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Q02486

- ABF2_YEAST

UniProt

Q02486 - ABF2_YEAST

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Protein

ARS-binding factor 2, mitochondrial

Gene

ABF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Specific binding to the autonomously replicating sequence 1 (ARS1). Interaction with regulatory regions: probably involved in compacting the mitochondrial genome. It might play a positive role in gene expression and replication.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi43 – 11169HMG box 1PROSITE-ProRule annotationAdd
BLAST
DNA bindingi116 – 18368HMG box 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: SGD
  2. DNA binding, bending Source: SGD

GO - Biological processi

  1. mitochondrial DNA packaging Source: SGD
  2. mitochondrial genome maintenance Source: SGD
  3. mitochondrion inheritance Source: SGD
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32774-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ARS-binding factor 2, mitochondrial
Gene namesi
Name:ABF2
Synonyms:HIM1
Ordered Locus Names:YMR072W
ORF Names:YM9916.11
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR072w.
SGDiS000004676. ABF2.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial chromosome Source: SGD
  2. mitochondrial nucleoid Source: SGD
  3. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Nucleus

Pathology & Biotechi

Disruption phenotypei

Has a temperature-sensive growth defect in glucose-rich medium, with slower growth and smaller colonies at 37 degrees Celsius but not 30 degrees Celsius; the phenotype is suppressed by overexpression of YHM2.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2626Mitochondrion1 PublicationAdd
BLAST
Chaini27 – 183157ARS-binding factor 2, mitochondrialPRO_0000013477Add
BLAST

Proteomic databases

MaxQBiQ02486.
PaxDbiQ02486.
PeptideAtlasiQ02486.

Expressioni

Gene expression databases

GenevestigatoriQ02486.

Interactioni

Protein-protein interaction databases

BioGridi35247. 75 interactions.
DIPiDIP-5492N.
IntActiQ02486. 30 interactions.
MINTiMINT-505363.
STRINGi4932.YMR072W.

Structurei

3D structure databases

ProteinModelPortaliQ02486.
SMRiQ02486. Positions 42-179.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 2 HMG box DNA-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiCOG5648.
GeneTreeiENSGT00730000113246.
InParanoidiQ02486.
KOiK11830.
OMAiDHICASH.
OrthoDBiEOG70KH25.

Family and domain databases

Gene3Di1.10.30.10. 2 hits.
InterProiIPR009071. HMG_box_dom.
[Graphical view]
PfamiPF00505. HMG_box. 2 hits.
[Graphical view]
SMARTiSM00398. HMG. 2 hits.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 2 hits.
PROSITEiPS50118. HMG_BOX_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02486-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNSYSLLTRS FHESSKPLFN LASTLLKASK RTQLRNELIK QGPKRPTSAY
60 70 80 90 100
FLYLQDHRSQ FVKENPTLRP AEISKIAGEK WQNLEADIKE KYISERKKLY
110 120 130 140 150
SEYQKAKKEF DEKLPPKKPA GPFIKYANEV RSQVFAQHPD KSQLDLMKII
160 170 180
GDKWQSLDQS IKDKYIQEYK KAIQEYNARY PLN
Length:183
Mass (Da):21,562
Last modified:July 1, 1993 - v1
Checksum:iD280A7D2B675B5B1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M73753 Genomic DNA. Translation: AAA73079.2. Sequence problems.
AJ223169 mRNA. Translation: CAA11146.1.
Z48952 Genomic DNA. Translation: CAA88797.1.
AY557967 Genomic DNA. Translation: AAS56293.1.
BK006946 Genomic DNA. Translation: DAA09970.1.
PIRiA41302.
RefSeqiNP_013788.1. NM_001182570.1.

Genome annotation databases

EnsemblFungiiYMR072W; YMR072W; YMR072W.
GeneIDi855094.
KEGGisce:YMR072W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M73753 Genomic DNA. Translation: AAA73079.2 . Sequence problems.
AJ223169 mRNA. Translation: CAA11146.1 .
Z48952 Genomic DNA. Translation: CAA88797.1 .
AY557967 Genomic DNA. Translation: AAS56293.1 .
BK006946 Genomic DNA. Translation: DAA09970.1 .
PIRi A41302.
RefSeqi NP_013788.1. NM_001182570.1.

3D structure databases

ProteinModelPortali Q02486.
SMRi Q02486. Positions 42-179.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35247. 75 interactions.
DIPi DIP-5492N.
IntActi Q02486. 30 interactions.
MINTi MINT-505363.
STRINGi 4932.YMR072W.

Proteomic databases

MaxQBi Q02486.
PaxDbi Q02486.
PeptideAtlasi Q02486.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR072W ; YMR072W ; YMR072W .
GeneIDi 855094.
KEGGi sce:YMR072W.

Organism-specific databases

CYGDi YMR072w.
SGDi S000004676. ABF2.

Phylogenomic databases

eggNOGi COG5648.
GeneTreei ENSGT00730000113246.
InParanoidi Q02486.
KOi K11830.
OMAi DHICASH.
OrthoDBi EOG70KH25.

Enzyme and pathway databases

BioCyci YEAST:G3O-32774-MONOMER.

Miscellaneous databases

NextBioi 978405.
PROi Q02486.

Gene expression databases

Genevestigatori Q02486.

Family and domain databases

Gene3Di 1.10.30.10. 2 hits.
InterProi IPR009071. HMG_box_dom.
[Graphical view ]
Pfami PF00505. HMG_box. 2 hits.
[Graphical view ]
SMARTi SM00398. HMG. 2 hits.
[Graphical view ]
SUPFAMi SSF47095. SSF47095. 2 hits.
PROSITEi PS50118. HMG_BOX_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A close relative of the nuclear, chromosomal high-mobility group protein HMG1 in yeast mitochondria."
    Diffley J.F.X., Stillman B.
    Proc. Natl. Acad. Sci. U.S.A. 88:7864-7868(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 27-56.
  2. "Molecular cloning and characterization of yeast CDRP1: an HMG protein which recognizes cisplatin-damaged DNA and confers drug sensitivity."
    Sun C.L., Lee F.J., Lin-Chao S., Chao C.K.
    Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "A novel DNA-binding protein bound to the mitochondrial inner membrane restores the null mutation of mitochondrial histone Abf2p in Saccharomyces cerevisiae."
    Cho J.H., Ha S.J., Kao L.R., Megraw T.L., Chae C.-B.
    Mol. Cell. Biol. 18:5712-5723(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiABF2_YEAST
AccessioniPrimary (citable) accession number: Q02486
Secondary accession number(s): D6VZP6, Q712M5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: October 29, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3810 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3