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Q02522

- HPRT_LACLA

UniProt

Q02522 - HPRT_LACLA

Protein

Hypoxanthine-guanine phosphoribosyltransferase

Gene

hpt

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
    GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

    Cofactori

    Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei107 – 1071Proton acceptorBy similarity
    Binding sitei134 – 1341IMPBy similarity
    Binding sitei156 – 1561IMP; via carbonyl oxygenBy similarity
    Metal bindingi162 – 1621MagnesiumBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi103 – 11210IMPBy similarity
    Nucleotide bindingi161 – 1622IMPBy similarity

    GO - Molecular functioni

    1. guanine phosphoribosyltransferase activity Source: UniProtKB-EC
    2. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW
    4. nucleotide binding Source: UniProtKB-KW

    GO - Biological processi

    1. IMP salvage Source: UniProtKB-UniPathway
    2. purine ribonucleoside salvage Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Keywords - Ligandi

    Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciLLAC272623:GHSH-21-MONOMER.
    UniPathwayiUPA00591; UER00648.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
    Short name:
    HGPRT
    Short name:
    HGPRTase
    Gene namesi
    Name:hpt
    Ordered Locus Names:LL0020
    ORF Names:L25115
    OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
    Taxonomic identifieri272623 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
    ProteomesiUP000002196: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 183183Hypoxanthine-guanine phosphoribosyltransferasePRO_0000139604Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi272623.L25115.

    Structurei

    3D structure databases

    ProteinModelPortaliQ02522.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0634.
    HOGENOMiHOG000236520.
    KOiK00760.
    OMAiEEDIAGK.
    OrthoDBiEOG693GNP.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    InterProiIPR005904. Hxn_phspho_trans.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF00156. Pribosyltran. 1 hit.
    [Graphical view]
    SUPFAMiSSF53271. SSF53271. 1 hit.
    TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
    PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q02522-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLEKNLDKAI EKVLVSEEEI IEKSKELGEI LTKEYEGKNP LVLGILRGSV    50
    PFLAELIKHI DCHLETDFMT VSSYHGGTKS SGEVKLILDV DTAVKGRDIL 100
    IVEDIIDTGR TLKYLKELLE HRGANVKIVT LLDKPEGRIV EIKPDYSGFT 150
    IPNEFVVGFG LDYEENYRNL PYVGVLKPEV YNK 183
    Length:183
    Mass (Da):20,648
    Last modified:July 1, 1993 - v1
    Checksum:iA9D903FC9E6EBDB7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X67015 Genomic DNA. Translation: CAA47404.1.
    X69123 Genomic DNA. Translation: CAA48876.1.
    AE005176 Genomic DNA. Translation: AAK04118.1.
    PIRiD86627.
    S30100.
    RefSeqiNP_266176.1. NC_002662.1.
    WP_010905038.1. NC_002662.1.

    Genome annotation databases

    EnsemblBacteriaiAAK04118; AAK04118; L25115.
    GeneIDi1113619.
    KEGGilla:L25115.
    PATRICi22292150. VBILacLac136773_0022.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X67015 Genomic DNA. Translation: CAA47404.1 .
    X69123 Genomic DNA. Translation: CAA48876.1 .
    AE005176 Genomic DNA. Translation: AAK04118.1 .
    PIRi D86627.
    S30100.
    RefSeqi NP_266176.1. NC_002662.1.
    WP_010905038.1. NC_002662.1.

    3D structure databases

    ProteinModelPortali Q02522.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272623.L25115.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK04118 ; AAK04118 ; L25115 .
    GeneIDi 1113619.
    KEGGi lla:L25115.
    PATRICi 22292150. VBILacLac136773_0022.

    Phylogenomic databases

    eggNOGi COG0634.
    HOGENOMi HOG000236520.
    KOi K00760.
    OMAi EEDIAGK.
    OrthoDBi EOG693GNP.

    Enzyme and pathway databases

    UniPathwayi UPA00591 ; UER00648 .
    BioCyci LLAC272623:GHSH-21-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    InterProi IPR005904. Hxn_phspho_trans.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53271. SSF53271. 1 hit.
    TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
    PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of purine auxotrophic mutants of Lactococcus lactis and characterization of the gene hpt encoding hypoxanthine guanine phosphoribosyltransferase."
      Nilsson D., Lauridsen A.A.
      Mol. Gen. Genet. 235:359-364(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: CHCC285.
    2. "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
      Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
      Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: IL1403.

    Entry informationi

    Entry nameiHPRT_LACLA
    AccessioniPrimary (citable) accession number: Q02522
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3