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Q02535

- ID3_HUMAN

UniProt

Q02535 - ID3_HUMAN

Protein

DNA-binding protein inhibitor ID-3

Gene

ID3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Involved in myogenesis by inhibiting skeletal muscle and cardiac myocyte differentiation and promoting muscle precursor cells proliferation. Inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer.1 Publication

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. sequence-specific DNA binding transcription factor activity Source: Ensembl
    3. transcription corepressor activity Source: ProtInc

    GO - Biological processi

    1. central nervous system development Source: Ensembl
    2. epithelial cell differentiation Source: Ensembl
    3. heart development Source: Ensembl
    4. metanephros development Source: Ensembl
    5. multicellular organismal development Source: ProtInc
    6. muscle organ development Source: UniProtKB-KW
    7. negative regulation of osteoblast differentiation Source: Ensembl
    8. negative regulation of sequence-specific DNA binding transcription factor activity Source: GDB
    9. negative regulation of transcription, DNA-templated Source: GDB
    10. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
    11. neuron differentiation Source: Ensembl
    12. notochord development Source: Ensembl
    13. odontogenesis Source: Ensembl
    14. positive regulation of apoptotic process Source: Ensembl
    15. regulation of cell cycle Source: Ensembl
    16. regulation of DNA replication Source: Ensembl
    17. response to wounding Source: Ensembl
    18. transcription, DNA-templated Source: Ensembl

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Biological rhythms, Myogenesis, Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-binding protein inhibitor ID-3
    Alternative name(s):
    Class B basic helix-loop-helix protein 25
    Short name:
    bHLHb25
    Helix-loop-helix protein HEIR-1
    ID-like protein inhibitor HLH 1R21
    Inhibitor of DNA binding 3
    Inhibitor of differentiation 3
    Gene namesi
    Name:ID3
    Synonyms:1R21, BHLHB25, HEIR1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:5362. ID3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. microtubule cytoskeleton Source: HPA
    3. nucleus Source: HPA

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA29610.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 119119DNA-binding protein inhibitor ID-3PRO_0000127247Add
    BLAST

    Proteomic databases

    MaxQBiQ02535.
    PaxDbiQ02535.
    PRIDEiQ02535.

    PTM databases

    PhosphoSiteiQ02535.

    Expressioni

    Tissue specificityi

    Expressed abundantly in lung, kidney and adrenal gland, but not in adult brain.

    Inductioni

    By phorbol 12-myristate 13-acetate (PMA).

    Gene expression databases

    BgeeiQ02535.
    CleanExiHS_ID3.
    GenevestigatoriQ02535.

    Organism-specific databases

    HPAiCAB021353.
    HPA024677.

    Interactioni

    Subunit structurei

    Homodimer, and heterodimer with other HLH proteins. Interacts with COPS5 and COPS7A. Interacts with IFI204. Interacts with GATA4 and NKX2-5. Interacts with ANKRD2; both proteins cooperate in myoblast differentiation By similarity. Interacts with CLOCK and ARNTL/BMAL1.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi109625. 31 interactions.
    DIPiDIP-713N.
    IntActiQ02535. 14 interactions.
    STRINGi9606.ENSP00000363689.

    Structurei

    Secondary structure

    1
    119
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi40 – 423
    Helixi43 – 5412
    Helixi66 – 8116

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2LFHNMR-A/B29-83[»]
    ProteinModelPortaliQ02535.
    SMRiQ02535. Positions 32-83.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini28 – 8053bHLHPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG83634.
    HOGENOMiHOG000234788.
    HOVERGENiHBG009009.
    InParanoidiQ02535.
    KOiK17694.
    OMAiVISKDKR.
    OrthoDBiEOG7X9G8G.
    PhylomeDBiQ02535.
    TreeFamiTF326217.

    Family and domain databases

    Gene3Di4.10.280.10. 1 hit.
    InterProiIPR011598. bHLH_dom.
    IPR026052. DNA-bd_prot-inh.
    [Graphical view]
    PANTHERiPTHR11723. PTHR11723. 1 hit.
    PfamiPF00010. HLH. 1 hit.
    [Graphical view]
    SMARTiSM00353. HLH. 1 hit.
    [Graphical view]
    SUPFAMiSSF47459. SSF47459. 1 hit.
    PROSITEiPS50888. BHLH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q02535-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKALSPVRGC YEAVCCLSER SLAIARGRGK GPAAEEPLSL LDDMNHCYSR    50
    LRELVPGVPR GTQLSQVEIL QRVIDYILDL QVVLAEPAPG PPDGPHLPIQ 100
    TAELTPELVI SNDKRSFCH 119
    Length:119
    Mass (Da):12,999
    Last modified:February 8, 2011 - v2
    Checksum:i7FC38B56B4DCEFBC
    GO

    Sequence cautioni

    The sequence CAA47360.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti105 – 1051T → A.7 Publications
    Corresponds to variant rs11574 [ dbSNP | Ensembl ].
    VAR_016122
    Natural varianti111 – 1111S → A.
    Corresponds to variant rs11542317 [ dbSNP | Ensembl ].
    VAR_030739

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X66924 mRNA. Translation: CAA47360.1. Different initiation.
    X69111 mRNA. Translation: CAA48862.1.
    X73428 Genomic DNA. Translation: CAA51827.1.
    BT006791 mRNA. Translation: AAP35437.1.
    AK290003 mRNA. Translation: BAF82692.1.
    AL021154 Genomic DNA. Translation: CAA15950.1.
    CH471134 Genomic DNA. Translation: EAW95060.1.
    BC003107 mRNA. Translation: AAH03107.1.
    CCDSiCCDS237.1.
    PIRiI37092. S28529.
    RefSeqiNP_002158.3. NM_002167.4.
    UniGeneiHs.76884.

    Genome annotation databases

    EnsembliENST00000374561; ENSP00000363689; ENSG00000117318.
    GeneIDi3399.
    KEGGihsa:3399.
    UCSCiuc001bhh.4. human.

    Polymorphism databases

    DMDMi322510035.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X66924 mRNA. Translation: CAA47360.1 . Different initiation.
    X69111 mRNA. Translation: CAA48862.1 .
    X73428 Genomic DNA. Translation: CAA51827.1 .
    BT006791 mRNA. Translation: AAP35437.1 .
    AK290003 mRNA. Translation: BAF82692.1 .
    AL021154 Genomic DNA. Translation: CAA15950.1 .
    CH471134 Genomic DNA. Translation: EAW95060.1 .
    BC003107 mRNA. Translation: AAH03107.1 .
    CCDSi CCDS237.1.
    PIRi I37092. S28529.
    RefSeqi NP_002158.3. NM_002167.4.
    UniGenei Hs.76884.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2LFH NMR - A/B 29-83 [» ]
    ProteinModelPortali Q02535.
    SMRi Q02535. Positions 32-83.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109625. 31 interactions.
    DIPi DIP-713N.
    IntActi Q02535. 14 interactions.
    STRINGi 9606.ENSP00000363689.

    PTM databases

    PhosphoSitei Q02535.

    Polymorphism databases

    DMDMi 322510035.

    Proteomic databases

    MaxQBi Q02535.
    PaxDbi Q02535.
    PRIDEi Q02535.

    Protocols and materials databases

    DNASUi 3399.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000374561 ; ENSP00000363689 ; ENSG00000117318 .
    GeneIDi 3399.
    KEGGi hsa:3399.
    UCSCi uc001bhh.4. human.

    Organism-specific databases

    CTDi 3399.
    GeneCardsi GC01M023884.
    HGNCi HGNC:5362. ID3.
    HPAi CAB021353.
    HPA024677.
    MIMi 600277. gene.
    neXtProti NX_Q02535.
    PharmGKBi PA29610.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG83634.
    HOGENOMi HOG000234788.
    HOVERGENi HBG009009.
    InParanoidi Q02535.
    KOi K17694.
    OMAi VISKDKR.
    OrthoDBi EOG7X9G8G.
    PhylomeDBi Q02535.
    TreeFami TF326217.

    Miscellaneous databases

    ChiTaRSi ID3. human.
    GeneWikii ID3_(gene).
    GenomeRNAii 3399.
    NextBioi 13432.
    PROi Q02535.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q02535.
    CleanExi HS_ID3.
    Genevestigatori Q02535.

    Family and domain databases

    Gene3Di 4.10.280.10. 1 hit.
    InterProi IPR011598. bHLH_dom.
    IPR026052. DNA-bd_prot-inh.
    [Graphical view ]
    PANTHERi PTHR11723. PTHR11723. 1 hit.
    Pfami PF00010. HLH. 1 hit.
    [Graphical view ]
    SMARTi SM00353. HLH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47459. SSF47459. 1 hit.
    PROSITEi PS50888. BHLH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mutually exclusive expression of a helix-loop-helix gene and N-myc in human neuroblastomas and in normal development."
      Ellmeier W., Aguzzi A., Kleiner E., Kurzbauer R., Weith A.
      EMBO J. 11:2563-2571(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ALA-105.
    2. "An immediate early human gene encodes an Id-like helix-loop-helix protein and is regulated by protein kinase C activation in diverse cell types."
      Deed R.W., Bianchi S.M., Atherton G.T., Johnston D., Santibanez-Koref M., Murphy J.J., Norton J.D.
      Oncogene 8:599-607(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, VARIANT ALA-105.
    3. "Structural organisation and chromosomal mapping of the human Id-3 gene."
      Deed R.W., Hirose T., Mitchell E.L.D., Santibanez-Koref M.F., Norton J.D.
      Gene 151:309-314(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-105.
      Tissue: Blood.
    4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-105.
    5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-105.
      Tissue: Hippocampus.
    6. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ALA-105.
    8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-105.
      Tissue: Skin.
    9. "The transcriptional repressor ID2 can interact with the canonical clock components CLOCK and BMAL1 and mediate inhibitory effects on mPer1 expression."
      Ward S.M., Fernando S.J., Hou T.Y., Duffield G.E.
      J. Biol. Chem. 285:38987-39000(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CLOCK AND ARNTL.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiID3_HUMAN
    AccessioniPrimary (citable) accession number: Q02535
    Secondary accession number(s): A8K1T8, O75641
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 8, 2011
    Last modified: October 1, 2014
    This is version 142 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3