Reviewed, UniProtKB/Swiss-Prot Q02527 (MGAT3_RAT)

Names and origin

Protein names

Recommended name:
    Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
    EC=2.4.1.144
Alternative name(s):
    N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III
      Short name=N-acetylglucosaminyltransferase III
      Short name=GlcNAc-T III
      Short name=GNT-III

Gene names

Name:	Mgat3
Synonyms:	Gnt3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	538 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	It is involved in the regulation of the biosynthesis and biological function of glycoprotein oligosaccharides. Catalyzes the addition of N-acetylglucosamine in beta 1-4 linkage to the beta-linked mannose of the trimannosyl core of N-linked sugar chains. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.
Catalytic activity	UDP-N-acetyl-D-glucosamine + beta-D-mannosyl-R = UDP + 4-(N-acetyl-beta-D-glucosaminyl)-beta-D-mannosyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.
Sequence similarities	Belongs to the glycosyltransferase 17 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	protein amino acid N-linked glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-1,4-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 538	538	Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase		PRO_0000188844
Regions
	Topological domain	1 – 7	7	Cytoplasmic Potential
	Transmembrane	8 – 23	16	Signal-anchor for type II membrane protein Potential
	Topological domain	24 – 538	515	Lumenal Potential
	Compositional bias	35 – 86	52	Pro-rich
Amino acid modifications
	Modified residue	265	1	Phosphothreonine By similarity
	Glycosylation	245	1	N-linked (GlcNAc...) Potential
	Glycosylation	263	1	N-linked (GlcNAc...) Potential
	Glycosylation	401	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q02527-1 [UniParc]. Last modified March 15, 2005. Version 2. Checksum: 9DCBE619284D7438	FASTA	538	62,022
	10 20 30 40 50 60 MKMRRYKLFL MFCMAGLCLI SFLHFFKTLS YVTFPRELAS LSPNLISSFF WNNAPVTPQA 70 80 90 100 110 120 SPEPGDPDLL RTPLYSHSPL LQPLSPSKAT EELHRVDFVL PEDTTEYFVR TKAGGVCFKP 130 140 150 160 170 180 GTRMLEKPSP GRTEEKTKVA EGSSVRGPAR RPMRHVLSAR ERLGGRGTRR KWVECVCLPG 190 200 210 220 230 240 WHGPSCGVPT VVQYSNLPTK ERLVPREVPR RVINAININH EFDLLDVRFH ELGDVVDAFV 250 260 270 280 290 300 VCESNFTAYG EPRPLKFREM LTNGTFEYIR HKVLYVFLDH FPPGGRQDGW IADDYLRTFL 310 320 330 340 350 360 TQDGVSRLRN LRPDDVFIID DADEIPARDG VLFLKLYDGW TEPFAFHMRK SLYGFFWKQP 370 380 390 400 410 420 GTLEVVSGCT IDMLQAVYGL DGIRLRRRQY YTMPNFRQYE NRTGHILVQW SLGSPLHFAG 430 440 450 460 470 480 WHCSWCFTPE GIYFKLVSAQ NGDFPRWGDY EDKRDLNYIR SLIRTGGWFD GTQQEYPPAD 490 500 510 520 530 PSEHMYAPKY LLKNYDQFRY LLENPYREPK STVEGGRRNQ GSDGRSSAVR GKLDTTEG

References

[1]

"Purification, cDNA cloning, and expression of UDP-N-acetylglucosamine: beta-D-mannoside beta-1,4N-acetylglucosaminyltransferase III from rat kidney." Nishikawa A., Ihara Y., Hatakeyama M., Kangawa K., Taniguchi N. J. Biol. Chem. 267:18199-18204(1992) [PubMed: 1325461] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 287-297; 447-453 AND 494-509. Strain: Donryu. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D10852 mRNA. Translation: BAA01625.1. Different initiation.
IPI	IPI00207986.
PIR	A43415.
RefSeq	NP_062112.2.
UniGene	Rn.9803
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT17. Glycosyltransferase Family 17.
Genome annotation databases
Ensembl	ENSRNOG00000017434. Rattus norvegicus. [Contig view]
GeneID	29582.
KEGG	rno:29582.
NMPDR	fig|10116.3.peg.27424.
Organism-specific databases
RGD	3084. Mgat3.
Phylogenomic databases
HOVERGEN	Q02527.
OMA	Q02527. QEYPPAD.
Enzyme and pathway databases
BRENDA	2.4.1.144. 248.
Gene expression databases
ArrayExpress	Q02527.
GermOnline	ENSRNOG00000017434. Rattus norvegicus.
Family and domain databases
InterPro	IPR006813. Glyco_trans_17. [Graphical view]
PANTHER	PTHR12224. Glyco_trans_17. 1 hit.
Pfam	PF04724. Glyco_transf_17. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	609694.

Entry information

Entry name

MGAT3_RAT

Accession

Primary (citable) accession number: Q02527

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	March 15, 2005
Last modified:	June 16, 2009
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)