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Protein

Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase

Gene

Mgat3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

It is involved in the regulation of the biosynthesis and biological function of glycoprotein oligosaccharides. Catalyzes the addition of N-acetylglucosamine in beta 1-4 linkage to the beta-linked mannose of the trimannosyl core of N-linked sugar chains. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-mannosyl-R = UDP + 4-(N-acetyl-beta-D-glucosaminyl)-beta-D-mannosyl-R.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.144. 5301.
ReactomeiR-RNO-975574. Reactions specific to the hybrid N-glycan synthesis pathway.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT17. Glycosyltransferase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase (EC:2.4.1.144)
Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III
Short name:
GNT-III
Short name:
GlcNAc-T III
Short name:
N-acetylglucosaminyltransferase III
Gene namesi
Name:Mgat3
Synonyms:Gnt3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi3084. Mgat3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence analysis
Transmembranei8 – 2316Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini24 – 538515LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 538538Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferasePRO_0000188844Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi245 – 2451N-linked (GlcNAc...)Sequence analysis
Glycosylationi263 – 2631N-linked (GlcNAc...)Sequence analysis
Glycosylationi401 – 4011N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ02527.

Expressioni

Gene expression databases

ExpressionAtlasiQ02527. baseline and differential.
GenevisibleiQ02527. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023434.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi35 – 8652Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyltransferase 17 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEQJ. Eukaryota.
ENOG410YQKP. LUCA.
GeneTreeiENSGT00390000008221.
HOGENOMiHOG000113579.
HOVERGENiHBG052468.
InParanoidiQ02527.
KOiK00737.
OMAiYTMPGFR.
OrthoDBiEOG7MPRDD.
PhylomeDBiQ02527.
TreeFamiTF323781.

Family and domain databases

InterProiIPR006813. Glyco_trans_17.
[Graphical view]
PANTHERiPTHR12224. PTHR12224. 2 hits.
PfamiPF04724. Glyco_transf_17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q02527-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMRRYKLFL MFCMAGLCLI SFLHFFKTLS YVTFPRELAS LSPNLISSFF
60 70 80 90 100
WNNAPVTPQA SPEPGDPDLL RTPLYSHSPL LQPLSPSKAT EELHRVDFVL
110 120 130 140 150
PEDTTEYFVR TKAGGVCFKP GTRMLEKPSP GRTEEKTKVA EGSSVRGPAR
160 170 180 190 200
RPMRHVLSAR ERLGGRGTRR KWVECVCLPG WHGPSCGVPT VVQYSNLPTK
210 220 230 240 250
ERLVPREVPR RVINAININH EFDLLDVRFH ELGDVVDAFV VCESNFTAYG
260 270 280 290 300
EPRPLKFREM LTNGTFEYIR HKVLYVFLDH FPPGGRQDGW IADDYLRTFL
310 320 330 340 350
TQDGVSRLRN LRPDDVFIID DADEIPARDG VLFLKLYDGW TEPFAFHMRK
360 370 380 390 400
SLYGFFWKQP GTLEVVSGCT IDMLQAVYGL DGIRLRRRQY YTMPNFRQYE
410 420 430 440 450
NRTGHILVQW SLGSPLHFAG WHCSWCFTPE GIYFKLVSAQ NGDFPRWGDY
460 470 480 490 500
EDKRDLNYIR SLIRTGGWFD GTQQEYPPAD PSEHMYAPKY LLKNYDQFRY
510 520 530
LLENPYREPK STVEGGRRNQ GSDGRSSAVR GKLDTTEG
Length:538
Mass (Da):62,022
Last modified:March 15, 2005 - v2
Checksum:i9DCBE619284D7438
GO

Sequence cautioni

The sequence BAA01625.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10852 mRNA. Translation: BAA01625.1. Different initiation.
PIRiA43415.
RefSeqiNP_062112.2. NM_019239.2.
XP_006242134.1. XM_006242072.2.
XP_006242135.1. XM_006242073.2.
XP_006242136.1. XM_006242074.2.
XP_006242139.1. XM_006242077.2.
UniGeneiRn.9803.

Genome annotation databases

EnsembliENSRNOT00000023434; ENSRNOP00000023434; ENSRNOG00000017434.
GeneIDi29582.
KEGGirno:29582.
UCSCiRGD:3084. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10852 mRNA. Translation: BAA01625.1. Different initiation.
PIRiA43415.
RefSeqiNP_062112.2. NM_019239.2.
XP_006242134.1. XM_006242072.2.
XP_006242135.1. XM_006242073.2.
XP_006242136.1. XM_006242074.2.
XP_006242139.1. XM_006242077.2.
UniGeneiRn.9803.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023434.

Protein family/group databases

CAZyiGT17. Glycosyltransferase Family 17.

Proteomic databases

PaxDbiQ02527.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000023434; ENSRNOP00000023434; ENSRNOG00000017434.
GeneIDi29582.
KEGGirno:29582.
UCSCiRGD:3084. rat.

Organism-specific databases

CTDi4248.
RGDi3084. Mgat3.

Phylogenomic databases

eggNOGiENOG410IEQJ. Eukaryota.
ENOG410YQKP. LUCA.
GeneTreeiENSGT00390000008221.
HOGENOMiHOG000113579.
HOVERGENiHBG052468.
InParanoidiQ02527.
KOiK00737.
OMAiYTMPGFR.
OrthoDBiEOG7MPRDD.
PhylomeDBiQ02527.
TreeFamiTF323781.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.1.144. 5301.
ReactomeiR-RNO-975574. Reactions specific to the hybrid N-glycan synthesis pathway.

Miscellaneous databases

PROiQ02527.

Gene expression databases

ExpressionAtlasiQ02527. baseline and differential.
GenevisibleiQ02527. RN.

Family and domain databases

InterProiIPR006813. Glyco_trans_17.
[Graphical view]
PANTHERiPTHR12224. PTHR12224. 2 hits.
PfamiPF04724. Glyco_transf_17. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Purification, cDNA cloning, and expression of UDP-N-acetylglucosamine: beta-D-mannoside beta-1,4N-acetylglucosaminyltransferase III from rat kidney."
    Nishikawa A., Ihara Y., Hatakeyama M., Kangawa K., Taniguchi N.
    J. Biol. Chem. 267:18199-18204(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 287-297; 447-453 AND 494-509.
    Strain: Donryu.
    Tissue: Kidney.

Entry informationi

Entry nameiMGAT3_RAT
AccessioniPrimary (citable) accession number: Q02527
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: March 15, 2005
Last modified: June 8, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.