Reviewed, UniProtKB/Swiss-Prot Q02486 (ABF2_YEAST)

Names and origin

Protein names

Recommended name:
    ARS-binding factor 2, mitochondrial

Gene names

Name:	ABF2
Synonyms:	HIM1
Ordered Locus Names:	YMR072W
ORF Names:	YM9916.11

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Specific binding to the autonomously replicating sequence 1 (ARS1). Interaction with regulatory regions: probably involved in compacting the mitochondrial genome. It might play a positive role in gene expression and replication.
Subcellular location	Mitochondrion. Nucleus Probable.
Miscellaneous	Present with 3810 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Contains 2 HMG box DNA-binding domains.

Ontologies

Keywords
Cellular component	Mitochondrion Nucleus
Domain	Repeat Transit peptide
Ligand	DNA-binding
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	mitochondrial genome maintenance Inferred from mutant phenotype. Source: SGD mitochondrion inheritance Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrial chromosome Inferred from direct assay. Source: SGD nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA bending activity Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MLH1	P38920	1	EBI-2028,EBI-11003

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 26	26	Mitochondrion Ref.1
	Chain	27 – 183	157	ARS-binding factor 2, mitochondrial		PRO_0000013477
Regions
	DNA binding	43 – 111	69	HMG box 1
	DNA binding	116 – 183	68	HMG box 2

Sequences

	Sequence		Length	Mass (Da)
	Q02486-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: D280A7D2B675B5B1	FASTA	183	21,562
	10 20 30 40 50 60 MNSYSLLTRS FHESSKPLFN LASTLLKASK RTQLRNELIK QGPKRPTSAY FLYLQDHRSQ 70 80 90 100 110 120 FVKENPTLRP AEISKIAGEK WQNLEADIKE KYISERKKLY SEYQKAKKEF DEKLPPKKPA 130 140 150 160 170 180 GPFIKYANEV RSQVFAQHPD KSQLDLMKII GDKWQSLDQS IKDKYIQEYK KAIQEYNARY PLN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A close relative of the nuclear, chromosomal high-mobility group protein HMG1 in yeast mitochondria." Diffley J.F.X., Stillman B. Proc. Natl. Acad. Sci. U.S.A. 88:7864-7868(1991) [PubMed: 1881919] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 27-56.
[2]	"Molecular cloning and characterization of yeast CDRP1: an HMG protein which recognizes cisplatin-damaged DNA and confers drug sensitivity." Sun C.L., Lee F.J., Lin-Chao S., Chao C.K. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M73753 Genomic DNA. Translation: AAA73079.2. Sequence problems. AJ223169 mRNA. Translation: CAA11146.1. Z48952 Genomic DNA. Translation: CAA88797.1. AY557967 Genomic DNA. Translation: AAS56293.1.
PIR	A41302.
RefSeq	NP_013788.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5492N.
IntAct	Q02486. 148 interactions.
STRING	Q02486.
Proteomic databases
PeptideAtlas	Q02486.
Genome annotation databases
Ensembl	YMR072W; YMR072W; YMR072W; Saccharomyces cerevisiae. [Genome view]
GeneID	855094.
KEGG	sce:YMR072W.
NMPDR	fig|4932.3.peg.4835.
Organism-specific databases
CYGD	YMR072w.
SGD	S000004676. ABF2.
Phylogenomic databases
OMA	DHICASH.
OrthoDB	EOG973R8Q.
Gene expression databases
ArrayExpress	Q02486.
Genevestigator	Q02486.
GermOnline	YMR072W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000910. HMG_HMG1/HMG2. IPR009071. HMG_superfamily. [Graphical view]
Gene3D	G3DSA:1.10.30.10. HMG-box. 2 hits.
Pfam	PF00505. HMG_box. 2 hits. [Graphical view]
SMART	SM00398. HMG. 2 hits. [Graphical view]
PROSITE	PS50118. HMG_BOX_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	978405.

Entry information

Entry name

ABF2_YEAST

Accession

Primary (citable) accession number: Q02486
Secondary accession number(s): Q712M5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	December 15, 2009
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)