Transcriptional activator HAP5 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

Q02516 (HAP5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 115. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Transcriptional activator HAP5

Gene names

Name:	HAP5
Ordered Locus Names:	YOR358W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts a component of the CCAT-binding factor, which is a transcriptional activator and binds to the upstream activation site (UAS2) of the CYC1 gene and other genes involved in mitochondrial electron transport and activates their expression. Recognizes the sequence 5'-CCAAT-3'. HAP5 is essential for DNA-binding activity. It may be the linchpin that binds to the subunit association domains (SAD) of HAP2 and HAP3 to bring these proteins together.
Subunit structure	Component of the CCAT-binding factor (CBF or HAP complex II), which consists of one copy each of HAP2, HAP3, HAP4 and HAP5. The assembly of the HAP2-HAP3-HAP5 heteromer (HAP complex I) occurs in a one-step pathway and its binding to DNA is a prerequisite for the association of HAP4. Ref.5 Ref.6
Subcellular location	Nucleus.
Miscellaneous	Present with 450 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the NFYC/HAP5 subunit family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbon catabolite activation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 12614847. Source: SGD regulation of cellular respiration Inferred from genetic interaction Ref.1. Source: SGD
Cellular_component	CCAAT-binding factor complex Inferred from direct assay Ref.1. Source: SGD
Molecular_function	DNA binding Inferred from physical interaction PubMed 15343339. Source: SGD RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Inferred from sequence alignment PubMed 16215174. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
HAP2	P06774	9	EBI-8165,EBI-8152
HAP3	P13434	5	EBI-8165,EBI-8157

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 242

242

Transcriptional activator HAP5

PRO_0000218260

Amino acid modifications

Modified residue

Phosphoserine Ref.9

Sequences

Sequence

Length

Mass (Da)

Tools

Q02516 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 4104058BF48A4319
Show »

FASTA

242

27,676

        10         20         30         40         50         60 
MTDRNFSPQQ GQGPQESLPE GPQPSTMIQR EEMNMPRQYS EQQQLQENEG EGENTRLPVS 

        70         80         90        100        110        120 
EEEFRMVQEL QAIQAGHDQA NLPPSGRGSL EGEDNGNSDG ADGEMDEDDE EYDVFRNVGQ 

       130        140        150        160        170        180 
GLVGHYKEIM IRYWQELINE IESTNEPGSE HQDDFKSHSL PFARIRKVMK TDEDVKMISA 

       190        200        210        220        230        240 
EAPIIFAKAC EIFITELTMR AWCVAERNKR RTLQKADIAE ALQKSDMFDF LIDVVPRRPL 


PQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of yeast HAP5: a novel subunit of a heterotrimeric complex required for CCAAT binding." McNabb D.S., Xing Y., Guarente L. Genes Dev. 9:47-58(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 27-242. Strain: ATCC 204508 / S288c.
[2]	McNabb D.S. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"The Saccharomyces cerevisiae Hap5p homolog from fission yeast reveals two conserved domains that are essential for assembly of heterotetrameric CCAAT-binding factor." McNabb D.S., Tseng K.A.-S., Guarente L. Mol. Cell. Biol. 17:7008-7018(1997) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE CCAT-BINDING FACTOR.
[6]	"A multiprotein complex that interacts with RNA polymerase II elongator." Li Y., Takagi Y., Jiang Y., Tokunaga M., Erdjument-Bromage H., Tempst P., Kornberg R.D. J. Biol. Chem. 276:29628-29631(2001) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE CCAT-BINDING FACTOR.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Assembly of the Hap2p/Hap3p/Hap4p/Hap5p-DNA complex in Saccharomyces cerevisiae." McNabb D.S., Pinto I. Eukaryot. Cell 4:1829-1839(2005) [PubMed] [Europe PMC] [Abstract] Cited for: ASSEMBLY OF THE CCAT-BINDING FACTOR.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U19932 Genomic DNA. Translation: AAC49610.1. Z75266 Genomic DNA. Translation: CAA99687.1. BK006948 Genomic DNA. Translation: DAA11119.1.
PIR	S67270.
RefSeq	NP_015003.1. NM_001183778.1.
3D structure databases
ProteinModelPortal	Q02516.
SMR	Q02516. Positions 117-237.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1363N.
IntAct	Q02516. 10 interactions.
MINT	MINT-406466.
STRING	4932.YOR358W.
Proteomic databases
PaxDb	Q02516.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR358W; YOR358W; YOR358W.
GeneID	854540.
KEGG	sce:YOR358W.
Organism-specific databases
CYGD	YOR358w.
SGD	S000005885. HAP5.
Phylogenomic databases
eggNOG	COG5208.
GeneTree	ENSGT00530000063560.
OrthoDB	EOG4BS0W7.
Gene expression databases
Genevestigator	Q02516.
GermOnline	YOR358W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.20.10. 1 hit.
InterPro	IPR009072. Histone-fold. IPR007125. Histone_core_D. IPR027170. NFYC/HAP5_su. [Graphical view]
PANTHER	PTHR10252:SF8. PTHR10252:SF8. 1 hit.
Pfam	PF00125. Histone. 1 hit. [Graphical view]
SUPFAM	SSF47113. Histone-fold. 1 hit.
ProtoNet	Search...
Other
NextBio	976943.

Entry information

Entry name

HAP5_YEAST

Accession

Primary (citable) accession number: Q02516
Secondary accession number(s): D6W353, Q08827

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents