Q02554 (CUS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cold sensitive U2 snRNA suppressor 1

Gene names

Name:	CUS1
Ordered Locus Names:	YMR240C
ORF Names:	YM9408.02C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential splicing protein required for U2 snRNP binding to pre-mRNA during spliceosome assembly.
Subunit structure	Interacts with RDS3. Ref.5
Subcellular location	Nucleus Potential.
Miscellaneous	Present with 1830 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	To mammalian SAP 145. Some, to C.elegans ZK632.11.

Ontologies

Keywords
Biological process	mRNA processing
Cellular component	Nucleus
Ligand	RNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	spliceosome assembly Inferred from direct assay Ref.1. Source: SGD
Cellular component	U2 snRNP Inferred from direct assay. Source: SGD U2-type prespliceosome Inferred from direct assay. Source: SGD
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
HSH49	Q99181	5	EBI-654,EBI-8579

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 436	436	Cold sensitive U2 snRNA suppressor 1		PRO_0000079565
Natural variations
	Natural variant	181	1	E → K in allele CUS1-54; cold-sensitive growth suppressor.

Sequences

	Sequence		Length	Mass (Da)
	Q02554 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 0204B3A666AF7336	FASTA	436	50,253
	10 20 30 40 50 60 MARTKSRKRS GNNQNKNASV VNNKAEIAAM IDARRLEQKK KGGVTNSKGK TNKVVDAKLE 70 80 90 100 110 120 KEFKDVLQRF QVQENDTPKE ITKDEKNNHV VIVEKNPVMN RKHTAEDELE DTPSDGIEEH 130 140 150 160 170 180 LSARKRRKTE KPSLSQLKSQ VPYPQIIEWY DCDARYPGLL ASIKCTKNVI PVPSHWQSKK 190 200 210 220 230 240 EYLSGRSLLG KRPFELPDII KKTNIEQMRS TLPQSGLDGQ DEKSLKEASR ARVQPKMGAL 250 260 270 280 290 300 DLDYKKLHDV FFKIGANWKP DHLLCFGDVY YENRNLFEET NWKRMVDHKR PGRISQELRA 310 320 330 340 350 360 IMNLPEGQLP PWCMKMKDIG LPTGYPDLKI AGLNWDITNL KGDVYGKIIP NHHSRSKKQG 370 380 390 400 410 420 RNYFGALISF ETPEFENSKE DTQANAENGR QDDKIDDEVE HKLDHFQEDI SEVTSAEEKL 430 ERNEEESEKQ LYTVLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CUS1, a suppressor of cold-sensitive U2 snRNA mutations, is a novel yeast splicing factor homologous to human SAP 145." Wells S.E., Neville M., Haynes M., Wang J., Igel H., Ares M. Jr. Genes Dev. 10:220-232(1996) [PubMed: 8566755] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Rds3p is required for stable U2 snRNP recruitment to the splicing apparatus." Wang Q., Rymond B.C. Mol. Cell. Biol. 23:7339-7349(2003) [PubMed: 14517302] [Abstract] Cited for: INTERACTION WITH RDS3.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U27016 Genomic DNA. Translation: AAB04171.1. Z48756 Genomic DNA. Translation: CAA88650.1. AY723856 Genomic DNA. Translation: AAU09773.1. BK006946 Genomic DNA. Translation: DAA10140.1.
PIR	S56054.
RefSeq	NP_013967.1. NM_001182747.1.
3D structure databases
ProteinModelPortal	Q02554.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-910N.
IntAct	Q02554. 30 interactions.
MINT	MINT-474095.
STRING	Q02554.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR240C; YMR240C; YMR240C.
GeneID	855281.
KEGG	sce:YMR240C.
NMPDR	fig|4932.3.peg.5021.
Organism-specific databases
CYGD	YMR240c.
SGD	S000004853. CUS1.
Phylogenomic databases
eggNOG	fuNOG06550.
GeneTree	EFGT00050000003796.
HOGENOM	HBG203500.
OMA	VYYENRN.
OrthoDB	EOG4VMJPZ.
Gene expression databases
ArrayExpress	Q02554.
Genevestigator	Q02554.
GermOnline	YMR240C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007180. DUF382. IPR006568. PSP. [Graphical view]
KO	K12829.
Pfam	PF04037. DUF382. 1 hit. PF04046. PSP. 1 hit. [Graphical view]
SMART	SM00581. PSP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978914.

Entry information

Entry name

CUS1_YEAST

Accession

Primary (citable) accession number: Q02554
Secondary accession number(s): D6W066, Q04012

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program