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Protein

ARS-binding factor 2, mitochondrial

Gene

ABF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specific binding to the autonomously replicating sequence 1 (ARS1). Interaction with regulatory regions: probably involved in compacting the mitochondrial genome. It might play a positive role in gene expression and replication.

Miscellaneous

Present with 3810 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi43 – 111HMG box 1PROSITE-ProRule annotationAdd BLAST69
DNA bindingi116 – 183HMG box 2PROSITE-ProRule annotationAdd BLAST68

GO - Molecular functioni

  • DNA binding Source: SGD
  • DNA binding, bending Source: SGD

GO - Biological processi

  • mitochondrial DNA packaging Source: SGD
  • mitochondrial genome maintenance Source: SGD
  • mitochondrion inheritance Source: SGD

Keywordsi

Molecular functionDNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32774-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ARS-binding factor 2, mitochondrial
Gene namesi
Name:ABF2
Synonyms:HIM1
Ordered Locus Names:YMR072W
ORF Names:YM9916.11
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR072W.
SGDiS000004676. ABF2.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial chromosome Source: SGD
  • mitochondrial nucleoid Source: SGD
  • nucleus Source: UniProtKB-SubCell

Keywords - Cellular componenti

Mitochondrion, Nucleus

Pathology & Biotechi

Disruption phenotypei

Has a temperature-sensive growth defect in glucose-rich medium, with slower growth and smaller colonies at 37 degrees Celsius but not 30 degrees Celsius; the phenotype is suppressed by overexpression of YHM2.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 26Mitochondrion1 PublicationAdd BLAST26
ChainiPRO_000001347727 – 183ARS-binding factor 2, mitochondrialAdd BLAST157

Proteomic databases

MaxQBiQ02486.
PRIDEiQ02486.

Interactioni

Protein-protein interaction databases

BioGridi35247. 110 interactors.
DIPiDIP-5492N.
IntActiQ02486. 30 interactors.
MINTiMINT-505363.

Structurei

Secondary structure

1183
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi30 – 40Combined sources11
Helixi49 – 64Combined sources16
Helixi70 – 83Combined sources14
Helixi86 – 113Combined sources28
Helixi122 – 137Combined sources16
Helixi143 – 156Combined sources14
Helixi159 – 179Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5JGHX-ray2.60A/D/G/J27-183[»]
5JH0X-ray2.18A/D27-183[»]
ProteinModelPortaliQ02486.
SMRiQ02486.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

GeneTreeiENSGT00730000113246.
InParanoidiQ02486.
KOiK11830.
OMAiWKALSPG.
OrthoDBiEOG092C5ER2.

Family and domain databases

Gene3Di1.10.30.10. 2 hits.
InterProiView protein in InterPro
IPR009071. HMG_box_dom.
IPR033311. Ixr1/Abf2.
PANTHERiPTHR13711:SF275. PTHR13711:SF275. 1 hit.
PfamiView protein in Pfam
PF00505. HMG_box. 2 hits.
SMARTiView protein in SMART
SM00398. HMG. 2 hits.
SUPFAMiSSF47095. SSF47095. 2 hits.
PROSITEiView protein in PROSITE
PS50118. HMG_BOX_2. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02486-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSYSLLTRS FHESSKPLFN LASTLLKASK RTQLRNELIK QGPKRPTSAY
60 70 80 90 100
FLYLQDHRSQ FVKENPTLRP AEISKIAGEK WQNLEADIKE KYISERKKLY
110 120 130 140 150
SEYQKAKKEF DEKLPPKKPA GPFIKYANEV RSQVFAQHPD KSQLDLMKII
160 170 180
GDKWQSLDQS IKDKYIQEYK KAIQEYNARY PLN
Length:183
Mass (Da):21,562
Last modified:July 1, 1993 - v1
Checksum:iD280A7D2B675B5B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73753 Genomic DNA. Translation: AAA73079.2. Sequence problems.
AJ223169 mRNA. Translation: CAA11146.1.
Z48952 Genomic DNA. Translation: CAA88797.1.
AY557967 Genomic DNA. Translation: AAS56293.1.
BK006946 Genomic DNA. Translation: DAA09970.1.
PIRiA41302.
RefSeqiNP_013788.1. NM_001182570.1.

Genome annotation databases

EnsemblFungiiYMR072W; YMR072W; YMR072W.
GeneIDi855094.
KEGGisce:YMR072W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73753 Genomic DNA. Translation: AAA73079.2. Sequence problems.
AJ223169 mRNA. Translation: CAA11146.1.
Z48952 Genomic DNA. Translation: CAA88797.1.
AY557967 Genomic DNA. Translation: AAS56293.1.
BK006946 Genomic DNA. Translation: DAA09970.1.
PIRiA41302.
RefSeqiNP_013788.1. NM_001182570.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5JGHX-ray2.60A/D/G/J27-183[»]
5JH0X-ray2.18A/D27-183[»]
ProteinModelPortaliQ02486.
SMRiQ02486.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35247. 110 interactors.
DIPiDIP-5492N.
IntActiQ02486. 30 interactors.
MINTiMINT-505363.

Proteomic databases

MaxQBiQ02486.
PRIDEiQ02486.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR072W; YMR072W; YMR072W.
GeneIDi855094.
KEGGisce:YMR072W.

Organism-specific databases

EuPathDBiFungiDB:YMR072W.
SGDiS000004676. ABF2.

Phylogenomic databases

GeneTreeiENSGT00730000113246.
InParanoidiQ02486.
KOiK11830.
OMAiWKALSPG.
OrthoDBiEOG092C5ER2.

Enzyme and pathway databases

BioCyciYEAST:G3O-32774-MONOMER.

Miscellaneous databases

ChiTaRSiABF2. yeast.
PROiPR:Q02486.

Family and domain databases

Gene3Di1.10.30.10. 2 hits.
InterProiView protein in InterPro
IPR009071. HMG_box_dom.
IPR033311. Ixr1/Abf2.
PANTHERiPTHR13711:SF275. PTHR13711:SF275. 1 hit.
PfamiView protein in Pfam
PF00505. HMG_box. 2 hits.
SMARTiView protein in SMART
SM00398. HMG. 2 hits.
SUPFAMiSSF47095. SSF47095. 2 hits.
PROSITEiView protein in PROSITE
PS50118. HMG_BOX_2. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiABF2_YEAST
AccessioniPrimary (citable) accession number: Q02486
Secondary accession number(s): D6VZP6, Q712M5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: May 10, 2017
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.