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Protein

ARS-binding factor 2, mitochondrial

Gene

ABF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specific binding to the autonomously replicating sequence 1 (ARS1). Interaction with regulatory regions: probably involved in compacting the mitochondrial genome. It might play a positive role in gene expression and replication.

Miscellaneous

Present with 3810 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi43 – 111HMG box 1PROSITE-ProRule annotationAdd BLAST69
DNA bindingi116 – 183HMG box 2PROSITE-ProRule annotationAdd BLAST68

GO - Molecular functioni

  • DNA binding Source: SGD
  • DNA binding, bending Source: SGD

GO - Biological processi

  • mitochondrial DNA packaging Source: SGD
  • mitochondrial genome maintenance Source: SGD
  • mitochondrion inheritance Source: SGD

Keywordsi

Molecular functionDNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32774-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
ARS-binding factor 2, mitochondrial
Gene namesi
Name:ABF2
Synonyms:HIM1
Ordered Locus Names:YMR072W
ORF Names:YM9916.11
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR072W
SGDiS000004676 ABF2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion, Nucleus

Pathology & Biotechi

Disruption phenotypei

Has a temperature-sensitive growth defect in glucose-rich medium, with slower growth and smaller colonies at 37 degrees Celsius but not 30 degrees Celsius; the phenotype is suppressed by overexpression of YHM2.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 26Mitochondrion1 PublicationAdd BLAST26
ChainiPRO_000001347727 – 183ARS-binding factor 2, mitochondrialAdd BLAST157

Proteomic databases

MaxQBiQ02486
PaxDbiQ02486
PRIDEiQ02486

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35247, 132 interactors
DIPiDIP-5492N
IntActiQ02486, 36 interactors
MINTiQ02486
STRINGi4932.YMR072W

Structurei

Secondary structure

1183
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi30 – 40Combined sources11
Helixi49 – 64Combined sources16
Helixi70 – 83Combined sources14
Helixi86 – 113Combined sources28
Helixi122 – 137Combined sources16
Helixi143 – 156Combined sources14
Helixi159 – 179Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5JGHX-ray2.60A/D/G/J27-183[»]
5JH0X-ray2.18A/D27-183[»]
ProteinModelPortaliQ02486
SMRiQ02486
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

GeneTreeiENSGT00730000113246
InParanoidiQ02486
KOiK11830
OMAiYSQDKRP
OrthoDBiEOG092C5ER2

Family and domain databases

Gene3Di1.10.30.10, 2 hits
InterProiView protein in InterPro
IPR009071 HMG_box_dom
IPR036910 HMG_box_dom_sf
IPR033311 Ixr1/Abf2
PANTHERiPTHR13711:SF168 PTHR13711:SF168, 1 hit
PfamiView protein in Pfam
PF00505 HMG_box, 2 hits
SMARTiView protein in SMART
SM00398 HMG, 2 hits
SUPFAMiSSF47095 SSF47095, 2 hits
PROSITEiView protein in PROSITE
PS50118 HMG_BOX_2, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02486-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSYSLLTRS FHESSKPLFN LASTLLKASK RTQLRNELIK QGPKRPTSAY
60 70 80 90 100
FLYLQDHRSQ FVKENPTLRP AEISKIAGEK WQNLEADIKE KYISERKKLY
110 120 130 140 150
SEYQKAKKEF DEKLPPKKPA GPFIKYANEV RSQVFAQHPD KSQLDLMKII
160 170 180
GDKWQSLDQS IKDKYIQEYK KAIQEYNARY PLN
Length:183
Mass (Da):21,562
Last modified:July 1, 1993 - v1
Checksum:iD280A7D2B675B5B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73753 Genomic DNA Translation: AAA73079.2 Sequence problems.
AJ223169 mRNA Translation: CAA11146.1
Z48952 Genomic DNA Translation: CAA88797.1
AY557967 Genomic DNA Translation: AAS56293.1
BK006946 Genomic DNA Translation: DAA09970.1
PIRiA41302
RefSeqiNP_013788.1, NM_001182570.1

Genome annotation databases

EnsemblFungiiYMR072W; YMR072W; YMR072W
GeneIDi855094
KEGGisce:YMR072W

Similar proteinsi

Entry informationi

Entry nameiABF2_YEAST
AccessioniPrimary (citable) accession number: Q02486
Secondary accession number(s): D6VZP6, Q712M5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: March 28, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health