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Q02555

- RNT1_YEAST

UniProt

Q02555 - RNT1_YEAST

Protein

Ribonuclease 3

Gene

RNT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    DsRNA-specific nuclease that cleaves eukaryotic pre-ribosomal RNA at the U3 snoRNP-dependent A0 site in the 5'-external transcribed spacer (ETS) and in the 3'-ETS. In vitro, cleaves synthetic 5'-ETS RNA A0 site in the absence of snoRNA or other factors. Has an essential growth function in addition to pre-rRNA processing.

    Catalytic activityi

    Endonucleolytic cleavage to 5'-phosphomonoester.

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. ribonuclease III activity Source: SGD
    3. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. box C/D snoRNA processing Source: SGD
    2. box H/ACA snoRNA processing Source: SGD
    3. chromatin organization Source: SGD
    4. mRNA cleavage Source: SGD
    5. regulation of fungal-type cell wall organization Source: SGD
    6. RNA phosphodiester bond hydrolysis Source: GOC
    7. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
    8. rRNA catabolic process Source: InterPro
    9. rRNA processing Source: SGD
    10. rRNA transcription Source: SGD
    11. termination of RNA polymerase II transcription, exosome-dependent Source: SGD
    12. U1 snRNA 3'-end processing Source: SGD
    13. U4 snRNA 3'-end processing Source: SGD
    14. U5 snRNA 3'-end processing Source: SGD

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:YMR239C-MONOMER.
    BRENDAi3.1.26.3. 984.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease 3 (EC:3.1.26.3)
    Alternative name(s):
    Ribonuclease III
    Short name:
    RNase III
    Gene namesi
    Name:RNT1
    Ordered Locus Names:YMR239C
    ORF Names:YM9408.01C, YM9959.21
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR239c.
    SGDiS000004852. RNT1.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleolus Source: SGD
    2. nucleoplasm Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 471471Ribonuclease 3PRO_0000180466Add
    BLAST

    Proteomic databases

    MaxQBiQ02555.
    PaxDbiQ02555.

    Expressioni

    Gene expression databases

    GenevestigatoriQ02555.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SEN1Q004162EBI-15673,EBI-16945

    Protein-protein interaction databases

    BioGridi35418. 50 interactions.
    DIPiDIP-4298N.
    IntActiQ02555. 8 interactions.
    MINTiMINT-567072.

    Structurei

    Secondary structure

    1
    471
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi46 – 6924
    Helixi73 – 819
    Beta strandi84 – 863
    Helixi88 – 958
    Helixi97 – 11014
    Helixi116 – 12611
    Beta strandi136 – 1383
    Helixi140 – 14910
    Helixi208 – 2136
    Helixi218 – 2214
    Helixi229 – 2335
    Helixi238 – 25922
    Helixi265 – 27612
    Helixi278 – 28710
    Helixi290 – 2934
    Turni306 – 3094
    Helixi313 – 32917
    Helixi331 – 35626
    Helixi358 – 3603
    Helixi370 – 3789
    Helixi381 – 3833
    Beta strandi386 – 3916
    Beta strandi394 – 3963
    Beta strandi400 – 4056
    Turni407 – 4093
    Beta strandi411 – 4199
    Helixi420 – 43314
    Helixi435 – 4417
    Helixi450 – 4534

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1T4LNMR-B366-453[»]
    1T4NNMR-A364-447[»]
    1T4OX-ray2.50A/B362-471[»]
    2LBSNMR-B366-453[»]
    2LUPNMR-B366-453[»]
    2LUQNMR-A366-453[»]
    4OOGX-ray2.50A/B42-151[»]
    C197-457[»]
    ProteinModelPortaliQ02555.
    SMRiQ02555. Positions 201-453.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ02555.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini227 – 331105RNase IIIPROSITE-ProRule annotationAdd
    BLAST
    Domaini369 – 43769DRBMPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 DRBM (double-stranded RNA-binding) domain.PROSITE-ProRule annotation
    Contains 1 RNase III domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0571.
    HOGENOMiHOG000000766.
    KOiK03685.
    OMAiYAALNLH.
    OrthoDBiEOG7M0P26.

    Family and domain databases

    Gene3Di1.10.1520.10. 1 hit.
    3.30.160.20. 1 hit.
    InterProiIPR014720. dsRNA-bd_dom.
    IPR011907. RNase_III.
    IPR000999. RNase_III_dom.
    [Graphical view]
    PANTHERiPTHR11207. PTHR11207. 1 hit.
    PfamiPF00035. dsrm. 1 hit.
    PF00636. Ribonuclease_3. 1 hit.
    [Graphical view]
    SMARTiSM00358. DSRM. 1 hit.
    SM00535. RIBOc. 1 hit.
    [Graphical view]
    SUPFAMiSSF69065. SSF69065. 1 hit.
    PROSITEiPS50137. DS_RBD. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q02555-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSKVAGKKK TQNDNKLDNE NGSQQRENIN TKTLLKGNLK ISNYKYLEVI    50
    QLEHAVTKLV ESYNKIIELS PNLVAYNEAV NNQDRVPVQI LPSLSRYQLK 100
    LAAELKTLHD LKKDAILTEI TDYENEFDTE QKQPILQEIS KADMEKLEKL 150
    EQVKREKREK IDVNVYENLN EKEDEEEDEG EDSYDPTKAG DIVKATKWPP 200
    KLPEIQDLAI RARVFIHKST IKDKVYLSGS EMINAHNERL EFLGDSILNS 250
    VMTLIIYNKF PDYSEGQLST LRMNLVSNEQ IKQWSIMYNF HEKLKTNFDL 300
    KDENSNFQNG KLKLYADVFE AYIGGLMEDD PRNNLPKIRK WLRKLAKPVI 350
    EEATRNQVAL EKTDKLDMNA KRQLYSLIGY ASLRLHYVTV KKPTAVDPNS 400
    IVECRVGDGT VLGTGVGRNI KIAGIRAAEN ALRDKKMLDF YAKQRAAIPR 450
    SESVLKDPSQ KNKKRKFSDT S 471
    Length:471
    Mass (Da):54,071
    Last modified:November 1, 1997 - v1
    Checksum:i570294172E66A0DE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U27016 Genomic DNA. Translation: AAB04172.1.
    Z48756 Genomic DNA. Translation: CAA88649.1.
    Z49939 Genomic DNA. Translation: CAA90210.1.
    BK006946 Genomic DNA. Translation: DAA10139.1.
    PIRiS56053.
    RefSeqiNP_013966.1. NM_001182746.1.

    Genome annotation databases

    EnsemblFungiiYMR239C; YMR239C; YMR239C.
    GeneIDi855280.
    KEGGisce:YMR239C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U27016 Genomic DNA. Translation: AAB04172.1 .
    Z48756 Genomic DNA. Translation: CAA88649.1 .
    Z49939 Genomic DNA. Translation: CAA90210.1 .
    BK006946 Genomic DNA. Translation: DAA10139.1 .
    PIRi S56053.
    RefSeqi NP_013966.1. NM_001182746.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1T4L NMR - B 366-453 [» ]
    1T4N NMR - A 364-447 [» ]
    1T4O X-ray 2.50 A/B 362-471 [» ]
    2LBS NMR - B 366-453 [» ]
    2LUP NMR - B 366-453 [» ]
    2LUQ NMR - A 366-453 [» ]
    4OOG X-ray 2.50 A/B 42-151 [» ]
    C 197-457 [» ]
    ProteinModelPortali Q02555.
    SMRi Q02555. Positions 201-453.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35418. 50 interactions.
    DIPi DIP-4298N.
    IntActi Q02555. 8 interactions.
    MINTi MINT-567072.

    Proteomic databases

    MaxQBi Q02555.
    PaxDbi Q02555.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR239C ; YMR239C ; YMR239C .
    GeneIDi 855280.
    KEGGi sce:YMR239C.

    Organism-specific databases

    CYGDi YMR239c.
    SGDi S000004852. RNT1.

    Phylogenomic databases

    eggNOGi COG0571.
    HOGENOMi HOG000000766.
    KOi K03685.
    OMAi YAALNLH.
    OrthoDBi EOG7M0P26.

    Enzyme and pathway databases

    BioCyci YEAST:YMR239C-MONOMER.
    BRENDAi 3.1.26.3. 984.

    Miscellaneous databases

    EvolutionaryTracei Q02555.
    NextBioi 978911.
    PROi Q02555.

    Gene expression databases

    Genevestigatori Q02555.

    Family and domain databases

    Gene3Di 1.10.1520.10. 1 hit.
    3.30.160.20. 1 hit.
    InterProi IPR014720. dsRNA-bd_dom.
    IPR011907. RNase_III.
    IPR000999. RNase_III_dom.
    [Graphical view ]
    PANTHERi PTHR11207. PTHR11207. 1 hit.
    Pfami PF00035. dsrm. 1 hit.
    PF00636. Ribonuclease_3. 1 hit.
    [Graphical view ]
    SMARTi SM00358. DSRM. 1 hit.
    SM00535. RIBOc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69065. SSF69065. 1 hit.
    PROSITEi PS50137. DS_RBD. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "RNase III cleaves eukaryotic preribosomal RNA at a U3 snoRNP-dependent site."
      Elela S.A., Igel H., Ares M. Jr.
      Cell 85:115-124(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRNT1_YEAST
    AccessioniPrimary (citable) accession number: Q02555
    Secondary accession number(s): D6W065, Q04008, Q05038
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 4970 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3