Reviewed, UniProtKB/Swiss-Prot Q02541 (COPS_PSESM)

Names and origin

Protein names

Recommended name:
    Sensor protein copS
    EC=2.7.13.3

Gene names

Name:

copS

Encoded on

Plasmid pPT23D

Organism

Pseudomonas syringae pv. tomato

Taxonomic identifier

323 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Member of the two-component regulatory system copS/copR. Involved in the activation of copper resistance gene operon copABCD. Specifically recognizes or transduces a signal only in response to copper. This would lead to phosphorylation of copR in the cytoplasm. CopS/copR may also regulate chromosomally encoded genes. May also be involved in basic copper metabolism.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Potential.
Sequence similarities	Contains 1 HAMP domain. Contains 1 histidine kinase domain.

Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Copper Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Plasmid
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro sensory perception Inferred from electronic annotation. Source: InterPro two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 487	487	Sensor protein copS		PRO_0000074737
Regions
	Topological domain	1 – 9	9	Periplasmic Potential
	Transmembrane	10 – 30	21	Potential
	Topological domain	31 – 136	106	Cytoplasmic Potential
	Transmembrane	137 – 157	21	Potential
	Topological domain	158 – 159	2	Periplasmic Potential
	Transmembrane	160 – 180	21	Potential
	Topological domain	181 – 487	307	Cytoplasmic Potential
	Domain	181 – 234	54	HAMP
	Domain	242 – 455	214	Histidine kinase
Amino acid modifications
	Modified residue	245	1	Phosphohistidine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q02541-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: A9D678AACBC077FD	FASTA	487	53,457
	10 20 30 40 50 60 MKPGSLTLRL SLLFVVAVAA VLIIVGVAFN ELSRHHFRAL DAQALGEKLE AITQIAKESG 70 80 90 100 110 120 ANPELLKARW HTLLGAHPDL SAVFLKTDGT PFFAEPPQSA VPSLAQATQR DGVWEWEKEG 130 140 150 160 170 180 RMFRALTASV SLPTASPPLT AWLVLDVTTH MHFFAMLERW FWGVLLASTV LSAALGWLVA 190 200 210 220 230 240 KNGLRPVARV TQTAASMSAG SLKERIPLEP VPDELRALIT AFNSMLGRLD DSFMRLSNFS 250 260 270 280 290 300 ADIAHELRTP ISNLRTHTEV ILAKKRAPEV YEENLSSNLE ELNRLSGIID GMLFLAKSDN 310 320 330 340 350 360 GLIVPEAVEL DLRTVISKLF GYYEFLAEDK GIQLQASGNA SIFADSVMID RVVSNLLSNA 370 380 390 400 410 420 LRYTSSGETI KVSIHDHGGR VELRLENPGP EIVPQHLDRI FDRFYRVDPA RREGRECGAG 430 440 450 460 470 480 ASDCPVLDAS AWRHYLVYIP RGPNDLHPHL HAIACPTNLT CRPDSLGTAK PGHTRLGEHE TGCHCAG

References

[1]	"A two-component regulatory system required for copper-inducible expression of the copper resistance operon of Pseudomonas syringae." Mills S.D., Jasalavich C.A., Cooksey D.A. J. Bacteriol. 175:1656-1664(1993) [PubMed: 8449873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L05176 Genomic DNA. Translation: AAA25804.1.
PIR	C47080.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR003594. ATP_bd_ATPase. IPR003660. HAMP_linker_domain. IPR006290. Sig_transdc_His_kin_metal-sen. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF00672. HAMP. 1 hit. PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. [Graphical view]
SMART	SM00304. HAMP. 1 hit. SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01386. cztS_silS_copS. 1 hit.
PROSITE	PS50885. HAMP. 1 hit. PS50109. HIS_KIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COPS_PSESM

Accession

Primary (citable) accession number: Q02541

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	October 13, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)