Q02521 (SPP2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pre-mRNA-splicing factor SPP2

Alternative name(s):
Spliceosome maturation protein SPP2
Suppressor of PRP protein 2

Gene names

Name:	SPP2
Ordered Locus Names:	YOR148C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	185 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in pre-mRNA splicing; specifically in the final stages of spliceosome maturation. Promotes the first step of splicing. Ref.1
Subunit structure	Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with PRP2. Ref.1 Ref.5 Ref.8
Subcellular location	Cytoplasm. Nucleus Ref.6.
Miscellaneous	Present with 752 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the SPP2 family. Contains 1 G-patch domain.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Cytoplasm Nucleus Spliceosome
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA splicing, via spliceosome Inferred from mutant phenotype Ref.1. Source: SGD
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell spliceosomal complex Inferred from direct assay Ref.1. Source: SGD
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 185	185	Pre-mRNA-splicing factor SPP2		PRO_0000218529
Regions
	Domain	100 – 149	50	G-patch
Amino acid modifications
	Modified residue	117	1	Phosphoserine Ref.9
Experimental info
	Mutagenesis	109	1	L → E or R: Loss of the interaction with PRP2. Ref.8
	Mutagenesis	109	1	L → V: Restores interactions with PRP2 mutants. Ref.8
	Sequence conflict	68 – 69	2	KK → GG in AAA62318. Ref.1
	Sequence conflict	143	1	P → L in AAA62318. Ref.1
	Sequence conflict	149	1	G → V in AAA62318. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q02521 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 3120C3234D178FCD	FASTA	185	20,648
	10 20 30 40 50 60 MSKFSLKLGS KTLKKNISKK TKKKNSLQKA NLFDWDDAET ASLSHKPQSK IKIQSIDKFD 70 80 90 100 110 120 LDEESSSKKK LVIKLSENAD TKKNDAPLVE YVTEKEYNEV PVEEFGDALL RGMGWESDSE 130 140 150 160 170 180 QDSKGDKTQS RNKDVSNVSQ IHPDGLGIGA KLNKAINVEE ASFMPVVKID KITGTKVDDD KKNKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The final stages of spliceosome maturation require Spp2p that can interact with the DEAH box protein Prp2p and promote step 1 of splicing." Roy J., Kim K., Maddock J.R., Anthony J.G., Woolford J.L. Jr. RNA 1:375-390(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH PRP2.
[2]	"Analysis of a 35.6 kb region on the right arm of Saccharomyces cerevisiae chromosome XV." Bordonne R., Camasses A., Madania A., Poch O., Tarassov I.A., Winsor B., Martin R.P. Yeast 13:73-83(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S288c / FY1678.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S288c / FY1678.
[5]	"Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs." Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L. Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY, IDENTIFICATION IN THE CWC COMPLEX.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Interaction between a G-patch protein and a spliceosomal DEXD/H-box ATPase that is critical for splicing." Silverman E.J., Maeda A., Wei J., Smith P., Beggs J.D., Lin R.-J. Mol. Cell. Biol. 24:10101-10110(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PRP2, MUTAGENESIS OF LEU-109.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-117, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U20613 Genomic DNA. Translation: AAA62318.1. U55020 Genomic DNA. Translation: AAC49634.1. Z75056 Genomic DNA. Translation: CAA99354.1. BK006948 Genomic DNA. Translation: DAA10921.1.
PIR	S67036.
RefSeq	NP_014791.1. NM_001183567.1.
3D structure databases
ProteinModelPortal	Q02521.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4140N.
IntAct	Q02521. 4 interactions.
MINT	MINT-485319.
STRING	4932.YOR148C.
Proteomic databases
PaxDb	Q02521.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR148C; YOR148C; YOR148C.
GeneID	854319.
KEGG	sce:YOR148C.
Organism-specific databases
CYGD	YOR148c.
SGD	S000005674. SPP2.
Phylogenomic databases
eggNOG	NOG125961.
OrthoDB	EOG48KVMC.
Gene expression databases
Genevestigator	Q02521.
GermOnline	YOR148C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000467. G_patch_dom. IPR026974. SPP2/Cwf28. [Graphical view]
PANTHER	PTHR15818:SF1. PTHR15818:SF1. 1 hit.
SMART	SM00443. G_patch. 1 hit. [Graphical view]
PROSITE	PS50174. G_PATCH. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	976351.

Entry information

Entry name

SPP2_YEAST

Accession

Primary (citable) accession number: Q02521
Secondary accession number(s): D6W2K5, Q12353

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program