Q02486 (ABF2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ARS-binding factor 2, mitochondrial

Gene names

Name:	ABF2
Synonyms:	HIM1
Ordered Locus Names:	YMR072W
ORF Names:	YM9916.11

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Specific binding to the autonomously replicating sequence 1 (ARS1). Interaction with regulatory regions: probably involved in compacting the mitochondrial genome. It might play a positive role in gene expression and replication.
Subcellular location	Mitochondrion. Nucleus Probable.
Miscellaneous	Present with 3810 molecules/cell in log phase SD medium.
Sequence similarities	Contains 2 HMG box DNA-binding domains.

Ontologies

Keywords
Cellular component	Mitochondrion Nucleus
Domain	Repeat Transit peptide
Ligand	DNA-binding
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial DNA packaging Inferred from mutant phenotype PubMed 21282901. Source: SGD mitochondrion inheritance Inferred from mutant phenotype PubMed 9581629. Source: SGD
Cellular_component	mitochondrial chromosome Inferred from direct assay PubMed 9581629. Source: SGD nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding, bending Inferred from direct assay PubMed 1737790. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 26	26	Mitochondrion Ref.1
	Chain	27 – 183	157	ARS-binding factor 2, mitochondrial		PRO_0000013477
Regions
	DNA binding	43 – 111	69	HMG box 1
	DNA binding	116 – 183	68	HMG box 2

Sequences

	Sequence		Length	Mass (Da)
	Q02486 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: D280A7D2B675B5B1	FASTA	183	21,562
	10 20 30 40 50 60 MNSYSLLTRS FHESSKPLFN LASTLLKASK RTQLRNELIK QGPKRPTSAY FLYLQDHRSQ 70 80 90 100 110 120 FVKENPTLRP AEISKIAGEK WQNLEADIKE KYISERKKLY SEYQKAKKEF DEKLPPKKPA 130 140 150 160 170 180 GPFIKYANEV RSQVFAQHPD KSQLDLMKII GDKWQSLDQS IKDKYIQEYK KAIQEYNARY PLN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A close relative of the nuclear, chromosomal high-mobility group protein HMG1 in yeast mitochondria." Diffley J.F.X., Stillman B. Proc. Natl. Acad. Sci. U.S.A. 88:7864-7868(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 27-56.
[2]	"Molecular cloning and characterization of yeast CDRP1: an HMG protein which recognizes cisplatin-damaged DNA and confers drug sensitivity." Sun C.L., Lee F.J., Lin-Chao S., Chao C.K. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M73753 Genomic DNA. Translation: AAA73079.2. Sequence problems. AJ223169 mRNA. Translation: CAA11146.1. Z48952 Genomic DNA. Translation: CAA88797.1. AY557967 Genomic DNA. Translation: AAS56293.1. BK006946 Genomic DNA. Translation: DAA09970.1.
PIR	A41302.
RefSeq	NP_013788.1. NM_001182570.1.
3D structure databases
ProteinModelPortal	Q02486.
SMR	Q02486. Positions 42-179.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5492N.
IntAct	Q02486. 29 interactions.
MINT	MINT-505363.
STRING	4932.YMR072W.
Proteomic databases
PaxDb	Q02486.
PeptideAtlas	Q02486.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR072W; YMR072W; YMR072W.
GeneID	855094.
KEGG	sce:YMR072W.
Organism-specific databases
CYGD	YMR072w.
SGD	S000004676. ABF2.
Phylogenomic databases
eggNOG	COG5648.
GeneTree	ENSGT00680000100562.
OMA	FHESSKP.
OrthoDB	EOG483HF0.
Enzyme and pathway databases
BioCyc	YEAST:G3O-32774-MONOMER.
Gene expression databases
Genevestigator	Q02486.
GermOnline	YMR072W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.30.10. 2 hits.
InterPro	IPR009071. HMG_box_dom. [Graphical view]
Pfam	PF00505. HMG_box. 2 hits. [Graphical view]
SMART	SM00398. HMG. 2 hits. [Graphical view]
SUPFAM	SSF47095. HMG-box. 2 hits.
PROSITE	PS50118. HMG_BOX_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	978405.

Entry information

Entry name

ABF2_YEAST

Accession

Primary (citable) accession number: Q02486
Secondary accession number(s): D6VZP6, Q712M5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	May 29, 2013
This is version 118 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program