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Q02522 (HPRT_LACLA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hypoxanthine-guanine phosphoribosyltransferase

Short name=HGPRT
Short name=HGPRTase
EC=2.4.2.8
Gene names
Name:hpt
Ordered Locus Names:LL0020
ORF Names:L25115
OrganismLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) [Reference proteome] [HAMAP]
Taxonomic identifier272623 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Protein attributes

Sequence length183 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.

GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactor

Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein By similarity.

Pathway

Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the purine/pyrimidine phosphoribosyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 183183Hypoxanthine-guanine phosphoribosyltransferase
PRO_0000139604

Regions

Nucleotide binding103 – 11210IMP By similarity
Nucleotide binding161 – 1622IMP By similarity

Sites

Active site1071Proton acceptor By similarity
Metal binding1621Magnesium By similarity
Binding site1341IMP By similarity
Binding site1561IMP; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q02522 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: A9D903FC9E6EBDB7

FASTA18320,648
        10         20         30         40         50         60 
MLEKNLDKAI EKVLVSEEEI IEKSKELGEI LTKEYEGKNP LVLGILRGSV PFLAELIKHI 

        70         80         90        100        110        120 
DCHLETDFMT VSSYHGGTKS SGEVKLILDV DTAVKGRDIL IVEDIIDTGR TLKYLKELLE 

       130        140        150        160        170        180 
HRGANVKIVT LLDKPEGRIV EIKPDYSGFT IPNEFVVGFG LDYEENYRNL PYVGVLKPEV 


YNK 

« Hide

References

« Hide 'large scale' references
[1]"Isolation of purine auxotrophic mutants of Lactococcus lactis and characterization of the gene hpt encoding hypoxanthine guanine phosphoribosyltransferase."
Nilsson D., Lauridsen A.A.
Mol. Gen. Genet. 235:359-364(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: CHCC285.
[2]"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IL1403.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X67015 Genomic DNA. Translation: CAA47404.1.
X69123 Genomic DNA. Translation: CAA48876.1.
AE005176 Genomic DNA. Translation: AAK04118.1.
PIRD86627.
S30100.
RefSeqNP_266176.1. NC_002662.1.

3D structure databases

ProteinModelPortalQ02522.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272623.L25115.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK04118; AAK04118; L25115.
GeneID1113619.
KEGGlla:L25115.
PATRIC22292150. VBILacLac136773_0022.

Phylogenomic databases

eggNOGCOG0634.
HOGENOMHOG000236520.
KOK00760.
OMAEEDIAGK.
OrthoDBEOG693GNP.

Enzyme and pathway databases

BioCycLLAC272623:GHSH-21-MONOMER.
UniPathwayUPA00591; UER00648.

Family and domain databases

Gene3D3.40.50.2020. 1 hit.
InterProIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMSSF53271. SSF53271. 1 hit.
TIGRFAMsTIGR01203. HGPRTase. 1 hit.
PROSITEPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHPRT_LACLA
AccessionPrimary (citable) accession number: Q02522
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: June 11, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways