNF HEKLKTNFDL 

       310        320        330        340        350        360 
KDENSNFQNG KLKLYADVFE AYIGGLMEDD PRNNLPKIRK WLRKLAKPVI EEATRNQVAL 

       370        380        390        400        410        420 
EKTDKLDMNA KRQLYSLIGY ASLRLHYVTV KKPTAVDPNS IVECRVGDGT VLGTGVGRNI 

       430        440        450        460        470 
KIAGIRAAEN ALRDKKMLDF YAKQRAAIPR SESVLKDPSQ KNKKRKFSDT S 

« Hide

References

« Hide 'large scale' references
[1]"RNase III cleaves eukaryotic preribosomal RNA at a U3 snoRNP-dependent site."
Elela S.A., Igel H., Ares M. Jr.
Cell 85:115-124(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U27016 Genomic DNA. Translation: AAB04172.1.
Z48756 Genomic DNA. Translation: CAA88649.1.
Z49939 Genomic DNA. Translation: CAA90210.1.
BK006946 Genomic DNA. Translation: DAA10139.1.
PIRS56053.
RefSeqNP_013966.1. NM_001182746.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1T4LNMR-B366-453[»]
1T4NNMR-A364-447[»]
1T4OX-ray2.50A/B362-471[»]
2LBSNMR-B366-453[»]
2LUPNMR-B366-453[»]
2LUQNMR-A366-453[»]
ProteinModelPortalQ02555.
SMRQ02555. Positions 94-453.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35418. 50 interactions.
DIPDIP-4298N.
IntActQ02555. 8 interactions.
MINTMINT-567072.

Proteomic databases

PaxDbQ02555.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR239C; YMR239C; YMR239C.
GeneID855280.
KEGGsce:YMR239C.

Organism-specific databases

CYGDYMR239c.
SGDS000004852. RNT1.

Phylogenomic databases

eggNOGCOG0571.
HOGENOMHOG000000766.
KOK03685.
OMAVIRARVF.
OrthoDBEOG7M0P26.

Enzyme and pathway databases

BioCycYEAST:YMR239C-MONOMER.
BRENDA3.1.26.3. 984.

Gene expression databases

GenevestigatorQ02555.

Family and domain databases

Gene3D1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
InterProIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERPTHR11207. PTHR11207. 1 hit.
PfamPF00035. dsrm. 1 hit.
PF00636. Ribonuclease_3. 1 hit.
[Graphical view]
SMARTSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMSSF69065. SSF69065. 1 hit.
PROSITEPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ02555.
NextBio978911.
PROQ02555.

Entry information

Entry nameRNT1_YEAST
AccessionPrimary (citable) accession number: Q02555
Secondary accession number(s): D6W065, Q04008, Q05038
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references