Q02516 (HAP5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional activator HAP5

Gene names

Name:	HAP5
Ordered Locus Names:	YOR358W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts a component of the CCAT-binding factor, which is a transcriptional activator and binds to the upstream activation site (UAS2) of the CYC1 gene and other genes involved in mitochondrial electron transport and activates their expression. Recognizes the sequence 5'-CCAAT-3'. HAP5 is essential for DNA-binding activity. It may be the linchpin that binds to the subunit association domains (SAD) of HAP2 and HAP3 to bring these proteins together.
Subunit structure	Component of the CCAT-binding factor (CBF or HAP complex II), which consists of one copy each of HAP2, HAP3, HAP4 and HAP5. The assembly of the HAP2-HAP3-HAP5 heteromer (HAP complex I) occurs in a one-step pathway and its binding to DNA is a prerequisite for the association of HAP4.
Subcellular location	Nucleus.
Miscellaneous	Present with 450 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the NFYC/HAP5 subunit family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbon catabolite activation of transcription from RNA polymerase II promoter Inferred from mutant phenotype. Source: SGD regulation of cellular respiration Inferred from genetic interaction Ref.1. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	CCAAT-binding factor complex Inferred from direct assay Ref.1. Source: SGD
Molecular function	DNA binding Inferred from physical interaction. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
HAP2	P06774	9	EBI-8165,EBI-8152
HAP3	P13434	5	EBI-8165,EBI-8157

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 242	242	Transcriptional activator HAP5		PRO_0000218260
Amino acid modifications
	Modified residue	7	1	Phosphoserine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	Q02516 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 4104058BF48A4319	FASTA	242	27,676
	10 20 30 40 50 60 MTDRNFSPQQ GQGPQESLPE GPQPSTMIQR EEMNMPRQYS EQQQLQENEG EGENTRLPVS 70 80 90 100 110 120 EEEFRMVQEL QAIQAGHDQA NLPPSGRGSL EGEDNGNSDG ADGEMDEDDE EYDVFRNVGQ 130 140 150 160 170 180 GLVGHYKEIM IRYWQELINE IESTNEPGSE HQDDFKSHSL PFARIRKVMK TDEDVKMISA 190 200 210 220 230 240 EAPIIFAKAC EIFITELTMR AWCVAERNKR RTLQKADIAE ALQKSDMFDF LIDVVPRRPL PQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of yeast HAP5: a novel subunit of a heterotrimeric complex required for CCAAT binding." McNabb D.S., Xing Y., Guarente L. Genes Dev. 9:47-58(1995) [PubMed: 7828851] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 27-242. Strain: ATCC 204508 / S288c.
[2]	McNabb D.S. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"The Saccharomyces cerevisiae Hap5p homolog from fission yeast reveals two conserved domains that are essential for assembly of heterotetrameric CCAAT-binding factor." McNabb D.S., Tseng K.A.-S., Guarente L. Mol. Cell. Biol. 17:7008-7018(1997) [PubMed: 9372932] [Abstract] Cited for: IDENTIFICATION IN THE CCAT-BINDING FACTOR.
[6]	"A multiprotein complex that interacts with RNA polymerase II elongator." Li Y., Takagi Y., Jiang Y., Tokunaga M., Erdjument-Bromage H., Tempst P., Kornberg R.D. J. Biol. Chem. 276:29628-29631(2001) [PubMed: 11390369] [Abstract] Cited for: IDENTIFICATION IN THE CCAT-BINDING FACTOR.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Assembly of the Hap2p/Hap3p/Hap4p/Hap5p-DNA complex in Saccharomyces cerevisiae." McNabb D.S., Pinto I. Eukaryot. Cell 4:1829-1839(2005) [PubMed: 16278450] [Abstract] Cited for: ASSEMBLY OF THE CCAT-BINDING FACTOR.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U19932 Genomic DNA. Translation: AAC49610.1. Z75266 Genomic DNA. Translation: CAA99687.1. BK006948 Genomic DNA. Translation: DAA11119.1.
PIR	S67270.
RefSeq	NP_015003.1. NM_001183778.1.
3D structure databases
ProteinModelPortal	Q02516.
SMR	Q02516. Positions 160-237.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1363N.
IntAct	Q02516. 10 interactions.
MINT	MINT-406466.
STRING	Q02516.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR358W; YOR358W; YOR358W.
GeneID	854540.
KEGG	sce:YOR358W.
NMPDR	fig|4932.3.peg.6122.
Organism-specific databases
CYGD	YOR358w.
SGD	S000005885. HAP5.
Phylogenomic databases
eggNOG	fuNOG09244.
GeneTree	EFGT00050000004185.
OrthoDB	EOG4BS0W7.
Gene expression databases
ArrayExpress	Q02516.
Genevestigator	Q02516.
GermOnline	YOR358W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR009072. Histone-fold. IPR007125. Histone_core_D. [Graphical view]
Gene3D	G3DSA:1.10.20.10. Histone-fold. 1 hit.
Pfam	PF00125. Histone. 1 hit. [Graphical view]
SUPFAM	SSF47113. Histone-fold. 1 hit.
ProtoNet	Search...
Other
NextBio	976943.

Entry information

Entry name

HAP5_YEAST

Accession

Primary (citable) accession number: Q02516
Secondary accession number(s): D6W353, Q08827

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	December 14, 2011
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program