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Q02535

- ID3_HUMAN

UniProt

Q02535 - ID3_HUMAN

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Protein
DNA-binding protein inhibitor ID-3
Gene
ID3, 1R21, BHLHB25, HEIR1
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Involved in myogenesis by inhibiting skeletal muscle and cardiac myocyte differentiation and promoting muscle precursor cells proliferation. Inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer.

GO - Molecular functioni

  1. sequence-specific DNA binding transcription factor activity Source: Ensembl
  2. transcription corepressor activity Source: ProtInc
Complete GO annotation...

GO - Biological processi

  1. central nervous system development Source: Ensembl
  2. epithelial cell differentiation Source: Ensembl
  3. heart development Source: Ensembl
  4. metanephros development Source: Ensembl
  5. multicellular organismal development Source: ProtInc
  6. muscle organ development Source: UniProtKB-KW
  7. negative regulation of osteoblast differentiation Source: Ensembl
  8. negative regulation of sequence-specific DNA binding transcription factor activity Source: GDB
  9. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
  10. negative regulation of transcription, DNA-templated Source: GDB
  11. neuron differentiation Source: Ensembl
  12. notochord development Source: Ensembl
  13. odontogenesis Source: Ensembl
  14. positive regulation of apoptotic process Source: Ensembl
  15. regulation of DNA replication Source: Ensembl
  16. regulation of cell cycle Source: Ensembl
  17. response to wounding Source: Ensembl
  18. transcription, DNA-templated Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Biological rhythms, Myogenesis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding protein inhibitor ID-3
Alternative name(s):
Class B basic helix-loop-helix protein 25
Short name:
bHLHb25
Helix-loop-helix protein HEIR-1
ID-like protein inhibitor HLH 1R21
Inhibitor of DNA binding 3
Inhibitor of differentiation 3
Gene namesi
Name:ID3
Synonyms:1R21, BHLHB25, HEIR1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 1

Organism-specific databases

HGNCiHGNC:5362. ID3.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. microtubule cytoskeleton Source: HPA
  3. nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29610.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 119119DNA-binding protein inhibitor ID-3
PRO_0000127247Add
BLAST

Proteomic databases

MaxQBiQ02535.
PaxDbiQ02535.
PRIDEiQ02535.

PTM databases

PhosphoSiteiQ02535.

Expressioni

Tissue specificityi

Expressed abundantly in lung, kidney and adrenal gland, but not in adult brain.

Inductioni

By phorbol 12-myristate 13-acetate (PMA).

Gene expression databases

BgeeiQ02535.
CleanExiHS_ID3.
GenevestigatoriQ02535.

Organism-specific databases

HPAiCAB021353.
HPA024677.

Interactioni

Subunit structurei

Homodimer, and heterodimer with other HLH proteins. Interacts with COPS5 and COPS7A. Interacts with IFI204. Interacts with GATA4 and NKX2-5. Interacts with ANKRD2; both proteins cooperate in myoblast differentiation By similarity. Interacts with CLOCK and ARNTL/BMAL1.

Protein-protein interaction databases

BioGridi109625. 31 interactions.
DIPiDIP-713N.
IntActiQ02535. 14 interactions.
STRINGi9606.ENSP00000363689.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi40 – 423
Helixi43 – 5412
Helixi66 – 8116

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LFHNMR-A/B29-83[»]
ProteinModelPortaliQ02535.
SMRiQ02535. Positions 32-83.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 8053bHLH
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG83634.
HOGENOMiHOG000234788.
HOVERGENiHBG009009.
InParanoidiQ02535.
KOiK17694.
OMAiVISKDKR.
OrthoDBiEOG7X9G8G.
PhylomeDBiQ02535.
TreeFamiTF326217.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR026052. DNA-bd_prot-inh.
[Graphical view]
PANTHERiPTHR11723. PTHR11723. 1 hit.
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q02535-1 [UniParc]FASTAAdd to Basket

« Hide

MKALSPVRGC YEAVCCLSER SLAIARGRGK GPAAEEPLSL LDDMNHCYSR    50
LRELVPGVPR GTQLSQVEIL QRVIDYILDL QVVLAEPAPG PPDGPHLPIQ 100
TAELTPELVI SNDKRSFCH 119
Length:119
Mass (Da):12,999
Last modified:February 8, 2011 - v2
Checksum:i7FC38B56B4DCEFBC
GO

Sequence cautioni

The sequence CAA47360.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti105 – 1051T → A.7 Publications
Corresponds to variant rs11574 [ dbSNP | Ensembl ].
VAR_016122
Natural varianti111 – 1111S → A.
Corresponds to variant rs11542317 [ dbSNP | Ensembl ].
VAR_030739

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X66924 mRNA. Translation: CAA47360.1. Different initiation.
X69111 mRNA. Translation: CAA48862.1.
X73428 Genomic DNA. Translation: CAA51827.1.
BT006791 mRNA. Translation: AAP35437.1.
AK290003 mRNA. Translation: BAF82692.1.
AL021154 Genomic DNA. Translation: CAA15950.1.
CH471134 Genomic DNA. Translation: EAW95060.1.
BC003107 mRNA. Translation: AAH03107.1.
CCDSiCCDS237.1.
PIRiI37092. S28529.
RefSeqiNP_002158.3. NM_002167.4.
UniGeneiHs.76884.

Genome annotation databases

EnsembliENST00000374561; ENSP00000363689; ENSG00000117318.
GeneIDi3399.
KEGGihsa:3399.
UCSCiuc001bhh.4. human.

Polymorphism databases

DMDMi322510035.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X66924 mRNA. Translation: CAA47360.1 . Different initiation.
X69111 mRNA. Translation: CAA48862.1 .
X73428 Genomic DNA. Translation: CAA51827.1 .
BT006791 mRNA. Translation: AAP35437.1 .
AK290003 mRNA. Translation: BAF82692.1 .
AL021154 Genomic DNA. Translation: CAA15950.1 .
CH471134 Genomic DNA. Translation: EAW95060.1 .
BC003107 mRNA. Translation: AAH03107.1 .
CCDSi CCDS237.1.
PIRi I37092. S28529.
RefSeqi NP_002158.3. NM_002167.4.
UniGenei Hs.76884.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2LFH NMR - A/B 29-83 [» ]
ProteinModelPortali Q02535.
SMRi Q02535. Positions 32-83.
ModBasei Search...

Protein-protein interaction databases

BioGridi 109625. 31 interactions.
DIPi DIP-713N.
IntActi Q02535. 14 interactions.
STRINGi 9606.ENSP00000363689.

PTM databases

PhosphoSitei Q02535.

Polymorphism databases

DMDMi 322510035.

Proteomic databases

MaxQBi Q02535.
PaxDbi Q02535.
PRIDEi Q02535.

Protocols and materials databases

DNASUi 3399.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000374561 ; ENSP00000363689 ; ENSG00000117318 .
GeneIDi 3399.
KEGGi hsa:3399.
UCSCi uc001bhh.4. human.

Organism-specific databases

CTDi 3399.
GeneCardsi GC01M023884.
HGNCi HGNC:5362. ID3.
HPAi CAB021353.
HPA024677.
MIMi 600277. gene.
neXtProti NX_Q02535.
PharmGKBi PA29610.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG83634.
HOGENOMi HOG000234788.
HOVERGENi HBG009009.
InParanoidi Q02535.
KOi K17694.
OMAi VISKDKR.
OrthoDBi EOG7X9G8G.
PhylomeDBi Q02535.
TreeFami TF326217.

Miscellaneous databases

ChiTaRSi ID3. human.
GeneWikii ID3_(gene).
GenomeRNAii 3399.
NextBioi 13432.
PROi Q02535.
SOURCEi Search...

Gene expression databases

Bgeei Q02535.
CleanExi HS_ID3.
Genevestigatori Q02535.

Family and domain databases

Gene3Di 4.10.280.10. 1 hit.
InterProi IPR011598. bHLH_dom.
IPR026052. DNA-bd_prot-inh.
[Graphical view ]
PANTHERi PTHR11723. PTHR11723. 1 hit.
Pfami PF00010. HLH. 1 hit.
[Graphical view ]
SMARTi SM00353. HLH. 1 hit.
[Graphical view ]
SUPFAMi SSF47459. SSF47459. 1 hit.
PROSITEi PS50888. BHLH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mutually exclusive expression of a helix-loop-helix gene and N-myc in human neuroblastomas and in normal development."
    Ellmeier W., Aguzzi A., Kleiner E., Kurzbauer R., Weith A.
    EMBO J. 11:2563-2571(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ALA-105.
  2. "An immediate early human gene encodes an Id-like helix-loop-helix protein and is regulated by protein kinase C activation in diverse cell types."
    Deed R.W., Bianchi S.M., Atherton G.T., Johnston D., Santibanez-Koref M., Murphy J.J., Norton J.D.
    Oncogene 8:599-607(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, VARIANT ALA-105.
  3. "Structural organisation and chromosomal mapping of the human Id-3 gene."
    Deed R.W., Hirose T., Mitchell E.L.D., Santibanez-Koref M.F., Norton J.D.
    Gene 151:309-314(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-105.
    Tissue: Blood.
  4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-105.
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-105.
    Tissue: Hippocampus.
  6. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ALA-105.
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-105.
    Tissue: Skin.
  9. "The transcriptional repressor ID2 can interact with the canonical clock components CLOCK and BMAL1 and mediate inhibitory effects on mPer1 expression."
    Ward S.M., Fernando S.J., Hou T.Y., Duffield G.E.
    J. Biol. Chem. 285:38987-39000(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CLOCK AND ARNTL.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiID3_HUMAN
AccessioniPrimary (citable) accession number: Q02535
Secondary accession number(s): A8K1T8, O75641
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 8, 2011
Last modified: September 3, 2014
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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