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Protein

ARS-binding factor 2, mitochondrial

Gene

ABF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specific binding to the autonomously replicating sequence 1 (ARS1). Interaction with regulatory regions: probably involved in compacting the mitochondrial genome. It might play a positive role in gene expression and replication.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi43 – 11169HMG box 1PROSITE-ProRule annotationAdd
BLAST
DNA bindingi116 – 18368HMG box 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: SGD
  • DNA binding, bending Source: SGD

GO - Biological processi

  • mitochondrial DNA packaging Source: SGD
  • mitochondrial genome maintenance Source: SGD
  • mitochondrion inheritance Source: SGD
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32774-MONOMER.
ReactomeiREACT_348300. Activation of DNA fragmentation factor.

Names & Taxonomyi

Protein namesi
Recommended name:
ARS-binding factor 2, mitochondrial
Gene namesi
Name:ABF2
Synonyms:HIM1
Ordered Locus Names:YMR072W
ORF Names:YM9916.11
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIII

Organism-specific databases

CYGDiYMR072w.
EuPathDBiFungiDB:YMR072W.
SGDiS000004676. ABF2.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial chromosome Source: SGD
  • mitochondrial nucleoid Source: SGD
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Nucleus

Pathology & Biotechi

Disruption phenotypei

Has a temperature-sensive growth defect in glucose-rich medium, with slower growth and smaller colonies at 37 degrees Celsius but not 30 degrees Celsius; the phenotype is suppressed by overexpression of YHM2.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2626Mitochondrion1 PublicationAdd
BLAST
Chaini27 – 183157ARS-binding factor 2, mitochondrialPRO_0000013477Add
BLAST

Proteomic databases

MaxQBiQ02486.
PaxDbiQ02486.
PeptideAtlasiQ02486.

Interactioni

Protein-protein interaction databases

BioGridi35247. 76 interactions.
DIPiDIP-5492N.
IntActiQ02486. 30 interactions.
MINTiMINT-505363.
STRINGi4932.YMR072W.

Structurei

3D structure databases

ProteinModelPortaliQ02486.
SMRiQ02486. Positions 42-179.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 2 HMG box DNA-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiCOG5648.
GeneTreeiENSGT00730000113246.
InParanoidiQ02486.
KOiK11830.
OMAiDHICASH.
OrthoDBiEOG70KH25.

Family and domain databases

Gene3Di1.10.30.10. 2 hits.
InterProiIPR009071. HMG_box_dom.
[Graphical view]
PfamiPF00505. HMG_box. 2 hits.
[Graphical view]
SMARTiSM00398. HMG. 2 hits.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 2 hits.
PROSITEiPS50118. HMG_BOX_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02486-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSYSLLTRS FHESSKPLFN LASTLLKASK RTQLRNELIK QGPKRPTSAY
60 70 80 90 100
FLYLQDHRSQ FVKENPTLRP AEISKIAGEK WQNLEADIKE KYISERKKLY
110 120 130 140 150
SEYQKAKKEF DEKLPPKKPA GPFIKYANEV RSQVFAQHPD KSQLDLMKII
160 170 180
GDKWQSLDQS IKDKYIQEYK KAIQEYNARY PLN
Length:183
Mass (Da):21,562
Last modified:July 1, 1993 - v1
Checksum:iD280A7D2B675B5B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73753 Genomic DNA. Translation: AAA73079.2. Sequence problems.
AJ223169 mRNA. Translation: CAA11146.1.
Z48952 Genomic DNA. Translation: CAA88797.1.
AY557967 Genomic DNA. Translation: AAS56293.1.
BK006946 Genomic DNA. Translation: DAA09970.1.
PIRiA41302.
RefSeqiNP_013788.1. NM_001182570.1.

Genome annotation databases

EnsemblFungiiYMR072W; YMR072W; YMR072W.
GeneIDi855094.
KEGGisce:YMR072W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73753 Genomic DNA. Translation: AAA73079.2. Sequence problems.
AJ223169 mRNA. Translation: CAA11146.1.
Z48952 Genomic DNA. Translation: CAA88797.1.
AY557967 Genomic DNA. Translation: AAS56293.1.
BK006946 Genomic DNA. Translation: DAA09970.1.
PIRiA41302.
RefSeqiNP_013788.1. NM_001182570.1.

3D structure databases

ProteinModelPortaliQ02486.
SMRiQ02486. Positions 42-179.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35247. 76 interactions.
DIPiDIP-5492N.
IntActiQ02486. 30 interactions.
MINTiMINT-505363.
STRINGi4932.YMR072W.

Proteomic databases

MaxQBiQ02486.
PaxDbiQ02486.
PeptideAtlasiQ02486.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR072W; YMR072W; YMR072W.
GeneIDi855094.
KEGGisce:YMR072W.

Organism-specific databases

CYGDiYMR072w.
EuPathDBiFungiDB:YMR072W.
SGDiS000004676. ABF2.

Phylogenomic databases

eggNOGiCOG5648.
GeneTreeiENSGT00730000113246.
InParanoidiQ02486.
KOiK11830.
OMAiDHICASH.
OrthoDBiEOG70KH25.

Enzyme and pathway databases

BioCyciYEAST:G3O-32774-MONOMER.
ReactomeiREACT_348300. Activation of DNA fragmentation factor.

Miscellaneous databases

ChiTaRSiABF2. yeast.
NextBioi978405.
PROiQ02486.

Family and domain databases

Gene3Di1.10.30.10. 2 hits.
InterProiIPR009071. HMG_box_dom.
[Graphical view]
PfamiPF00505. HMG_box. 2 hits.
[Graphical view]
SMARTiSM00398. HMG. 2 hits.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 2 hits.
PROSITEiPS50118. HMG_BOX_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A close relative of the nuclear, chromosomal high-mobility group protein HMG1 in yeast mitochondria."
    Diffley J.F.X., Stillman B.
    Proc. Natl. Acad. Sci. U.S.A. 88:7864-7868(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 27-56.
  2. "Molecular cloning and characterization of yeast CDRP1: an HMG protein which recognizes cisplatin-damaged DNA and confers drug sensitivity."
    Sun C.L., Lee F.J., Lin-Chao S., Chao C.K.
    Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "A novel DNA-binding protein bound to the mitochondrial inner membrane restores the null mutation of mitochondrial histone Abf2p in Saccharomyces cerevisiae."
    Cho J.H., Ha S.J., Kao L.R., Megraw T.L., Chae C.-B.
    Mol. Cell. Biol. 18:5712-5723(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiABF2_YEAST
AccessioniPrimary (citable) accession number: Q02486
Secondary accession number(s): D6VZP6, Q712M5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: June 24, 2015
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3810 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.